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- Montiel-Gonzalez, Maria Fernanda; Diaz Quiroz, Juan Felipe; Rosenthal, Joshua J.C.
- Methods 2019 v.156 pp. 16-24
- RNA editing; active sites; adenosine deaminase; amino acid sequences; antisense RNA; catalytic activity; gene editing; inosine; messenger RNA; mutation; nucleases; oligonucleotides; therapeutics
- ... Adenosine Deaminases that Act on RNA (ADARs) are a group of enzymes that catalyze the conversion of adenosines (A’s) to inosines (I’s) in a process known as RNA editing. Though ADARs can act on different types of RNA, editing events in coding regions of mRNA are of particular interest as I’s base pair like guanosines (G’s). Thus, every A-to-I change catalyzed by ADAR is read as an A-to-G change du ...
- Jinnah, Hussain; Ulbricht, Randi J.
- Methods 2019 v.156 pp. 40-45
- RNA editing; adenosine; amino acid substitution; animal models; deamination; gene editing; genetic variation; high-throughput nucleotide sequencing; inosine; messenger RNA; mice
- ... The deamination of adenosine to inosine by RNA editing is a widespread post-transcriptional process that expands genetic diversity. Selective substitution of inosine for adenosine in pre-mRNA transcripts can alter splicing, mRNA stability, and the amino acid sequence of the encoded protein. The functional consequences of RNA editing-dependent amino acid substitution are known for only a handful of ...