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You searched for: Journal name Molecular and cellular probes Remove constraint Journal name: Molecular and cellular probes Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019
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1. A duplex PCR assay for the simultaneous detection and differentiation of Muscovy duck parvovirus and goose parvovirus

Author:
Chunhe Wan; Longfei Cheng; Cuiteng Chen; Rongchang Liu; Shaohua Shi; Guanghua Fu; Hongmei Chen; Qiuling Fu; Yu Huang
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
Anseriform dependoparvovirus 1; Cairina moschata; Escherichia coli; Muscovy; detection limit; ducklings; genes; morbidity; mortality; nucleic acids; pathogens; polymerase chain reaction; vaccination; vaccines; waterfowl
Abstract:
... Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific primers after genetic comparison. Only three primers (GF1, MF1 and MGR1) were designe ...
DOI:
10.1016/j.mcp.2019.101439

2. A novel fluorometric method for inorganic pyrophosphatase detection based on G-quadruplex-thioflavin T

Author:
Han Zhao; Changbei Ma; Mingjian Chen
Source:
Molecular and cellular probes 2019 v.43 pp. 29-33
ISSN:
0890-8508
Subject:
copper; cost effectiveness; detection limit; enzyme activity; fluorescence; fluorometry; hydrolysis; inorganic pyrophosphatase; phosphates; sodium fluoride
Abstract:
... In this paper, we propose a fluorometric approach for the highly sensitive detection of inorganic pyrophosphatase (PPase) based on G-quadruplex-thioflavin T (ThT). In the absence of PPase, Cu2+ can coordinate with pyrophosphate (PPi) to generate a Cu2+/PPi complex. Then the G-rich sequence folds into the G-quadruplex structure, which can combine with ThT to generate a remarkable fluorescent signal ...
DOI:
10.1016/j.mcp.2018.12.003

3. Application of loop-mediated isothermal amplification (LAMP) for rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus) and haddock (Melanogrammus aeglefinus)

Author:
Yi Wang; Junli Feng; Xiaolan Tian
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
Gadus macrocephalus; Gadus morhua; Melanogrammus aeglefinus; cod (fish); detection limit; fluorescence; genes; haddock; loop-mediated isothermal amplification; marine fish; mitochondria; rapid methods; ribosomal DNA
Abstract:
... Codfish is a commercially important species of sea fish and plays an important role in the world fishery. In our study, two loop-mediated isothermal amplification (LAMP) assays (real-time fluorescence LAMP and visual LAMP) were established for the identification of three cod species in Gadidae (Gadus morhua, Gadus macrocephalus and Melanogrammus aeglefinus). 12S rDNA gene was used to design primer ...
DOI:
10.1016/j.mcp.2019.07.003

4. Assessment of ctDNA in CSF may be a more rapid means of assessing surgical outcomes than plasma ctDNA in glioblastoma

Author:
Jue-hui Li; Zhen-qiang He; Fu-hua Lin; Zheng-he Chen; Shi-yu Yang; Hao Duan; Xiao-bing Jiang; Fuad Al-Nahari; Xiang-heng Zhang; Jiang-huang Wang; Guan-hua Zhang; Zhen-feng Zhang; Cong Li; Yong-Gao Mou
Source:
Molecular and cellular probes 2019 v.46 pp. 101411
ISSN:
0890-8508
Subject:
DNA; biomarkers; blood; central nervous system; cerebrospinal fluid; exons; gene frequency; glioblastoma; high-throughput nucleotide sequencing; image analysis; magnetic materials; mutation; patients; surgery; tissues
Abstract:
... We aimed to develop a high-throughput deep DNA sequencing assay of cerebrospinal fluid (CSF) to identify clinically relevant oncogenic mutations that contribute to the development of glioblastoma (GBM) and serve as biomarkers to predict patients' responses to surgery. For this purpose, we recruited five patients diagnosed with highly suspicious GBM according to preoperative magnet resonance imagin ...
DOI:
10.1016/j.mcp.2019.06.001

5. Designing self-assembled peptide nanovaccine against Streptococcus pneumoniae: An in silico strategy

Author:
Hesam Dorosti; Mahboobeh Eslami; Navid Nezafat; Fardin Fadaei; Younes Ghasemi
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
CD4-positive T-lymphocytes; Streptococcus pneumoniae; antibiotics; biocompatibility; children; computer simulation; cytotoxic T-lymphocytes; diphtheria; elderly; immune response; immunodominant epitopes; immunogenicity; immunoinformatics; immunologic techniques; meningitis; nanoparticles; pathogens; peptides; pneumonia; polysaccharides; sepsis (infection); serotypes; toxoids; vaccine development; vaccines
Abstract:
... Streptococcus pneumoniae is the main cause of diseases such as meningitis, pneumoniae and sepsis, especially in children and old people. Due to costly antibiotic treatment, and increasing resistance of pneumococcus, developing high-efficient protective vaccine against this pathogen is an urgent need. Although the pneumoniae polysaccharide vaccine (PPV) and pneumonia conjugate vaccines (PCV) are th ...
DOI:
10.1016/j.mcp.2019.101446
PubMed:
31520715
PubMed Central:
PMC7126903

6. Detection of coralyne and heparin by polymerase extension reaction using SYBR Green I

Author:
Changbei Ma; Miangjian Chen; Hailun He; Leilei Chen
Source:
Molecular and cellular probes 2019 v.46 pp. 101423
ISSN:
0890-8508
Subject:
DNA; DNA probes; DNA-directed DNA polymerase; detection limit; drugs; fluorescence; heparin; nucleotide sequences; screening; therapeutics
Abstract:
... Polydeoxyadenosine (poly (dA)) has been extensively applied for detecting many drug molecules. Herein, we developed a sensitive method for detecting coralyne and heparin using a modified DNA probe with poly (dA) at one end. In the absence of coralyne, the DNA probe was digested by the Exonuclease I (Exo I), and therefore the SYBR Green I (SG I) emitted an extremely low fluorescent signal. While co ...
DOI:
10.1016/j.mcp.2019.101423

7. Development and evaluation of a direct TaqMan qPCR assay for the rapid detection of diverse carnivore amdoparvoviruses

Author:
Yan-Hong Wu; Tao Wei; Xiu-Ting Zhang; Yong-Qiang Zhao; Jian-Ke Wang; Li Cong; Bao-Zeng Xu; Xi-Qun Shao
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
Ailurus fulgens; Carnivore amdoparvovirus 1; DNA; Nyctereutes procyonoides; Vulpes lagopus; carnivores; detection limit; foxes; genes; mink; mink diseases; morbidity; mortality; pathogenesis; quantitative polymerase chain reaction; rapid methods; skunks
Abstract:
... Amdoparvoviruses infect carnivore species, including mink, raccoon dog, fox, skunk, and red panda. Amdoparvovirus infection is a major cause of morbidity and mortality in farmed minks. Here, we developed a direct TaqMan qPCR assay for detection and quantification of carnivore amdoparvoviruses by using three primers and one probe based on the conserved VP2 gene. The detection limit for Aleutian min ...
DOI:
10.1016/j.mcp.2019.101448

8. Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3

Author:
Yu Zhao; Hao-ying Han; Lin Fan; Run-Bo Tian; Jian-Tao Cui; Jing-Yi Li; Hong-Ying Chen; Ming-Fan Yang; Lan-Lan Zheng
Source:
Molecular and cellular probes 2019 v.45 pp. 31-36
ISSN:
0890-8508
Subject:
Porcine circovirus; Porcine circovirus-2; Porcine circovirus-3; detection limit; epidemiological studies; farms; fluorescence; livestock and meat industry; melting; mixed infection; molecular biology; pathogens; quantitative polymerase chain reaction; swine
Abstract:
... Porcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differentially detect PCV2 and PCV3. The assay specifically detected PCV2 and PCV3, wit ...
DOI:
10.1016/j.mcp.2019.04.001

9. Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood

Author:
Mark Jain; Alexander Vladimirovich Balatsky; Daria Borisovna Revina; Larisa Mikhailovna Samokhodskaya
Source:
Molecular and cellular probes 2019 v.43 pp. 13-19
ISSN:
0890-8508
Subject:
DNA; analytical kits; blood; blood sampling; laboratory equipment; markets; polymerase chain reaction; pregnancy; pregnant women; qualitative analysis; quantitative analysis; viruses
Abstract:
... Blood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isolation of cffDNA, which is a crucial step for success of subsequent analysis.To compare DSPVK and CNA ...
DOI:
10.1016/j.mcp.2018.12.006

10. Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay

Author:
Ruiwen Li; Jinfeng Wang; Libing Liu; Ruoxi Zhang; Xuepiao Hao; Qingan Han; Jianchang Wang; Wanzhe Yuan
Source:
Molecular and cellular probes 2019 v.45 pp. 14-18
ISSN:
0890-8508
Subject:
Actinobacillus pleuropneumoniae; DNA; diagnostic sensitivity; diagnostic specificity; etiological agents; genes; lungs; probability; quantitative polymerase chain reaction; swine; temperature; tonsils
Abstract:
... Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was developed to rapid detect A. pleuropneumoniae. Real-time RPA was performed successfully ...
DOI:
10.1016/j.mcp.2019.03.007

11. Effects of lycopene on ovarian cancer cell line SKOV3 in vitro: Suppressed proliferation and enhanced apoptosis

Author:
Jian Xu; Yuanfang Li; Hongyan Hu
Source:
Molecular and cellular probes 2019 v.46 pp. 101419
ISSN:
0890-8508
Subject:
Western blotting; apoptosis; cell lines; colorimetry; flow cytometry; lycopene; neoplasm cells; ovarian neoplasms; pro-apoptotic proteins; protein synthesis
Abstract:
... This study aims to investigate the influences of lycopene on ovarian cancer cells SKOV3 in vitro and the corresponding mechanism.SKOV3 cultures were divided into four groups and treated with lycopene at 0 (the control group), 1 × 10−6, 1 × 10−5 and 1 × 10−4 mol/L respectively. The proliferation rate of SKOV3 cells was determined using MTT colorimetric assay, and the apoptosis rate of SKOV3 cells w ...
DOI:
10.1016/j.mcp.2019.07.002

12. Fabry disease: Detection of Alu-mediated exon duplication by NGS

Author:
Martin Farr; Susana Ferreira; Arwa Al-Dilaimi; Sonja Bögeholz; Alexander Goesmann; Jörn Kalinowski; Cornelius Knabbe; Lothar Faber; João Paulo Oliveira; Volker Rudolph
Source:
Molecular and cellular probes 2019 v.45 pp. 79-83
ISSN:
0890-8508
Subject:
Fabry disease; copy number variation; exons; high-throughput nucleotide sequencing; mutation; polymerase chain reaction
Abstract:
... Monogenetic diseases can be analyzed routinely by targeted DNA sequencing. If causative variants are not found, complementary methods like RNA sequencing or analysis of copy number variations by multiplex ligation-dependent probe amplification have to be considered. In the latter, especially exonic duplications or deletions can be detected, but the precise sites of mutations remain unclear. As we ...
DOI:
10.1016/j.mcp.2019.03.008

13. GR and Foxa1 promote the transcription of ANGPTL4 in bovine adipocytes

Author:
Xue Mi; Yue Ning; Xiaoyu Wang; Zainaguli Junjvlieke; Li Wang; Shijie Wang; Weiyi Zhang; Xiaotong Su; Linsen Zan
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
RNA interference; adipocytes; adipose tissue; beef quality; cattle; enzyme activity; fatty acids; gel electrophoresis; gene expression; genes; homeostasis; lipolysis; lipoprotein lipase; liver; luciferase; messenger RNA; mutational analysis; oxidation; promoter regions; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transactivators; triacylglycerols
Abstract:
... ANGPTL4 (angiopoietin-like 4) is a secreted protein involved in triacylglycerol homeostasis. It is a key enzyme in lipolysis, which stimulates the oxidation of fatty acids and inhibits fat accumulation by inhibiting the activity of lipoprotein lipase (LPL). Using quantitative Real-Time PCR (qRT-PCR) to investigate the mRNA expression pattern of the bovine ANGPTL4 gene in different tissues and orga ...
DOI:
10.1016/j.mcp.2019.101443

14. MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1

Author:
Hui Liu; Zengfeng Ni; Lili Shi; Lijie Ma; Jianqiang Zhao
Source:
Molecular and cellular probes 2019 v.44 pp. 37-43
ISSN:
0890-8508
Subject:
Western blotting; apoptosis; caspase-3; leukemia; leukocytes; neoplasm cells; quantitative polymerase chain reaction; therapeutics
Abstract:
... Studies have reported that micro (miR)-486-5p plays a crucial part in the progression of leukemia, however, to the best of our knowledge, few studies have been conducted on its mechanism in leukemia. In this study, the mechanism of miR-486-5p in leukemia cells was pointed out and its possible target genes were analyzed for the purpose of providing new therapeutic strategies for treating leukemia p ...
DOI:
10.1016/j.mcp.2019.02.001

15. One-step multiplex real-time RT-PCR for detection and typing of dengue virus

Author:
Myung-Jin Mun; Joon-Yong Bae; Jin Hyuck Kim; Soo Bok Kim; Ilseob Lee; Jin Il Kim; Mee Sook Park; Man-Seong Park; Yong Suk Nam
Source:
Molecular and cellular probes 2019 v.43 pp. 86-91
ISSN:
0890-8508
Subject:
Dengue virus; dengue; diagnostic techniques; epidemiological studies; genotype; monitoring; patients; prognosis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; secondary infection; serotypes; viral load; viruses
Abstract:
... Previous studies reported that severity of dengue is associated with multiple factors, including secondary infection, age, viral load and infecting serotype and genotype. In addition, other studies have reported that a dengue virus-2 (DENV-2) infection is associated with a prognosis of more severe clinical manifestations than DENV-1 and DENV-4 infections. For these reasons, the ability to identify ...
DOI:
10.1016/j.mcp.2018.10.001

16. Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population

Author:
Malik Siddique Mahmood; Muhammad Asad-Ullah; Hina Batool; Sana Batool; Naeem Mehmood Ashraf
Source:
Molecular and cellular probes 2019 v.43 pp. 40-44
ISSN:
0890-8508
Subject:
Chlamydia; HLA antigens; Neisseria gonorrhoeae; alleles; allergenicity; antibiotic resistance; antibiotics; disease transmission; epitopes; immunoinformatics; pathogens; prediction; sexually transmitted diseases; vaccines; United States
Abstract:
... Gonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Although extensive ...
DOI:
10.1016/j.mcp.2018.11.007

17. Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification

Author:
Rong Zeng; Jinyan Luo; Shigang Gao; Lihui Xu; Zhiwei Song; Fuming Dai
Source:
Molecular and cellular probes 2019 v.43 pp. 84-85
ISSN:
0890-8508
Subject:
Cucumber green mottle mosaic virus; RNA; detection limit; leaves; rapid methods; reverse transcription
Abstract:
... Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves. ...
DOI:
10.1016/j.mcp.2018.12.005

18. The lipid profile of three Malassezia species assessed by Raman spectroscopy and discriminant analysis

Author:
Chrysoula Petrokilidou; Eleftherios Pavlou; Georgios Gaitanis; Ioannis D. Bassukas; Manolis N. Saridomichelakis; Aristea Velegraki; Nikolaos Kourkoumelis
Source:
Molecular and cellular probes 2019 v.46 pp. 101416
ISSN:
0890-8508
Subject:
Malassezia; Raman spectroscopy; agar; discriminant analysis; flora; least squares; lipid composition; lipids; lipophilicity; mass spectrometry; models; phylogeny; skin diseases; species identification; vertebrates; yeasts
Abstract:
... Malassezia yeasts constitute the major eukaryotic cutaneous flora of homoeothermic vertebrates. These lipophilic yeasts are able to cause, trigger, or aggravate common skin diseases under favorable conditions. Species identification and subspecies differentiation is currently based on morphological characteristics, lipid assimilation profile, and molecular tests. Mass spectrometry has been also re ...
DOI:
10.1016/j.mcp.2019.06.006

19. The ribosomal intergenic spacer (IGS) in the potato and tobacco cyst nematodes, Globodera pallida, G. rostochiensis and G. tabacum

Author:
Mehrdad Madani; Len Ward; Andy Vierstraete; Solke H. De Boer; Maurice Moens
Source:
Molecular and cellular probes 2019 pp. -
ISSN:
0890-8508
Subject:
Globodera pallida; Globodera rostochiensis; Globodera tabacum; cyst nematodes; diagnostic techniques; expressed sequence tags; intergenic DNA; molecular cloning; multigene family; polymerase chain reaction; potatoes; ribosomal DNA; ribosomal RNA; sequence analysis; tobacco
Abstract:
... The potato cyst nematodes Globodera pallida and G. rostochiensis (PCN), and tobacco cyst nematode (TCN), G. tabacum, are the most important parasitic nematodes of potato and tobacco worldwide. Ribosomal DNA provides useful molecular data for diagnostics, the study of polymorphisms and for evolutionary research in eukaryotic organisms including nematodes. Here we present data on the structure and o ...
DOI:
10.1016/j.mcp.2019.101441

20. Use of whole exome sequencing in the NICU: Case of an extremely low birth weight infant with syndromic features

Author:
Benjamin Kuehne; Eva Heine; Hormos Salimi Dafsari; Raphael Irwin; Raoul Heller; Ursula Bangen; Konrad Brockmeier; Angela Kribs; André Oberthuer; Sebahattin Cirak
Source:
Molecular and cellular probes 2019 v.45 pp. 89-93
ISSN:
0890-8508
Subject:
diagnostic techniques; genetic disorders; heart failure; heterozygosity; hyperglycemia; infants; low birth weight; morbidity; mortality; oncogenes; patients; phenotype; pregnancy; premature birth; rats; sarcoma; sequence analysis; tachycardia
Abstract:
... Single gene (Mendelian) disorders are one of the leading causes of neonatal morbidity and mortality. However, in the setting of preterm birth phenotypic features of genetic diseases are often undifferentiated and are clinically very difficult to interpret based on the wide range of differential diagnoses. We report an extremely low birth weight infant (ELBW) born prematurely at 23 + 0 gestational ...
DOI:
10.1016/j.mcp.2019.03.002