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You searched for: Journal Marine biotechnology Remove constraint Journal: Marine biotechnology Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.15 no.5 Remove constraint Source: 2013 v.15 no.5
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1. Antifouling Activity of Secondary Metabolites Isolated from Chinese Marine Organisms

Author:
Li, Yong-Xin; Wu, Hui-Xian; Xu, Ying; Shao, Chang-Lun; Wang, Chang-Yun; Qian, Pei-Yuan
Source:
Marine biotechnology 2013 v.15 no.5 pp. 552-558
ISSN:
1436-2228
Subject:
Balanus; Phaeophycophyta; antifouling agents; biofouling; corals; diterpenoids; fungi; larvae; organotin compounds; polyketides; secondary metabolites; steroids; China
Abstract:
... Biofouling results in tremendous economic losses to maritime industries around the world. A recent global ban on the use of organotin compounds as antifouling agents has further raised demand for safe and effective antifouling compounds. In this study, 49 secondary metabolites, including diterpenoids, steroids, and polyketides, were isolated from soft corals, gorgonians, brown algae, and fungi col ...
DOI:
10.1007/s10126-013-9502-7
PubMed:
23613141

2. Comparative Genome Analysis of Fish and Human Isolates of Mycobacterium marinum

Author:
Kurokawa, Satoru; Kabayama, Jun; Hwang, Seong Don; Nho, Seong-Won; Hikima, Jun-ichi; Jung, Tae-Sung; Sakai, Masahiro; Kondo, Hidehiro; Hirono, Ikuo; Aoki, Takashi
Source:
Marine biotechnology 2013 v.15 no.5 pp. 596-605
ISSN:
1436-2228
Subject:
Mycobacterium marinum; chromosomes; fatty acids; fish; gene deletion; genes; hosts; humans; nucleotide sequences; pathogens; virulence
Abstract:
... Mycobacterium marinum is a major causative agent of mycobacteriosis in fish that has a broad range of hosts, including in human isolates. So far, genomic analyses have focused on the human isolate. Here, we compared the draft genome sequences of two strains of M. marinum isolated from fish (MB2 and Europe) with the M. marinum M isolated from humans. M. marinum MB2 and Europe have single, circular ...
DOI:
10.1007/s10126-013-9511-6
PubMed:
23728847

3. Delivery of Nucleic Acids, Proteins, and Nanoparticles by Arginine-Rich Cell-Penetrating Peptides in Rotifers

Author:
Liu, Betty Revon; Liou, Ji-Sing; Chen, Yung-Jen; Huang, Yue-Wern; Lee, Han-Jung
Source:
Marine biotechnology 2013 v.15 no.5 pp. 584-595
ISSN:
1436-2228
Subject:
Rotifera; green fluorescent protein; nanoparticles; nucleic acids; peptides; proteins; quantum dots; reporter genes
Abstract:
... Cell-penetrating peptides (CPPs) are a group of short, membrane-permeable cationic peptides that represent a nonviral technology for delivering nanomaterials and macromolecules into live cells. In this study, two arginine-rich CPPs, HR9 and IR9, were found to be capable of entering rotifers. CPPs were able to efficiently deliver noncovalently associated with cargoes, including plasmid DNAs, red fl ...
DOI:
10.1007/s10126-013-9509-0
PubMed:
23715807

4. Isolation of Fucoxanthin and Highly Unsaturated Monogalactosyldiacylglycerol from Brown Alga Fucus evanescens C Agardh and In Vitro Investigation of Their Antitumor Activity

Author:
Imbs, Tatiana I.; Ermakova, Svetlana P.; Fedoreyev, Sergey A.; Anastyuk, Stanislav D.; Zvyagintseva, Tatiana N.
Source:
Marine biotechnology 2013 v.15 no.5 pp. 606-612
ISSN:
1436-2228
Subject:
Fucus evanescens; anticarcinogenic activity; ethanol; growth retardation; human growth; inhibitory concentration 50; ionization; mass spectrometry; melanoma; nuclear magnetic resonance spectroscopy
Abstract:
... Fucoxanthin (FX) and highly unsaturated monogalactosyldiacylglycerol (MGDG) were isolated from the ethanol extract of brown alga Fucus evanescens. Their structures were identified by nuclear magnetic resonance, complemented by electrospray ionization mass spectrometry (ESIMS). MGDG was identified as 1-O-(5Z,8Z,11Z,14Z,17Z-eicosapentanoyl)-2-O-(6Z,9Z,12Z,15Z-octadecatetraenoyl)-3-O-β-D-galactopiran ...
DOI:
10.1007/s10126-013-9507-2
PubMed:
23748883

5. Mariculture and Natural Production of the Antitumoural (+)-Discodermolide by the Caribbean Marine Sponge Discodermia dissoluta

Author:
Ruiz, Cesar; Valderrama, Katherine; Zea, Sven; Castellanos, Leonardo
Source:
Marine biotechnology 2013 v.15 no.5 pp. 571-583
ISSN:
1436-2228
Subject:
bags; cell culture; clinical trials; mariculture; metabolites; survival rate; Caribbean; Colombia
Abstract:
... Biotechnological research on marine organisms, such as ex situ or in situ aquaculture and in vitro cell culture, is being conducted to produce bioactive metabolites for biomedical and industrial uses. The Caribbean marine sponge Discodermia dissoluta is the source of (+)-discodermolide, a potent antitumoural polyketide that has reached clinical trials. This sponge usually lives at depths greater t ...
DOI:
10.1007/s10126-013-9510-7
PubMed:
23728846

6. Marine-Derived Aspergillus Species as a Source of Bioactive Secondary Metabolites

Author:
Lee, Yoon Mi; Kim, Min Jeong; Li, Huayue; Zhang, Ping; Bao, Baoquan; Lee, Ka Jeong; Jung, Jee H.
Source:
Marine biotechnology 2013 v.15 no.5 pp. 499-519
ISSN:
1436-2228
Subject:
Aspergillus; bioactive properties; secondary metabolites
Abstract:
... This report reviews biologically active secondary metabolites from marine-derived members of the fungal genus Aspergillus. Pharmacological activities and biological roles of the secondary metabolites from marine-derived Aspergillus spp. were addressed in respect of pharmaceutical potential. ...
DOI:
10.1007/s10126-013-9506-3
PubMed:
23709045

7. Optimization of a Method for Chromatin Immunoprecipitation Assays in the Marine Invertebrate Chordate Ciona

Author:
Aihara, Hitoshi; Katikala, Lavanya; Zeller, Robert W.; Di Gregorio, Anna; Nibu, Yutaka
Source:
Marine biotechnology 2013 v.15 no.5 pp. 520-525
ISSN:
1436-2228
Subject:
Chordata; DNA-binding proteins; aquatic invertebrates; chromatin; system optimization
Abstract:
... Chromatin immunoprecipitation (ChIP) assays allow the efficient characterization of the in vivo occupancy of genomic regions by DNA-binding proteins and thus facilitate the prediction of cis-regulatory sequences in silico and guide their validation in vivo. For these reasons, these assays and their permutations (e.g., ChIP-on-chip and ChIP-sequencing) are currently being extended to several non-ma ...
DOI:
10.1007/s10126-013-9504-5
PubMed:
23592257
PubMed Central:
PMC3744622

8. Phylogenetically Diverse Denitrifying and Ammonia-Oxidizing Bacteria in Corals Alcyonium gracillimum and Tubastraea coccinea

Author:
Yang, Shan; Sun, Wei; Zhang, Fengli; Li, Zhiyong
Source:
Marine biotechnology 2013 v.15 no.5 pp. 540-551
ISSN:
1436-2228
Subject:
DNA libraries; RNA libraries; ammonia; ammonia monooxygenase; bacterial communities; community structure; corals; denitrification; denitrifying bacteria; genes; nitrite reductase; nitrogen; oxidation; ribosomal RNA; symbionts; East China Sea
Abstract:
... To date, the association of coral–bacteria and the ecological roles of bacterial symbionts in corals remain largely unknown. In particular, little is known about the community components of bacterial symbionts of corals involved in the process of denitrification and ammonia oxidation. In this study, the nitrite reductase (nirS and nirK) and ammonia monooxygenase subunit A (amoA) genes were used as ...
DOI:
10.1007/s10126-013-9503-6
PubMed:
23564007

9. Selenoneine, a Novel Selenium-Containing Compound, Mediates Detoxification Mechanisms against Methylmercury Accumulation and Toxicity in Zebrafish Embryo

Author:
Yamashita, Michiaki; Yamashita, Yumiko; Suzuki, Tamami; Kani, Yoko; Mizusawa, Nanami; Imamura, Shintaro; Takemoto, Kenji; Hara, Tatsuro; Hossain, Md. Anwar; Yabu, Takeshi; Touhata, Ken
Source:
Marine biotechnology 2013 v.15 no.5 pp. 559-570
ISSN:
1436-2228
Subject:
Danio rerio; blood cells; cations; excretion; fish consumption; humans; kidneys; mercury; methylmercury compounds; secretion; selenium; toxicity; tuna
Abstract:
... The selenium (Se)-containing antioxidant selenoneine (2-selenyl-Nα,Nα,Nα-trimethyl-L-histidine) has recently been discovered to be the predominant form of organic Se in tuna blood. Although dietary intake of fish Se has been suggested to reduce methylmercury (MeHg) toxicity, the molecular mechanism of MeHg detoxification by Se has not yet been determined. Here, we report evidence that selenoneine ...
DOI:
10.1007/s10126-013-9508-1
PubMed:
23709046
PubMed Central:
PMC3742965

10. Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems

Author:
Xiong, Feng; Wei, Zhi-Qiang; Zhu, Zuo-Yan; Sun, Yong-Hua
Source:
Marine biotechnology 2013 v.15 no.5 pp. 526-539
ISSN:
1436-2228
Subject:
Danio rerio; adults; eggs; females; gene expression; genes; hybrids; males; proteins; quantitative polymerase chain reaction; spermatozoa; transcription (genetics); vertebrates
Abstract:
... In zebrafish and other vertebrates, primordial germ cells (PGCs) are a population of embryonic cells that give rise to sperm and eggs in adults. Any type of genetically manipulated lines have to be originated from the germ cells of the manipulated founders, thus it is of great importance to establish an effective technology for highly specific PGC-targeted gene manipulation in vertebrates. In the ...
DOI:
10.1007/s10126-013-9505-4
PubMed:
23535913