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- Kim, Kyeong-Min; Jeon, Wan-Jin; Kim, Eun-Jung; Jang, Won-Gu
- Life sciences 2018 v.214 pp. 70-76
- Western blotting; alizarin; alkaline phosphatase; bone formation; cyclic AMP; extracellular matrix; gene overexpression; genes; gluconeogenesis; luciferase; messenger RNA; mineralization; mothers against decapentaplegic homolog proteins; osteoblasts; protein content; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; transcription (genetics); tumor necrosis factor-alpha
- ... CREB (cAMP response element-binding protein)-regulated transcription coactivator (CRTC2) has been reported to act as a coactivator of CREB during gluconeogenesis. The role of CRTC2 in osteoblastic differentiation has not yet been elucidated. The aim of this study is to identify the mechanism of CRTC2 in osteoblast differentiation.The mRNA expression was determined by RT-PCR and qPCR. Protein level ...