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1. Simultaneous identification and combined genotype effects analysis of pre-implantation protein 3 (prei3) and estrogen receptor (ESR) gene in pigs

Author:
Ye, L.Z.,   et al.   ; Niu, B.Y.; Luo, L.F.; Shao, G.C.; Mei, S.Q.; Deng, C.Y.; Jiang, S.W.; Xiong, Y.Z.; Li, F.E.;     Show all 9 Authors
Source:
Livestock science 2009 v.125 no.1 pp. 80-83
ISSN:
1871-1413
Subject:
gilts; loci; animal proteins; oviducts; hormone receptors; estrogens; genotype; genes; genetic techniques and protocols; reproductive traits; progeny testing; animal genetics
Abstract:
... A method allowing simultaneous genotyping of two loci: pre-implantation protein 3 (prei3) and estrogen receptor (ESR) was presented. In multiplex PCR amplification, two amplicons were simultaneously produced a 294bp fragment of prei3 gene and a 121bp fragment of ESR gene and were then subjected to ''one-tube'' restriction enzyme digestion with MspI and PvuII. A total of 92 sows from the 4th dam li ...
DOI:
10.1016/j.livsci.2009.02.017