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You searched for: Journal name Marine biotechnology Remove constraint Journal name: Marine biotechnology Publication year rev 7991-2009 Remove constraint Publication year rev: 7991-2009 Source 2009 v.11 no.1 Remove constraint Source: 2009 v.11 no.1
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1. A Multiplex PCR Method for Rapid Identification of Brachionus Rotifers

Author:
Vasileiadou, Kalliopi; Papakostas, Spiros; Triantafyllidis, Alexander; Kappas, Ilias; Abatzopoulos, Theodore J.
Source:
Marine biotechnology 2009 v.11 no.1 pp. 53-61
ISSN:
1436-2228
Subject:
Brachionus; DNA; biotypes; cytochrome-c oxidase; genetic polymorphism; hatcheries; polymerase chain reaction; rapid methods; Nevada
Abstract:
... Cryptic species are increasingly being recognized in many organisms. In Brachionus rotifers, many morphologically similar yet genetically distinct species/biotypes have been described. A number of Brachionus cryptic species have been recognized among hatchery strains. In this study, we present a simple, one-step genetic method to detect the presence of those Brachionus sp. rotifers that have been ...
DOI:
10.1007/s10126-008-9119-4
PubMed:
18592313

2. A New Prokaryotic Farnesyldiphosphate Synthase from the Octocoral Eunicea Fusca: Differential Display, Inverse PCR, Cloning, and Characterization

Author:
Ranzer, Llanie K.; Brück, Thomas B.; Brück, Wolfram M.; Lopez, Jose V.; Kerr, Russell G.
Source:
Marine biotechnology 2009 v.11 no.1 pp. 62-73
ISSN:
1436-2228
Subject:
Symbiodinium; amino acid sequences; biosynthesis; corals; messenger RNA; polymerase chain reaction; salicylic acid; sequence homology; symbionts
Abstract:
... We recently reported that the biosynthesis of fuscol, a diterpene from the octocoral Eunicea fusca, is inducible by the application of plant signaling factors such as salicylic acid to the coral's algal symbiont. In this study, an mRNA differential display approach has been employed with the dinoflagellate symbiont of this octocoral which has led to the isolation of a farnesyldiphosphate synthase ...
DOI:
10.1007/s10126-008-9120-y

3. Alkaline Protease Gene Cloning from the Marine Yeast Aureobasidium pullulans HN2-3 and the Protease Surface Display on Yarrowia lipolytica for Bioactive Peptide Production

Author:
Ni, Xiumei; Yue, Lixi; Chi, Zhenming; Li, Jing; Wang, Xianghong; Madzak, Catherine
Source:
Marine biotechnology 2009 v.11 no.1 pp. 81-89
ISSN:
1436-2228
Subject:
Aureobasidium pullulans; Yarrowia lipolytica; antioxidant activity; complementary DNA; dairy protein; genes; molecular cloning; molecular weight; open reading frames; peptides; peptidyl-dipeptidase A; proteinases; single cell protein; yeasts
Abstract:
... The alkaline protease genes (cDNAALP2 gene and ALP2 gene) were amplified from complementary DNA (cDNA) and genomic DNA of the marine yeast Aureobasidium pullulans HN2-3, respectively. An open reading frame of 1,248 bp encoding a 415-amino acid protein with a calculated molecular weight of 42.9 kDa was characterized. The ALP2 gene contained two introns, which had 54 and 52 bp, respectively. When th ...
DOI:
10.1007/s10126-008-9122-9
PubMed:
18629587

4. Biogenic Origin of Polymetallic Nodules from the Clarion-Clipperton Zone in the Eastern Pacific Ocean: Electron Microscopic and EDX Evidence

Author:
Wang, Xiaohong; Schloßmacher, Ute; Wiens, Matthias; Schröder, Heinz C.; Müller, Werner E. G.
Source:
Marine biotechnology 2009 v.11 no.1 pp. 99-108
ISSN:
1436-2228
Subject:
bacteria; cobalt; copper; energy-dispersive X-ray analysis; iron; manganese; minerals; nickel; scanning electron microscopy; silicon; Pacific Ocean
Abstract:
... Polymetallic/ferromanganese nodules (Mn-nodules) have been assigned a huge economic potential since they contain considerable concentrations of manganese, copper, nickel, iron, and cobalt. It has been assumed that they are formed by, besides hydrogenous, nonbiogenic processes, biogenic processes based on metabolic processes driven by microorganisms. In the present study, we applied the techniques ...
DOI:
10.1007/s10126-008-9124-7
PubMed:
18670811

5. Characterization of Antifungal Chitinase from Marine Streptomyces sp. DA11 Associated with South China Sea Sponge Craniella Australiensis

Author:
Han, Yue; Yang, Bingjie; Zhang, Fengli; Miao, Xiaoling; Li, Zhiyong
Source:
Marine biotechnology 2009 v.11 no.1 pp. 132-140
ISSN:
1436-2228
Subject:
Aspergillus niger; Candida albicans; DNA; EDTA (chelating agent); Streptomyces peucetius; amino acid composition; amino acid sequences; ammonium sulfate; anion exchange chromatography; antifungal properties; barium; binding capacity; chitin; chitinase; copper; ethylene glycol tetraacetic acid; iron; magnesium; manganese; molecular cloning; molecular weight; nucleotide sequences; pH; polyacrylamide gel electrophoresis; salinity; sodium dodecyl sulfate; symbionts; temperature; urea; South China Sea
Abstract:
... The gene cloning, purification, properties, kinetics, and antifungal activity of chitinase from marine Streptomyces sp. DA11 associated with South China sponge Craniella australiensis were investigated. Alignment analysis of the amino acid sequence deduced from the cloned conserved 451 bp DNA sequence shows the chitinase belongs to ChiC type with 80% similarity to chitinase C precursor from Strept ...
DOI:
10.1007/s10126-008-9126-5
PubMed:
18626709

6. Immunomodulatory Effects of Polar Lichens on the Function of Macrophages In Vitro

Author:
Choi, Hye-Sook; Yim, Joung Han; Lee, Hong Kum; Pyo, Suhkneung
Source:
Marine biotechnology 2009 v.11 no.1 pp. 90-98
ISSN:
1436-2228
Subject:
Caloplaca; antibodies; drugs; gel electrophoresis; immunomodulation; interferon-gamma; interleukin-1; lichens; macrophage activation; macrophages; methanol; mice; mitogen-activated protein kinase; necrosis; nitric oxide; reactive oxygen species; transcription factor NF-kappa B; tumor necrosis factor-alpha; Antarctica
Abstract:
... Lichen species were collected from King George Island (Antarctica) and were screened for their immunomodulatory effect. Among the lichens tested, the methanol extract (CR-ME) of Caloplaca regalis showed the highest nitric oxide (NO) production in murine peritoneal macrophages. Therefore, this study further examined the ability of C. regalis to induce secretory and cellular responses in macrophages ...
DOI:
10.1007/s10126-008-9121-x
PubMed:
18612687

7. Preliminary Study on High-level Expression of Tandem-Arranged Tachyplesin-Encoding Gene in Bacillus subtilis Wb800 and its Antibacterial Activity

Author:
Dai, Jian-guo; Xie, Hai-wei; Jin, Gang; Wang, Wei-guang; Zhang, Yan; Guo, Yong
Source:
Marine biotechnology 2009 v.11 no.1 pp. 109-117
ISSN:
1436-2228
Subject:
Bacillus subtilis; Escherichia coli; Salmonella Typhimurium; annealing; antibacterial properties; antimicrobial peptides; cellulose; complementary DNA; fermentation; genes; genetic engineering; genetic vectors; nucleotide sequences; scanning electron microscopes; sucrose; transmission electron microscopes; transmission electron microscopy
Abstract:
... To produce tachyplesin, an antimicrobial peptide, by a stable and efficient gene engineering approach, cDNAs containing single tachyplesin gene sequence (tac)¹ and tandem repeat of tachyplesin gene sequence (tac2) were respectively developed by annealing two synthesized complementary single-stranded DNAs and constructed into pSBPTQ shuttle vector under the control of the SacB.p.s promoter. The vec ...
DOI:
10.1007/s10126-008-9125-6
PubMed:
18670810

8. A Fatty Acid from the Diatom Phaeodactylum tricornutum is Antibacterial Against Diverse Bacteria Including Multi-resistant Staphylococcus aureus (MRSA)

Author:
Desbois, Andrew P.; Mearns-Spragg, Andrew; Smith, Valerie J.
Source:
Marine biotechnology 2009 v.11 no.1 pp. 45-52
ISSN:
1436-2228
Subject:
Gram-negative bacteria; Phaeodactylum tricornutum; Staphylococcus aureus; antibacterial properties; antibiotics; bacterial infections; eicosapentaenoic acid; health services; mass spectrometry; microalgae; multiple drug resistance; reversed-phase high performance liquid chromatography; topical application
Abstract:
... Pathogenic bacteria, such as multidrug-resistant Staphylococcus aureus (MRSA), which are not susceptible to most conventional antibiotics, are causing increased concern in healthcare institutions worldwide. The discovery of novel antibacterial compounds for biomedical exploitation is one avenue that is being pursued to combat these problematic bacteria. Marine eukaryotic microalgae are known to pr ...
DOI:
10.1007/s10126-008-9118-5
PubMed:
18575935

9. Application of Nucleic-acid-based Therapeutics for Viral Infections in Shrimp Aquaculture

Author:
Shekhar, Mudagandur S.; Lu, Yuanan
Source:
Marine biotechnology 2009 v.11 no.1 pp. 1-9
ISSN:
1436-2228
Subject:
Baculoviridae; Gill-associated virus; Protoparvovirus; RNA interference; Taura syndrome virus; White spot syndrome virus; adaptive immunity; disease prevention; double-stranded RNA; eukaryotic cells; gene transfer; genes; nucleotides; pathogens; shellfish diseases; shrimp; shrimp culture; small interfering RNA; technology transfer; therapeutics; viruses
Abstract:
... Viral infections are one of the major reasons for the huge economic losses in shrimp farming. The control of viral diseases in shrimp remains a serious challenge for the shrimp aquacultural industry, with major pathogens, such as the white spot syndrome virus, yellow head virus, Taura syndrome virus, hepatopancreatic parvovirus, and baculoviruses, being geographically widespread. In the absence of ...
DOI:
10.1007/s10126-008-9155-0
PubMed:
18941835

10. Characterization of a Deep-Sea Sediment Metagenomic Clone that Produces Water-Soluble Melanin in Escherichia coli

Author:
Huang, Yali; Lai, Xintian; He, Xiaocui; Cao, Lixiang; Zeng, Zhirui; Zhang, Jiong; Zhou, Shining
Source:
Marine biotechnology 2009 v.11 no.1 pp. 124-131
ISSN:
1436-2228
Subject:
4-hydroxyphenylpyruvate dioxygenase; DNA; Escherichia coli; Idiomarina loihiensis; amino acids; bacteria; clones; genes; genomic libraries; marine sediments; melanin; metagenomics; microbial genetic resources; open reading frames; oxidation; physicochemical properties; sequence analysis
Abstract:
... To access to the microbial genetic resources of deep-sea sediment by a culture-independent approach, the sediment DNA was extracted and cloned into fosmid vector (pCC1FOS) generating a library of 39,600 clones with inserts of 24-45 kb. The clone fss6 producing red-brown pigment was isolated and characterized. The pigment was identified as melanin according to its physico-chemical characteristics. ...
DOI:
10.1007/s10126-008-9128-3
PubMed:
18648877

11. Cloning and Characterization of Alginate Lyase from a Marine Bacterium Streptomyces sp. ALG-5

Author:
Kim, Dong Eun; Lee, Eun Yeol; Kim, Hee Sook
Source:
Marine biotechnology 2009 v.11 no.1 pp. 10-16
ISSN:
1436-2228
Subject:
macroalgae; ionization; alginate lyase; polymerase chain reaction; Streptomyces; gel chromatography; genes; nucleotide sequences; affinity chromatography; mass spectrometry; bacteria
Abstract:
... A marine bacterium was isolated from seaweeds for its ability to degrade alginate. Analysis of 16S ribosomal DNA sequence and chemotaxonomic characterizations revealed that the strain belongs to Streptomyces. The alginate lyase gene of Streptomyces sp. ALG-5 was cloned by using PCR with the specific primer designed from homologous nucleotide sequences. The consensus sequences of N-terminal YXRSELR ...
DOI:
10.1007/s10126-008-9114-9
PubMed:
18553118

12. Identification of a Novel C20-elongase Gene from the Marine Microalgae Pavlova viridis and its Expression in Escherichia coli

Author:
Niu, Yan; Kong, Jian; Fu, Longyun; Yang, Jing; Xu, Yi
Source:
Marine biotechnology 2009 v.11 no.1 pp. 17-23
ISSN:
1436-2228
Subject:
Escherichia coli; Pavlova lutheri; amino acids; cell membranes; complementary DNA; docosahexaenoic acid; docosapentaenoic acid; eicosapentaenoic acid; endoplasmic reticulum; genes; green fluorescent protein; microalgae; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction
Abstract:
... Pavlova viridis, a species of a unicellular marine microalgae, is rich in the very-long-chain polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). A new elongase gene (elkj), with high identity with a functionally characterized C20-elongase of Pavlova lutheri, was isolated via reverse transcriptase-polymerase chain reaction using the pr ...
DOI:
10.1007/s10126-008-9116-7
PubMed:
18651191

13. Roles of Calmodulin and Calcium/Calmodulin-Dependent Protein Kinase in Flagellar Motility Regulation in the Coral Acropora Digitifera

Author:
Morita, Masaya; Iguchi, Akira; Takemura, Akihiro
Source:
Marine biotechnology 2009 v.11 no.1 pp. 118-123
ISSN:
1436-2228
Subject:
Acropora; ammonium chloride; antagonists; calcium; calmodulin; corals; eggs; pH; sperm motility; spermatozoa
Abstract:
... In the corals Acropora spp., eggs secrete substances that induce sperm motility regulation. An elevation of intracellular pH ([pH]i) and a regulation of intracellular Ca²⁺ concentration ([Ca²⁺]) are involved in the sperm motility regulation cascade. However, the detailed molecular aspects of flagellar motility regulation have not been fully demonstrated in Acropora. In this study, we determined th ...
DOI:
10.1007/s10126-008-9127-4
PubMed:
18661183