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You searched for: Journal Molecular and Cellular Endocrinology Remove constraint Journal: Molecular and Cellular Endocrinology Source 2015 v.405 Remove constraint Source: 2015 v.405 Subject cell culture Remove constraint Subject: cell culture Text Availability Full Text Remove constraint Text Availability: Full Text
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1. Production, purification, and characterization of recombinant hFSH glycoforms for functional studies

Author:
Butnev, Viktor Y.; Butnev, Vladimir Y.; May, Jeffrey V.; Shuai, Bin; Tran, Patrick; White, William K.; Brown, Alan; Smalter Hall, Aaron; Harvey, David J.; Bousfield, George R.
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 42-51
ISSN:
0303-7207
Subject:
Western blotting; binding sites; bioactive properties; cell culture; culture media; electrophoresis; females; follicle-stimulating hormone; glycosylation; humans; hydrophobicity; immunoaffinity chromatography; molecular weight
Abstract:
... Previously, our laboratory demonstrated the existence of a β-subunit glycosylation-deficient human FSH glycoform, hFSH21. A third variant, hFSH18, has recently been detected in FSH glycoforms isolated from purified pituitary hLH preparations. Human FSH21 abundance in individual female pituitaries progressively decreased with increasing age. Hypo-glycosylated glycoform preparations are significantl ...
DOI:
10.1016/j.mce.2015.01.026
PubMed:
25661536
PubMed Central:
PMC4378652