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You searched for: Subject yellow Remove constraint Subject: yellow Journal Journal of virological methods Remove constraint Journal: Journal of virological methods Publication Year 2015 Remove constraint Publication Year: 2015 Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic

Author:
Nunes, Marcio R.T.; Vianez, João Lídio; Nunes, Keley N.B.; da Silva, Sandro Patroca; Lima, Clayton P.S.; Guzman, Hilda; Martins, Lívia C.; Carvalho, Valéria L.; Tesh, Robert B.; Vasconcelos, Pedro F.C.
Source:
Journal of virological methods 2015 v.226 pp. 40-51
ISSN:
0166-0934
Subject:
Yellow fever virus,   etc   ; animal pathogens; ecotourism; forests; genes; livestock; monitoring; prototypes; rearing; reverse transcriptase polymerase chain reaction; reverse transcription loop-mediated isothermal amplification; vaccines; Africa; Brazil; Caribbean; Central America;     Show all 16 Subjects
Abstract:
... Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinc ...
DOI:
10.1016/j.jviromet.2015.10.003

2. Development and characterization of mouse monoclonal antibodies against monomeric dengue virus non-structural glycoprotein 1 (NS1)

Author:
Gelanew, Tesfaye; Poole-Smith, B. Katherine; Hunsperger, Elizabeth
Source:
Journal of virological methods 2015 v.222 pp. 214-223
ISSN:
0166-0934
Subject:
Yellow fever virus,   etc   ; Dengue virus; West Nile virus; Western blotting; amino acids; antigen detection; blood serum; cross reaction; databases; dengue; diagnostic techniques; dissociation; enzyme-linked immunosorbent assay; glycoproteins; heat; mice; monoclonal antibodies; secondary infection; serotypes;     Show all 19 Subjects
Abstract:
... Dengue virus (DENV) nonstructural-1 (NS1) glycoprotein is useful for diagnosis of DENV infections in the first 8 days of illness with any of the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). However, NS1 diagnostics are less sensitive for secondary DENV infections so the utility of NS1 diagnostics in dengue endemic countries where there is predominantly secondary infections is being question ...
DOI:
10.1016/j.jviromet.2015.06.003

3. Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins

Author:
Stock, N.K.; Escadafal, C.; Achazi, K.; Cissé, M.; Niedrig, M.
Source:
Journal of virological methods 2015 v.222 pp. 110-116
ISSN:
0166-0934
Subject:
Yellow fever virus,   etc   ; Dengue virus; Japanese encephalitis virus; Tick-borne encephalitis virus; West Nile virus; Western blotting; antibodies; antiserum; cross reaction; diagnostic techniques; fluorescent antibody technique; mammals; pathogenesis; protein synthesis; viral proteins;     Show all 15 Subjects
Abstract:
... There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalia ...
DOI:
10.1016/j.jviromet.2015.06.006

4. Development of a protocol for the identification of tospoviruses and thrips species in individual thrips

Author:
Seepiban, Channarong; Charoenvilaisiri, Saengsoon; Kumpoosiri, Mallika; Bhunchoth, Anjana; Chatchawankanphanich, Orawan; Gajanandana, Oraprapai
Source:
Journal of virological methods 2015 v.222 pp. 206-213
ISSN:
0166-0934
Subject:
Melon yellow spot orthotospovirus,   etc   ; ribosomal RNA; watermelons; Capsicum chlorosis orthotospovirus; Frankliniella intonsa; genes; Scirtothrips dorsalis; tomatoes; Thrips palmi; Watermelon silver mottle tospovirus; antibodies; DNA; nucleocapsid; internal transcribed spacers; viruses; sodium; cucumbers; protocols; proteins; reverse transcriptase polymerase chain reaction; Thailand;     Show all 21 Subjects
Abstract:
... A protocol for identifying tospovirus and thrips species in an individual thrips sample was successfully developed. First, an individual thrips was soaked in an RNA stabilization solution to preserve protein and nucleic acids and ground in a carbonate buffer containing 0.2% sodium diethyldithiocarbamate. Initially, the thrips extracts were screened for tospovirus infection by dot blot analysis usi ...
DOI:
10.1016/j.jviromet.2015.06.017

5. Detection of Cucurbit chlorotic yellows virus from Bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (RT-LAMP) and simple template preparation

Author:
Okuda, Mitsuru; Okuda, Shiori; Iwai, Hisashi
Source:
Journal of virological methods 2015 v.221 pp. 9-14
ISSN:
0166-0934
Subject:
Cucurbit chlorotic yellows virus,   etc   ; Bemisia tabaci; Cucurbitaceae; DNA; DNA primers; RNA; disease severity; financial economics; genes; pathogens; reverse transcription loop-mediated isothermal amplification; sticky traps; transcription (genetics); turbidity; viruses;     Show all 15 Subjects
Abstract:
... Cucurbit chlorotic yellows virus (CCYV) of the genus Crinivirus within the family Closteroviridae is an emerging infectious agent of cucurbits leading to severe disease and significant economic losses. Effective detection and identification methods for this virus are urgently required. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to de ...
DOI:
10.1016/j.jviromet.2015.04.014