Jump to Main Content
- Cardenas-Garcia, Stivalis; Afonso, Claudio L.
- Methods in Molecular Biology 2017 v.1602 pp. 141-158
- DNA-directed RNA polymerase; Newcastle disease virus; Vaccinia virus; bacteriophages; complementary DNA; gene deletion; gene therapy; genes; molecular biology; plasmids; site-directed mutagenesis; vaccine development
- ... Reverse genetics allows for the generation of recombinant viruses or vectors used in functional studies, vaccine development, and gene therapy. This technique enables genetic manipulation and cloning of viral genomes, gene mutation through site-directed mutagenesis, along with gene insertion or deletion, among other studies. An in vitro infection-based system including the highly attenuated vaccin ...