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- Author:
- Vasiee, Alireza; Norouzi, Neda; Yazdi, Farideh Tabatabaee; Mortazavi, Seyed Ali; Sankian, Mojtaba; Mahmoudi, Mahmoud; Shahidi, Fakhri
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- Amaranthus retroflexus; Lactococcus lactis; allergens; computer software; databases; dextranase; disulfide bonds; hosts; hypersensitivity; immunotherapy; lactic acid bacteria; physicochemical properties; probiotics; recombinant proteins; secretion; signal peptide
- Abstract:
- ... Prokaryotic systems have been considered the most affordable and simplest hosts which are being employed to express recombinant proteins such as allergens; nevertheless, without appropriate signal peptide (SP), these systems cannot be used for secretory proteins. Recently, a lot of effort has been put into assessing the potential of gram-positive strains such as lactic acid bacteria for new applic ...
- DOI:
- 10.1016/j.micpath.2019.103819
-
http://dx.doi.org/10.1016/j.micpath.2019.103819
- Author:
- Ohadi, Mandana; Forootanfar, Hamid; Dehghannoudeh, Gholamreza; Eslaminejad, Touba; Ameri, Atefeh; Shakibaie, Mojtaba; Adeli-Sardou, Mahboubeh
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- Acinetobacter junii; Candida utilis; Gram-negative bacteria; Proteus mirabilis; Pseudomonas aeruginosa; Staphylococcus aureus; antibacterial properties; biofilm; biosurfactants; cell lines; cytotoxicity; fungi; inhibitory concentration 50; lipopeptides; medicine; micelles; minimum inhibitory concentration
- Abstract:
- ... Lipopeptide biosurfactants (LPBs) are amphiphilic compounds produced by microorganisms exhibiting various biological activities. The main aim of the present study was to assess the in vitro antimicrobial, anti-biofilm, and cytotoxic effects of LPB produced by Acinetobacter junii (AjL). We determined AjL minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria as ...
- DOI:
- 10.1016/j.micpath.2019.103806
-
http://dx.doi.org/10.1016/j.micpath.2019.103806
- Author:
- Fu, Xiaozhe; Zhang, Lixi; Liu, Lihui; Lin, Qiang; Liang, Hongru; Niu, Yinjie; Huang, Zhibin; Li, Ningqiu
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- DNA; DNA viruses; annealing; cost effectiveness; detection; fish; inactivated vaccines; introns; messenger RNA; oligodeoxyribonucleotides; reverse transcriptase polymerase chain reaction; sequence analysis; temperature; transcription (genetics); transcriptomics; viruses
- Abstract:
- ... The virus inactivation test is a critical skill in inactivated vaccine production. Active viruses produced viral mRNA in susceptible cells or the host can be used to infer whether a DNA virus is replicating by RT-PCR. But it is generally difficult to avoid genomic DNA contamination in the samples. However, the use of primers spanning an intron is an effective alternative for virus inactivation tes ...
- DOI:
- 10.1016/j.micpath.2019.103822
-
http://dx.doi.org/10.1016/j.micpath.2019.103822
- Author:
- Zhang, Pengfei; Ye, Zhicang; Ye, Chen; Zou, Haijie; Gao, Zhichao; Pan, Jianyi
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- Escherichia coli; bacteria; environmental factors; immune response; infectious diseases; iron; mutants; outer membrane proteins; oxidative stress; pathogens; stress tolerance; transcription factors; virulence
- Abstract:
- ... Iron plays a central role at the interface of pathogen and host. The ability to sequester iron from a host not only reduces host immune defenses but also promotes pathogen virulence, leading to the occurrence of infectious disease. Recently, outer membrane protein OmpW was shown to protect bacteria against harsh environmental conditions and to play a role in infectious disease. The expression of t ...
- DOI:
- 10.1016/j.micpath.2019.103808
-
http://dx.doi.org/10.1016/j.micpath.2019.103808
- Author:
- Zhu, Wan; Chu, Yunzhuo; Zhang, Jingping; Xian, Wei; Xu, Xueying; Liu, Hongbo
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- Acinetobacter baumannii; cefoperazone; colistin; drugs; imipenem; induced resistance; monitoring; patients; pharmacodynamics; pharmacokinetics; probability; sulbactam; sulfonates; therapeutics; tigecycline; China
- Abstract:
- ... The aim of this study was to evaluate common antimicrobial regimens used in eradicating Acinetobacter baumannii in Shenyang, China.Monte Carlo simulation was conducted to estimate the probability target attainment (PTA) and cumulative fraction of response (CFR) for imipenem, cefoperazone/sulbactam (2:1), tigecycline and colistin methanesulfonate.For the results of PTAs, imipenem following administ ...
- DOI:
- 10.1016/j.micpath.2019.103809
-
http://dx.doi.org/10.1016/j.micpath.2019.103809
- Author:
- Hirudkar, Jayshri R.; Parmar, Komal M.; Prasad, Rupali S.; Sinha, Saurabh K.; Jogi, Mohit S.; Itankar, Prakash R.; Prasad, Satyendra K.
- Source:
- Microbial pathogenesis 2020 v.138
- ISSN:
- 0882-4010
- Subject:
- Psidium guajava; Shigella flexneri; antidiarrheal effect; antioxidant activity; antioxidants; biomarkers; blood composition; colon; computer simulation; crystal structure; diarrhea; electrolytes; enzyme activity; epithelium; gastrointestinal diseases; histopathology; interleukin-6; leaf extracts; leaves; medicinal plants; models; quercetin; rats; small intestine; sodium; sodium-potassium-exchanging ATPase; tumor necrosis factor-alpha; water content
- Abstract:
- ... The leaves of the plant Psidium guajava L. (Myrtaceae) has been traditionally used in treatment of various gastrointestinal disorders including diarrhoea and have also been reported for its potent antidiarrhoeal activity on various chemical induced diarrhoea models. The objective of our present study was to evaluate the potency of the leaf extract of the plant Psidium guajava (PGE) along with its ...
- DOI:
- 10.1016/j.micpath.2019.103807
-
http://dx.doi.org/10.1016/j.micpath.2019.103807