U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Journal of virology Remove constraint Journal name: Journal of virology Publication year rev 7985-2015 Remove constraint Publication year rev: 7985-2015 Source 2015 v.89 no.9 Remove constraint Source: 2015 v.89 no.9
Start Over

Search

1  Search Results

1 entry found
Number of results to display per page

Search Results

1. Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus

Author:
Shin-Yi Lee Marzano; Houston A. Hobbs; Berlin D. Nelson; Glen L. Hartman; Darin M. Eastburn; Nancy K. McCoppin; Leslie L. Domier
Source:
Journal of virology 2015 v.89 no.9 pp. 5060-5071
ISSN:
0022-538X
Subject:
Glycine max; Hypovirus; Lactuca sativa; Sclerotinia sclerotiorum; complementary DNA; cycloheximide; fungal diseases of plants; genome; high-throughput nucleotide sequencing; hyphae; lettuce; messenger RNA; mycoviruses; nucleotide sequences; nucleotides; plant pathogenic fungi; rapid amplification of cDNA ends; sclerotia; soybeans; transfection; virulence
Abstract:
... A recombinant strain of Sclerotinia sclerotiorum hypovirus 2 (SsHV2) was identified from a North American Sclerotinia sclerotiorum isolate (328) from lettuce (Lactuca sativa L.) by high-throughput sequencing of total RNA. The 5′- and 3′-terminal regions of the genome were determined by rapid amplification of cDNA ends. The assembled nucleotide sequence was up to 92% identical to two recently repor ...
Handle:
10113/61160
DOI:
10.1128/JVI.03199-14
PubMed:
25694604
PubMed Central:
PMC4403457