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- Giuffrida, Francesca; Cruz-Hernandez, Cristina; Flück, Brigitte; Tavazzi, Isabelle; Thakkar, Sagar K.; Destaillats, Frédéric; Braun, Marcel
- Lipids 2013 v.48 no.10 pp. 1051-1058
- breast feeding; breast milk; chloroform; infants; inflammation; light scattering; liquid chromatography; methanol; milk fat; phosphatidylcholines; phosphatidylethanolamines; phosphatidylserines; sphingomyelins; volunteers
- ... Phospholipids are integral constituents of the milk fat globule membranes and they play a central role in infants’ immune and inflammatory responses. A methodology employing liquid chromatography coupled with evaporative light scattering detector has been optimized and validated to quantify the major phospholipids classes in human milk. Phospholipids were extracted using chloroform and methanol an ...
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- Kobayashi, Naoko; Noel, Eric A.; Barnes, Austin; Rosenberg, Julian; DiRusso, Concetta; Black, Paul; Oyler, George A.
- Lipids 2013 v.48 no.10 pp. 1035-1049
- Chlamydomonas reinhardtii; Chlorella; Parachlorella kessleri; carotenoids; fatty acid composition; free fatty acids; galactolipids; gas chromatography; high performance liquid chromatography; labor; light scattering; monitoring; nitrogen; oils; phospholipids; thin layer chromatography; triacylglycerols
- ... Triacylglycerol (TAG) analysis and quantification are commonly performed by first obtaining a purified TAG fraction from a total neutral lipid extract using thin-layer chromatography (TLC), and then analyzing the fatty acid composition of the purified TAG fraction by gas chromatography (GC). This process is time-consuming, labor intensive and is not suitable for analysis of small sample sizes or l ...
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