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1. Cholesterol metabolism in copper deficient rats

Author:
Allen, Kenneth G.D.; Klevay, Leslie M.
Source:
Life sciences 1978 v.22 no.19 pp. 1691-1697
ISSN:
0024-3205
Subject:
blood lipids; cholesterol; physiological transport; nutrient deficiencies; dietary minerals; copper; mevalonic acid; lipid metabolism; liver; rats; animal disease models; human nutrition; human health
Abstract:
... The influence of Cu deficiency on the appearance of newly synthesized cholesterol in the plasma lipids of rats was examined following 3H mevalonate injection. At 181 days Cu deficient rats exhibited a highly significant increase in plasma cholesterol concentration. Cu deficiency was associated with a greatly enhanced appearance of 3H in newly synthesized cholesterol and cholesteryl esters in the p ...
Handle:
10113/45591
PubMed:
672420

2. In vitro hepatic thyroid hormone deiodination in iron-deficient rats: effect of dietary fat

Author:
Smith, Scott M.; Johnson, Phyllis E.; Lukaski, Henry C.
Source:
Life sciences 1993 v.53 no.8 pp. 603-609
Subject:
metabolism; liver; dietary fat; nutrient deficiencies; iron; stearic acid; triiodothyronine; fat intake; in vitro studies; rats; safflower oil; tallow
Abstract:
... The effects of different dietary fats on thyroid indices were studied in weanling iron-deficient rats. Rats were fed one of five different diets (safflower oil with a casein protein source, safflower oil with defatted beef as the protein source, prime rib, beef tallow with casein and stearate with casein). Both dietary fat and iron status (adequate, CN; deficient, ID; or iron-deficient replete, ID ...
Handle:
10113/44654
DOI:
10.1016/0024-3205(93)90268-8
PubMed:
8350675

3. The microphotohemolytic response of erythrocytes is altered by streptozotocin-induced diabetes and copper deficiency in rats

Author:
Miller, F.N.; Saari, J.T.; Alsip, N.L.; Schuschke, D.A.
Source:
Life sciences 1995 v.56 no.10 pp. 735-745
Subject:
erythrocytes; hemolysis; cell membranes; analytical methods; copper; nutrient deficiencies; diabetes; streptozotocin; hypercholesterolemia; rats
Abstract:
... Microphotohemolysis is a new technique that has been used to determine the presence of alterations in the erythrocyte membrane. The method involves light activation through a microscope of a fluorescent dye-erythrocyte-buffer solution in a hemocytometer. The interaction of the light and dye result in the generation of toxic oxygen products which attack the membrane allowing water to enter the cell ...
Handle:
10113/44553
DOI:
10.1016/0024-3205(95)00004-P

4. Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells

Author:
Carlson, S.A.; Omary, M.B.; Jones, B.D.
Source:
Life sciences 2002 v.70 no.12 pp. 1415-1426
Subject:
Salmonella; animal pathogenic bacteria; pathogenesis; eukaryotic cells; keratin; complementary DNA; bacterial proteins; host-pathogen relationships; protein-protein interactions; actin; nucleotide sequences; amino acid sequences
Abstract:
... Pathogenic Salmonella species initiate infection of a mammalian host by inducing their own uptake into intestinal M-cells. During the uptake process, the bacteria utilize an intrinsic secretion system to release proteins that enter host cells. The secreted invasion-mediating proteins subsequently interact with host cell components that induce alterations in the actin cytoskeleton. To identify pote ...
Handle:
10113/4049
DOI:
10.1016/S0024-3205(01)01512-0

5. Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus): Effect of seawater exposure and cortisol treatment

Author:
Takahashi, Hideya; Hyodo, Susumu; Shepherd, Brian S.; Kaneko, Toyoji; Grau, E. Gordon
Source:
Life sciences 2006 v.78 no.20 pp. 2329-2335
Subject:
Oreochromis mossambicus; tilapia (common name); freshwater fish; intestines; seawater; saline water; osmoregulation; cortisol; receptors; gene expression regulation
Abstract:
... Cortisol plays an important role in controlling intestinal water and ion transport in teleosts possibly through glucocorticoid receptor (GR) and/or mineralocorticoid receptor. To better understand the role of GR in the teleost intestine, in a euryhaline tilapia, Oreochromis mossambicus, we examined (1) the intestinal localizations of GR; (2) the effects of environmental salinity challenge and cort ...
Handle:
10113/36495
DOI:
10.1016/j.lfs.2005.09.050

6. (+)-Morphine attenuates the (−)-morphine-produced tail-flick inhibition via the sigma-1 receptor in the mouse spinal cord

Author:
Leon F. Tseng; Quinn H. Hogan; Hsiang-En Wu
Source:
Life sciences 2011 v.89 no.23-24 pp. 875-877
ISSN:
0024-3205
Subject:
analgesia; mice; receptors; spinal cord
Abstract:
... AIMS: We have previously demonstrated that pretreatment with (+)-morphine given intrathecally attenuates the intrathecal (−)-morphine-produced tail-flick inhibition. The phenomenon has been defined as antianalgesia against (−)-morphine-produced analgesia. Present experiments were then undertaken to determine if the antianalgesic effect induced by (+)-morphine given spinally is mediated by the stim ...
DOI:
10.1016/j.lfs.2011.09.018
PubMed:
21989208
PubMed Central:
PMC3220751

7. (−)-Epigallocatechin-3-gallate blocks 3T3-L1 adipose conversion by inhibition of cell proliferation and suppression of adipose phenotype expression

Author:
Cheuk Ying Chan; Lai Wei; Federico Castro-Muñozledo; Wing Leung Koo
Source:
Life sciences 2011 v.89 no.21-22 pp. 779-785
ISSN:
0024-3205
Subject:
adipogenesis; adipose tissue; binding proteins; bioactive properties; body fat; cell cycle; cell proliferation; droplets; flow cytometry; green tea; homeostasis; mice; personal development; phenotype; transcription factors
Abstract:
... AIMS: A major objective in the treatment of obese individuals is the development of agents that reduce body fat and improve metabolic homeostasis. Among a variety of biological effects, green tea exerts an anti-obesity effect; however, the mechanisms behind its activity on adipose tissue are uncertain. Tea contains high levels of (−)-epigallocatechin-3-gallate (EGCG), which is one of its main bioa ...
DOI:
10.1016/j.lfs.2011.09.006

8. 16-Hydroxycleroda-3,13-dien-15,16-olide regulates the expression of histone-modifying enzymes PRC2 complex and induces apoptosis in CML K562 cells

Author:
Yi-Hsiung Lin; Chien-Chih Lee; Fang-Rong Chang; Wen-Hsin Chang; Yang-Chang Wu; Jan-Gowth Chang
Source:
Life sciences 2011 v.89 no.23-24 pp. 886-895
ISSN:
0024-3205
Subject:
quantitative polymerase chain reaction; epigenetics; protein synthesis; cell cycle; drug therapy; leukemia; histones; enzymes; tumor suppressor genes; Western blotting; gene expression; gene silencing; cell proliferation; fluorescent antibody technique; apoptosis
Abstract:
... AIMS: Histone modifications play central epigenetic roles in regulating the entire genome of the cell and cell proliferation. Herein, we investigated the effects of the natural compound, 16-hydroxycleroda-3,13-dien-15,16-olide (PL3), on the expressions of histone-modifying enzymes, and examined how it induces apoptosis in leukemia K562 cells. MAIN METHODS: Cell proliferation was determined by an M ...
DOI:
10.1016/j.lfs.2011.09.011

9. 24, 25-Dihydroxycholecalciferol but not 25-hydroxycholecalciferol suppresses apolipoprotein A-I gene expression

Author:
Kent R. Wehmeier; Abdul-Razzak Alamir; Senan Sultan; Michael J. Haas; Norman C.W. Wong; Arshag D. Mooradian
Source:
Life sciences 2011 v.88 no.1-2 pp. 110-116
ISSN:
0024-3205
Subject:
25-hydroxycholecalciferol; Northern blotting; apolipoprotein A-I; gene expression; hepatocytes; human cell lines; intestines; messenger RNA; metabolites; plasmids; reporter genes; secretion
Abstract:
... AIMS: Ligands for the vitamin D receptor (VDR) regulate apolipoprotein A-I (apo A-I) gene expression in a tissue-specific manner. The vitamin D metabolite 24, 25-dihydroxycholecalciferol (24, 25-(OH)₂D₃) has been shown to possess unique biological effects. To determine if 24, 25-(OH)₂D₃ modulates apo A-I gene expression, HepG2 hepatocytes and Caco-2 intestinal cells were treated with 24, 25-(OH)₂D ...
DOI:
10.1016/j.lfs.2010.11.005

10. 8-Prenylkaempferol accelerates osteoblast maturation through bone morphogenetic protein-2/p38 pathway to activate Runx2 transcription

Author:
Wen-Fei Chiou; Chia-Hsin Lee; Jyh-Fei Liao; Chien-Chih Chen
Source:
Life sciences 2011 v.88 no.7-8 pp. 335-342
ISSN:
0024-3205
Subject:
Sophora flavescens; Western blotting; alkaline phosphatase; antagonists; bone formation; bone morphogenetic proteins; collagen; cytotoxicity; gene expression; gene expression regulation; messenger RNA; mice; mineralization; osteoblasts; osteocalcin; osteopontin; reverse transcriptase polymerase chain reaction; signal transduction; transcription factors
Abstract:
... AIMS: In this study, we investigated the effect of 8-prenylkaempferol (8-PK), a prenyl-flavonoid isolated from Sophora flavescens, on osteoblast differentiation and maturation. MAIN METHODS: MC3T3-E1 cells were exposed to 8-PK and the cytotoxicity was assayed. Osteoblast differentiation and maturation were evaluated by analyzing alkaline phosphatase (ALP) activity and cell mineralization, respecti ...
DOI:
10.1016/j.lfs.2010.12.009

11. A key role for KCl cotransport in cell volume regulation in human erythroleukemia cells

Author:
Chiun-Chien Huang; Poh-Hong Lim; Andrew C. Hall; Chien-Ning Huang
Source:
Life sciences 2011 v.88 no.23-24 pp. 1001-1008
ISSN:
0024-3205
Subject:
barium; epidermal growth factor receptors; genistein; humans; mitogen-activated protein kinase; phosphatidylinositol 3-kinase; potassium; potassium chloride; protein kinase C
Abstract:
... AIMS: KCl cotransport is believed to be involved in volume regulation in various erythroid cells of vertebrates, although the mechanism of activation and the role of the signaling elements involved remain uncertain. In this study, we characterized KCl cotransport activated by hypo-osmotic stress, and clarified several signaling elements involved in the regulation of this pathway within the human e ...
DOI:
10.1016/j.lfs.2011.03.004

12. A lysine-free mutant of epidermal growth factor as targeting moiety of a targeted toxin

Author:
Christopher Bachran; Stefanie Schneider; Sebastian B. Riese; Diana Bachran; Romy Urban; Nicole Schellmann; Claudia Zahn; Mark Sutherland; Hendrik Fuchs
Source:
Life sciences 2011 v.88 no.5-6 pp. 226-232
ISSN:
0024-3205
Subject:
antibodies; arginine; cytotoxicity; drugs; dyes; enzyme activity; epidermal growth factor; epidermal growth factor receptors; humans; mutants; mutation; therapeutics; uterine cervical neoplasms
Abstract:
... AIMS: Elevated levels of epidermal growth factor (EGF) receptor are observed on several human tumors, e.g. cervical carcinoma and mamma carcinomas. The natural ligand EGF is an alternative to established antibodies and tyrosine kinase inhibitors for targeting EGF receptor-overexpressing tumor cells for therapy. Conjugations of compounds to EGF lack the necessary homogeneity for an intended applica ...
DOI:
10.1016/j.lfs.2010.11.012

13. A mechanism by which the osmotic laxative magnesium sulphate increases the intestinal aquaporin 3 expression in HT-29 cells

Author:
Nobutomo Ikarashi; Toshihide Mochiduki; Ayaka Takasaki; Takashi Ushiki; Kohta Baba; Makoto Ishii; Toshiyuki Kudo; Kiyomi Ito; Takahiro Toda; Wataru Ochiai; Kiyoshi Sugiyama
Source:
Life sciences 2011 v.88 no.3-4 pp. 194-200
ISSN:
0024-3205
Subject:
Western blotting; adenylate cyclase; aquaporins; cAMP-dependent protein kinase; colon; colorectal neoplasms; gene expression; humans; magnesium; magnesium sulfate; messenger RNA; osmotic pressure; phosphorylation; protein synthesis; reverse transcriptase polymerase chain reaction; transcription (genetics)
Abstract:
... AIMS: We have suggested that an osmotic laxative, magnesium sulphate (MgSO₄), may act as a cathartic in a very rational manner by increasing the aquaporin 3 (AQP3) expression level and by changing osmotic pressure in the colon. In this study, we examined the mechanism by which MgSO₄ increases the intestinal AQP3 expression level by using the human colon cancer HT-29 cell line. MAIN METHODS: After ...
DOI:
10.1016/j.lfs.2010.11.013

14. A new approach regarding the treatment of preeclampsia and preterm labor

Author:
Shigehiko Mizutani; John Wright; Hiroshi Kobayashi
Source:
Life sciences 2011 v.88 no.1-2 pp. 17-23
ISSN:
0024-3205
Subject:
bioactive properties; fetus; leucyl aminopeptidase; magnesium sulfate; mice; molecular weight; morbidity; mortality; parturition; peptide hormones; pre-eclampsia; pregnancy; rats
Abstract:
... Both preeclampsia and preterm delivery are important complications in pregnancy and are leading causes for maternal and perinatal morbidity and mortality. The underlying molecular mechanisms of both diseases remain unknown, thus treatments (beta2-stimulants and magnesium sulfate) are essentially symptomatic. Both molecules have molecular weights less than 5–8kDa and cross the placental barrier thu ...
DOI:
10.1016/j.lfs.2010.10.013

15. A pilot study on the association between double positive Chlamydia pneumoniae serology and serum lipid indices alterations in obese adult females

Author:
Agnieszka Jaworowska; Grzegorz Bazylak
Source:
Life sciences 2011 v.89 no.23-24 pp. 854-861
ISSN:
0024-3205
Subject:
Chlamydophila pneumoniae; adults; antibodies; blood lipids; blood serum; body mass index; enzyme-linked immunosorbent assay; females; high density lipoprotein cholesterol; immunoglobulin A; immunoglobulin G; lifestyle; low density lipoprotein; overweight; questionnaires; regression analysis; serology; triacylglycerols; urban areas
Abstract:
... AIMS: To study the relationship between Chlamydia pneumoniae serologic status and serum lipid abnormalities in apparently healthy obese female subjects living in urban areas. MAIN METHODS: Serum samples from 117 apparently healthy females (mean age 50years), classified as overweight/obese (OW/OB,n=74) or normal weight (NW,n=43) according to their body mass index (BMI), were tested for specific IgG ...
DOI:
10.1016/j.lfs.2011.09.013

16. A prospective on drug abuse-associated epigenetics and HIV-1 replication

Author:
Jui Pandhare; Chandravanu Dash
Source:
Life sciences 2011 v.88 no.21-22 pp. 995-999
ISSN:
0024-3205
Subject:
HIV infections; Human immunodeficiency virus 1; cocaine; disease course; disease resistance; epigenetics; illicit drugs
Abstract:
... Drugs of abuse serve as cofactors to susceptibility to HIV infection and disease progression. Although clinical reports indicate association between HIV/AIDS and drug use, the molecular mechanism of infection susceptibility and disease progression remains unclear. Drugs such as cocaine exert their addictive effects in part by epigenetic mechanisms. Given that epigenetic modifications play an impor ...
DOI:
10.1016/j.lfs.2010.10.005
PubMed:
20951145
PubMed Central:
PMC3046297

17. AGE formation blockade with aminoguanidine does not ameliorate chronic allograft nephropathy

Author:
Christoph Schmaderer; Chang Jiang Xing; Gaby Anderson; Rob Hermans; Jens Lutz; Uwe Heemann; Marcus Baumann
Source:
Life sciences 2011 v.89 no.11-12 pp. 349-354
ISSN:
0024-3205
Subject:
blood serum; allografting; fibrosis; body weight; immunohistochemistry; lipid peroxidation; oxidative stress; inflammation; cardiomyocytes; kidney transplant; enzyme-linked immunosorbent assay; tandem mass spectrometry; hydroxyproline; advanced glycation end products; kidneys; kidney diseases; blood pressure; echocardiography; hypertrophy
Abstract:
... AIMS: Advanced glycation end products (AGEs) are produced by glycoxidation and lipid peroxidation. AGEs induce oxidative stress and inflammation, and accumulate in tubular cells after kidney transplantation. We hypothesize that the AGE formation blocker aminoguanidine (AG) reduces AGE formation and improves renal transplant function. MAIN METHODS: Fisher 344 kidneys were orthotopically transplante ...
DOI:
10.1016/j.lfs.2011.06.012

18. AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle

Author:
Ayako Furugen; Masaki Kobayashi; Katsuya Narumi; Meguho Watanabe; Sho Otake; Shirou Itagaki; Ken Iseki
Source:
Life sciences 2011 v.88 no.3-4 pp. 163-168
ISSN:
0024-3205
Subject:
AMP-activated protein kinase; Western blotting; glucose transporters; in vitro studies; in vivo studies; messenger RNA; quantitative polymerase chain reaction; rats; skeletal muscle
Abstract:
... AIMS: The aim of this study was to determine the effect of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, on monocarboxylate transporter 4 (MCT4) expression in rat skeletal muscle and a prototypic embryonal rhabdomyosarcoma cell line (RD cells). MAIN METHODS: We examined the alteration in Glucose transporter 4 (GLUT4) and MCT4 mRNA le ...
DOI:
10.1016/j.lfs.2010.11.003

19. Aberrant expression of imprinted genes in post-implantation rat embryos

Author:
Neelam A. Kedia-Mokashi; Raja Mugasimangalam; Mohammed Aiyaz; Srabani Mukherjee; N.H. Balasinor
Source:
Life sciences 2011 v.88 no.13-14 pp. 634-643
ISSN:
0024-3205
Subject:
adulthood; adults; cell cycle; embryonic mortality; flow cytometry; gene expression; gene expression regulation; genes; insulin; males; microarray technology; progeny; rats; reverse transcriptase polymerase chain reaction; sires; spermatogenesis; spermatozoa; tamoxifen
Abstract:
... AIM: Imprinted genes are known regulators of embryo growth. Studies from our laboratory have demonstrated that treatment of adult male rats with tamoxifen increased post-implantation loss at around midgestation. Expression of insulin like growth factor 2 (Igf2), a paternally expressed imprinted gene was down-regulated in the resorbing embryos obtained at embryonic day 13. Hypomethylation of Igf2-H ...
DOI:
10.1016/j.lfs.2011.02.005

20. Absence of equilibrative nucleoside transporter 1 in ENT1 knockout mice leads to altered nucleoside levels following hypoxic challenge

Author:
Jennifer B. Rose; Zlatina Naydenova; Andrew Bang; Azza Ramadan; Jost Klawitter; Kristin Schram; Gary Sweeney; Almut Grenz; Holger Eltzschig; James Hammond; Doo-Sup Choi; Imogen R. Coe
Source:
Life sciences 2011 v.89 no.17-18 pp. 621-630
ISSN:
0024-3205
Subject:
adenosine; phenotype; transporters; adenosine diphosphate; polymerase chain reaction; inosine monophosphate; cardiomyocytes; adenosine monophosphate; gene expression; high performance liquid chromatography; dipyridamole; genes; mice; receptors; oxygen; adults
Abstract:
... AIMS: Equilibrative nucleoside transporters (ENT) modulate the flux of adenosine. The ENT1-null (KO) mouse heart is endogenously cardioprotected but the cellular basis of this phenotype is unknown. Therefore, we investigated the cellular mechanisms underlying ENT1-mediated cardioprotection. MAIN METHODS: Circulating adenosine levels were measured in WT and KO mice. Cellular levels of nucleosides a ...
DOI:
10.1016/j.lfs.2011.08.007

21. Activation of Notch1 signaling by marrow-derived mesenchymal stem cells through cell–cell contact inhibits proliferation of hepatic stellate cells

Author:
Si Chen; linan Xu; Nan Lin; Weidong Pan; Kunpeng Hu; Ruiyun Xu
Source:
Life sciences 2011 v.89 no.25-26 pp. 975-981
ISSN:
0024-3205
Subject:
actin; cell growth; coculture; fibrosis; growth retardation; liver cirrhosis; messenger RNA; models; muscles; phosphatidylinositol 3-kinase; signal transduction; small interfering RNA; stem cells
Abstract:
... AIMS: Bone marrow-derived mesenchymal stem cells (BMSCs) have been reported in many studies to reduce liver fibrosis. Apart from the paracrine mechanism by which the antifibrotic effects of BMSCs inhibit activated hepatic stellate cells (HSCs), the effects of direct interplay and juxtacrine signaling between the two cell types are poorly understood. The purpose of this study was to explore the und ...
DOI:
10.1016/j.lfs.2011.10.012

22. Adenosine A₂AR modulates cardiovascular function by activating ERK1/2 signal in the rostral ventrolateral medulla of acute myocardial ischemic rats

Author:
Meiyan Jiang; Chengrong Zhang; Jin Wang; Jun Chen; Chunmei Xia; Dongshu Du; Na Zhao; Yinxiang Cao; Linlin Shen; Danian Zhu
Source:
Life sciences 2011 v.89 no.5-6 pp. 182-187
ISSN:
0024-3205
Subject:
Western blotting; adenosine; agonists; animal models; antagonists; coronary vessels; heart rate; immunohistochemistry; messenger RNA; mitogen-activated protein kinase; neurons; phosphorylation; protein synthesis; quantitative polymerase chain reaction; rats; receptors
Abstract:
... AIMS: To investigate the cardiovascular regulatory mechanism of adenosine A₂A receptor (A₂AR) in the rostral ventrolateral medulla (RVLM) in acute myocardial ischemic (AMI) rats. MAIN METHODS: The animal model of AMI was established by ligating the left anterior descending coronary artery (LAD). The A₂AR expression was examined by immunohistochemistry, western blot and real-time PCR. CGS21680 and ...
DOI:
10.1016/j.lfs.2011.06.003

23. Age-dependent effect of ouabain on renal Na⁺,K⁺-ATPase

Author:
E. Silva; M.P. Serrão; P. Soares-da-Silva
Source:
Life sciences 2011 v.88 no.15-16 pp. 719-724
ISSN:
0024-3205
Subject:
electrolytes; enzyme activity; excretion; ouabain; proximal tubules; rats; renal function; sodium; sodium-potassium-exchanging ATPase; systolic blood pressure
Abstract:
... AIMS: This study examines the effect of chronic ouabain-treatment on renal Na⁺ handling in 12-week and 52-week old rats. MAIN METHODS: Wistar Kyoto rats aged 5weeks or 45weeks were treated with ouabain or vehicle during 7weeks. Blood pressure was measured in conscious animals throughout the study. After 7weeks of treatment urinary electrolyte concentration, Na⁺,K⁺-ATPase activity and α₁-subunit ex ...
DOI:
10.1016/j.lfs.2011.02.012

24. Agmatine enhances neurogenesis by increasing ERK1/2 expression, and suppresses astrogenesis by decreasing BMP 2,4 and SMAD 1,5,8 expression in subventricular zone neural stem cells

Author:
Ho Won Song; Bokara Kiran Kumar; Sa Hyun Kim; Yong Heui Jeon; Yoon Azalea Lee; Won Teak Lee; Kyung Ah Park; Jong Eun Lee
Source:
Life sciences 2011 v.89 no.13-14 pp. 439-449
ISSN:
0024-3205
Subject:
Western blotting; agmatine; bone morphogenetic proteins; caspase-3; cell death; cell proliferation; central nervous system; culture media; epidermal growth factor; fibroblast growth factor 2; models; neurogenesis; phosphotransferases (kinases); protein synthesis; silver; stem cells
Abstract:
... AIM: Our study aimed to demonstrate whether agmatine (Ag) could regulate proliferation and cell fate determination of subventricular zone neural stem cells (SVZ NSCs). MAIN METHODS: SVZ NSCs were grown in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) (20ng/ml) until 4days in vitro (DIV) and later the culture medium was replaced without EGF and bFGF until 1 ...
DOI:
10.1016/j.lfs.2011.07.003

25. Aloe-emodin as antiproliferative and differentiating agent on human U937 monoblastic leukemia cells

Author:
Claudio Tabolacci; Serafina Oliverio; Alessandro Lentini; Stefania Rossi; Alice Galbiati; Carla Montesano; Palma Mattioli; Bruno Provenzano; Francesco Facchiano; Simone Beninati
Source:
Life sciences 2011 v.89 no.21-22 pp. 812-820
ISSN:
0024-3205
Subject:
protoporphyrin; polyamines; surface antigens; metabolism; humans; leukemia; melanoma; monocytes; protein-glutamine gamma-glutamyltransferase; macrophages; cytokines; nitroblue tetrazolium; enzyme activity; anthraquinones; apoptosis; anticarcinogenic activity
Abstract:
... AIMS: Aloe-emodin (AE), a plant derived anthraquinone, has been shown to have anticancer activity in various human cancer cell lines. We have recently reported that AE possesses a differentiative potential on melanoma cells. The purpose of this study was to investigate the possible modulation of defined markers of monocytic differentiation of AE on human U937 cell line. MAIN METHODS: U937 cells di ...
DOI:
10.1016/j.lfs.2011.09.008

26. Alterations in anxiety-like behavior following knockout of the uncoupling protein 2 (ucp2) gene in mice

Author:
Ulrike Gimsa; Ellen Kanitz; Winfried Otten; Caroline Aheng; Margret Tuchscherer; Daniel Ricquier; Bruno Miroux; Saleh M. Ibrahim
Source:
Life sciences 2011 v.89 no.19-20 pp. 677-684
ISSN:
0024-3205
Subject:
CD4-positive T-lymphocytes; Western blotting; anxiety; blood serum; brain; corticosterone; enzyme-linked immunosorbent assay; flow cytometry; genes; high performance liquid chromatography; immune response; lupus erythematosus; mice; oxidative stress; phenotype; psychological stress; radioimmunoassays; rheumatoid arthritis; sclerosis; splenocytes; stress response; tumor necrosis factor-alpha
Abstract:
... AIMS: Uncoupling protein 2 (UCP2) is a mitochondrial protein that reduces oxidative stress and has a protective function in chronic inflammatory diseases such as multiple sclerosis, rheumatoid arthritis and systemic lupus erythematosus. UCP2 is strongly expressed in areas implicated in the central regulation of stress and anxiety. Therefore, we compared the neuroendocrine regulation of stress resp ...
DOI:
10.1016/j.lfs.2011.08.009

27. Altered intestinal P-glycoprotein expression levels in a monosodium glutamate-induced obese mouse model

Author:
Ayaka Nawa; Wakako Fujita-Hamabe; Shogo Tokuyama
Source:
Life sciences 2011 v.89 no.23-24 pp. 834-838
ISSN:
0024-3205
Subject:
Western blotting; animal disease models; blood glucose; blood serum; blood-brain barrier; body mass index; drugs; duodenum; hyperglycemia; hyperinsulinemia; ileum; insulin; jejunum; kidneys; liver; mice; monosodium glutamate; nitric oxide synthase; noninsulin-dependent diabetes mellitus; obesity; streptozotocin
Abstract:
... AIMS: P-glycoprotein (P-gp) is an important drug efflux transporter located in many tissues such as the blood–brain barrier, intestines, liver and kidneys. We have previously reported that ileal P-gp expression levels decrease via a nitric oxide synthase (NOS)-mediated pathway in a streptozotocin (STZ)-induced type 1 diabetic mouse model. Herein, our objective was to assess whether there are diffe ...
DOI:
10.1016/j.lfs.2011.08.019

28. Andrographolide and 14-Deoxy-11, 12-Didehydroandrographolide inhibit cytochrome P450s in HepG2 hepatoma cells

Author:
Jer Ping Ooi; Masanori Kuroyanagi; Shaida Fariza Sulaiman; Tengku Sifzizul Tengku Muhammad; Mei Lan Tan
Source:
Life sciences 2011 v.88 no.9-10 pp. 447-454
ISSN:
0024-3205
Subject:
Andrographis paniculata; Western blotting; cytochrome P-450; dexamethasone; diterpenoids; enzyme activity; enzymes; hepatoma; human cell lines; messenger RNA; protein synthesis; reverse transcriptase polymerase chain reaction
Abstract:
... AIMS: Cytochrome P450 (CYP) enzymes have been implicated in a large number of preventable drug–herb interactions. Andrographis paniculata Nees, a tropical herb widely used for various health conditions contains two major diterpenoids, andrographolide and 14-Deoxy-11, 12-Didehydroandrographolide. These compounds were evaluated systematically for their effects on CYP1A2, CYP2D6 and CYP3A4 expression ...
DOI:
10.1016/j.lfs.2010.12.019

29. Angiotensin receptor blockade improves the net balance of cardiac Ca²⁺ handling-related proteins in sympathetic hyperactivity-induced heart failure

Author:
Julio C.B. Ferreira; Jose B.N. Moreira; Juliane C. Campos; Marcelo G. Pereira; Katt C. Mattos; Marcele A. Coelho; Patricia C. Brum
Source:
Life sciences 2011 v.88 no.13-14 pp. 578-585
ISSN:
0024-3205
Subject:
Ca2-transporting ATPase; Western blotting; adenosinetriphosphatase; adrenergic receptors; angiotensin II; calcium; cardiomyocytes; echocardiography; fibrosis; genetic background; heart failure; hypertrophy; mice; morphometry; protein kinases; sarcoplasmic reticulum; therapeutics
Abstract:
... AIMS: The clinical benefits of angiotensin II type 1 (AT1) receptor blockers (ARB) in heart failure (HF) include cardiac anti-remodeling and improved ventricular function. However, the cellular mechanisms underlying the benefits of ARB on ventricular function need to be better clarified. In the present manuscript, we evaluated the effects of AT1 receptor blockade on the net balance of Ca²⁺ handlin ...
DOI:
10.1016/j.lfs.2011.01.009

30. Anti-inflammatory effect of transduced PEP-1-Cyclophilin A in Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate-induced mice

Author:
Min Jung Lee; Dae Won Kim; Eun Jeong Sohn; Hoon Jae Jeong; Min Jea Shin; Hye Won Kang; Eun Hee Ahn; Soon Won Kwon; Young Nam Kim; Moo Ho Won; Joon Kim; Sung-Woo Cho; Tae-Cheon Kang; Kyu Hyung Han; Jinseu Park; Won Sik Eum; Soo Young Choi
Source:
Life sciences 2011 v.89 no.23-24 pp. 896-904
ISSN:
0024-3205
Subject:
Western blotting; animal models; anti-inflammatory activity; cyclophilins; cyclosporine; cytokines; enzyme-linked immunosorbent assay; gel electrophoresis; gene expression; genes; humans; immunohistochemistry; inflammation; messenger RNA; mice; mitogen-activated protein kinase; prostaglandin synthase; protective effect; protein synthesis; reverse transcriptase polymerase chain reaction; transcription factor NF-kappa B
Abstract:
... AIMS: Cyclophilin A (CypA) is an immunophilin that acts as a receptor for the immunosuppressant drug cyclosporine A (CsA). CypA has emerged as a potential drug target for several inflammatory diseases, although the details of its mechanism are unclear. We examined the protective effects of CypA on inflammation in Raw 264.7 cells and animal models. MAIN METHODS: A human CypA gene was fused with a p ...
DOI:
10.1016/j.lfs.2011.09.021

31. Anti-tussive and bronchodilator mechanisms of action for the enaminone E121

Author:
Ahmed Z. El-Hashim; Ivan O. Edafiogho; Sahar M. Jaffal; Mariam H. Yousif; Charles I. Ezeamuzie; Samuel B. Kombian
Source:
Life sciences 2011 v.89 no.11-12 pp. 378-387
ISSN:
0024-3205
Subject:
antagonists; bronchodilators; cough; eosinophils; glibenclamide; guinea pigs; humans; mechanism of action; models; muscle contraction; pretreatment; propranolol; receptors; smooth muscle; therapeutics
Abstract:
... AIMS: In this study, we investigated whether the enaminone, E121, has anti-tussive effects in a guinea pig model of cough, and if so, whether this effect is mediated via a central or peripheral site of action. We also assessed whether E121 has bronchodilator effects and the molecular mechanisms underlying any anti-tussive and/or bronchodilator effects. MAIN METHODS: Whole body plethysmography was ...
DOI:
10.1016/j.lfs.2011.07.007

32. Associations between use of crack cocaine and HIV-1 disease progression: Research findings and implications for mother-to-infant transmission

Author:
Judith A. Cook
Source:
Life sciences 2011 v.88 no.21-22 pp. 931-939
ISSN:
0024-3205
Subject:
Human immunodeficiency virus 1; antiretroviral agents; cocaine; disease course; epidemiological studies; immune response; immune system; neonates; pathogenesis; therapeutics; virus replication; women
Abstract:
... Recent in vitro and in vivo research has suggested that cocaine has a direct effect on the pathogenesis of AIDS. These findings are confirmed by epidemiological studies linking the use of injected, inhaled, and smoked (crack) cocaine and indicators of HIV disease progression, even among adherent users of highly active antiretroviral therapy. Recent studies of vertical HIV transmission suggest that ...
DOI:
10.1016/j.lfs.2011.01.003
PubMed:
21219914
PubMed Central:
PMC3100443

33. Astrocyte activation but not neuronal impairment occurs in the hippocampus of mice after 2weeks of d-galactose exposure

Author:
Wei Wu; Min Li; Lei Liu; Junying Gao; Hui Kong; Jiong Ding; Gang Hu; Ming Xiao
Source:
Life sciences 2011 v.89 no.11-12 pp. 355-363
ISSN:
0024-3205
Subject:
adults; astrocytes; cell death; dendrites; electron microscopy; glutamic acid; hippocampus; immunohistochemistry; intraperitoneal injection; mice; neurodegenerative diseases
Abstract:
... AIMS: The objective of this study is to prove that activation of astrocytes precedes neuron cell death in the neurodegenerative process induced by d-galactose (d-gal) exposure. MAIN METHODS: Male adult mice were given intraperitoneal injection of d-gal (200mg/kg per day) for 2weeks. The whole brain homogenate and hippocampal sections were then prepared for biochemical analyses, immunohistochemistr ...
DOI:
10.1016/j.lfs.2011.06.028

34. Atorvastatin ameliorates tissue damage of obstructed ureter in rats

Author:
Yen-Hwang Chuang; Wan-Long Chuang; Shu-Pin Huang; Ching-Kuan Liu; Chun-Hsiung Huang
Source:
Life sciences 2011 v.89 no.21-22 pp. 795-805
ISSN:
0024-3205
Subject:
apoptosis; fibrosis; interleukin-1beta; interleukin-6; muscles; proliferating cell nuclear antigen; rats; smooth muscle; tumor necrosis factor-alpha; ureter
Abstract:
... AIMS: To investigate the effects of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor on the tissue damage and fibrosis of obstructed ureters, 80 rats were studied. MAIN METHODS: Atorvastatin, a HMG-CoA reductase inhibitor, was administered to 40 rats at the dose of 20mg/kg per day 1day before unilateral ligation of ureters and every day thereafter. The other rats served as contr ...
DOI:
10.1016/j.lfs.2011.09.010

35. Automated experimental system capturing three behavioral components during murine forced swim test

Author:
Etsuko Hayashi; Midori Shimamura; Kazuyoshi Kuratani; Mine Kinoshita; Hideaki Hara
Source:
Life sciences 2011 v.88 no.9-10 pp. 411-417
ISSN:
0024-3205
Subject:
acute effects; antidepressants; correlation; mice; swimming
Abstract:
... AIMS: An automated experimental system applying a commercially available video image analyzer was developed for the simultaneous detection and measurement of three behavioral components; immobility, swimming (horizontal movements) and climbing (vertical movements) that occur in the murine forced swim test (FST). The system was validated using four typical antidepressants. MAIN METHODS: System vali ...
DOI:
10.1016/j.lfs.2010.12.016

36. BB rat Gimap gene expression in sorted lymphoid T and B cells

Author:
Daniel H. Moralejo; Jessica M. Fuller; Elizabeth A. Rutledge; Brian Van Yserloo; Ruth A. Ettinger; Richard Jensen; William Osborne; Anne Kwitek; Åke Lernmark
Source:
Life sciences 2011 v.89 no.19-20 pp. 748-754
ISSN:
0024-3205
Subject:
B-lymphocytes; T-lymphocytes; cell viability; frameshift mutation; gene expression; genes; homeostasis; insulin-dependent diabetes mellitus; lymph nodes; macrophages; natural killer cells; polymerase chain reaction; quantitative analysis; quantitative trait loci; rats; spleen; thymocytes
Abstract:
... AIMS: The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quant ...
DOI:
10.1016/j.lfs.2011.08.016
PubMed:
21925515
PubMed Central:
PMC3264544

37. Baicalin upregulates the genetic expression of antioxidant enzymes in Type-2 diabetic Goto-Kakizaki rats

Author:
Viduranga Y. Waisundara; Sing Yung Siu; Annie Hsu; Dejian Huang; Benny K.H. Tan
Source:
Life sciences 2011 v.88 no.23-24 pp. 1016-1025
ISSN:
0024-3205
Subject:
Western blotting; antioxidants; baicalin; blood; blood glucose; blood lipids; catalase; cholesterol; enzyme activity; gene expression regulation; glutathione peroxidase; heart; hyperglycemia; liver; metformin; microarray technology; noninsulin-dependent diabetes mellitus; oxidative stress; pancreas; rats; reverse transcriptase polymerase chain reaction; superoxide dismutase; triacylglycerols
Abstract:
... AIM: The primary purpose of this study was to characterize and investigate the antioxidant and anti-diabetic activities of the flavonoid baicalin in type 2 diabetic Goto-Kakizaki rats. MAIN METHODS: Four groups of Goto-Kakizaki rats (n=6) were subjected to the following oral treatments for 30days: (1) metformin — 500mg/kg (2) baicalin — 120mg/kg (3) metformin 500mg/kg and baicalin — 120mg/kg (4) v ...
DOI:
10.1016/j.lfs.2011.03.009

38. Beneficial health effects of lupeol triterpene: A review of preclinical studies

Author:
Hifzur Rahman Siddique; Mohammad Saleem
Source:
Life sciences 2011 v.88 no.7-8 pp. 285-293
ISSN:
0024-3205
Subject:
alpha-glucosidase; animal models; anticholesteremic agents; arthritis; cardiovascular diseases; diabetes; fruits; human diseases; humans; interleukin-2; interleukin-4; interleukin-5; intravenous injection; kidney diseases; lupeol; mechanism of action; proteinases; tissue repair; vegetables
Abstract:
... Since ancient times, natural products have been used as remedies to treat human diseases. Lupeol, a phytosterol and triterpene, is widely found in edible fruits, and vegetables. Extensive research over the last three decades has revealed several important pharmacological activities of lupeol. Various in vitro and preclinical animal studies suggest that lupeol has a potential to act as an anti-infl ...
DOI:
10.1016/j.lfs.2010.11.020

39. Beta blocker metoprolol protects against contractile dysfunction in rats after coronary microembolization by regulating expression of myocardial inflammatory cytokines

Author:
Yongguang Lu; Lang Li; Xianming Zhao; Weiqiang Huang; Weiming Wen
Source:
Life sciences 2011 v.88 no.23-24 pp. 1009-1015
ISSN:
0024-3205
Subject:
anti-inflammatory activity; aorta; echocardiography; interleukin-10; interleukin-1beta; intravenous injection; messenger RNA; necrosis; rats; tumor necrosis factor-alpha
Abstract:
... AIMS: To examine the effects of metoprolol on expression of myocardial inflammatory cytokines and myocardial function in rats following coronary microembolization (CME). MAIN METHODS: Male rats were randomly assigned to receive either sham-operation (control group), CME plus saline (CME group), or CME plus metoprolol (metoprolol group). CME was induced by injecting 3000 polyethylene microspheres ( ...
DOI:
10.1016/j.lfs.2011.03.012

40. Biological and chemical studies on aryl hydrocarbon receptor induction by the p53 inhibitor pifithrin-α and its condensation product pifithrin-β

Author:
María Luisa Fernández-Cruz; Ana Valdehita; Mercedes Alonso; Enrique Mann; Bernardo Herradón; José María Navas
Source:
Life sciences 2011 v.88 no.17-18 pp. 774-783
ISSN:
0024-3205
Subject:
Oncorhynchus mykiss; acetylation; chemical analysis; drugs; hepatoma; neurodegenerative diseases; nitrogen; rats; therapeutics
Abstract:
... AIMS: Pifithrin α (PFTα), an inhibitor of the p53 protein, is regarded as a lead compound for cancer and neurodegenerative disease therapy. There is some evidence that this compound activates the aryl hydrocarbon receptor (AhR) in a complete independent way of the p53 inhibition and that it is easily converted to its condensation product pifithrin β (PFTβ). The aim of this study was to explore the ...
DOI:
10.1016/j.lfs.2011.02.019

41. Biphasic effect of EGb761 on simulated ischemia-induced rat BMSC survival in vitro and in vivo

Author:
Shibao Li; Derong Tang; Zhiwei Xue; Zhifeng Zhang; Xiaojing Sun; Yuepeng Liu; Hongyan Dong; Xiaoxing Yin; Zhongming Zhang
Source:
Life sciences 2011 v.88 no.19-20 pp. 853-863
ISSN:
0024-3205
Subject:
Ginkgo biloba; apoptosis; blood serum; bone marrow; caspase-3; caspase-9; hypoxia; leaves; models; myocardial infarction; myocardium; rats; signal transduction; stem cells
Abstract:
... AIMS: The standardized extract from the leaves of Ginkgo biloba (EGb761) is applied as a phyto-pharmacon in therapy of diverse cardiovascular disorders. However, the effects of EGb761 on bone-marrow mesenchymal stem cells (BMSCs) transplanted into the ischemic myocardium currently remain uncertain. In this study, the dosage-effects of EGb761 on BMSC survival in vitro and in vivo were investigated. ...
DOI:
10.1016/j.lfs.2011.03.002

42. Bone marrow mononuclear cells reduce seizure frequency and improve cognitive outcome in chronic epileptic rats

Author:
Gianina Teribele Venturin; Samuel Greggio; Daniel Rodrigo Marinowic; Gabriele Zanirati; Martín Cammarota; Denise Cantarelli Machado; Jaderson Costa DaCosta
Source:
Life sciences 2011 v.89 no.7-8 pp. 229-234
ISSN:
0024-3205
Subject:
memory; genetically modified organisms; green fluorescent protein; neurons; intravenous injection; fibroblasts; polymerase chain reaction; mice; stem cells; bone marrow; seizures; epilepsy; patients; cognition; diazepam; disease course; rats
Abstract:
... AIMS: Epilepsy affects 0.5–1% of the world's population, and approximately a third of these patients are refractory to current medication. Given their ability to proliferate, differentiate and regenerate tissues, stem cells could restore neural circuits lost during the course of the disease and reestablish the physiological excitability of neurons. This study verified the therapeutic potential of ...
DOI:
10.1016/j.lfs.2011.06.006

43. C-type natriuretic peptide does not attenuate the development of pulmonary hypertension caused by hypoxia and VEGF receptor blockade

Author:
Brian Casserly; Jeffrey M. Mazer; Alexander Vang; Elizabeth O. Harrington; James R. Klinger; Sharon Rounds; Gaurav Choudhary
Source:
Life sciences 2011 v.89 no.13-14 pp. 460-466
ISSN:
0024-3205
Subject:
animal models; blood serum; hemodynamics; humans; hypertension; hypertrophy; hypoxia; intravenous injection; lungs; natriuretic peptides; rats; vascular endothelial growth factor receptors; vascular endothelial growth factors
Abstract:
... AIMS: C-type natriuretic peptide (CNP) is a local regulator of vascular tone and remodeling in many vascular beds. However, the role of CNP in modulating pulmonary arterial hypertensive and vascular remodeling responses is unclear. The purpose of this study was to determine if CNP is capable of preventing the development of pulmonary hypertension (PH). MAIN METHODS: We used animal models of PH cau ...
DOI:
10.1016/j.lfs.2011.07.009
PubMed:
21820448
PubMed Central:
PMC3533228

44. CCK-2/gastrin receptor signaling pathway is significant for gemcitabine-induced gene expression of VEGF in pancreatic carcinoma cells

Author:
Hiroki Kato; Koichi Seto; Nobuyoshi Kobayashi; Koji Yoshinaga; Tim Meyer; Mineo Takei
Source:
Life sciences 2011 v.89 no.17-18 pp. 603-608
ISSN:
0024-3205
Subject:
antagonists; carcinogenesis; carcinoma; enzyme-linked immunosorbent assay; gene expression; humans; immunoglobulin G; mice; pancreatic neoplasms; patients; phosphatidylinositol 3-kinase; phosphorylation; quantitative analysis; reverse transcriptase polymerase chain reaction; signal transduction; therapeutics; vascular endothelial growth factors
Abstract:
... AIMS: As activation and overexpression of the cholecystokinin-2 (CCK-2)/gastrin receptor can lead to carcinogenesis, it has been explored as a therapeutic target in pancreatic cancer. We demonstrated that Z-360, a CCK-2/gastrin receptor antagonist, combined with gemcitabine prolonged survival and reduced gemcitabine-induced vascular endothelial growth factor (VEGF) expression in a pancreatic carci ...
DOI:
10.1016/j.lfs.2011.07.019

45. CDP-choline-induced contractions in the mouse gastric fundus through purinoceptors and Rho/Rho-kinase signalling

Author:
Ozge Guldali; Vahide Savci; Kansu Buyukafsar
Source:
Life sciences 2011 v.88 no.11-12 pp. 473-479
ISSN:
0024-3205
Subject:
acetylcholinesterase; antagonists; atropine; calcium; choline; citrates; cytidine; cytidine monophosphate; gastric fundus; metabolites; mice; nitric oxide synthase; pertussis toxin; phosphorylation; physostigmine; sodium; suramin; tetrodotoxin
Abstract:
... AIMS: This study aimed to investigate the effects of cytidine-5′-diphosphocholine (CDP-choline), an endogenous lipid precursor, on the reactivity of the mouse gastric fundus and to determine the mechanism(s) mediating its effects. MAIN METHODS: Possible contractile effect of CDP-choline (10⁻⁵–10⁻²M) was investigated in the absence and presence of a muscarinic receptor antagonist, atropine (3×10⁻⁶M ...
DOI:
10.1016/j.lfs.2011.01.002

46. Calcineurin inhibition by polaprezinc in rats with experimentally-induced colitis

Author:
Yajing Zhang; Shinichi Okamura; Tomohiro Kudo; Takashige Masuo; Masatomo Mori
Source:
Life sciences 2011 v.88 no.9-10 pp. 432-439
ISSN:
0024-3205
Subject:
T-lymphocytes; cell viability; colitis; colon; cyclosporine; gene expression; in vitro studies; inflammation; interleukin-2; intestinal mucosa; protein synthesis; rats; reverse transcriptase polymerase chain reaction; therapeutics; transcription factors; zinc
Abstract:
... AIMS: We investigated the therapeutic effect of polaprezinc (PZ), N-(3-aminopropionyl)-l-histidinato zinc, in rats with experimentally-induced colitis by focusing on calcineurin (CN) inhibition. CN plays a crucial role in T-cell activation and cytokine gene expression and is targeted by immunosuppressants such as cyclosporine and FK506. MAIN METHODS: Colitis was induced into male Wistar rats by tr ...
DOI:
10.1016/j.lfs.2010.12.018

47. Cardamonin suppresses nitric oxide production via blocking the IFN-γ/STAT pathway in endotoxin-challenged peritoneal macrophages of ICR mice

Author:
Amane Takahashi; Norio Yamamoto; Akira Murakami
Source:
Life sciences 2011 v.89 no.9-10 pp. 337-342
ISSN:
0024-3205
Subject:
Western blotting; anti-inflammatory activity; anti-inflammatory agents; body weight; chalcone; curcumin; cytokines; enzyme-linked immunosorbent assay; gene expression; gene expression regulation; genes; inducible nitric oxide synthase; inflammation; interferon-gamma; lipopolysaccharides; macrophages; messenger RNA; mice; nitric oxide; reverse transcriptase polymerase chain reaction; transcription factor NF-kappa B; turmeric
Abstract:
... AIMS: Overproduction of nitric oxide (NO) from inducible NO synthase (iNOS) plays many pivotal roles in various inflammatory diseases. In this study, we examined the effects of 6 flavonoids on lipopolysaccharide (LPS)-induced NO generation in macrophage (Mφ), and addressed molecular mechanisms of cardamonin. MAIN METHODS: Suppressive effects on NO generation in vitro were assayed in LPS-stimulated ...
DOI:
10.1016/j.lfs.2011.06.027

48. Casein-derived tripeptide Ile–Pro–Pro improves angiotensin-(1–7)- and bradykinin-induced rat mesenteric artery relaxation

Author:
Pauliina I. Ehlers; Laura Nurmi; Anu M. Turpeinen; Riitta Korpela; Heikki Vapaatalo
Source:
Life sciences 2011 v.88 no.5-6 pp. 206-211
ISSN:
0024-3205
Subject:
angiotensins; animal models; antagonists; bioactive properties; blood pressure; bradykinin; hypertension; isoleucine; mesenteric arteries; milk; peptidyl-dipeptidase A; proline; rats; renin-angiotensin system; tripeptides; vasodilation
Abstract:
... AIMS: Milk casein-derived bioactive tripeptides isoleucine–proline–proline (Ile–Pro–Pro) and valine–proline–proline (Val–Pro–Pro) lower blood pressure in animal models of hypertension and humans. In some studies, their angiotensin-converting enzyme (ACE)-inhibitory effect has been demonstrated. Besides classical ACE-angiotensin II-AT₁-receptor pathway (ACE-Ang II- AT₁), the significance of ACE2-an ...
DOI:
10.1016/j.lfs.2010.11.001

49. Casted-immobilization downregulates glucocorticoid receptor expression in rat slow-twitch soleus muscle

Author:
Shogo Sato; Hideki Suzuki; Hisaya Tsujimoto; Ken Shirato; Kaoru Tachiyashiki; Kazuhiko Imaizumi
Source:
Life sciences 2011 v.89 no.25-26 pp. 962-967
ISSN:
0024-3205
Subject:
Western blotting; corticosterone; cytosol; enzyme-linked immunosorbent assay; gene expression regulation; genes; glucocorticoid receptors; hypertrophy; messenger RNA; models; muscle protein; muscles; muscular atrophy; protein degradation; protein synthesis; rats; reverse transcriptase polymerase chain reaction; skeletal muscle
Abstract:
... AIMS: Glucocorticoids bind to the glucocorticoid receptor (GR) and increase catabolism of muscle proteins via the ubiquitin-proteasome pathway. Activation of β₂-adrenergic receptor (β₂-AR) in skeletal muscle has been shown to induce muscle hypertrophy by promoting muscle protein synthesis and/or attenuating protein degradation. The aim of this study was to investigate the correlation between disus ...
DOI:
10.1016/j.lfs.2011.10.008

50. Cell signaling in NMDA preconditioning and neuroprotection in convulsions induced by quinolinic acid

Author:
Patricia Cardoso Severino; Gabriele do Amaral Silva Muller; Samuel Vandresen-Filho; Carla Inês Tasca
Source:
Life sciences 2011 v.89 no.15-16 pp. 570-576
ISSN:
0024-3205
Subject:
brain; epilepsy; ischemia; models; neurodegenerative diseases; neuroprotective effect; pretreatment; seizures
Abstract:
... The search for novel, less invasive therapeutic strategies to treat neurodegenerative diseases has stimulated scientists to investigate the mechanisms involved in preconditioning. Preconditioning has been report to occur in many organs and tissues. In the brain, the modulation of glutamatergic transmission is an important and promising target to the use of effective neuroprotective agents. The glu ...
DOI:
10.1016/j.lfs.2011.05.014