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You searched for: Subject "Cucurbit chlorotic yellows virus" Remove constraint Subject: "Cucurbit chlorotic yellows virus" Journal Journal of virological methods Remove constraint Journal: Journal of virological methods Subject Cucurbit chlorotic yellows virus Remove constraint Subject: Cucurbit chlorotic yellows virus Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Detection of Cucurbit chlorotic yellows virus from Bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (RT-LAMP) and simple template preparation

Author:
Okuda, Mitsuru; Okuda, Shiori; Iwai, Hisashi
Source:
Journal of virological methods 2015 v.221 pp. 9-14
ISSN:
0166-0934
Subject:
Cucurbit chlorotic yellows virus,   etc   ; Bemisia tabaci; Cucurbitaceae; DNA; DNA primers; RNA; disease severity; financial economics; genes; pathogens; reverse transcription loop-mediated isothermal amplification; sticky traps; transcription (genetics); turbidity; viruses;     Show all 15 Subjects
Abstract:
... Cucurbit chlorotic yellows virus (CCYV) of the genus Crinivirus within the family Closteroviridae is an emerging infectious agent of cucurbits leading to severe disease and significant economic losses. Effective detection and identification methods for this virus are urgently required. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to de ...
DOI:
10.1016/j.jviromet.2015.04.014

2. One-step reverse transcription loop mediated isothermal amplification assay for sensitive and rapid detection of Cucurbit chlorotic yellows virus

Author:
Wang, Zhenyue; Gu, Qinsheng; Sun, Hu; Li, Honglian; Sun, Bingjian; Liang, Xiangzhi; Yuan, Yuan; Liu, Ruili; Shi, Yan
Source:
Journal of virological methods 2014 v.195 pp. 63-66
ISSN:
0166-0934
Subject:
Cucurbit chlorotic yellows virus,   etc   ; RNA; Sweet potato chlorotic stunt virus; coat proteins; genes; leaves; melons; plant diseases and disorders; plant viruses; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription; reverse transcription loop-mediated isothermal amplification; temperature; tissues; viruses;     Show all 16 Subjects
Abstract:
... A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Cucurbit chlorotic yellows virus (CCYV). In this procedure, a set of four primers matching a total of six sequences in the coat protein gene region of CCYV was synthesized for the RT-LAMP assay using total RNA extracted from CCYV-infected melon leaf tissues, and the optimum reaction te ...
DOI:
10.1016/j.jviromet.2013.08.037

3. Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time RT-PCR

Author:
Abrahamian, Peter E.; Seblani, Rewa; Sobh, Hana; Abou-Jawdah, Yusuf
Source:
Journal of virological methods 2013 v.193 no.2 pp. 320-326
ISSN:
0166-0934
Subject:
Cucurbit chlorotic yellows virus,   etc   ; Cucurbit yellow stunting disorder virus; Cucurbitaceae; crops; genes; greenhouses; heat-shock protein 70; mixed infection; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; screening; viral load; viruses;     Show all 13 Subjects
Abstract:
... Cucurbit chlorotic yellows virus (CCYV) and Cucurbit yellow stunting disorder virus (CYSDV) are whitefly-transmitted criniviruses infecting cucurbit crops inducing similar symptoms. Single and multiplex RT-PCR protocols were developed and evaluated on cucurbit samples collected from commercial greenhouses. Primers and probes were designed from the highly conserved heat shock protein 70 homolog (Hs ...
DOI:
10.1016/j.jviromet.2013.06.004