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You searched for: Journal Microbial pathogenesis Remove constraint Journal: Microbial pathogenesis Publication Year 2020 Remove constraint Publication Year: 2020 Source 2020 v.138 Remove constraint Source: 2020 v.138
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1. Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)

Author:
Vasiee, Alireza; Norouzi, Neda; Yazdi, Farideh Tabatabaee; Mortazavi, Seyed Ali; Sankian, Mojtaba; Mahmoudi, Mahmoud; Shahidi, Fakhri
Source:
Microbial pathogenesis 2020 v.138 pp. 103819
ISSN:
0882-4010
Subject:
Amaranthus retroflexus; Lactococcus lactis; allergens; computer software; databases; dextranase; disulfide bonds; hosts; hypersensitivity; immunotherapy; lactic acid bacteria; physicochemical properties; probiotics; recombinant proteins; secretion; signal peptide
Abstract:
... Prokaryotic systems have been considered the most affordable and simplest hosts which are being employed to express recombinant proteins such as allergens; nevertheless, without appropriate signal peptide (SP), these systems cannot be used for secretory proteins. Recently, a lot of effort has been put into assessing the potential of gram-positive strains such as lactic acid bacteria for new applic ...
DOI:
10.1016/j.micpath.2019.103819

2. Antimicrobial activities of peptide Cbf-K16 against drug-resistant Helicobacter pylori infection in vitro and in vivo

Author:
Jiang, Meiling; Ma, Lingman; Huang, Ya; Wu, Haomin; Dou, Jie; Zhou, Changlin
Source:
Microbial pathogenesis 2020 v.138 pp. 103847
ISSN:
0882-4010
Subject:
DNA; Helicobacter pylori; adhesion; anti-inflammatory activity; antibiotic resistance; antimicrobial peptides; antimicrobial properties; bacteria; clarithromycin; epithelial cells; gastric mucosa; gastritis; gene expression; gene expression regulation; genes; interleukin-8; mechanism of action; mice; models; neutralization; plate count; proton pump inhibitors; secretion; therapeutics; virulence
Abstract:
... Helicobacter pylori (H. pylori) infection is highly prevalent, and has developed antimicrobial resistance to virtually all existing antibiotics. Currently, treatment of H. pylori infection (involving proton pump inhibitors and broad-spectrum antibiotics) is suboptimal, with high failure rates. Thus, there is a pressing need to develop new anti-H. pylori therapies. Cbf-K₁₆, a cathelicidin-like anti ...
DOI:
10.1016/j.micpath.2019.103847

3. Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6

Author:
Ohadi, Mandana; Forootanfar, Hamid; Dehghannoudeh, Gholamreza; Eslaminejad, Touba; Ameri, Atefeh; Shakibaie, Mojtaba; Adeli-Sardou, Mahboubeh
Source:
Microbial pathogenesis 2020 v.138 pp. 103806
ISSN:
0882-4010
Subject:
Acinetobacter junii; Candida utilis; Gram-negative bacteria; Proteus mirabilis; Pseudomonas aeruginosa; Staphylococcus aureus; antibacterial properties; biofilm; biosurfactants; cell lines; cytotoxicity; fungi; inhibitory concentration 50; lipopeptides; medicine; micelles; minimum inhibitory concentration
Abstract:
... Lipopeptide biosurfactants (LPBs) are amphiphilic compounds produced by microorganisms exhibiting various biological activities. The main aim of the present study was to assess the in vitro antimicrobial, anti-biofilm, and cytotoxic effects of LPB produced by Acinetobacter junii (AjL). We determined AjL minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria as ...
DOI:
10.1016/j.micpath.2019.103806

4. Anti-quorum sensing and antibiofilm activities of Blastobotrys parvus PPR3 against Pseudomonas aeruginosa PAO1

Author:
Parasuraman, Paramanantham; Devadatha, B.; Sarma, V. Venkateswara; Ranganathan, Sampathkumar; Ampasala, Dinakara Rao; Siddhardha, Busi
Source:
Microbial pathogenesis 2020 v.138 pp. 103811
ISSN:
0882-4010
Subject:
Avicennia marina; Blastobotrys; Chromobacterium violaceum; Pseudomonas aeruginosa; alginates; antimicrobial properties; bacteria; bacterial infections; bacterial motility; bioactive compounds; biodiversity; biofilm; biosensors; cell communication; chitinase; communications technology; computer simulation; elastase; ethyl acetate; exopolysaccharides; fungi; gas chromatography-mass spectrometry; internal transcribed spacers; marine ecosystems; marine environment; metabolites; pyocyanin; quorum sensing; receptors; screening; swimming; virulence; wood
Abstract:
... The bacterial cell communication also termed as Quorum sensing (QS) system was involved in the expression of several virulence traits during Pseudomonas infection. The attenuating of this bacterial cell communication system is an attractive approach for the management of bacterial infections without the complication of resistance development. In this respect, the marine environment has gained sign ...
DOI:
10.1016/j.micpath.2019.103811

5. Anti quorum sensing and anti virulence activity of tannic acid and it's potential to breach resistance in Salmonella enterica Typhi / Paratyphi A clinical isolates

Author:
Sivasankar, Chandran; Jha, Nisha Kumari; Ghosh, Ruchira; Shetty, Prathapkumar Halady
Source:
Microbial pathogenesis 2020 v.138 pp. 103813
ISSN:
0882-4010
Subject:
Fourier transform infrared spectroscopy; Salmonella enterica; amikacin; ampicillin; antibiotic resistance; azithromycin; bacterial motility; blood; chloramphenicol; ciprofloxacin; food pathogens; gentamicin; humans; hydrophobicity; peptides; phytochemicals; quorum sensing; screening; surfactants; tannins; therapeutics; typhoid fever; virulence
Abstract:
... Salmonella enterica Typhi and Paratyphi A are food borne pathogens causing typhoid, which is one of the most important food borne disease in the developing world. S. Typhi and S. Paratyphi A are of much concern as multi drug resistance has been on the rise. The current study is aimed to screen phytochemicals for anti quorum sensing (QS) activity against S. Typhi and S. Paratyphi A. Upon screening ...
DOI:
10.1016/j.micpath.2019.103813

6. A trivalent vaccine consisting of “flagellin A+B and pilin” protects against Pseudomonas aeruginosa infection in a murine burn model

Author:
Hashemi, Farhad B.; Behrouz, Bahador; Irajian, Gholamreza; Laghaei, Parisa; Korpi, Fatemeh; Fatemi, Mohammad Javad
Source:
Microbial pathogenesis 2020 v.138 pp. 103697
ISSN:
0882-4010
Subject:
Pseudomonas aeruginosa; animal diseases; antibiotic resistance; antibodies; antigens; burns (injuries); cross infection; fimbriae; flagellin; flagellum; interleukin-10; interleukin-12; lethal dose 50; mice; microbial load; models; pathogens; patients; protective effect; vaccination; vaccines
Abstract:
... Pseudomonas aeruginosa is a common nosocomial pathogen in burn patients, and rapidly achieves antibiotic resistance, and thus, developing an effective vaccine is critically important for combating P. aeruginosa infection. Flagella and pili play important roles in colonization of P. aeruginosa at the burn wound site and its subsequent dissemination to deeper tissue and organs. In the present study, ...
DOI:
10.1016/j.micpath.2019.103697

7. Biochanin A protect against lipopolysaccharide-induced acute lung injury in mice by regulating TLR4/NF-κB and PPAR-γ pathway

Author:
Hu, Xiansheng; Qin, Hongyu; Li, Yunpeng; Li, Jingxian; Fu, Lianjun; Li, Musen; Jiang, Cheng; Yun, Jinyan; Liu, Zhihu; Feng, Yao; Yao, Yuxuan; Yin, Baishuang
Source:
Microbial pathogenesis 2020 v.138 pp. 103846
ISSN:
0882-4010
Subject:
Toll-like receptor 4; anti-inflammatory activity; biochanin A; inflammation; interleukin-1beta; interleukin-6; lipopolysaccharides; lungs; mice; models; permeability; peroxisome proliferator-activated receptor gamma; protein content; signal transduction; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Acute lung injury (ALI) is a serious respiratory syndrome featured with uncontrolled inflammatory response. Biochanin A has been showed to possess and anti-inflammatory effect. This study intended to explore the suppression of biochanin A on lipopolysaccharide (LPS)-induced ALI in mice. Seven hours later LPS-induced ALI model established, the indexes including, pathological changes, MPO activity, ...
DOI:
10.1016/j.micpath.2019.103846

8. Biosynthesis of silver nanoparticles using red algae Portieria hornemannii and its antibacterial activity against fish pathogens

Author:
Fatima, Roohi; Priya, Monisha; Indurthi, Lavanya; Radhakrishnan, Vidya; Sudhakaran, Raja
Source:
Microbial pathogenesis 2020 v.138 pp. 103780
ISSN:
0882-4010
Subject:
Fourier transform infrared spectroscopy; Rhodophyta; Vibrio alginolyticus; Vibrio anguillarum; Vibrio parahaemolyticus; X-ray diffraction; absorbance; antibacterial properties; antibiotics; biosynthesis; color; energy-dispersive X-ray analysis; fish; fish diseases; moieties; nanoparticles; nanosilver; pathogens; scanning electron microscopy; silver; transmission electron microscopy; zeta potential
Abstract:
... In the present study, red algae Portieria hornemannii was scrutinized for the productive synthesis of silver nanoparticles. These biosynthesized nanoparticles were characterized by visible color change and ultraviolet visible spectrophotometry that indicated the formation of nanoparticles at the absorbance of 418 nm. Fourier transforms infrared spectroscopy, X-ray Diffraction analysis was further ...
DOI:
10.1016/j.micpath.2019.103780

9. Blocking cellular N-glycosylation suppresses human cytomegalovirus entry in human fibroblasts

Author:
Zheng, Luping; Li, Huifen; Fu, Li; Liu, Sally; Yan, Qiu; Leng, Sean X.
Source:
Microbial pathogenesis 2020 v.138 pp. 103776
ISSN:
0882-4010
Subject:
Human betaherpesvirus 5; epidermal growth factor; fibroblasts; flow cytometry; fluorescent antibody technique; glycoproteins; glycosylation; glycosyltransferases; humans; integrins; pathogenesis; peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase; receptors; therapeutics; tunicamycin
Abstract:
... N-glycosylation plays an important role in the pathogenesis of viral infections. However, the role of host cell N-glycosylation in human cytomegalovirus (hCMV) infection remains to be elucidated. In this study, we found that blocking or removal of cellular N-glycosylation by tunicamycin, peptide-N-glycosidase F (PNGase F) treatment, or N-acetylglucosaminyltransferase I (MGAT1) knockdown resulted i ...
DOI:
10.1016/j.micpath.2019.103776

10. Both gut microbiota and cytokines act to atherosclerosis in ApoE−/− mice

Author:
Liu, Qiuxia; Li, Yuchuan; Song, Xue; Wang, Jing; He, Zebao; Zhu, Jiansheng; Chen, Huazhong; Yuan, Jing; Zhang, Xue; Jiang, Haiyin; Zhang, Sheng; Ruan, Bing
Source:
Microbial pathogenesis 2020 v.138 pp. 103827
ISSN:
0882-4010
Subject:
Bacteroides; Ruminococcaceae; Verrucomicrobia; atherosclerosis; blood serum; chemokine CXCL5; cholesterol; digestive system; etiology; gene expression regulation; high density lipoprotein; high fat diet; intestinal microorganisms; low density lipoprotein; mice; protein synthesis; ribosomal DNA; sequence analysis; triacylglycerols
Abstract:
... Several studies have suggested a role for the gut microbiome and cytokines in atherosclerosis development, but combined analyses of the changes of the gut microbiota and cytokines have not been explored previously.We treated ApoE−/− and wild-type mice with a high-fat diet for 12 weeks. The gut microbiome and cytokine composition were analyzed using 16S ribosomal DNA sequencing and RayBio Quantibod ...
DOI:
10.1016/j.micpath.2019.103827

11. Branchial bioenergetics dysfunction as a relevant pathophysiological mechanism in freshwater silver catfish (Rhamdia quelen) experimentally infected with Flavobacterium columnare

Author:
Baldissera, Matheus D.; Souza, Carine F.; Dias, Juliane B.; Da Silva, Tatiane O.; Tavares, Guilherme C.; Valladão, Gustavo M.R.; da Silva, Aleksandro S.; Verdi, Camila Marina; Santos, Roberto C.V.; Vencato, Marina; da Veiga, Marcelo L.; da Rocha, Maria Izabel U.M.; Cunha, Mauro A.; Baldisserotto, Bernardo
Source:
Microbial pathogenesis 2020 v.138 pp. 103817
ISSN:
0882-4010
Subject:
Flavobacterium columnare; Rhamdia quelen; adenosine triphosphate; adenylate kinase; aquaculture industry; bacteria; columnaris disease; creatine kinase; discoloration; energy metabolism; enzyme activity; etiological agents; financial economics; freshwater; freshwater fish; gills; homeostasis; lipid peroxidation; mitochondria; mortality; necrosis; pathogenesis; pyruvate kinase; reactive oxygen species; signs and symptoms (animals and humans); sodium; sodium-potassium-exchanging ATPase
Abstract:
... Flavobacterium columnare, the causative agent of columnaris disease, is a serious bacterial disease responsible for causing devastating mortality rates in several species of freshwater fish, leading to severe economic losses in the aquaculture industry. Notwithstanding the enormous impacts this disease can have, very little is known regarding the interaction between the host and bacterium in terms ...
DOI:
10.1016/j.micpath.2019.103817

12. Changes in cardiac and hepatic energetic metabolism in gerbils infected by Listeria monocytogenes

Author:
Jaguezeski, Antonise M.; Souza, Carine F.; Perin, Géssica; Gebert, Roger R.; Baldi, Kelen R.A.; Gomes, Teane M.A.; Baldissera, Matheus D.; Andrade, Cinthia M.; Stefani, Lenita M.; Da Silva, Aleksandro S.
Source:
Microbial pathogenesis 2020 v.138 pp. 103786
ISSN:
0882-4010
Subject:
Listeria monocytogenes; adenosinetriphosphatase; antioxidant activity; creatine kinase; energy metabolism; enzyme activity; euthanasia; free radicals; gerbils; heart; histopathology; lipid peroxidation; listeriosis; liver; mitochondria; models; pyruvate kinase; sodium; sodium-potassium-exchanging ATPase
Abstract:
... Energy metabolism is a sensitive indicator of cellular disorders. Therefore, the objective of this study was to investigate changes in cardiac and hepatic energy metabolism during listeriosis using an experimental model. We divided gerbils into two groups: Control (n = 11) and orally Infected (n = 12) with 5 × 10⁹ CFU/mL of Listeria monocytogenes. Euthanasia and sampling were performed on days 6 a ...
DOI:
10.1016/j.micpath.2019.103786

13. Cloning, expression and nanodiscs assemble of recombinant HIV-1 gp41

Author:
Elbahnasawy, Mostafa A.; Farag, Mohamed M.S.; Mansour, Mohamed T.; El-Ghamery, Abbas A.
Source:
Microbial pathogenesis 2020 v.138 pp. 103824
ISSN:
0882-4010
Subject:
Escherichia coli; Human immunodeficiency virus 1; amino acids; cell membranes; epitopes; genetic vectors; hydrophobicity; lipids; membrane proteins; molecular weight; plasmids; protein synthesis; vaccine development
Abstract:
... Structural studies of membrane proteins have been hurdled by their difficulty for expression in heterogeneous expression systems due to their intrinsically strong hydrophobicity and requirements for association with other cellular membranes. This study aims to design a construct for expression of membrane proteins. Because of its outstanding interest in HIV-1 vaccine design, transmembrane gp41 ami ...
DOI:
10.1016/j.micpath.2019.103824

14. Cucumber mosaic virus infection modulated the phytochemical contents of Passiflora edulis

Author:
Lan, Hanhong; Lai, Baochun; Zhao, Peng; Dong, Xu; Wei, Wanting; Ye, Yanjie; Wu, Zujian
Source:
Microbial pathogenesis 2020 v.138 pp. 103828
ISSN:
0882-4010
Subject:
Cucumber mosaic virus; Passiflora edulis; control methods; enzyme activity; host plants; phytochemicals; plant diseases and disorders; plant viruses; polymerase chain reaction; secondary metabolites; sequence analysis; viruses
Abstract:
... Cucumber mosaic virus (CMV) caused huge agricultural impact on Passiflora edulis. However, the interactions between CMV and P. edulis are poorly unknown, which lead to lack of prevention and control measures. In this study, we identified the infection of CMV in P. edulis through modern small RNA sequencing (sRNA-seq) technology combined with traditional electron microscope and polymerase chain rea ...
DOI:
10.1016/j.micpath.2019.103828

15. Differential expression of structural and functional proteins during bean common mosaic virus-host plant interaction

Author:
Kumar, Sunil; Dhembla, Chetna; P, Hariprasad; Sundd, Monica; Patel, Ashok Kumar
Source:
Microbial pathogenesis 2020 v.138 pp. 103812
ISSN:
0882-4010
Subject:
Bean common mosaic virus; Phaseolus vulgaris; abiotic stress; beans; biosynthesis; biotic stress; carbon metabolism; chlorophyll; disease resistance; energy; gene expression regulation; host plants; host-pathogen relationships; leaves; photosynthesis; plant diseases and disorders; plant viruses; polyunsaturated fatty acids; protein synthesis; proteins; proteome; proteomics; two-dimensional gel electrophoresis
Abstract:
... Bean common mosaic virus (BCMV), the most common seed-borne pathogen in Phaseolus vulgaris L. is known to cause severe loss in productivity across the globe. In the present study, proteomic analyses were performed for leaf samples from control (healthy) and susceptible BCMV infected plants. The differential expression of proteins was evaluated using two-dimensional gel electrophoresis (2-DE). Appr ...
DOI:
10.1016/j.micpath.2019.103812

16. Disperse red 15 (DR15) impedes biofilm formation of uropathogenic Escherichia coli

Author:
Miryala, Sandeep; Makala, Himesh; Yadavali, Siva Prasad; Venkatasubramanian, Ulaganathan; Subbaiah, Nagarajan; Srinandan, C.S.
Source:
Microbial pathogenesis 2020 v.138 pp. 103772
ISSN:
0882-4010
Subject:
adhesins; adhesion; bacterial motility; benzoic acid; biofilm; catheters; coatings; cross infection; films (materials); fimbriae; health services; linseed oil; pathogenesis; plankton; quinones; urinary tract diseases; urine; uropathogenic Escherichia coli
Abstract:
... Catheter associated urinary tract infection (CAUTI) is a highly prevalent hospital-acquired infection that is predominantly caused by uropathogenic Escherichia coli (UPEC). It adheres on catheter surface using type I pili as the initial step of pathogenesis that progresses to form biofilm. In this study, potential inhibitors against FimH adhesin of type I pili were screened computationally that yi ...
DOI:
10.1016/j.micpath.2019.103772

17. Duckling short beak and dwarfism syndrome virus infection activates host innate immune response involving both DNA and RNA sensors

Author:
Chen, Shilong; Fang, Tiehui; Xiao, Shifeng; Lin, Fengqiang; Cheng, Xiaoxia; Wang, Shao; Zhu, Xiaoli; Chen, Xiuqin; Zheng, Min; Munir, Muhammad; Huang, Meiqing; Yu, Fusong; Chen, Shaoying
Source:
Microbial pathogenesis 2020 v.138 pp. 103816
ISSN:
0882-4010
Subject:
Anseriform dependoparvovirus 1; Toll-like receptor 1; Toll-like receptor 2; Toll-like receptor 3; Toll-like receptor 4; Toll-like receptor 5; Toll-like receptor 7; beak; bird diseases; double-stranded RNA; ducklings; dwarfing; fibroblasts; gene expression; gene expression regulation; genes; genomics; immune response; industry; innate immunity; interferon-beta; messenger RNA; pathogen-associated molecular patterns; pathogenesis; single-stranded DNA; viruses; waterfowl
Abstract:
... Duckling short beak and dwarfism syndrome virus (SBDSV), a newly identified goose parvovirus, causes devastating disease in domestic waterfowl and considerable economic losses to Chinese waterfowl industry. The molecular pathogenesis of SBDSV infection, nature and dynamics of host immune responses against SBDSV infection remained elusive. In this study, we systematically explored the relative mRNA ...
DOI:
10.1016/j.micpath.2019.103816

18. Effects of probiotics on cecal microbiome profile altered by duck Escherichia coli 17 infection in Cherry Valley ducks

Author:
Shi, Shuiqin; Qi, Zhao; Jiang, Wen; Quan, Suopei; Sheng, Tingting; Tu, Jian; Shao, Yin; Qi, Kezong
Source:
Microbial pathogenesis 2020 v.138 pp. 103849
ISSN:
0882-4010
Subject:
Escherichia coli; Lactobacillus casei; Lactobacillus rhamnosus; bird diseases; ducks; dysbiosis; genes; intestinal microorganisms; intestines; lactic acid bacteria; microbiome; pathogens; poultry industry; probiotics; ribosomal RNA; sequence analysis
Abstract:
... Avian colibacillosis is one of the most serious infectious bacterial diseases that endanger the modern poultry industry. Lactobacillus is believed to inhibit intestinal pathogens and maintain a healthy gut microbiota. This study aimed to investigate Lactobacillus supplementation in Cherry Valley ducks to prevent the intestinal flora dysbiosis caused by Duck Escherichia coli 17. One hundred and twe ...
DOI:
10.1016/j.micpath.2019.103849

19. Enhancement of host infectivity, immunity, and protective efficacy by addition of sodium bicarbonate antacid to oral vaccine formulation of live attenuated Salmonella secreting Brucella antigens

Author:
Hewawaduge, Chamith; Senevirathne, Amal; Lee, John Hwa
Source:
Microbial pathogenesis 2020 v.138 pp. 103857
ISSN:
0882-4010
Subject:
Brucella; CD4-positive T-lymphocytes; Salmonella Typhimurium; Western blotting; antigens; copper; edible vaccines; gene expression; genetic vectors; immune response; immunization; immunoglobulin G; immunohistochemistry; intestines; live vaccines; lymphocyte proliferation; mice; oral administration; outer membrane proteins; pathogenicity; plate count; protein secretion; sodium bicarbonate; superoxide dismutase
Abstract:
... In the present study, the importance of sodium bicarbonate antacid as an agent for an orally delivered attenuated Salmonella strain secreting Brucella antigens Cu–Zn superoxide dismutase (SodC) and outer membrane protein 19 (Omp19) as a live vaccine candidate against Brucella infection was investigated. First, Brucella antigens SodC and Omp19 were cloned into a prokaryotic constitutive expression ...
DOI:
10.1016/j.micpath.2019.103857

20. Establishment of a brain cell line obtained from hybrids of Channa argus ×Channa maculata for the detection of tilapia lake virus

Author:
Wang, Yingying; Li, Zhili; Wang, Qing; Zeng, Weiwei; Li, Yingying; Yin, Jiyuan; Bergmann, Sven M.; Zhu, Xinping
Source:
Microbial pathogenesis 2020 v.138 pp. 103810
ISSN:
0882-4010
Subject:
Channa argus; Tilapia lake virus; brain; cell lines; chromosome number; collagenase; cytogenetic analysis; electron microscopy; epithelium; females; fetal bovine serum; fluorescent antibody technique; hybrids; males; pathogenesis; trypsin; virus replication
Abstract:
... A brain cell line (CAMB) derived from hybrid snakehead (Channa argus (♂) × Channa maculata (♀)) was established by trypsin and collagenase combined digestion. The culturing conditions and cell biological characteristics were systematically studied. For growth of the cells, M199 medium containing 10% fetal bovine serum was used and at 27 °C incubated. Based on morphological analysis, CAMB cells wer ...
DOI:
10.1016/j.micpath.2019.103810