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- Zhang, Baohua; Skory, Christopher D.; Yang, Shang-Tian
- Metabolic Engineering 2012 v.14 no.5 pp. 512
- Rhizopus oryzae; biosynthesis; byproducts; carbon; ethanol; fermentation; fumaric acid; gene overexpression; genes; glucose; metabolic engineering; oxygen; pellets; phosphoenolpyruvate carboxylase; pyruvate carboxylase; resins
- ... Fumaric acid,a dicarboxylic acid used as a food acidulant and in manufacturing synthetic resins, can beproduced from glucose in fermentation by Rhizopus oryzae. However, the fumaric acid yield is limited by the co-production of ethanol and other byproducts. To increase fumaric acid production, over-expressing endogenous pyruvate carboxylase (PYC) and exogenous phosphoenolpyruvate carboxylase (PEPC ...