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- Author:
- Christiano R.R. Alves; Daniele de P. Faria; Camila de G. Carneiro; Alexandre T. Garcez; Vanessa P. Gutierrez; Willian das Neves; Ney R. de Almeida; Yara Cury; Roger Chammas; Patricia C. Brum
- Source:
- Life sciences 2018 v.206 pp. 29-34
- ISSN:
- 0024-3205
- Subject:
- animal models; computed tomography; diphosphonates; disease course; femur; image analysis; longitudinal studies; neoplasm cells; neoplasms; photons; positron-emission tomography; rats; somatosensory disorders; tracer techniques
- Abstract:
- ... Noninvasive imaging using positron emission tomography/computed tomography (PET/CT) and single photon emission computed tomography/computed tomography (SPECT/CT) are considered revolutionized approaches to detect bone cancer. Both PET/CT and SPECT/CT technologies have advanced to permit miniaturization, which has provided the advantage of including animals as their own controls in longitudinal stu ...
- DOI:
- 10.1016/j.lfs.2018.05.030
- https://doi.org/10.1016/j.lfs.2018.05.030
- Author:
- Qian Zhou; Chaoqun You; Cong Zheng; Yawen Gu; Hongchao Gu; Rui Zhang; Hongshuai Wu; Baiwang Sun
- Source:
- Life sciences 2018 v.206 pp. 1-9
- ISSN:
- 0024-3205
- Subject:
- DNA; DNA damage; adverse effects; antineoplastic activity; antineoplastic agents; apoptosis; bacteriophages; binding capacity; bioavailability; breast neoplasms; cell cycle checkpoints; cell growth; cell proliferation; circular dichroism spectroscopy; dose response; enzyme inhibition; fluorescence microscopes; growth retardation; humans; interphase; mechanism of action; neoplasm cells; therapeutics; ultraviolet-visible spectroscopy
- Abstract:
- ... DNA is considered to be one of the most promising targets for anticancer agents. Acridine analogues have anticancer activity based on DNA binding and topoisomerases inhibition. However, due to the side effects, resistance and low bioavailability, a few have entered into clinical usage and the mechanisms of action are not fully understood. Novel acridine derivatives are needed for effective cancer ...
- DOI:
- 10.1016/j.lfs.2018.05.010
- https://doi.org/10.1016/j.lfs.2018.05.010
- Author:
- Chunyan Zhang; Linsen Xie; Fangxia Guan; Yuanbo Cui
- Source:
- Life sciences 2018 v.213 pp. 74-81
- ISSN:
- 0024-3205
- Subject:
- Alzheimer disease; Western blotting; amyloid; apoptosis; calcium; caspase-3; cell viability; flow cytometry; lactate dehydrogenase; neurons; neuroprotective effect; phosphatidylinositol 3-kinase; protein content; protein kinases; rats; reactive oxygen species
- Abstract:
- ... Increasing evidence displays that deposition of aggregated β-amyloid (Aβ) leads to neuronal cell apoptosis, thus aggravates the pathological progression of Alzheimer's disease (AD). 3H-1,2-dithiole-3-thione (D3T) has been proved to exert neuroprotective effects. However, the effect of D3T on protecting against Aβ-induced apoptosis and the underlying mechanism are unknown.MTT, DCFH-DA assay, LDH re ...
- DOI:
- 10.1016/j.lfs.2018.10.025
- https://doi.org/10.1016/j.lfs.2018.10.025
- Author:
- Yubo Xiao; Cheng Tian; Tao Huang; Bing Han; Meimei Wang; Hang Ma; Zhaoxing Li; Xiaoli Ye; Xuegang Li
- Source:
- Life sciences 2018 v.192 pp. 259-269
- ISSN:
- 0024-3205
- Subject:
- cell cycle checkpoints; caspases; cell viability; body weight; humans; epithelial cells; lung neoplasms; berberine; flow cytometry; Western blotting; mice; hydrophobicity; oral administration; bromides; apoptosis; cyclins
- Abstract:
- ... This study is aimed at detecting the anti-tumor efficacy of a new berberine (BBR) derivative 8-cetylberberine (HBBR), which has a significant improvement in hydrophobicity and pharmacological effects compared to BBR.The human non-small lung cancer cell line A549 and normal human lung epithelial cells (MRC-5) were cultured to observe inhibition in vitro. Cell viability was analyzed via 3-(4,5-dimet ...
- DOI:
- 10.1016/j.lfs.2017.11.012
- https://doi.org/10.1016/j.lfs.2017.11.012
- Author:
- Si-Si Wang; Li-Juan Wu; Jia-Jiang-Hui Li; Huan-Bo Xiao; Yan He; Yu-Xiang Yan
- Source:
- Life sciences 2018 v.215 pp. 170-181
- ISSN:
- 0024-3205
- Subject:
- Internet; bioinformatics; biomarkers; blood sampling; blood serum; coronary disease; databases; gene expression regulation; meta-analysis; microRNA; models; myocardial infarction; odds ratio; tissues
- Abstract:
- ... To combine the results of dysregulated miRNAs in individual coronary heart disease (CHD) studies and to identify potential miRNA biomarkers.MiRNA profiling studies of CHD were extracted from Pubmed, Embase, Web of Science and China National Knowledge Internet (CNKI) databases if they met the inclusion criteria. The meta-analysis was conducted using a random effects model to identify the effect of ...
- DOI:
- 10.1016/j.lfs.2018.11.016
- https://doi.org/10.1016/j.lfs.2018.11.016
- Author:
- Dengqiu Xu; Lu Wang; Zhenzhou Jiang; Guolin Zhao; Hozeifa M. Hassan; Lixin Sun; Sisi Fan; Zhixing Zhou; Luyong Zhang; Tao Wang
- Source:
- Life sciences 2018 v.209 pp. 313-323
- ISSN:
- 0024-3205
- Subject:
- blood glucose; blood serum; catalpol; genes; glucose; glycemic effect; histology; insulin resistance; metformin; mice; muscle development; myogenin; myosin heavy chains; noninsulin-dependent diabetes mellitus; phosphatidylinositol 3-kinase; protein synthesis; skeletal muscle; small interfering RNA; therapeutics
- Abstract:
- ... Enhancing myogenesis has been identified as a possible target to improve insulin sensitivity and protect against metabolic diseases. Catalpol, an iridoid glycoside, has been shown to exert a hypoglycaemic effect by improvement of insulin sensitivity; however, the underlying mechanism remains unknown. In this study, we tested whether catalpol has the potential to improve insulin sensitivity by augm ...
- DOI:
- 10.1016/j.lfs.2018.08.028
- https://doi.org/10.1016/j.lfs.2018.08.028
- Author:
- Beatrice Rangone; Beatrice Ferrari; Valentina Astesana; Irene Masiello; Paola Veneroni; Ilaria Zanellato; Domenico Osella; Maria Grazia Bottone
- Source:
- Life sciences 2018 v.210 pp. 166-176
- ISSN:
- 0024-3205
- Subject:
- Western blotting; adverse effects; antineoplastic activity; cell cycle; cell death; cell viability; children; cisplatin; drug resistance; drug therapy; enzyme inhibitors; fetus; flow cytometry; fluorescence microscopy; growth retardation; histone deacetylase; immunocytochemistry; ligands; neoplasms; neurons; octanoic acid; platinum; protein synthesis; rats; transmission electron microscopes; viability assays
- Abstract:
- ... Neuroblastoma is a rare cancer that affects children, mostly under the age of 5. This type of cancer starts in very early forms of immature nerve cells or developing cells found in embryo or fetus. To date cisplatin represents one of the most potent antitumor agent known, however, the onset of systemic side effects and the induction of drug resistance limit its use in the clinic for long-term trea ...
- DOI:
- 10.1016/j.lfs.2018.08.048
- https://doi.org/10.1016/j.lfs.2018.08.048
- Author:
- Yu-te Yang; Jia-hong Meng; Bin Hu; Chi-yuan Ma; Chen-chen Zhao; Wang-si-yuan Teng; Jian-qiao Hong; Si-hao Li; Guang-yao Jiang; Cong Wang; Chen-he Zhou; Shi-gui Yan
- Source:
- Life sciences 2018 v.209 pp. 409-419
- ISSN:
- 0024-3205
- Subject:
- T-lymphocytes; biomechanics; bone formation; bone resorption; cathepsin K; collagen; females; histology; mice; micro-computed tomography; mineralization; osteoblasts; osteoclasts; osteoporosis; ovariectomy; postmenopause; rutin; transcription factors
- Abstract:
- ... Postmenopausal osteoporosis is a bone metabolism disease that is caused by an imbalance between bone-resorbing osteoclast and bone-forming osteoblast actions. Herein, we describe the role of troxerutin (TRX), a trihydroxyethylated derivative of rutin, in ovariectomy (OVX)-induced osteoporosis and its effects on the regulation of osteoclasts and osteoblasts.In vivo, OVX female mice were intraperito ...
- DOI:
- 10.1016/j.lfs.2018.08.014
- https://doi.org/10.1016/j.lfs.2018.08.014
- Author:
- YongKiat Wee; TianFang Wang; Yining Liu; Xiaoyan Li; Min Zhao
- Source:
- Life sciences 2018 v.211 pp. 206-214
- ISSN:
- 0024-3205
- Subject:
- carcinogenesis; databases; gene expression; gene expression regulation; guanosinetriphosphatase; humans; mitogen-activated protein kinase; neoplasms; oncogenes; signal transduction; somatic mutation
- Abstract:
- ... There has been limited research on CNVs in oncogenes and we conducted a systematic pan-cancer analysis of CNVs and their gene expression changes. The aim of the present study was to provide an insight into the relationships between gene expression and oncogenesis.We collected all the oncogenes from ONGene database and overlapped with CNVs TCGA tumour samples from Catalogue of Somatic Mutations in ...
- DOI:
- 10.1016/j.lfs.2018.09.032
- https://doi.org/10.1016/j.lfs.2018.09.032
- Author:
- Xiao-Rong Xu; Xian-Chen Meng; Xin Wang; Dong-Yan Hou; Yan-Hong Liang; Zhi-Yong Zhang; Jia-Mei Liu; Juan Zhang; Lin Xu; Hua Wang; Wen-Shu Zhao; Lin Zhang
- Source:
- Life sciences 2018 v.210 pp. 158-165
- ISSN:
- 0024-3205
- Subject:
- at-risk population; cohort studies; confidence interval; health services; heart failure; heart rate; logit analysis; mortality; patients; prognosis; quality of life; risk; systolic blood pressure
- Abstract:
- ... The present study describes the derivation and validation of the Chronic Heart Failure Severity Index (CHFSI).The CHFSI was derived using data obtained from a single-center prospective cohort study (2000–2014) that enrolled 756 patients. Logistic regression was used to identify independent predictors of mortality and quality of life over a 15-year follow-up period.The score was validated at the fi ...
- DOI:
- 10.1016/j.lfs.2018.09.005
- https://doi.org/10.1016/j.lfs.2018.09.005
- Author:
- Natália R. Costa; Rui M. Gil da Costa; Rui Medeiros
- Source:
- Life sciences 2018 v.199 pp. 188-200
- ISSN:
- 0024-3205
- Subject:
- anus; carcinogenesis; environmental factors; esophagus; genetic factors; inflammation; mortality; patients; prognosis; public health; squamous cell carcinoma; stomach; therapeutics; viruses
- Abstract:
- ... Cancers of the gastrointestinal tract (GIT) are expected to account for approximately 20% of all cancers in 2017. Apart from their high incidence, GIT cancers show high mortality rates, placing these malignancies among the most prominent public health issues of our time. Cancers of the GIT are the result of a complex interplay between host genetic factors and environmental factors and frequently a ...
- DOI:
- 10.1016/j.lfs.2018.02.025
- https://doi.org/10.1016/j.lfs.2018.02.025
12. ALDH2 attenuates early-stage STZ-induced aged diabetic rats retinas damage via Sirt1/Nrf2 pathway
- Author:
- Mengshan He; Pan Long; Weiming Yan; Tao Chen; Lunfeng Guo; Zouming Zhang; Siwang Wang
- Source:
- Life sciences 2018 v.215 pp. 227-235
- ISSN:
- 0024-3205
- Subject:
- acetaldehyde dehydrogenase; animal disease models; dark adaptation; diabetic retinopathy; dimethyl sulfoxide; electroretinography; interleukin-6; intraperitoneal injection; laboratory animals; males; neurodegenerative diseases; rats; retina; streptozotocin; stroke; superoxide dismutase; tomography; tumor necrosis factor-alpha; vascular endothelial growth factors
- Abstract:
- ... Acetaldehyde dehydrogenase 2 (ALDH2) was reported for its protective properties on myocardial damage, stroke and neurodegeneration disease, but the effects and mechanisms of ALDH2 in the modulation of diabetic retinopathy remain unclear. The present study evaluated the protection effects of ALDH2 on streptozocin (STZ)-induced aged diabetic rats retinas damage.24 aged male diabetic Sprague-Dawley ( ...
- DOI:
- 10.1016/j.lfs.2018.10.019
- https://doi.org/10.1016/j.lfs.2018.10.019
- Author:
- Jacob Dohl; Jonathan Foldi; Julian Heller; Heath G. Gasier; Patricia A. Deuster; Tianzheng Yu
- Source:
- Life sciences 2018 v.211 pp. 238-244
- ISSN:
- 0024-3205
- Subject:
- acclimation; apoptosis; blood serum; cattle; cell culture; cell growth; culture media; diabetes mellitus; gene expression regulation; glucose; hyperlipidemia; lipids; liquids; membrane potential; mice; micelles; mitochondria; mitogen-activated protein kinase kinase; myoblasts; palmitates; protein synthesis; rats; reactive oxygen species; transcription factors
- Abstract:
- ... The interplay between hyper-glycemia and -lipidemia in diabetes mellitus (DM) is important in simulating diabetic conditions. However, cell culture media typically contain supraphysiological levels of glucose to stimulate cellular growth, which also desensitizes cells to elevated glucose levels. Moreover, creating hyperlipidemic conditions in vitro requires specialized carriers because unbound lip ...
- DOI:
- 10.1016/j.lfs.2018.09.041
- https://doi.org/10.1016/j.lfs.2018.09.041
- Author:
- Ming-Xing Wang; Jing-Xia Zhao; Yu-Jiao Meng; Ting-ting Di; Xiao-long Xu; Xiang-Jiang Xie; Yan Lin; Lu Zhang; Ning Wang; Ping Li; Yan Wang
- Source:
- Life sciences 2018 v.207 pp. 90-104
- ISSN:
- 0024-3205
- Subject:
- T-lymphocytes; Toll-like receptor 8; Western blotting; animal models; anti-inflammatory activity; dendritic cells; flow cytometry; gene expression; inflammation; interleukin-12; interleukin-23; messenger RNA; methotrexate; mice; psoriasis; reverse transcriptase polymerase chain reaction; secretion; signal transduction; skin lesions; staining; transcription (genetics)
- Abstract:
- ... Psoriasis vulgaris is mediated by T and dendritic cells. This study aimed to investigate the effects of acetyl-11-keto-β-boswellic acid (AKBA) on activated dendritic cells (DCs) using an imiquimod (IMQ)-induced psoriasis-like mouse model and murine bone marrow–derived dendritic cells (BMDCs) stimulated with resiquimod (R848) in vitro.The mice were treated with IMQ and intragastrically administered ...
- DOI:
- 10.1016/j.lfs.2018.05.044
- https://doi.org/10.1016/j.lfs.2018.05.044
- Author:
- Cui Zhai; Wenhua Shi; Wei Feng; Yanting Zhu; Jian Wang; Shaojun Li; Xin Yan; Qingting Wang; Qianqian Zhang; Limin Chai; Cong Li; Pengtao Liu; Manxiang Li
- Source:
- Life sciences 2018 v.208 pp. 87-95
- ISSN:
- 0024-3205
- Subject:
- AMP-activated protein kinase; autophagy; cytosol; heart diseases; human diseases; hypertension; hypertrophy; immunoblotting; metformin; models; monocrotaline; neoplasms; protein content; pulmonary artery; rats; transcription factor NF-kappa B
- Abstract:
- ... It has been shown that activation of autophagy is involved in the development of pulmonary arterial hypertension (PAH). Meanwhile, activation of nuclear factor-kappaB (NF-κB) has been found to induce autophagy in several types of human diseases including cancer and cardiac diseases. However, it is still unknown whether NF-κB mediates autophagy activation in PAH, and whether activation of adenosine ...
- DOI:
- 10.1016/j.lfs.2018.07.018
- https://doi.org/10.1016/j.lfs.2018.07.018
- Author:
- Thomas Stueber; Susanne Meyer; Annette Jangra; Axel Hage; Mirjam Eberhardt; Andreas Leffler
- Source:
- Life sciences 2018 v.194 pp. 67-74
- ISSN:
- 0024-3205
- Subject:
- acetaminophen; agonists; analgesia; benzoquinones; calcium; capsaicin; cell death; central nervous system; cysteine; cytotoxicity; heat; humans; image analysis; imines; metabolites; neurons; propidium; rabbits; rats; staining; synergism
- Abstract:
- ... The anandamide reuptake inhibitor N-arachidonoylaminophenol (AM404) and the reactive substance N-acetyl-p-benzoquinone imine (NAPQI) are both metabolites of acetaminophen and may contribute to acetaminophen-induced analgesia by acting at TRPV1 expressed in the peripheral or central nervous system. While NAPQI slowly sensitizes and activates TRPV1 by interacting with distinct intracellular cysteine ...
- DOI:
- 10.1016/j.lfs.2017.12.024
- https://doi.org/10.1016/j.lfs.2017.12.024
- Author:
- Viktória Kovács; Valéria Tóth-Szűki; János Németh; Viktória Varga; Gábor Remzső; Ferenc Domoki
- Source:
- Life sciences 2018 v.192 pp. 1-8
- ISSN:
- 0024-3205
- Subject:
- Western blotting; apoptosis; asphyxia; cerebral cortex; death; encephalopathy; hippocampus; hypothermia; mitogen-activated protein kinase; models; neonates; neurons; neuropathology; phosphorylation; piglets; signal transduction; translation (genetics)
- Abstract:
- ... Perinatal asphyxia (PA) often results in hypoxic–ischemic encephalopathy (HIE) in term neonates. Introduction of therapeutic hypothermia improved HIE outcome, but further neuroprotective therapies are still warranted. The present study sought to determine the feasibility of the activation of the cytoprotective PI-3-K/Akt and the MAPK/ERK signaling pathways in the subacute phase of HIE development ...
- DOI:
- 10.1016/j.lfs.2017.11.015
- https://doi.org/10.1016/j.lfs.2017.11.015
- Author:
- Diogo Farias Riberio; Paola Sanchez Cella; Lilian Eslaine Costa Mendes da Silva; Alceu Afonso Jordao; Rafael Deminice
- Source:
- Life sciences 2018 v.196 pp. 63-68
- ISSN:
- 0024-3205
- Subject:
- S-adenosylmethionine; blood; blood sampling; body weight; caudal vein; exercise; genes; homocysteine; liver; messenger RNA; metabolism; rats; sulfurtransferases; swimming; tail
- Abstract:
- ... We aimed to determine the effects of different intensities of acute exercise on Hcy plasma levels, and the exercise-induced changes in Hcy liver metabolism.First, thirty-two Wistar rats were randomly submitted to an acute bout of swimming exercise carrying a load of 2% (n=8), 4% (n=8) and 6% (n=8) of their total body weight attached in their tail. Control rats remained rested (n=8). Blood samples ...
- DOI:
- 10.1016/j.lfs.2018.01.003
- https://doi.org/10.1016/j.lfs.2018.01.003
- Author:
- Peter J. Ferrandi; Brandon G. Fico; Michael Whitehurst; Michael C. Zourdos; Fanchen Bao; Katelyn M. Dodge; Alexandra L. Rodriguez; Gabriel Pena; Chun-Jung Huang
- Source:
- Life sciences 2018 v.202 pp. 161-166
- ISSN:
- 0024-3205
- Subject:
- DNA; adipocytes; blood sampling; exercise; inflammation; interleukin-6; lipids; macrophages; males; obesity; oxidative stress
- Abstract:
- ... Obesity is associated with lipid aggregation in adipocytes and macrophage infiltration, leading to increased oxidative stress and inflammation. Increased cell-free DNA (cfDNA) concentrations have been observed in clinical conditions of systemic inflammation. While the beneficial effects of regular physical activity on the release of circulating cfDNA still remain unknown, acute intense exercise ha ...
- DOI:
- 10.1016/j.lfs.2018.04.007
- https://doi.org/10.1016/j.lfs.2018.04.007
- Author:
- Serena Benedetti; Simona Catalani; Francesco Palma; Barbara Canonico; Francesca Luchetti; Rossella Galati; Stefano Papa; Serafina Battistelli
- Source:
- Life sciences 2018 v.215 pp. 80-85
- ISSN:
- 0024-3205
- Subject:
- DNA fragmentation; T-lymphocytes; antiviral agents; apoptosis; caspase-3; cell lines; cisplatin; cytotoxicity; drug therapy; flow cytometry; fluorouracil; interphase; neoplasm cells; neoplasms; nuclear genome
- Abstract:
- ... Many antiviral agents have been reported to present direct cytotoxic activity in cancer, showing antiproliferative and proapoptotic effects through different mechanisms. In the present study, we took into account the cytotoxic action of the antiviral drug acyclovir (ACV) on leukemia cells, by investigating cell cycle perturbations and apoptosis induction upon drug administration to three still une ...
- DOI:
- 10.1016/j.lfs.2018.11.002
- https://doi.org/10.1016/j.lfs.2018.11.002
- Author:
- Lei Li; Zhongwei Sun; Caiming Xu; Jun Wu; Geliang Liu; Hongzhang Cui; Hailong Chen
- Source:
- Life sciences 2018 v.202 pp. 167-174
- ISSN:
- 0024-3205
- Subject:
- Adenoviridae; amylases; animal models; anti-inflammatory activity; apoptosis; blood serum; carboxylic ester hydrolases; cardiac output; cardioprotective effect; caudal vein; creatine kinase; genes; green fluorescent protein; heart; histology; inflammation; interleukins; intravenous injection; isozymes; lactate dehydrogenase; pancreas; pancreatitis; rats; sodium; tissues; troponin T
- Abstract:
- ... Severe acute pancreatitis (SAP) is a serious disease associated with systematic inflammation and multiple organs dysfunction. Soluble ST2 (sST2), a member of the Toll interleukin (IL)-1 receptor (TIR) superfamily, has been demonstrated to exert immune-regulatory and anti-inflammatory properties in several inflammation-related diseases. In this study, we investigated whether transfer of sST2 gene b ...
- DOI:
- 10.1016/j.lfs.2018.04.008
- https://doi.org/10.1016/j.lfs.2018.04.008
- Author:
- Yansong Ge; Qianzhen Zhang; Zhihui Jiao; Hui Li; Ge Bai; Hongbin Wang
- Source:
- Life sciences 2018 v.214 pp. 62-69
- ISSN:
- 0024-3205
- Subject:
- alanine transaminase; aspartate transaminase; autophagy; bilirubin; blood serum; hepatectomy; lactate dehydrogenase; liver; malondialdehyde; mechanism of action; medicine; miniature swine; models; myeloperoxidase; oxidative stress; parenchyma (animal tissue); postoperative complications; stem cells; superoxide dismutase; swine
- Abstract:
- ... Hepatic ischemia-reperfusion (I/R) injury is an unavoidable complication of liver surgery, often resulting in postoperative complications and liver dysfunction. Adipose-derived stem cells (ADSCs) have been recognized as a potential tool to be exploited in the field of regenerative medicine for the treatment of a variety of diseases. In the present study, the efficacy of ADSCs in a porcine I/R mode ...
- DOI:
- 10.1016/j.lfs.2018.10.054
- https://doi.org/10.1016/j.lfs.2018.10.054
- Author:
- C.R. Muller; A.L.V. Américo; P. Fiorino; F.S. Evangelista
- Source:
- Life sciences 2018 v.211 pp. 140-146
- ISSN:
- 0024-3205
- Subject:
- biochemical pathways; cafeteria foods; exercise; food intake; kidneys; lipid peroxidation; lipids; metabolism; mice; osmolarity; potassium; urine
- Abstract:
- ... The objective of this study was to investigate the potential of aerobic exercise training (AET) to prevent kidney lipid accumulation and the contribution of renal metabolism to mediate this response.Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 13), CHOW-TR (chow diet, trained; n = 13), CAF-SED (cafeteria diet, sedentary; n = 13) and CAF-TR (cafeteria diet, train ...
- DOI:
- 10.1016/j.lfs.2018.09.017
- https://doi.org/10.1016/j.lfs.2018.09.017
24. Agmatine attenuates rhabdomyolysis-induced acute kidney injury in rats in a dose dependent manner
- Author:
- Maha H. Sharawy; Rehab S. Abdelrahman; Dalia H. El-Kashef
- Source:
- Life sciences 2018 v.208 pp. 79-86
- ISSN:
- 0024-3205
- Subject:
- agmatine; albumins; animal models; arginine; blood serum; creatine; creatinine; dose response; glutathione; glycerol; heme oxygenase (biliverdin-producing); histopathology; inducible nitric oxide synthase; inflammation; interleukin-1beta; kidneys; malondialdehyde; metabolites; muscles; myeloperoxidase; neutrophils; nitric oxide; oxidative stress; protective effect; rats; rhabdomyolysis; superoxide dismutase; transcription factor NF-kappa B; tumor necrosis factor-alpha; urea nitrogen
- Abstract:
- ... Acute kidney injury (AKI) is a serious disorder that accompanies rhabdomyolysis (RM). The underlying mechanisms as well as protective approaches need to be investigated. This study was targeted to explore the prophylactic effect of agmatine (AGM), an endogenous metabolite of l-arginine against RM-induced AKI. A rat model was elucidated by 50% glycerol (10 ml/kg, im). Glycerol induced functional an ...
- DOI:
- 10.1016/j.lfs.2018.07.019
- https://doi.org/10.1016/j.lfs.2018.07.019
25. Agomelatine-induced modulation of brain-derived neurotrophic factor (BDNF) in the rat hippocampus
- Author:
- Yanxia Lu; Cyrus S. Ho; Roger S. McIntyre; Wei Wang; Roger C. Ho
- Source:
- Life sciences 2018 v.210 pp. 177-184
- ISSN:
- 0024-3205
- Subject:
- absorbance; agonists; antagonists; antidepressants; hippocampus; immunohistochemistry; laboratory animals; melatonin; neurons; protein synthesis; rats; rearing; serotonin; staining; sucrose
- Abstract:
- ... Brain-derived neurotrophic factor (BDNF) is a neurotrophin that serves as a survival factor for neurons. Agomelatine is a novel antidepressant as well as a potent agonist of melatonin (MT), MT1 and MT2 receptor types and an antagonist of the serotonin (5HT), 5-HT2C receptor. The study herein established whether treatment with agomelatine alters hippocampal BDNF protein expression under chronic unp ...
- DOI:
- 10.1016/j.lfs.2018.09.003
- https://doi.org/10.1016/j.lfs.2018.09.003
- Author:
- Jingwen Liao; Yanyan Zhang; Ying Wu; Fanxing Zeng; Lijun Shi
- Source:
- Life sciences 2018 v.199 pp. 71-79
- ISSN:
- 0024-3205
- Subject:
- animal disease models; arterioles; exercise; histology; hypertension; insulin receptor substrate proteins; insulin-like growth factor I receptor; mesenteric arteries; messenger RNA; myocytes; non-specific serine/threonine protein kinase; osteopontin; phenotype; phosphorylation; rats; smooth muscle; transfection
- Abstract:
- ... This study investigated whether long-term exercise can influence vascular smooth muscle cells (VSMCs) phenotypic switching in mesenteric arteries of hypertensive rats, with a focus on the modulation of protein kinase B (PKB/Akt) signaling by microRNA-145 (miR-145). In the exercise intervention experiment, mesenteric arteries from 3-month-old spontaneously hypertensive rats (SHR) and Wistar Kyoto r ...
- DOI:
- 10.1016/j.lfs.2018.03.011
- https://doi.org/10.1016/j.lfs.2018.03.011
27. Alamandine attenuates sepsis-associated cardiac dysfunction via inhibiting MAPKs signaling pathways
- Author:
- Peng Li; Xi-Ru Chen; Fei Xu; Chi Liu; Chang Li; Hui Liu; Hui Wang; Wei Sun; Yan-Hui Sheng; Xiang-Qing Kong
- Source:
- Life sciences 2018 v.206 pp. 106-116
- ISSN:
- 0024-3205
- Subject:
- adenosine monophosphate; apoptosis; autophagy; blood pressure; calcium-binding proteins; cardiomyocytes; cyclic AMP; death; endothelial nitric oxide synthase; inducible nitric oxide synthase; inflammation; lipopolysaccharides; mice; mitogen-activated protein kinase; myosin; sepsis (infection); signal transduction
- Abstract:
- ... Sepsis-induced myocardial dysfunction represents a major cause of death. Alamandine is an important biologically active peptide. The present study evaluated whether alamandine improves cardiac dysfunction, inflammation, and apoptosis, and affects the signaling pathways involved in these events. Experiments were carried out in mice treated with lipopolysaccharide (LPS) or alamandine, and in neonata ...
- DOI:
- 10.1016/j.lfs.2018.04.010
- https://doi.org/10.1016/j.lfs.2018.04.010
- Author:
- Riyoko Tamai; Yusuke Kiyoura
- Source:
- Life sciences 2018 v.198 pp. 8-17
- ISSN:
- 0024-3205
- Subject:
- Gram-negative bacteria; adverse effects; antibodies; bone resorption; caspase-1; caspase-8; cell death; enzyme inhibitors; interleukin-1beta; interleukin-6; lipid A; tumor necrosis factor-alpha
- Abstract:
- ... Alendronate (ALN) is a nitrogen-containing bisphosphonate (NBP) that inhibits bone resorption. NBPs have inflammatory side effects, and ALN augments bacteria-induced interleukin (IL)-1β production. The present study aimed to examine whether ALN induces pyroptosis, a form of cell death associated with IL-1β release, in macrophage-like J774.1 cells incubated with lipid A, a component of gram-negativ ...
- DOI:
- 10.1016/j.lfs.2018.02.014
- https://doi.org/10.1016/j.lfs.2018.02.014
- Author:
- Muhammed A. Saad; Alyasaa A. Rastanawi; Muhammed F. El-Yamany
- Source:
- Life sciences 2018 v.215 pp. 11-21
- ISSN:
- 0024-3205
- Subject:
- acetaminophen; acetylcholine; acetylcholinesterase; actin; alanine transaminase; alkaline phosphatase; ammonia; apoptosis; aspartate transaminase; autophagy; bioavailability; blood; caspase-3; cytochrome c; dopamine; encephalopathy; enzyme activity; gamma-aminobutyric acid; glutamic acid; hepatocytes; hepatotoxicity; hippocampus; histopathology; inflammation; ligands; liver; memory; memory disorders; muscles; neurotransmitters; norepinephrine; poisoning; rats; serotonin; transforming growth factor beta 1; tumor necrosis factor-alpha
- Abstract:
- ... Hepatic encephalopathy (HE) is a complex neuropsychiatric syndrome. Paracetamol (APAP) causes, in high doses, a hepatic injury. Alogliptin (ALO), with its 100% oral bioavailability, may be able to reverse the acute hepatic injury and memory impairments.Forty rats were divided into four groups as follows; Normal Control Group, APAP intoxicated group, ALO and SIL groups. Behavioral tests (Morris wat ...
- DOI:
- 10.1016/j.lfs.2018.10.069
- https://doi.org/10.1016/j.lfs.2018.10.069
- Author:
- Ramasamy Karthikeyan; Govindasamy Kanimozhi; Nagarajan Rajendra Prasad; Balupillai Agilan; Muthusamy Ganesan; Gunaseelan Srithar
- Source:
- Life sciences 2018 v.212 pp. 150-158
- ISSN:
- 0024-3205
- Subject:
- DNA; DNA damage; DNA repair; alpha-pinene; antioxidant activity; apoptosis; cytotoxicity; essential oils; fluorescence emission spectroscopy; fluorescence microscopy; inflammation; interleukin-6; keratinocytes; lipid peroxidation; membrane potential; mitochondrial membrane; oxidative stress; protective effect; skin (animal); transcription factor NF-kappa B; tumor necrosis factor-alpha; ultraviolet radiation
- Abstract:
- ... This study aims to evaluate the protective effect of alpha pinene (AP), an essential oil monoterpene, against ultraviolet-A (UVA; 320–400 nm) induced cellular damages in human skin epidermal keratinocytes (HaCaT cells).In this study, HaCaT cells were subjected to single UVA-irradiation (10 J/cm²) in the presence and absence of AP (30 μM) then different cellular end points were analyzed. The protec ...
- DOI:
- 10.1016/j.lfs.2018.10.004
- https://doi.org/10.1016/j.lfs.2018.10.004
- Author:
- Anna Kothencz; Judit Hajagos-Tóth; Adrienn Csányi; Róbert Gáspár
- Source:
- Life sciences 2018 v.192 pp. 199-204
- ISSN:
- 0024-3205
- Subject:
- alpha-tocopherol; diclofenac; enzyme activity; enzyme inhibitors; lipids; myometrium; prostaglandin synthase; rats; risk; succinic acid
- Abstract:
- ... Lipid soluble vitamin E plays a role in several physiological mechanisms, however, the mechanism of this action is controversial. We investigated how tocopherol (α-tocopherol acid succinate) influences the effects of cyclooxygenase inhibitors (COXi) in the smooth muscles.The contractility of the samples from 22-day-pregnant myometrium and non-pregnant myometrium and trachea was determined in an is ...
- DOI:
- 10.1016/j.lfs.2017.11.048
- https://doi.org/10.1016/j.lfs.2017.11.048
- Author:
- Maria Florencia Albertoni Borghese; Sandra Hope; Maria del Carmen Ortiz; Magalí Barchuk; Camila Kessler; Carlos Davio; Marcelo Vatta; Mónica Majowicz
- Source:
- Life sciences 2018 v.208 pp. 72-78
- ISSN:
- 0024-3205
- Subject:
- cyclic AMP; cytosol; excretion; fluorescent antibody technique; hypertension; immunohistochemistry; kidneys; models; patients; quantitative polymerase chain reaction; rats; vasopressin
- Abstract:
- ... The aim of the present study was to evaluate the regulation of Aquaporin-2 (AQP2) water channel in the kidney of one-kidney, one-clip rats (Goldblatt-1 model). In addition, some mechanisms that underlie the role of AQP2 in the Goldblatt-1 model were evaluated.Sprague-Dawley rats were divided in three groups: control two-kidney, no clip (C, 2 K-NC); nephrectomized one-kidney, no clip (N, 1 K-NC) an ...
- DOI:
- 10.1016/j.lfs.2018.07.020
- https://doi.org/10.1016/j.lfs.2018.07.020
- Author:
- Kevin E. McElhanon; Sayak Bhattacharya
- Source:
- Life sciences 2018 v.192 pp. 166-172
- ISSN:
- 0024-3205
- Subject:
- homeostasis; models; muscles; muscular diseases; myocardium; researchers
- Abstract:
- ... Sarcolemmal integrity is orchestrated through the interplay of preserving membrane strength and fast tracking the membrane repair process during an event of compromised membrane fragility. Several molecular players have been identified that act in a concerted fashion to maintain the barrier function of the muscle membrane. Substantial research findings in the field of muscle biology point out the ...
- DOI:
- 10.1016/j.lfs.2017.11.035
- https://doi.org/10.1016/j.lfs.2017.11.035
- Author:
- Carissa C. James; James W. Smyth
- Source:
- Life sciences 2018 v.212 pp. 138-144
- ISSN:
- 0024-3205
- Subject:
- human diseases; hypertrophy; internal ribosome entry sites; mammals; messenger RNA; neoplasms; open reading frames; proteome; ribosomes; start codon; therapeutics; translation (genetics)
- Abstract:
- ... Eukaryotic mRNAs were historically thought to rely exclusively on recognition and binding of their 5′ cap by initiation factors to effect protein translation. While internal ribosome entry sites (IRESs) are well accepted as necessary for the cap-independent translation of many viral genomes, there is now recognition that eukaryotic mRNAs also undergo non-canonical modes of translation initiation. ...
- DOI:
- 10.1016/j.lfs.2018.09.054
- PubMed:
- 30290184
- PubMed Central:
- PMC6345546
- https://doi.org/10.1016/j.lfs.2018.09.054
- Author:
- Natalie Cortes; Carol Castañeda; Edison H. Osorio; Gloria Patricia Cardona-Gomez; Edison Osorio
- Source:
- Life sciences 2018 v.203 pp. 54-65
- ISSN:
- 0024-3205
- Subject:
- Clivia miniata; Eucharis; alkaloids; antioxidant activity; antioxidants; bulbs; electrons; enols; free radicals; leaves; mechanism of action; models; moieties; neurons; neuroprotective effect; phenol; rats
- Abstract:
- ... Determine a relationship between the neuroprotective activity and the antioxidant capacity of the Amaryllidaceae alkaloids in a model of Glu excitotoxicity in rat cortical neurons.Was evaluated several alkaloidal fractions isolated from Amaryllidaceae species, a family known to contain neuroprotective alkaloids, in a model of Glu excitotoxicity in rat cortical neurons. In addition, several mechani ...
- DOI:
- 10.1016/j.lfs.2018.04.026
- https://doi.org/10.1016/j.lfs.2018.04.026
- Author:
- Yun-Li Yu; Na-Na Li; Meng-Ting Shi; Hong-Juan Lu
- Source:
- Life sciences 2018 v.209 pp. 403-408
- ISSN:
- 0024-3205
- Subject:
- anticonvulsants; drug therapy; epilepsy; heart; heart rate; patients
- Abstract:
- ... Changes in cardiac autonomic nervous function have been evaluated by studying the related indexes of heart rate variability (HRV) in patients with focal epilepsy (FE) in the interictal period.A total of 30 FE patients who were treated in our department from July 2015 to May 2017, were included into this study. These patients were divided into three pairs of groups: less frequent seizure group and ...
- DOI:
- 10.1016/j.lfs.2018.08.033
- https://doi.org/10.1016/j.lfs.2018.08.033
- Author:
- Zhi-Meng Xu; Feng Huang; Wei-Qiang Huang
- Source:
- Life sciences 2018 v.211 pp. 157-171
- ISSN:
- 0024-3205
- Subject:
- angiogenesis; biomarkers; clinical trials; endothelial cells; mesenchymal stromal cells; myocardial ischemia; myocytes; non-coding RNA; patients; prognosis; quality of life; smooth muscle; stem cells; therapeutics
- Abstract:
- ... Long noncoding RNAs (LncRNAs) are involved in biological processes and the pathology of diseases and represent an important biomarker or therapeutic target for disease. Emerging evidence has suggested that lncRNAs modulate angiogenesis by regulating the angiogenic cell process—including vascular endothelial cells (VECs); stem cells, particularly bone marrow-derived stem cells, endothelial progenit ...
- DOI:
- 10.1016/j.lfs.2018.09.022
- https://doi.org/10.1016/j.lfs.2018.09.022
- Author:
- Caio Pupin Rosa; Gustavo Andrade Brancaglion; Tatimara M. Miyauchi-Tavares; Patrícia Paiva Corsetti; Leonardo Augusto de Almeida
- Source:
- Life sciences 2018 v.207 pp. 480-491
- ISSN:
- 0024-3205
- Subject:
- T-lymphocytes; animal models; antibiotics; bacteria; bacterial infections; drugs; dysbiosis; epidemiological studies; homeostasis; human health; humans; immunoglobulin A; intestinal mucosa; mice; microbiome; short chain fatty acids; translation (genetics)
- Abstract:
- ... The gastrointestinal tract has become a focus of study recently. The crosstalk between microbiota, especially bacteria, and the intestinal mucosa has to be accurately balanced in order to maintain physiological homeostasis in the human body. This dynamic interaction results in different levels of short-chain fatty acids (SCFAs), IgA, and T cell lymphocyte subsets, which could lead the human body t ...
- DOI:
- 10.1016/j.lfs.2018.06.030
- https://doi.org/10.1016/j.lfs.2018.06.030
- Author:
- S. Ramírez-Rivera; S. Pizarro; M. Gallardo; F. Gajardo; A. Delgadillo; E. De La Fuente-Ortega; F.M. MacDonnell; G. Bernal
- Source:
- Life sciences 2018 v.213 pp. 57-65
- ISSN:
- 0024-3205
- Subject:
- antineoplastic activity; apoptosis; caspases; cisplatin; confocal microscopy; cytotoxicity; enzyme activity; gene overexpression; genes; humans; neoplasm cells; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ruthenium; stomach neoplasms
- Abstract:
- ... Ruthenium (II) complexes are promising anticancer molecules due its pharmacological properties and selectivity to cells tumor. The aim of this work was to study the cytotoxic activity, and apoptosis induction of two new ruthenium complexes on a human gastric cancer cell line.Two ruthenium(II) complexes were synthesized: [(H₂pbbzim)Ru(tpy-Ph-COOCH₃)](Cl)₂ (Ru-UCN1), and [(tpy)Ru(tpy-Ph-bzH)](Cl)₂ ( ...
- DOI:
- 10.1016/j.lfs.2018.10.024
- https://doi.org/10.1016/j.lfs.2018.10.024
- Author:
- Atsuo Tahara; Toshiyuki Takasu
- Source:
- Life sciences 2018 v.197 pp. 80-90
- ISSN:
- 0024-3205
- Subject:
- animal disease models; carbohydrate content; carbohydrate intake; dietary carbohydrate; excretion; glucose; glucose tolerance; glycemic effect; high carbohydrate diet; hyperglycemia; hyperinsulinemia; insulin resistance; kidney diseases; low carbohydrate diet; mice; noninsulin-dependent diabetes mellitus; obesity; oral administration; patients; symporters
- Abstract:
- ... Daily intake of carbohydrates differs among individual patients with type 2 diabetes. Here, we investigated whether or not dietary carbohydrate content affects the efficacy of the sodium-glucose cotransporter 2 (SGLT2) inhibitor ipragliflozin in type 2 diabetic mice.Diabetic mice were fed a regular (50% kcal), high (75% kcal)-, or low (25% kcal)-carbohydrate diet. Ipragliflozin was orally administ ...
- DOI:
- 10.1016/j.lfs.2018.02.009
- https://doi.org/10.1016/j.lfs.2018.02.009
- Author:
- Chang Xian Li; Lei Lei Chen; Xiang Cheng Li; Kevin Tak-Pan Ng; Xin Xiang Yang; Chung Mau Lo; Xin Yuan Guan; Kwan Man
- Source:
- Life sciences 2018 v.215 pp. 128-135
- ISSN:
- 0024-3205
- Subject:
- animal models; apolipoprotein A-I; energy metabolism; fatty liver; hepatectomy; hepatocytes; liver; liver regeneration; liver transplant; mice; mitochondria; oleic acid; rats
- Abstract:
- ... Apolipoprotein A-1 (ApoA-1) is involved in regulating both lipid and energy metabolism, which may play important roles in liver regeneration, especially for the liver with steatosis. We here intended to investigate the role of ApoA-1 in regeneration of small-for-size fatty liver graft and to explore the underlying mechanism.The association of ApoA-1 expression with liver regeneration was studied i ...
- DOI:
- 10.1016/j.lfs.2018.10.053
- https://doi.org/10.1016/j.lfs.2018.10.053
- Author:
- Chenxu Li; Shubin Chen; Yulai Zhou; Yifan Zhao; Pengfei Liu; Jinglei Cai
- Source:
- Life sciences 2018 v.212 pp. 145-149
- ISSN:
- 0024-3205
- Subject:
- allografting; cell differentiation; embryonic stem cells; ethics; humans; immunogenicity; induced pluripotent stem cells; patients; researchers; somatic cells
- Abstract:
- ... The development of induced pluripotent stem cells (iPS cells) has raised the prospect of patient-specific treatments for various diseases. Theoretically, iPS cell technology avoids the limitations of human embryonic stem cells (ES cells), including poor establishment, ethical issues, and immune rejection of allogeneic transplantation. However, the immunogenicity of iPS cells has attracted the atte ...
- DOI:
- 10.1016/j.lfs.2018.09.057
- https://doi.org/10.1016/j.lfs.2018.09.057
- Author:
- P.S. Pavithra; Alka Mehta; Rama S. Verma
- Source:
- Life sciences 2018 v.197 pp. 19-29
- ISSN:
- 0024-3205
- Subject:
- antineoplastic activity; apoptosis; ascorbic acid; caspase-3; caspase-9; cell cycle checkpoints; cysteine; cytochrome c; cytotoxicity; essential oils; flow cytometry; humans; interphase; membrane potential; mitochondria; mitochondrial membrane; neoplasm cells; neoplasms; sesquiterpenoids; viability
- Abstract:
- ... Aromadendrene oxide 2 (AO-(2)) is an oxygenated sesquiterpene naturally found as a chemical component of essential oils. In the present study anticancer activity of AO-(2) has been investigated on A431 human epidermoid cancer and precancerous HaCaT cells.Cell viability was used to detect cytotoxic activity. Mechanism of cell death induced by AO-(2) treatments was studied using Annexin V-FITC/PI bi ...
- DOI:
- 10.1016/j.lfs.2018.01.029
- https://doi.org/10.1016/j.lfs.2018.01.029
44. Arsenic aggravates oxidative stress causing hepatic alterations and inflammation in diabetic rats
- Author:
- Ana Cláudia Ferreira Souza; Daniel Silva Sena Bastos; Felipe Couto Santos; Marcela Nascimento Sertorio; Luiz Otávio Guimarães Ervilha; Reggiani Vilela Gonçalves; Leandro Licursi de Oliveira; Mariana Machado-Neves
- Source:
- Life sciences 2018 v.209 pp. 472-480
- ISSN:
- 0024-3205
- Subject:
- animal disease models; antioxidants; arsenic; biomarkers; blood serum; diabetes; drinking water; enzymes; glycogen; hemorrhage; hemosiderin; histopathology; hyperglycemia; inflammation; liver; males; malondialdehyde; mast cells; oxidative stress; protein content; rats; sodium arsenate; sodium chloride; streptozotocin; toxic substances; tumor necrosis factor-alpha
- Abstract:
- ... Studies have shown that exposure to either environmental toxicants or hyperglycemia causes hepatic injuries. However, it is unclear the extent to which their combined exposure may influence liver functions. Therefore, we aimed to evaluate morphological and functional hepatic parameters in diabetic rats exposed to arsenic.Diabetes was induced in male rats by intraperitoneal streptozotocin injection ...
- DOI:
- 10.1016/j.lfs.2018.08.054
- https://doi.org/10.1016/j.lfs.2018.08.054
- Author:
- Wenjing Cheng; Weimei Chen; Peng Wang; Jianfeng Chu
- Source:
- Life sciences 2018 v.208 pp. 96-101
- ISSN:
- 0024-3205
- Subject:
- Alzheimer disease; IKappaB kinase; Western blotting; amyloid; apoptosis; membrane potential; mitochondrial membrane; neurotoxicity; phosphatidylinositol 3-kinase; phosphorylation; protective effect; signal transduction; staining; viability
- Abstract:
- ... Amyloid β (Aβ) peptide can cause neurotoxicity in Alzheimer's disease (AD). The main purpose of the present study is to investigate the protective role of asiatic acid (AA) against Aβ25–35-induced neurotoxicity in neuronally differentiated PC12 cells. Differentiated PC12 cells were pretreated with 5, 10 or 20 μM AA before treatment with 20 μM Aβ25–35. The viability and apoptosis of differentiated ...
- DOI:
- 10.1016/j.lfs.2018.07.016
- https://doi.org/10.1016/j.lfs.2018.07.016
- Author:
- Nancy S. Saad; Mohammad T. Elnakish; Elizabeth A. Brundage; Brandon J. Biesiadecki; Ahmet Kilic; Amany A.E. Ahmed; Peter J. Mohler; Paul M.L. Janssen
- Source:
- Life sciences 2018 v.215 pp. 119-127
- ISSN:
- 0024-3205
- Subject:
- cAMP-dependent protein kinase; cardiac output; dimethyl sulfoxide; heart failure; humans; inhibitory concentration 50; muscles; myocardium; protein kinase C; United States
- Abstract:
- ... Heart failure (HF) is a prevalent disease that is considered the foremost reason for hospitalization in the United States. Most protein kinases (PK) are activated in heart disease and their inhibition has been shown to improve cardiac function in both animal and human studies. However, little is known about the direct impact of PKA and PKC inhibitors on human cardiac contractile function.We invest ...
- DOI:
- 10.1016/j.lfs.2018.10.065
- PubMed:
- 30399377
- PubMed Central:
- PMC6279240
- https://doi.org/10.1016/j.lfs.2018.10.065
- Author:
- Kunsala T.D.S. Yapa; Jennifer Deuis; Amelia A. Peters; Paraic A. Kenny; Sarah J. Roberts-Thomson; Irina Vetter; Gregory R. Monteith
- Source:
- Life sciences 2018 v.198 pp. 128-135
- ISSN:
- 0024-3205
- Subject:
- breast neoplasms; calcium; cell lines; cell viability; electric power; membrane potential; messenger RNA; neoplasm cells; quantitative polymerase chain reaction; sequence analysis; sodium channels
- Abstract:
- ... To assess levels of the calcium permeable transient receptor potential cation channel, subfamily melastatin, member 8 (TRPM8) in breast cancer molecular subtypes and to assess the consequences of TRPM8 pharmacological inhibition with AMTB (an inhibitor of TRPM8) on breast cancer cell lines.Cell viability and migration of breast cancer cells was determined using MTS assays and wound healing assays, ...
- DOI:
- 10.1016/j.lfs.2018.02.030
- https://doi.org/10.1016/j.lfs.2018.02.030
48. Assessment of the cerebellar neurotoxic effects of nicotine in prenatal alcohol exposure in rats
- Author:
- Dwipayan Bhattacharya; Mohammed Majrashi; Sindhu Ramesh; Manoj Govindarajulu; Jenna Bloemer; Ayaka Fujihashi; Bailee-Ryan Crump; Harrison Hightower; Subhrajit Bhattacharya; Timothy Moore; Vishnu Suppiramaniam; Muralikrishnan Dhanasekaran
- Source:
- Life sciences 2018 v.194 pp. 177-184
- ISSN:
- 0024-3205
- Subject:
- adverse effects; alcohols; amine oxidase (flavin-containing); animal models; apoptosis; behavior change; biomarkers; caspases; cerebellum; enzyme activity; humans; mitochondria; neurotoxicity; nicotine; oxidative stress; pregnancy; progeny; rats; therapeutics; women
- Abstract:
- ... The adverse effects of prenatal nicotine and alcohol exposure on human reproductive outcomes are a major scientific and public health concern. In the United States, substantial percentage of women (20–25%) of childbearing age currently smoke cigarettes and consume alcohol, and only a small percentage of these individuals quit after learning of their pregnancy. However, there are very few scientifi ...
- DOI:
- 10.1016/j.lfs.2017.12.010
- https://doi.org/10.1016/j.lfs.2017.12.010
- Author:
- Zohreh Nowzari; Mohammad Masoumi; Mahdieh Nazari-Robati; Hamed Akbari; Nader Shahrokhi; Gholamreza Asadikaram
- Source:
- Life sciences 2018 v.207 pp. 166-171
- ISSN:
- 0024-3205
- Subject:
- DNA; alleles; blood; case-control studies; coronary artery disease; genetic models; genotype; heart; hypertension; leptin; leptin receptors; patients; protective effect; regression analysis; single nucleotide polymorphism
- Abstract:
- ... Apelin and leptin are factors which have a potential physiological and pathological role in cardiovascular homoeostasis. Apelin receptor (APLNR), leptin receptor (LEPR) and leptin variants may affect the vascular tone in heart or peripheral circulation, thereby predisposing patients to hypertension and coronary artery disease (CAD). The aim of the present study was to evaluate four single nucleoti ...
- DOI:
- 10.1016/j.lfs.2018.06.007
- https://doi.org/10.1016/j.lfs.2018.06.007
- Author:
- Xiaoshan Yu; Bin Zhao; Yanhua Su; Yan Zhang; Jianghui Chen; Wenhui Wu; Qijun Cheng; Xiaorong Guo; Zeyu Zhao; Xiayi Ke; Wangmu Danzeng; Benhua Zhao; Qilin Ma
- Source:
- Life sciences 2018 v.200 pp. 81-86
- ISSN:
- 0024-3205
- Subject:
- DDT (pesticide); DNA methylation; blood; epigenetics; gene expression; genes; maternal exposure; public health; tumor suppressor proteins
- Abstract:
- ... To investigate whether intrauterine organochlorine pesticide (OCP)-dichlorodiphenyltrichloroethane (DDT) exposure could lead to epigenetic alterations by DNA methylation with possible important lifetime health consequences for offspring.We used Illumina Infinium HumanMethylation 450 K BeadChip to explore the pattern of genome-wide DNA methylation containing >485,000 gene sites in cord blood of 24 ...
- DOI:
- 10.1016/j.lfs.2018.03.030
- https://doi.org/10.1016/j.lfs.2018.03.030
- Author:
- Caihong Sun; Mingyang Zou; Ling Li; Dexin Li; Yongjuan Ma; Wei Xia; Lijie Wu; Huan Ren
- Source:
- Life sciences 2018 v.213 pp. 183-189
- ISSN:
- 0024-3205
- Subject:
- Chinese people; alleles; animal models; autism; case-control studies; children; confidence interval; disease susceptibility; etiology; genetic variation; genotyping; hippocampus; odds ratio; patients; potassium; potassium channels; rats; risk; single nucleotide polymorphism; valproic acid
- Abstract:
- ... Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders involving structural and functional impairment of the brain. Inwardly rectifying potassium (Kir) channels may contribute to the etiology of ASD by altering brain function. This study investigated the associations between genetic variants of KCNJ2 and KCNJ10 genes (encoding Kir2.1 and Kir4.1, respectively) and ASD risk in p ...
- DOI:
- 10.1016/j.lfs.2018.10.012
- https://doi.org/10.1016/j.lfs.2018.10.012
- Author:
- Bin Leng; Futian Tang; Meili Lu; Zhen Zhang; Hongxin Wang; Yingjie Zhang
- Source:
- Life sciences 2018 v.209 pp. 111-121
- ISSN:
- 0024-3205
- Subject:
- Astragalus membranaceus; Toll-like receptor 4; active ingredients; antioxidant activity; astragalosides; blood serum; diabetes; endothelial nitric oxide synthase; enzyme-linked immunosorbent assay; human umbilical vein endothelial cells; hyperglycemia; immunohistochemistry; intercellular adhesion molecule-1; interleukin-6; intraperitoneal injection; medicinal properties; models; nitrate reductase; nitric oxide; protective effect; signal transduction; streptozotocin; transcription factor NF-kappa B; tumor necrosis factor-alpha; vascular cell adhesion molecules
- Abstract:
- ... Astragaloside IV (As-IV) is the major active ingredient of Astragalus membranaceus and has diverse pharmacological activities, including anti-inflammatory and antioxidant effects. However, the beneficial effect of As-IV on protecting vascular endothelial dysfunction is not completely understood. The aim of this study was to investigate the protective effect and mechanism of As-IV on vascular endot ...
- DOI:
- 10.1016/j.lfs.2018.07.053
- https://doi.org/10.1016/j.lfs.2018.07.053
- Author:
- Deng-qing Zhang; Jin-song Li; Yu-mei Zhang; Feng Gao; Ruo-zhu Dai
- Source:
- Life sciences 2018 v.200 pp. 105-109
- ISSN:
- 0024-3205
- Subject:
- Oriental traditional medicine; Western blotting; actin; adults; angiotensin II; astragalosides; atherosclerosis; cell cycle; cyclin-dependent kinase; enzyme activity; flow cytometry; muscles; myocytes; phenotype; rats; reverse transcriptase polymerase chain reaction; smooth muscle
- Abstract:
- ... Astragaloside IV (AS-IV) is the central active component extracted from Radix astragali, an herbal remedy widely used in traditional Chinese medicine for the treatment of cardiovascular diseases. Aberrant proliferation of vascular smooth muscle cells (VSMCs) is closely involved in the initiation and progression of cardiovascular complications, such as atherosclerosis. Here we investigated whether ...
- DOI:
- 10.1016/j.lfs.2018.03.036
- https://doi.org/10.1016/j.lfs.2018.03.036
- Author:
- Xiao Lei; Bo-da Zhang; Ji-gang Ren; Fang-li Luo
- Source:
- Life sciences 2018 v.206 pp. 77-83
- ISSN:
- 0024-3205
- Subject:
- Western blotting; albuminuria; animal disease models; apoptosis; astragalosides; diabetic nephropathy; enzyme-linked immunosorbent assay; glucose; luciferase; messenger RNA; quantitative polymerase chain reaction; rats; reverse transcriptase polymerase chain reaction; signal transduction; staining; streptozotocin; transfection; transferases; tumor necrosis factors
- Abstract:
- ... Apoptosis of podocytes plays a crucial role in diabetic nephropathy (DN) development, and astragaloside (AS-IV) has a significant impact on podocyte apoptosis. This study aims to explore the effect of AS-IV on diabetic nephropathy progression.The diabetic nephropathy model was established in rats with streptozotocin (STZ) injection. The albuminuria was examined by using the enzyme linked immunosor ...
- DOI:
- 10.1016/j.lfs.2018.05.037
- https://doi.org/10.1016/j.lfs.2018.05.037
- Author:
- Ambika Binesh; Sivasithamparam Niranjali Devaraj; Devaraj Halagowder
- Source:
- Life sciences 2018 v.196 pp. 28-37
- ISSN:
- 0024-3205
- Subject:
- anti-inflammatory agents; atherosclerosis; brain; diet; diosgenin; disease course; heart; kidneys; lipids; liver; pancreas; pathophysiology; rats; spleen; transcription factor NF-kappa B; tumor necrosis factor-alpha
- Abstract:
- ... Atherogenic Diet (AD) was given to rats to understand the key role of inflammatory mediators in atherosclerotic lesion formation, as a serendipitous study, the diet induced inflammatory mediators in liver and brain, whereas pancreas, kidney and spleen were not affected. The efficacy of diosgenin in ameliorating atherosclerotic progression in heart and suppression of inflammatory mediators in liver ...
- DOI:
- 10.1016/j.lfs.2018.01.012
- https://doi.org/10.1016/j.lfs.2018.01.012
- Author:
- Jiang Du; Yong Zhu; Xiaoxiao Meng; Hui Xie; Jinfeng Wang; Zhigang Zhou; Ruilan Wang
- Source:
- Life sciences 2018 v.213 pp. 126-133
- ISSN:
- 0024-3205
- Subject:
- atorvastatin; beta catenin; cadherins; cell culture; cell lines; dose response; epithelium; fibrosis; hypoxia-inducible factor 1; lungs; paraquat; rats; therapeutics; vimentin
- Abstract:
- ... This study investigated the effects of atorvastatin (ATS) on the paraquat (PQ)-induced epithelial-mesenchymal transition (EMT) and the potential mechanism through hypoxia-inducible factor-1 alpha (HIF-1α).Sprague–Dawley (SD) rats were randomly divided into a control group (n = 5), PQ group (n = 20), PQ + ATS L group (n = 20, ATS 20 mg/kg daily) and PQ + ATS H group (n = 20, ATS 40 mg/kg daily). Al ...
- DOI:
- 10.1016/j.lfs.2018.10.026
- https://doi.org/10.1016/j.lfs.2018.10.026
- Author:
- Hamed Akbari; Gholamreza Asadikaram; Ahmad Jafari; Mahdieh Nazari-Robati; Ghasem Ebrahimi; Nazanin Ebrahimi; Mohammad Masoumi
- Source:
- Life sciences 2018 v.207 pp. 525-531
- ISSN:
- 0024-3205
- Subject:
- alleles; blood serum; coronary artery disease; cytokines; enzyme-linked immunosorbent assay; gene expression regulation; genetic models; genetic polymorphism; hypertension; patients; risk reduction
- Abstract:
- ... Interleukin-22 (IL-22) may be considered as an important cytokine in maintenance and progression of hypertension and coronary artery disease (CAD). The aim of the present study was to investigate the effect of treatment of hypertension and CAD on serum levels of IL-22 and the possible association of IL-22-rs1179251 gene polymorphism with hypertension and CAD.A total of 286 subjects with suspected ...
- DOI:
- 10.1016/j.lfs.2018.07.005
- https://doi.org/10.1016/j.lfs.2018.07.005
- Author:
- Shiying Shao; Xuefeng Yu; Liya Shen
- Source:
- Life sciences 2018 v.192 pp. 160-165
- ISSN:
- 0024-3205
- Subject:
- autoimmunity; cytokines; intestinal microorganisms; lymphocytes; neutralization; signal transduction; thyroid diseases; transcription (genetics); transcription factors
- Abstract:
- ... Years of researches have demonstrated that the imbalance of Th17 and Tregs contribute to the thyroid autoimmunity and the severity of autoimmune thyroid disease (AITD). The underlying mechanism comprises inherent genetic predisposition, abnormality of Th17 and Treg related biological molecules, and gut microbiota disorder. New therapeutic strategies have been developed to improve the Th17/Treg equ ...
- DOI:
- 10.1016/j.lfs.2017.11.026
- https://doi.org/10.1016/j.lfs.2017.11.026
- Author:
- Yu Fang; Chun Liu; Bo Shu; Mimi Zhai; Chaolin Deng; Chao He; Ming Luo; Tong Han; Wei Zheng; Jingyao Zhang; Sushun Liu
- Source:
- Life sciences 2018 v.209 pp. 490-497
- ISSN:
- 0024-3205
- Subject:
- Western blotting; blood serum; cell proliferation; enzyme-linked immunosorbent assay; flow cytometry; immunohistochemistry; liver; liver regeneration; mice; mitogen-activated protein kinase; models; reverse transcriptase polymerase chain reaction; serotonin
- Abstract:
- ... Serotonin and YAP exhibit a vital role in regulating cell proliferation and wound-healing response. The aim of the study was to investigate whether 5-HT could promote liver regeneration by activating YAP.PH models were established by WT and TPH1−/− mice. ELISA, RT-PCR, western blot, immunohistochemistry, flow cytometry and MTT assay were used to assess the level of 5-HT and YAP and proliferation a ...
- DOI:
- 10.1016/j.lfs.2018.08.047
- https://doi.org/10.1016/j.lfs.2018.08.047
- Author:
- Yingying Shen; Xiguang Chen; Jun He; Duanfang Liao; Xuyu Zu
- Source:
- Life sciences 2018 v.198 pp. 99-111
- ISSN:
- 0024-3205
- Subject:
- breasts; cell proliferation; drug therapy; drugs; humans; lungs; metastasis; pancreatic neoplasms; patients; receptor protein-tyrosine kinase
- Abstract:
- ... Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers. Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain d ...
- DOI:
- 10.1016/j.lfs.2018.02.033
- https://doi.org/10.1016/j.lfs.2018.02.033
- Author:
- Yu Zhang; Qiang Zhang; Hui Chen; Changli Wang
- Source:
- Life sciences 2018 v.208 pp. 284-294
- ISSN:
- 0024-3205
- Subject:
- Western blotting; agonists; beta catenin; cell lines; cisplatin; drug therapy; epithelium; fluorescent antibody technique; lung neoplasms; lymphoma; neoplasm cells; signal transduction
- Abstract:
- ... Bcell lymphoma 9 (BCL9)-Wnt/β-catenin pathway, a key signaling pathway related to epithelial-mesenchymal transition (EMT), is widely considered to be involved in invasion in various malignant tumors. However, the dysregulation of BCL9/β-catenin pathway in non-small cell lung cancer (NSCLC) has not been revealed. This study aimed to investigate the correlation between chemotherapy resistance and BC ...
- DOI:
- 10.1016/j.lfs.2018.07.023
- https://doi.org/10.1016/j.lfs.2018.07.023
- Author:
- Ruizan Shi; Diying Zhu; Zehui Wei; Naijie Fu; Chang Wang; Linhong Liu; Huifeng Zhang; Yueqin Liang; Jianfeng Xing; Xuening Wang; Yan Wang
- Source:
- Life sciences 2018 v.207 pp. 442-450
- ISSN:
- 0024-3205
- Subject:
- acetylcholine; bone morphogenetic proteins; cadherins; endothelial cells; fibrosis; hemodynamics; hypertension; hypertrophy; immunohistochemistry; males; polycyclic aromatic hydrocarbons; pulmonary artery; rats; staining; subcutaneous injection; transcription factor NF-kappa B; vimentin
- Abstract:
- ... Endothelial-to-mesenchymal transition (EndoMT) was shown to lead to endothelial cell (EC) dysfunction in pulmonary arterial hypertension (PAH). Baicalein was reported to inhibit epithelial-to-mesenchymal transition (EMT), a biological process that has many regulatory pathways in common with EndoMT. Whether it can attenuate PAH by inhibiting EndoMT remains obscure.PAH was induced by a single subcut ...
- DOI:
- 10.1016/j.lfs.2018.06.033
- https://doi.org/10.1016/j.lfs.2018.06.033
- Author:
- Junli Zhang; Haiming Zhang; Xiaoling Deng; Ning Zhang; Beibei Liu; Shengliang Xin; Guixin Li; Keshu Xu
- Source:
- Life sciences 2018 v.192 pp. 46-54
- ISSN:
- 0024-3205
- Subject:
- Scutellaria baicalensis; Western blotting; antioxidants; apoptosis; baicalin; blood serum; caspase-3; diet; enzyme-linked immunosorbent assay; fatty liver; fibrosis; hepatoprotective effect; inflammation; interleukin-1beta; lipid metabolism; lipids; liver; liver cirrhosis; macrophages; methionine; mice; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; transcription factor NF-kappa B; transforming growth factor beta 1; tumor necrosis factor-alpha
- Abstract:
- ... Baicalin (BA), an active flavonoid compound originating from the herb of Scutellaria baicalensis Georgi, has been previously shown to exert anti-inflammation and anti-oxidant effects in liver diseases. However, the potential role of BA in the regulation of non-alcoholic steatohepatitis (NASH) remains elusive. In this study, we newly explored the hepatoprotective effects of BA in MCD diet-induced N ...
- DOI:
- 10.1016/j.lfs.2017.11.027
- https://doi.org/10.1016/j.lfs.2017.11.027
- Author:
- Majel Cervantes-Llanos; Nielsen Lagumersindez-Denis; Javier Marín-Prida; Nancy Pavón-Fuentes; Viviana Falcon-Cama; Beatriz Piniella-Matamoros; Hanlet Camacho-Rodríguez; Julio Raúl Fernández-Massó; Carmen Valenzuela-Silva; Ivette Raíces-Cruz; Eduardo Pentón-Arias; Mauro Martins Teixeira; Giselle Pentón-Rol
- Source:
- Life sciences 2018 v.194 pp. 130-138
- ISSN:
- 0024-3205
- Subject:
- blood serum; interferon-gamma; brain; interleukin-6; signs and symptoms (animals and humans); enzyme-linked immunosorbent assay; mice; sclerosis; animal models; encephalitis; Arthrospira platensis; patients; quality of life; transmission electron microscopy; oral administration; spinal cord; oxidative stress; rats
- Abstract:
- ... The only three oral treatments currently available for multiple sclerosis (MS) target the relapsing forms of the disease and concerns regarding efficacy, safety and tolerability limit their use. Identifying novel oral disease-modifying therapies for MS, targeting both its inflammatory and neurodegenerative components is still a major goal.The scope of this study was to provide evidence that the or ...
- DOI:
- 10.1016/j.lfs.2017.12.032
- https://doi.org/10.1016/j.lfs.2017.12.032
- Author:
- Seidy Z. Castro-García; Germán Chamorro-Cevallos; Lucía Quevedo-Corona; Mark F. McCarty; Rosa A. Bobadilla-Lugo
- Source:
- Life sciences 2018 v.211 pp. 17-24
- ISSN:
- 0024-3205
- Subject:
- Spirulina maxima; antioxidant activity; females; free radicals; glucose; ischemia; laboratory animals; models; oral administration; pathophysiology; phycobiliprotein; pre-eclampsia; rats; sodium chloride; superoxide dismutase; systolic blood pressure; weight gain
- Abstract:
- ... Considering phycobiliproteins of Spirulina maxima has shown a wide margin of security in pregnant and non-pregnant animals as well as antioxidant properties, present study aimed to investigate if the cardiovascular and metabolic effects of an experimental model of preeclampsia can be prevented by the administration of this compound.Subrenal aortic coarctation (SRAC) practiced to female Wistar rats ...
- DOI:
- 10.1016/j.lfs.2018.09.011
- https://doi.org/10.1016/j.lfs.2018.09.011
- Author:
- Azza A.A. Galal; Amany Abdel-Rahman Mohamed; Safaa I. Khater; Mohamed M.M. Metwally
- Source:
- Life sciences 2018 v.201 pp. 9-16
- ISSN:
- 0024-3205
- Subject:
- GATA transcription factors; adults; biochanin A; blood serum; creatinine; dimethyl sulfoxide; estrogens; females; histology; inducible nitric oxide synthase; interleukin-4; ovariectomy; postmenopause; rats; tissues; transforming growth factor beta; tumor necrosis factor-alpha; urea; uric acid; women
- Abstract:
- ... This study was designed to assess the beneficial role of biochanin A (BCA) in the protection of the cutaneous and renal tissues of ovariectomized rats, even in those treated with anastrozole (ANA).For this purpose, 60 adult female Wistar rats were allocated into 6 equal groups. Rats in group I (sham) underwent a sham operation and received distilled water orally. In groups II and III, OVX rats rec ...
- DOI:
- 10.1016/j.lfs.2018.03.037
- https://doi.org/10.1016/j.lfs.2018.03.037
- Author:
- Pranesh Kumar; Archana S. Bhadauria; Ashok K. Singh; Sudipta Saha
- Source:
- Life sciences 2018 v.209 pp. 24-33
- ISSN:
- 0024-3205
- Subject:
- apoptosis; betulinic acid; clinical trials; cytotoxicity; drug therapy; mathematical models; mitochondria; neoplasm cells; skin neoplasms; tissues
- Abstract:
- ... A natural product betulinic acid (BA) has gained a huge significance in the recent years for its strong cytotoxicity. Surprisingly, in spite of being an interesting cancer protecting agent on a variety of tumor cells, the normal cells and tissues are rarely affected by BA. Betulinic acid and analogues (BAs) generally exert through the mechanisms that provokes an event of direct cell death and bypa ...
- DOI:
- 10.1016/j.lfs.2018.07.056
- https://doi.org/10.1016/j.lfs.2018.07.056
- Author:
- Zar Chi Thent; Gabriele Ruth Anisah Froemming; Suhaila Muid
- Source:
- Life sciences 2018 v.198 pp. 1-7
- ISSN:
- 0024-3205
- Subject:
- alkaline phosphatase; apoptosis; bisphenol A; bone resorption; calcitonin; calcium; endocrine-disrupting chemicals; environmental exposure; humans; osteoblasts; osteoclasts; propane; secretion; signal transduction; toxicity
- Abstract:
- ... Bisphenol A (BPA) (2,2,-bis (hydroxyphenyl) propane), a well-known endocrine disruptor (ED), is the exogenous chemical that mimic the natural endogenous hormone like oestrogen. Due to its extensive exposure to humans, BPA is considered to be a major toxicological agent for general population. Environmental exposure of BPA results in adverse health outcomes including bone loss. BPA disturbs the bon ...
- DOI:
- 10.1016/j.lfs.2018.02.013
- https://doi.org/10.1016/j.lfs.2018.02.013
- Author:
- Wei Qu; Zhenguo Zhao; Sijin Chen; Lijin Zhang; Danlian Wu; Zhigao Chen
- Source:
- Life sciences 2018 v.208 pp. 167-174
- ISSN:
- 0024-3205
- Subject:
- apoptosis; bisphenol A; cell proliferation; colorectal neoplasms; epithelial cells; epithelium; gene expression; gene expression regulation; humans; hydrogen peroxide; malondialdehyde; membrane potential; mitochondria; mitochondrial membrane; mitogen-activated protein kinase; models; neoplasm cells; pathogens; signal transduction; toxicity; viability
- Abstract:
- ... Bisphenol A (BPA) can act as pathogenic agent on mammalian cells. However, whether BPA induces colonic epithelial cell damage remains unexplored. We used HCT116 cells as a model to investigate the negative effect of BPA on human colon cancer cells and explore the potential mechanism. Our results suggest that BPA decreased viability of HCT116 cells. BPA also caused serious oxidative damage to the c ...
- DOI:
- 10.1016/j.lfs.2018.07.040
- https://doi.org/10.1016/j.lfs.2018.07.040
- Author:
- Soheila Rahmani; Nazila Pour Khalili; Fazlullah Khan; Shokoufeh Hassani; Elmira Ghafour-Boroujerdi; Mohammad Abdollahi
- Source:
- Life sciences 2018 v.214 pp. 136-144
- ISSN:
- 0024-3205
- Subject:
- DNA methylation; adipocytes; animals; bisphenol A; demethylation; endocrine-disrupting chemicals; epigenetics; estrogenic properties; fatty liver; genes; glucose; histones; homeostasis; insulin; islets of Langerhans; lipids; liver; microRNA; noninsulin-dependent diabetes mellitus; obesity; pollution; skeletal muscle; toxicity
- Abstract:
- ... Nowadays, endocrine disrupting chemical pollution has become one of the major concerns due to the potential role of these chemicals in provoking endocrine disorders especially type 2 diabetes. As a widespread endocrine disrupting chemical, Bisphenol A, with modest estrogenic activity can exert its detrimental effects in the different organs involved in type 2 diabetes such as pancreas, liver, adip ...
- DOI:
- 10.1016/j.lfs.2018.10.044
- https://doi.org/10.1016/j.lfs.2018.10.044
- Author:
- Helga Caputo Nunes; Wellerson Rodrigo Scarano; Elenice Deffune; Sérgio Luis Felisbino; Immacolata Porreca; Flávia Karina Delella
- Source:
- Life sciences 2018 v.206 pp. 22-28
- ISSN:
- 0024-3205
- Subject:
- adipogenesis; adipose tissue; adults; allografting; bisphenol A; clinical trials; endocrine-disrupting chemicals; homeostasis; humans; medicine; mesenchymal stromal cells; obesity; stem cells; surveys
- Abstract:
- ... Mesenchymal stem cells (MSCs) are found in all adult mesenchymal tissues. They play a role in the maintenance of tissue homeostasis and repair by allowing renewal of the cellular stock. MSCs can be isolated from both human and animal sources. These cells are important in regenerative medicine and cell therapy, thus adipose tissue is a rich and promising source of these cells. Adipose-derived stem ...
- DOI:
- 10.1016/j.lfs.2018.05.023
- https://doi.org/10.1016/j.lfs.2018.05.023
- Author:
- Everton Domingos; Marcos D. Polito
- Source:
- Life sciences 2018 v.209 pp. 122-131
- ISSN:
- 0024-3205
- Subject:
- blood flow; blood pressure; databases; guidelines; hypotension; meta-analysis; strength training; systematic review
- Abstract:
- ... The aim of this study was to compare, by means of a systematic review and meta-analysis, the effects of resistance training with and without blood flow restriction (BFR) on blood pressure (BP).This review was composed according to the preferred Reporting items for Systematic Reviews and Meta-Analyses guidelines. Searches were carried out in the databases PubMed, SPORTDiscus, and Web of Science. BP ...
- DOI:
- 10.1016/j.lfs.2018.08.006
- https://doi.org/10.1016/j.lfs.2018.08.006
- Author:
- Amany Abdel-Rahman Mohamed; Mona M. Ahmed; Mohamed Gomaa; Lamiaa L.M. Ebraheim
- Source:
- Life sciences 2018 v.212 pp. 159-167
- ISSN:
- 0024-3205
- Subject:
- X-radiation; adjuvants; animal models; biochanin A; biomarkers; blood serum; bone health; bone resorption; breast neoplasms; calcium; dimethyl sulfoxide; females; femur; histology; mineral content; morphometry; osteoporosis; ovariectomy; postmenopause; rats; risk; thermogravimetry; tibia; tumor necrosis factor-alpha; vertebrae; women
- Abstract:
- ... We investigate the consequence of adjuvant anastrozole (ANA) in monotherapy or associated with biochanin A (BCA) in ovariectomized (OVX) rat model and the degree of developing bone loss in both conditions.Sixty female rats were assigned to six groups. Five groups were bilaterally OVX, and one was sham operated. The five groups were; ANA group (0.5 mg/kg b.wt orally), BCA (5 mg/kg b.wt intraperiton ...
- DOI:
- 10.1016/j.lfs.2018.09.059
- https://doi.org/10.1016/j.lfs.2018.09.059
74. Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro
- Author:
- Lei Zhu; Juan Ma; Rui Mu; Ruiqiao Zhu; Feng Chen; Xiaocui Wei; Xiaolei Shi; Shengqi Zang; Lei Jin
- Source:
- Life sciences 2018 v.202 pp. 175-181
- ISSN:
- 0024-3205
- Subject:
- Western blotting; alizarin; bone morphogenetic proteins; cell proliferation; cell viability; dose response; immunocytochemistry; mineralization; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; staining; stem cells; tooth pulp; transcription (genetics)
- Abstract:
- ... in vitro effects of bone morphogenetic protein 7 (BMP-7) on proliferation and differentiation of dental pulp stem cells (DPSCs) have not been investigated, nor has an appropriate dose been established.Human DPSCs obtained from healthy volunteers were cultured with BMP-7 at 25, 50, and 100 ng/ml. Cell viability was measured by Cell Counting Kit-8 assay. Expression profiles of selected odontogenic d ...
- DOI:
- 10.1016/j.lfs.2018.03.026
- https://doi.org/10.1016/j.lfs.2018.03.026
- Author:
- Amadeu Z. Silva; Felipe P.L. Costa; Ingrid L. Souza; Mariana C. Ribeiro; Morenna Alana Giordani; Diogo A. Queiroz; Renata A.M. Luvizotto; André F. Nascimento; Gisele F. Bomfim; Mario M. Sugizaki; Robert F.H. Dekker; Aneli M. Barbosa-Dekker; Eveline A.I.F. Queiroz
- Source:
- Life sciences 2018 v.211 pp. 147-156
- ISSN:
- 0024-3205
- Subject:
- AMP-activated protein kinase; adipose tissue; adults; beta-glucans; cholesterol; comorbidity; diabetes; fatty liver; feed intake; glucose; glucose tolerance; glycogen; high density lipoprotein; high fat diet; hyperlipidemia; ingestion; insulin resistance; laboratory animals; lipid composition; liver; males; obesity; protein synthesis; rats; sucrose; transcription factors; triacylglycerols; very low density lipoprotein; weight gain
- Abstract:
- ... Obesity is associated with comorbidities such as diabetes and hepatic steatosis. β-Glucans have been described as effective in treating conditions including dyslipidaemia and diabetes. Thus, the objective of this study was to evaluate the effects of botryosphaeran [(1 → 3)(1 → 6)-β-D-glucan] on obesity and its comorbidities, and understand its mechanism of action.Obesity was induced in adult male ...
- DOI:
- 10.1016/j.lfs.2018.09.027
- https://doi.org/10.1016/j.lfs.2018.09.027
- Author:
- Cho-Rong Bae; Jun Hino; Hiroshi Hosoda; Mikiya Miyazato; Kenji Kangawa
- Source:
- Life sciences 2018 v.209 pp. 349-356
- ISSN:
- 0024-3205
- Subject:
- allidochlor; anti-inflammatory activity; asparagine; blood glucose; cholesterol; endothelial cells; fatty liver; fibrosis; free fatty acids; gene overexpression; genetically modified organisms; glucose tolerance; glucose tolerance tests; glycemic control; high fat diet; histology; hyperlipidemia; inflammation; insulin resistance; insulin tolerance test; lipid composition; liver; liver cirrhosis; mice; natriuretic peptides; obesity; risk; triacylglycerols
- Abstract:
- ... Our previous study revealed that mice transgenic for endothelial-cell–specific overexpression of CNP (E-CNP Tg mice) are protected against the increased fat weight, inflammation, and insulin resistance associated with high-fat diet (HFD)-induced obesity. In addition, E-CNP overexpression prevented abnormal lipid profiles and metabolism and blocked inflammation in the livers of HFD-fed mice. Becaus ...
- DOI:
- 10.1016/j.lfs.2018.08.031
- https://doi.org/10.1016/j.lfs.2018.08.031
- Author:
- Hongrui Chen; Lu Gao; Zhen Huang; Yuan Liu; Sen Guo; Junhui Xing; Zhe Meng; Cui Liang; Yapeng Li; Rui Yao; Ling Li; Yanzhou Zhang; Heping Gu; Yuzhou Liu
- Source:
- Life sciences 2018 v.207 pp. 492-498
- ISSN:
- 0024-3205
- Subject:
- apoptosis; cardiac output; cardiomyocytes; cell viability; doxorubicin; gene transfer; intraperitoneal injection; lactate dehydrogenase; mice; non-specific serine/threonine protein kinase; phosphorylation; protein content; therapeutics; toxicity; troponin I
- Abstract:
- ... The clinical use of doxorubicin (Dox) is limited due to a degenerative irreversible cardiac toxicity, but the precise mechanisms that contribute to this pathological response are not understood. C1q/TNF-related protein 1 (CTRP1), which is a conserved protein of the C1q family, has notable metabolic and cardiovascular functions. However, whether CTRP1 can attenuate Dox-induced cardiac injury remain ...
- DOI:
- 10.1016/j.lfs.2018.06.029
- https://doi.org/10.1016/j.lfs.2018.06.029
- Author:
- Georg Daniel Duerr; Andreas Feißt; Katharina Halbach; Luise Verfuerth; Christopher Gestrich; Daniela Wenzel; Andreas Zimmer; Johannes Breuer; Oliver Dewald
- Source:
- Life sciences 2018 v.215 pp. 96-105
- ISSN:
- 0024-3205
- Subject:
- animal models; apoptosis; cannabinoids; cardiomyocytes; collagen; enzymes; hemodynamics; hypertension; hypertrophy; immunohistochemistry; inflammation; interleukin-10; ischemia; long term effects; lungs; macrophages; messenger RNA; mice; myosin; organ transplantation; protective effect; pulmonary artery
- Abstract:
- ... Pulmonary hypertension (PH) leads to right ventricular (RV) adaptation and remodeling and has deleterious long-term effects on RV function. The endocannabinoid receptor CB2 has been associated with protective effects in adaptation and remodeling of the left ventricle after ischemia. Therefore, we investigated the role of CB2 receptor in RV adaptation after occlusion of the left pulmonary artery (L ...
- DOI:
- 10.1016/j.lfs.2018.11.003
- https://doi.org/10.1016/j.lfs.2018.11.003
- Author:
- Amr Ahmed El-Arabey; Salama Abdu Salama; Adel Rashad Abd-Allah
- Source:
- Life sciences 2018 v.208 pp. 192-200
- ISSN:
- 0024-3205
- Subject:
- cell growth; chromosomes; drug therapy; genomics; mitosis; mitotic spindle apparatus; neoplasm cells; neoplasms; neurons; paclitaxel; plasticity
- Abstract:
- ... In 2015, more than 1.6 million new cancer cases with 589,430 deaths were estimated over worldwide. Cancer is a complex disease with abnormal cell growth control which is hallmarked by chromosome misalignment and consequently genomic instability. Mitosis is a well-known target for chemotherapy as taxol and colchicines inhibit tumor cell division by inhibiting mitotic spindle plasticity. Accumulatin ...
- DOI:
- 10.1016/j.lfs.2018.07.037
- https://doi.org/10.1016/j.lfs.2018.07.037
- Author:
- Maria Cristina Aisa; Alessandro Datti; Antonio Orlacchio; Gian Carlo Di Renzo
- Source:
- Life sciences 2018 v.208 pp. 10-19
- ISSN:
- 0024-3205
- Subject:
- analgesia; aspirin; cathepsin B; cell proliferation; comorbidity; enzyme activity; inflammation; metabolism; metalloproteinases; nitroprusside; nonsteroidal anti-inflammatory agents; osteoblasts; osteoporosis; pain; plasminogen activator; postmenopause; women
- Abstract:
- ... Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for the treatment of pain and inflammation. Although it is well known that NSAIDs can suppress bone growth, remodelling and repair, they are largely used post-operatively and post-traumatically to achieve analgesia and reduce inflammation in bone tissue.The impact of two NO-releasing, non-selective NSAIDs, NCX-4016 and HCT-3012 ...
- DOI:
- 10.1016/j.lfs.2018.07.011
- https://doi.org/10.1016/j.lfs.2018.07.011
- Author:
- Lan-Zhi Zhang; Li-Yan Huang; An-Liang Huang; Jin-Xing Liu; Fan Yang
- Source:
- Life sciences 2018 v.207 pp. 420-427
- ISSN:
- 0024-3205
- Subject:
- Western blotting; beta catenin; cell lines; cell movement; humans; immunohistochemistry; metastasis; mortality; neoplasm cells; protein synthesis; quantitative polymerase chain reaction; signal transduction; small interfering RNA; tissues; transfection; uterine cervical neoplasms; women
- Abstract:
- ... Cervical cancer (CC) is the third most common cancer and the fourth leading cause of malignancy-related mortality in women worldwide. In addition, epithelial–mesenchymal transition (EMT) has been generally studied in tumor metastasis researches in recent years. Cysteine-rich intestinal protein 1 (CRIP1) is differently expressed in human cancer cells. However, the role it plays in CC has not been r ...
- DOI:
- 10.1016/j.lfs.2018.05.054
- https://doi.org/10.1016/j.lfs.2018.05.054
82. CRTC2 suppresses BMP2-induced osteoblastic differentiation via Smurf1 expression in MC3T3-E1 cells
- Author:
- Kyeong-Min Kim; Wan-Jin Jeon; Eun-Jung Kim; Won-Gu Jang
- Source:
- Life sciences 2018 v.214 pp. 70-76
- ISSN:
- 0024-3205
- Subject:
- Western blotting; alizarin; alkaline phosphatase; bone formation; cyclic AMP; extracellular matrix; gene overexpression; genes; gluconeogenesis; luciferase; messenger RNA; mineralization; mothers against decapentaplegic homolog proteins; osteoblasts; protein content; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; transcription (genetics); tumor necrosis factor-alpha
- Abstract:
- ... CREB (cAMP response element-binding protein)-regulated transcription coactivator (CRTC2) has been reported to act as a coactivator of CREB during gluconeogenesis. The role of CRTC2 in osteoblastic differentiation has not yet been elucidated. The aim of this study is to identify the mechanism of CRTC2 in osteoblast differentiation.The mRNA expression was determined by RT-PCR and qPCR. Protein level ...
- DOI:
- 10.1016/j.lfs.2018.10.052
- https://doi.org/10.1016/j.lfs.2018.10.052
- Author:
- Chao Xu; Linfeng Zheng; Dechuan Li; Guoping Chen; Jianzhong Gu; Jun Chen; Qinghua Yao
- Source:
- Life sciences 2018 v.208 pp. 333-340
- ISSN:
- 0024-3205
- Subject:
- CXCR4 receptor; DNA repair; base pair mismatch; biomarkers; colorectal neoplasms; confidence interval; human health; immunohistochemistry; lymph nodes; metastasis; mortality; multivariate analysis; prediction; prognosis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; tissues
- Abstract:
- ... Colorectal cancer threatens human health due to its high mortality resulting from metastatic progression. The expression of C-X-C chemokine receptor type 4 (CXCR4) is absent or low in most healthy tissues but high in various types of tumours. In this study, we aim to determine the prognostic significance of CXCR4 in colorectal cancer.We retrospectively examined a total of 72 tissue samples, that q ...
- DOI:
- 10.1016/j.lfs.2018.04.050
- https://doi.org/10.1016/j.lfs.2018.04.050
- Author:
- Bruna Romana-Souza; Jeanine Salles dos Santos; Andréa Monte-Alto-Costa
- Source:
- Life sciences 2018 v.207 pp. 158-165
- ISSN:
- 0024-3205
- Subject:
- animal models; caffeic acid; collagen; inducible nitric oxide synthase; inflammation; ischemia; lipid peroxidation; macrophages; magnetism; mice; nitric oxide; pressure ulcers; propolis; protein synthesis; tissue repair; transcription factor NF-kappa B
- Abstract:
- ... In pressure ulcers, the synthesis of reactive oxygen species induced by ischemia and reperfusion leads to chronic inflammation and tissue damage, which impair the closure of these lesions. Caffeic acid phenethyl ester (CAPE), found in propolis, promotes cutaneous wound healing of acute lesions and severe burns. However, the effects of CAPE on wound healing of pressure ulcers have not been investig ...
- DOI:
- 10.1016/j.lfs.2018.05.057
- https://doi.org/10.1016/j.lfs.2018.05.057
- Author:
- Xiangrong Sun; Lieqi Tang; Steven Winesett; Wenhan Chang; Sam Xianjun Cheng
- Source:
- Life sciences 2018 v.194 pp. 49-58
- ISSN:
- 0024-3205
- Subject:
- agonists; calcium receptors; chlorides; colon; electrolytes; mice; muscles; mutants; neurons; protein synthesis; rats; secretion; tetrodotoxin
- Abstract:
- ... Calcium-sensing receptor (CaSR) is expressed on neurons of both submucosal and myenteric plexuses of the enteric nervous system (ENS) and the CaSR agonist R568 inhibited Cl⁻ secretion in intestine. The purpose of this study was to localize the primary site of action of R568 in the ENS and to explore how CaSR regulates secretion through the ENS.Two preparations of rat proximal and distal colon were ...
- DOI:
- 10.1016/j.lfs.2017.12.019
- PubMed:
- 29247746
- PubMed Central:
- PMC5794538
- https://doi.org/10.1016/j.lfs.2017.12.019
- Author:
- Ewa Poleszak; Sylwia Wośko; Karolina Sławińska; Aleksandra Szopa; Andrzej Wróbel; Anna Serefko
- Source:
- Life sciences 2018 v.213 pp. 18-24
- ISSN:
- 0024-3205
- Subject:
- Alzheimer disease; Cannabis sativa; Parkinson disease; agonists; antagonists; antidepressants; cannabinoids; epilepsy; medicinal plants; mental depression; neuroprotective effect; pathogenesis; patients; receptors; sclerosis; therapeutics
- Abstract:
- ... Cannabis sativa is one of the most popular recreational and medicinal plants. Benefits from use of cannabinoid agents in epilepsy, multiple sclerosis, Parkinson's disease, Alzheimer's disease, and others have been suggested. It seems that the endocannabinoid system is also involved in the pathogenesis and treatment of depression, though its role in this mental disease has not been fully understood ...
- DOI:
- 10.1016/j.lfs.2018.09.058
- https://doi.org/10.1016/j.lfs.2018.09.058
- Author:
- Zhengtu Li; Junling Zhao; Hongxia Zhou; Li Li; Yuyang Ding; Jing Li; Beixian Zhou; Haiming Jiang; Nanshan Zhong; Wenhui Hu; Zifeng Yang
- Source:
- Life sciences 2018 v.200 pp. 115-125
- ISSN:
- 0024-3205
- Subject:
- Influenza A virus; animal models; anti-inflammatory activity; chemokines; cytopathogenicity; enzyme-linked immunosorbent assay; humans; inflammation; influenza; interferon-beta; interleukin-6; lipopolysaccharides; mice; progeny; quantitative polymerase chain reaction; signal transduction; virus replication; viruses
- Abstract:
- ... This study aimed to evaluate the efficacy and mechanisms of Cappariloside A, a chemically synthesized compound, against virus and inflammation induced by influenza virus.The inhibitory activity of Cappariloside A against influenza virus was determined by plaque assay and cytopathic effect inhibition assay. Quantitative real-time PCR, enzyme-linked immunosorbent assay and Bio-Plex methods were used ...
- DOI:
- 10.1016/j.lfs.2018.03.033
- https://doi.org/10.1016/j.lfs.2018.03.033
- Author:
- F. Mayyas; K.H. Alzoubi
- Source:
- Life sciences 2018 v.211 pp. 279-285
- ISSN:
- 0024-3205
- Subject:
- C-reactive protein; air; animal models; biomarkers; body weight; cardiovascular diseases; catalase; cigarettes; citrates; diabetes; enzyme activity; fibrosis; gelatinase A; heart; inflammation; interleukin-6; intraperitoneal injection; laboratory animals; myeloperoxidase; nitrites; oxidants; oxidative stress; rats; risk; smoking (habit); streptozotocin; superoxide dismutase; systolic blood pressure; thiobarbituric acid; transforming growth factor beta
- Abstract:
- ... Tobacco smoking is considered a global health issue, contributing to increased risk of cardiovascular disease (CVD) and diabetes (DM). We aimed to assess effects of cigarette smoking on cardiac inflammation, oxidative stress and fibrosis in rat model of streptozotocin (STZ)-induced diabetes.Adults Wistar rats were assigned into control (fresh air, intraperitoneal injection (i.p) of citrate buffer) ...
- DOI:
- 10.1016/j.lfs.2018.09.038
- https://doi.org/10.1016/j.lfs.2018.09.038
- Author:
- Neema Kumari; Himanshu Gaur; Anamika Bhargava
- Source:
- Life sciences 2018 v.194 pp. 139-149
- ISSN:
- 0024-3205
- Subject:
- beta adrenergic receptors; calcium; calcium channels; heart; heart failure
- Abstract:
- ... Voltage-gated calcium channels (VGCCs) are the predominant source of calcium influx in the heart leading to calcium-induced calcium release and ultimately excitation-contraction coupling. In the heart, VGCCs are modulated by the β-adrenergic signaling. Signaling through β-adrenergic receptors (βARs) and modulation of VGCCs by β-adrenergic signaling in the heart are critical signaling and changes t ...
- DOI:
- 10.1016/j.lfs.2017.12.033
- https://doi.org/10.1016/j.lfs.2017.12.033
- Author:
- Ayesheh Enayati; Narguess Yassa; Zohreh Mazaheri; Maryam Rajaei; Mona Pourabouk; Sajjad Ghorghanlu; Salar Basiri; Vahid Khori
- Source:
- Life sciences 2018 v.215 pp. 216-226
- ISSN:
- 0024-3205
- Subject:
- Potentilla reptans; antioxidant activity; antioxidants; apoptosis; arrhythmia; cardioprotective effect; caspase-3; catalase; ethyl acetate; heart; hemodynamics; immunohistochemistry; infarction; ischemia; laboratory animals; models; nitric oxide; proanthocyanidins; rats; staining; superoxide dismutase
- Abstract:
- ... Previous studies have shown that proanthocyanidins have cardioprotective effects which are mediated via the release of nitric oxide (NO) ultimately resulting in increasing the antioxidant activity. We have investigated to show whether 1) the total extract and ethyl acetate fraction (Et) of Potentilla reptans root have an ischemic preconditioning (IPC) effect, 2) P. reptans has antioxidant and card ...
- DOI:
- 10.1016/j.lfs.2018.11.021
- https://doi.org/10.1016/j.lfs.2018.11.021
- Author:
- Seham Zakaria Nassar; Passainte S. Hassaan; Doaa A. Abdelmonsif; Samar Nabil ElAchy
- Source:
- Life sciences 2018 v.201 pp. 89-101
- ISSN:
- 0024-3205
- Subject:
- spironolactone; blood serum; lung function; oxidative stress; ceric oxide; cardioprotective effect; hypertension; monocrotaline; albino; subcutaneous injection; animal models; rats; nanoparticles; antioxidant activity; antioxidants; endothelins; hypertrophy
- Abstract:
- ... Cerium oxide nanoparticles (CeO2NPs) have been recently introduced into the medical field for their antioxidant properties. The ability of CeO2NPs alone or in combination with spironolactone (SP) to attenuate monocrotaline (MCT)-induced pulmonary hypertension and associated right ventricular hypertrophy was studied in rats. A special emphasis was given to endothelin-1 pathway.Pulmonary hypertensio ...
- DOI:
- 10.1016/j.lfs.2018.03.045
- https://doi.org/10.1016/j.lfs.2018.03.045
- Author:
- Navideh Mirzakhani; Amir Abbas Farshid; Esmaeal Tamaddonfard; Mehdi Imani; Amir Erfanparast; Farahnaz Noroozinia
- Source:
- Life sciences 2018 v.215 pp. 22-30
- ISSN:
- 0024-3205
- Subject:
- antioxidant activity; axons; carnosine; electron microscopes; histology; histopathology; malondialdehyde; muscles; muscular atrophy; myelin sheath; myelination; nerve regeneration; nerve tissue; normal values; oxidative stress; rats; superoxide dismutase; therapeutics; tumor necrosis factor-alpha
- Abstract:
- ... Peripheral nerve injury represents a substantial clinical problem with insufficient or unsatisfactory treatment options. Current researches have extensively focused on the new approaches for the treatment of peripheral nerve injuries. Carnosine is a naturally occurring pleotropic dipeptide and has many biological functions such as antioxidant property. In the present study, we examined the regener ...
- DOI:
- 10.1016/j.lfs.2018.10.043
- https://doi.org/10.1016/j.lfs.2018.10.043
- Author:
- Esteban Alberto Gonzalez; Giselle Renata Martins; Angela Maria Vicente Tavares; Michelle Viegas; Edina Poletto; Roberto Giugliani; Ursula Matte; Guilherme Baldo
- Source:
- Life sciences 2018 v.196 pp. 102-109
- ISSN:
- 0024-3205
- Subject:
- animal models; aorta; cardiac output; cathepsin B; elastase; enzyme activity; extracellular matrix; fibroblasts; heart valves; humans; lysosomes; mice; mucopolysaccharidosis; myocardium
- Abstract:
- ... Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disorder with multisystemic features, including heart enlargement, heart valve dysfunction, and aortic stiffness and dilatation. Previous studies have shown that MPS I mice overexpress cathepsin B (CtsB) in multiple tissues, including those from the cardiovascular system. Here, we hypothesized that inhibition of CtsB could ameliorate card ...
- DOI:
- 10.1016/j.lfs.2018.01.020
- https://doi.org/10.1016/j.lfs.2018.01.020
- Author:
- Jerald J. Nair; Johannes van Staden
- Source:
- Life sciences 2018 v.213 pp. 94-101
- ISSN:
- 0024-3205
- Subject:
- Amaryllidaceae; alkaloids; antineoplastic agents; apoptosis; cell cycle; cell lines; cytotoxicity; death; drug therapy; immunity; mechanism of action; neoplasm cells; neoplasms; structure-activity relationships
- Abstract:
- ... The birth, growth, proliferation and death of cells involve a rigorous and continuous process in place to ensure the survival of living organisms. The cell cycle prevails at the core of this process to facilitate the division of a parent cell as well as the duplication of its genetic matter. Although checkpoints exist to steer the course of a cell from one phase to the other, malfunctions at any p ...
- DOI:
- 10.1016/j.lfs.2018.08.073
- https://doi.org/10.1016/j.lfs.2018.08.073
95. Centchroman regulates breast cancer angiogenesis via inhibition of HIF-1α/VEGFR2 signalling axis
- Author:
- Jayant Dewangan; Shweta Kaushik; Srikanta Kumar Rath; Anil K. Balapure
- Source:
- Life sciences 2018 v.193 pp. 9-19
- ISSN:
- 0024-3205
- Subject:
- Western blotting; angiogenesis; apoptosis; breast neoplasms; breasts; calcium; cell proliferation; cell viability; chickens; chorioallantoic membrane; drugs; endothelial cells; epithelial cells; estrogen receptors; flow cytometry; humans; hypoxia-inducible factor 1; metastasis; migratory behavior; mitogen-activated protein kinase; neoplasm cells; phosphorylation; reactive oxygen species; vascular endothelial growth factor receptor-2; vascular endothelial growth factors
- Abstract:
- ... Angiogenesis is a recognized hallmark of cancer which promotes cancer cell progression and metastasis. Inhibition of angiogenesis to attenuate cancer growth is becoming desirable strategy for breast cancer management. The present study is aimed to investigate the antiangiogenic efficacy of a novel selective estrogen receptor modulator Centchroman (CC) on human breast cancer cells.Effect of CC on c ...
- DOI:
- 10.1016/j.lfs.2017.11.045
- https://doi.org/10.1016/j.lfs.2017.11.045
- Author:
- Mohammed Alotaibi
- Source:
- Life sciences 2018 v.199 pp. 151-157
- ISSN:
- 0024-3205
- Subject:
- adenosine triphosphate; calcium; enzyme-linked immunosorbent assay; myometrium; pregnancy; rats; receptors; uterine contraction
- Abstract:
- ... Given the importance of ATP in the control of uterine activity for successful labor and involution, this study was performed to measure the level of P2X7 receptors (P2X7Rs) in rat myometrium at different gestational stages and to investigate the mechanisms of ATP-induced uterine contraction. Myometrial tissues were obtained from rats at different gestational stages and the level of P2X7Rs was meas ...
- DOI:
- 10.1016/j.lfs.2018.03.016
- https://doi.org/10.1016/j.lfs.2018.03.016
- Author:
- Anna Lesniak; Mikko Aarnio; Shanti Diwakarla; Thomas Norberg; Fred Nyberg; Torsten Gordh
- Source:
- Life sciences 2018 v.194 pp. 26-33
- ISSN:
- 0024-3205
- Subject:
- amine oxidase (flavin-containing); binding capacity; binding sites; explants; humans; inflammation; osteoarthritis; patients; positron-emission tomography; synovitis; tritium
- Abstract:
- ... d-Deprenyl when used as a positron emission tomography tracer visualizes peripheral inflammation. The major aim of the current study was to identify and investigate the properties of the binding target for d-deprenyl in synovial membrane explants from arthritic patients.Thirty patients diagnosed with arthritis or osteoarthritis were enrolled into the study. Homologous and competitive radioligand b ...
- DOI:
- 10.1016/j.lfs.2017.12.003
- https://doi.org/10.1016/j.lfs.2017.12.003
- Author:
- Cui Zhang; Jun Lin; Fang Wei; Jian Song; Wenyue Chen; Lidong Shan; Rong Xue; Guoqing Wang; Jin Tao; Guoxing Zhang; Guang-Yin Xu; Linhui Wang
- Source:
- Life sciences 2018 v.201 pp. 150-160
- ISSN:
- 0024-3205
- Subject:
- acetazolamide; astrocytes; blood vessels; brain; cadaverine; cerebrospinal fluid; extracellular space; fluorescence; image analysis; mice; neurons; ovalbumin; parenchyma (animal tissue); tracer techniques
- Abstract:
- ... Accumulating evidence supports that cerebrospinal fluid (CSF) in the subarachnoid space (SAS) could reenter the brain parenchyma via the glymphatic influx. The present study was designed to characterize the detailed pathway of subarachnoid CSF influx by using a novel CSF tracer.Fluorescently conjugated cadaverine (A488-ca), for the first time, was employed to investigate CSF movement in the brain. ...
- DOI:
- 10.1016/j.lfs.2018.03.057
- https://doi.org/10.1016/j.lfs.2018.03.057
- Author:
- Pratibha Chauhan; Akhilesh Kumar Tamrakar; Sunil Mahajan; G.B.K.S. Prasad
- Source:
- Life sciences 2018 v.213 pp. 226-235
- ISSN:
- 0024-3205
- Subject:
- Fourier transform infrared spectroscopy; Western blotting; anti-inflammatory activity; chitosan; curcumin; differential scanning calorimetry; enzyme-linked immunosorbent assay; glycemic effect; hydrophilicity; myotubes; nanoparticles; particle size; phosphorylation; plasma membrane; skeletal muscle; solubility; therapeutics; transmission electron microscopy
- Abstract:
- ... The present study was undertaken to develop a Curcumin nanoparticle system with chitosan as a hydrophilic carrier. In addition, the anti-diabetic potential of curcumin loaded chitosan nanoparticles were assessed in comparison to those of free curcumin by examining the anti-hyperglycemic efficacy using in vitro assays.Curcumin loaded chitosan nanoparticles were prepared and characterized for partic ...
- DOI:
- 10.1016/j.lfs.2018.10.027
- https://doi.org/10.1016/j.lfs.2018.10.027
- Author:
- Tao Wu; Luna Yang; Jianguo Jiang; Yinhua Ni; Jiawei Zhu; Xiaojun Zheng; Qi Wang; Xin Lu; Zhengwei Fu
- Source:
- Life sciences 2018 v.192 pp. 173-182
- ISSN:
- 0024-3205
- Subject:
- adrenal glands; biogenesis; blood serum; body weight changes; circadian clocks; circadian rhythm; colon; corticosterone; dexamethasone; food intake; genes; high-throughput nucleotide sequencing; intestinal microorganisms; lipid metabolism; lipids; liver; messenger RNA; metabolic syndrome; mitochondria; mucus; oral administration; rats; ribosomal RNA; secretion; sodium phosphate; steroid hormones; tissues
- Abstract:
- ... Glucocorticoids (GCs), steroid hormones synthetized by the adrenal gland, are regulated by circadian cycles, and dysregulation of GC signaling can lead to the development of metabolic syndrome. The effects and potential mechanism of GCs in physiology were investigated in the present study.Male Wistar rats were orally administered dexamethasone sodium phosphate (DEX, 0.01 and 0.05mg/kg body weight ...
- DOI:
- 10.1016/j.lfs.2017.11.049
- https://doi.org/10.1016/j.lfs.2017.11.049