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You searched for: Journal name Molecular biology reports Remove constraint Journal name: Molecular biology reports Publication year rev 7978-2022 Remove constraint Publication year rev: 7978-2022 Source 2022 v.49 no.3 Remove constraint Source: 2022 v.49 no.3
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1. A molecular insight of inflammatory cascades in rheumatoid arthritis and anti-arthritic potential of phytoconstituents

Author:
Vaibhav Patidar; Shruti Shah; Rahul Kumar; Pankaj Kumar Singh; Shashi Bala Singh; Dharmendra Kumar Khatri
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2375-2391
ISSN:
0301-4851
Subject:
T-lymphocytes; apoptosis; aryl hydrocarbon receptors; cartilage; cell differentiation; chemical constituents of plants; fibroblasts; hyperplasia; molecular biology; nucleoproteins; osteoclasts; phytochemicals; rheumatoid arthritis
Abstract:
... Rheumatoid arthritis (RA) is an auto-immune inflammatory disorder of the synovial lining of joints marked by immune cells infiltration and hyperplasia of synovial fibroblasts which results in articular cartilage destruction and bone erosion. The current review will provide comprehensive information and results obtained from the recent research on the phytochemicals which were found to have potenti ...
DOI:
10.1007/s11033-021-06986-7

2. Anti-inflammatory cytokines in sickle cell disease

Author:
Adekunle E. Alagbe; Igor F. Domingos; Adekunle D. Adekile; Maria H. S. L. Blotta; Magnun N. N. Santos
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2433-2442
ISSN:
0301-4851
Subject:
inflammation; interleukin-10; interleukin-2; molecular biology; pathophysiology; sickle cell anemia; therapeutics
Abstract:
... Sickle cell disease (SCD) is a well-studied monogenetic disease with an established chronic inflammatory component. The paradigm shift towards inflammation has made the pathophysiology of SCD even more complex. Studies have shown that an imbalance between the pro-inflammatory and anti-inflammatory cytokines in SCD exists; however, the reports are skewed toward the pro-inflammatory mediators. We en ...
DOI:
10.1007/s11033-021-07009-1

3. Coenzyme Q10 reduces expression of apoptotic markers in adult rat nucleus accumbens dopaminergic neurons treated with methamphetamine

Author:
S. B. Jameie; A. Kazemian; Z. Sanadgol; S. Asadzadeh Bayqara; Mana Sadat Jameie; M. Farhadi
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2273-2281
ISSN:
0301-4851
Subject:
adults; apoptosis; caspase-3; clinical trials; coenzyme Q10; cognition; drug abuse; males; methamphetamines; molecular biology; neurons; neuroprotective effect; rats; tyrosine 3-monooxygenase
Abstract:
... BACKGROUND: Abuse of addictive drugs such as methamphetamine (METH) has become a global problem, leading to many social, economic, and health disturbances, including neurological and cognitive disorders. Neuronal damage is reported in chronic METH abusers. The neuroprotective role of CoQ10 has been shown in many studies. In the present study, we aimed to assess the pre and post-efficacy of CoQ10 o ...
DOI:
10.1007/s11033-021-07049-7

4. Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1

Author:
Hongli Xu; Shengnan Liang; Junjie Hu; Wentong Liu; Zhiqiang Dong; Shaozhong Wei
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1661-1668
ISSN:
0301-4851
Subject:
cell cycle; cell growth; colorectal neoplasms; cyclins; metastasis; molecular biology; mortality; neoplasm cells; therapeutics; transcription (genetics); transcription factors; ubiquitin-protein ligase
Abstract:
... BACKGROUND: The mortality rate of colorectal cancer (CRC) remains high in developing countries. Interventions that can inhibit the proliferation of tumor cells represent promising strategies in CRC treatment. Deltex E3 ubiquitin ligase 3 (DTX3) plays an essential role in tumor development and may predict the outcome of cancer patients. This study aimed to investigate the regulatory mechanisms of D ...
DOI:
10.1007/s11033-021-06916-7

5. Development and performance evaluation of TaqMan real-time fluorescence quantitative methylation specific PCR for detecting methylation level of PER2

Author:
Huihui Jiang; Xin Yang; Miaomiao Mi; Xiaonan Wei; Hongyuan Wu; Yu Xin; Liping Jiao; Shengjun Sun; Chengming Sun
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2097-2105
ISSN:
0301-4851
Subject:
DNA; bisulfites; detection limit; fluorescence; genes; high-throughput nucleotide sequencing; leukemia; methylation; molecular biology
Abstract:
... BACKGROUND: PER2 gene methylation is closely related to the occurrence and progress of some cancers, but there is no method to quantitatively detect PER2 methylation in conventional laboratories. So, we established a TaqMan real-time fluorescence quantitative methylation specific PCR (TaqMan real-time FQ-MSP) assay and use it for quantitative detection of PER2 methylation in leukemia patients. MET ...
DOI:
10.1007/s11033-021-07027-z

6. Effect of NMDA receptor agonist and antagonist on spermatogonial stem cells proliferation in 2- and 3- dimensional culture systems

Author:
Amirhessam Eskafi Noghani; Reza Asadpour; Adel Saberivand; Zohreh Mazaheri; Gholamreza Hamidian
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2197-2207
ISSN:
0301-4851
Subject:
agonists; antagonists; flow cytometry; gene expression; glutamic acid; immunocytochemistry; leukemia; males; mice; molecular biology; serine; testes; zinc finger motif
Abstract:
... BACKGROUND: The main purpose of this study was to investigate the effect of D-serine (DS) and Dizocilpine (MK-801) on the proliferation of spermatogonial stem cells (SSCs) in two-dimensional (2D) and three-dimensional (3D) culture systems. METHODS AND RESULTS: The SSCs of male NMRI mice were isolated by enzymatic digestion and cultured for two weeks. Then, the identity of SSCs was validated by ant ...
DOI:
10.1007/s11033-021-07041-1

7. Evaluating the effects of sodium glucose co-transporter -2 inhibitors from a renin–angiotensin–aldosterone system perspective in patients infected with COVID-19: contextualizing findings from the dapagliflozin in respiratory failure in patients with COVID-19 study

Author:
Diala Alhaj Moustafa; Zainab Imran; Roua Ismail; Menatallah Rayan; Alain-Pierre Gadeau; Hussein Eldassouki; Nabeel Abdulrahman; Fatima Mraiche
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2321-2324
ISSN:
0301-4851
Subject:
COVID-19 infection; glucose; molecular biology; noninsulin-dependent diabetes mellitus; sodium; sodium glucose cotransporter-2 inhibitors
Abstract:
... Numerous studies demonstrate parallels between CVD, type 2 diabetes mellitus (T2DM) and COVID-19 pathology, which accentuate pre-existing complications in patients infected with COVID-19 and potentially exacerbate the infection course. Antidiabetic drugs such as sodium-glucose transporter-2 (SGLT-2) inhibitors have garnered substantial attention recently due to their efficacy in reducing the sever ...
DOI:
10.1007/s11033-022-07183-w

8. Exogenous adenosine activates A2A adenosine receptor to inhibit RANKL-induced osteoclastogenesis via AP-1 pathway to facilitate bone repair

Author:
Xin Cheng; Chengcheng Yin; Yongqiang Deng; Zubing Li
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2003-2014
ISSN:
0301-4851
Subject:
RNA; Western blotting; adenosine; antagonists; antigens; bone formation; fluorescent antibody technique; homeostasis; ligands; molecular biology; nanofibers; osteoclasts; purinergic receptors; quantitative polymerase chain reaction; rats
Abstract:
... BACKGROUND: Adenosine is a purine nucleoside involved in regulating bone homeostasis through binding to A1, A2A, A2B, and A3 adenosine receptors (A1R, A2AR, A2BR, and A3R, respectively). However, the underlying mechanisms by which adenosine and receptor subtypes regulate osteoclast differentiation remain uncertain. This study aims to assess the role of exogenous adenosine and receptor subtypes in ...
DOI:
10.1007/s11033-021-07017-1

9. Exosomal miR-19a decreases insulin production by targeting Neurod1 in pancreatic cancer associated diabetes

Author:
Jiaojiao Su; Wenjing Pang; Aisen Zhang; Lei Li; Weiyan Yao; Xin Dai
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1711-1720
ISSN:
0301-4851
Subject:
Western blotting; calcium; cell communication; diabetes mellitus; exosomes; genes; glucose; insulin; insulin secretion; luciferase; microRNA; molecular biology; pancreatic neoplasms; pathogenesis; quantitative polymerase chain reaction
Abstract:
... BACKGROUND: New onset diabetes mellitus demonstrates a roughly correlation with pancreatic cancer (PaC), which is unique in PaC and was named as PaC-induced DM, but the inner mechanism remains unclear. Exosomes mediate intercellular communication and bearing microRNAs might be direct constituent of effect in target cells. METHODS AND RESULTS: The isolated exosomes from PaC cells were used to treat ...
DOI:
10.1007/s11033-021-06980-z

10. Gastrointestinal cancer drug resistance: the role of exosomal miRNAs

Author:
Mahsa Salehi; Asma Vafadar; Seyyed Hossein Khatami; Mortaza Taheri-Anganeh; Omid Vakili; Amir Savardashtaki; Babak Negahdari; Parisa Naeli; Hamid Behrouj; Hassan Ghasemi; Ahmad Movahedpour
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2421-2432
ISSN:
0301-4851
Subject:
angiogenesis; antineoplastic agents; carcinogenesis; cell communication; colon; drug resistance; endosomes; esophagus; exosomes; gastrointestinal neoplasms; liver; microRNA; molecular biology; pancreas; stomach; therapeutics
Abstract:
... Resistance of gastrointestinal (GI) cancer cells to therapeutic agents are one of the major problems in treating this type of cancer. Although the exact mechanism of drug resistance has not yet been fully elucidated, various factors have been identified as contributing factors involved in this process. Several studies have revealed the role of exosomes, especially exosomal microRNAs (miRNAs), in G ...
DOI:
10.1007/s11033-021-07007-3

11. High-fat diet intake ameliorates the expression of hedgehog signaling pathway in adult rat liver

Author:
Rabia Mehmood; Nadeem Sheikh; Muhammad Babar Khawar; Muddasir Hassan Abbasi; Maryam Mukhtar
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1985-1994
ISSN:
0301-4851
Subject:
Erinaceidae; Rattus norvegicus; adults; animal models; biomarkers; collagen; fatty liver; fibrosis; gene expression; hepatocytes; high fat diet; hypertriglyceridemia; inflammation; lipid composition; liver; liver cirrhosis; molecular biology; necrosis; neutropenia; polycythemia; rats; very low density lipoprotein
Abstract:
... BACKGROUND: Disproportionate fatty diet intake provokes hepatic lipid accumulation that causes non-alcoholic fatty liver disease, triggering the embryonically conserved Hedgehog (Hh) pathway in the adult liver. The present study incorporates exploring the impact of chronically administered unsaturated (D-1) and saturated (D-2) fat-enriched diets on hematological parameters, liver functioning, and ...
DOI:
10.1007/s11033-021-07012-6

12. Identification of putative genetic variants in major depressive disorder patients in Pakistan

Author:
Sarah Rizwan Qazi; Muhammad Irfan; Zoobia Ramzan; Muhammad Jahanzaib; Maleeha Zaman Khan; Mahrukh Nasir; Muhammad Shakeel; Ishtiaq Ahmad Khan
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2283-2292
ISSN:
0301-4851
Subject:
bioinformatics; gene frequency; genes; mental depression; molecular biology; pathogenesis; suicide; Pakistan
Abstract:
... BACKGROUND: Major depressive disorder (MDD) is a polygenic, and highly prevalent disorder affecting 322 million people globally. It results in several psychological changes which adversely affect different dimensions of life and may lead to suicide. METHODS: Whole exome sequencing of 15 MDD patients, enrolled at the Dr. A. Q. Khan Institute of Behavioral Sciences, Karachi, was performed using Next ...
DOI:
10.1007/s11033-021-07050-0

13. Increased expression of HMGB1 in the implantation phase endometrium is related to recurrent implantation failure

Author:
Mi Han; Yi Cao; Wenjie Zhou; Mingjuan Zhou; Xiaowei Zhou; Dan Zhang; Bufang Xu; Aijun Zhang
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1701-1710
ISSN:
0301-4851
Subject:
Western blotting; adhesion; blastocyst; cell adhesion; conception; embryo transfer; endometrium; epithelial cells; epithelium; humans; immunohistochemistry; molecular biology; nucleoproteins; reverse transcriptase polymerase chain reaction
Abstract:
... BACKGROUND: Impaired endometrial receptivity was the main cause of recurrent implantation failure (RIF); however, its underlying mechanisms had not been elucidated. This study aimed to determine the expression level of high-mobility group box protein 1 (HMGB1) in the endometrium with RIF and its effect on endometrial receptivity. METHODS AND RESULTS: Genome-wide expression profiling, real-time rev ...
DOI:
10.1007/s11033-021-06979-6

14. Investigation of VHL gene associated with miR-223 in clear cell renal cell carcinoma

Author:
Ufuk Unal; Gulsah Cecener; Havva Tezcan Unlu; Berna Aytac Vuruskan; Ecem Efendi Erdem; Unal Egeli; Hulya Ozturk Nazlioglu; Onur Kaygisiz; Berrin Tunca; Hakan Vuruskan
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2073-2083
ISSN:
0301-4851
Subject:
DNA; bioinformatics; biomarkers; gene expression; genes; molecular biology; mutational analysis; nucleotide sequences; prognosis; quantitative polymerase chain reaction; renal cell carcinoma; sequence analysis; t-test
Abstract:
... BACKGROUND: Clear cell type renal cell carcinoma (ccRCC) is the most common renal cell carcinoma (RCC). In this study, we examined the expressions of VHL and miR-223 in ccRCC patients׳ tissues to investigate the possible role in the development of ccRCC. METHODS AND RESULTS: This study collected five expression profiles (GSE36139, GSE3, GSE73731, GSE40435, and GSE26032) from Gene Omnibus Data. Exp ...
DOI:
10.1007/s11033-021-07025-1

15. Mitochondrial DNA and Alzheimer’s disease: a first case–control study of the Tunisian population

Author:
Nesrine Ben Salem; Sami Boussetta; Itziar de Rojas; Sonia Moreno-Grau; Laura Montrreal; Narjes Mokni; Imene Mahmoud; Samia Younes; Nizar Daouassi; Mahbouba Frih-Ayed; Afef Hammami; Amel Ben Ammar Elgaaied; Agustín Ruiz; Lotfi Cherni
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1687-1700
ISSN:
0301-4851
Subject:
apolipoprotein E; case-control studies; etiology; genotyping; mitochondria; mitochondrial DNA; molecular biology; neurodegenerative diseases; pathogenicity; risk factors
Abstract:
... BACKGROUND: Alzheimer’s disease (AD) is the most common neurodegenerative disorder in humans and presents a major health problem throughout the world. The etiology of AD is complex, and many factors are implicated, including mitochondria. Mitochondrial alteration has been proposed as a possible cause of AD. Therefore, several studies have focused on finding an association between inherited mitocho ...
DOI:
10.1007/s11033-021-06978-7

16. Notch pathway inhibition mediated by arsenic trioxide depletes tumor initiating cells in small cell lung cancer

Author:
Meng-Hang Yang; Bing Li; Ke-Jie Chang
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2245-2253
ISSN:
0301-4851
Subject:
arsenic oxide; cell proliferation; humans; lung neoplasms; molecular biology; stem cells; xenotransplantation
Abstract:
... BACKGROUND: Small cell lung cancer (SCLC) is the most malignant type of lung cancer. We previously reported that arsenic trioxide (As₂O₃) inhibited tumor initiating cells (TICs) of SCLC in vitro. In the present study, we aimed to identify the above effect in vivo and shed light on its underlying mechanism. METHODS AND RESULTS: TICs were enriched by culturing human SCLC cell line as sphere cells in ...
DOI:
10.1007/s11033-021-07046-w

17. Phenotyping and validation of molecular markers associated with rust resistance genes in wheat cultivars in Egypt

Author:
Adel A. Elshafei; Waled M. El-Orabey; Fathallah B. Fathallah; Ramadan M. Esmail; Mohamed A. Abou-Zeid
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1903-1915
ISSN:
0301-4851
Subject:
cultivars; disease progression; genetic markers; leaf rust; leaves; marker-assisted selection; mature plants; molecular biology; phenotype; resistance genes; stem rust; stripe rust; wheat; Egypt
Abstract:
... BACKGROUND: Thirteen Egyptian wheat cultivars were evaluated and characterized for adult plant resistance to yellow, leaf, and stem rusts. SSR markers linked to yellow, leaf and stem rust resistance genes were validated and subsequently used to identify wheat cultivars containing more than one rust resistance gene. RESULTS: Results of the molecular marker detection indicated that several genes, ei ...
DOI:
10.1007/s11033-021-07002-8

18. Phylogenetic relationship and genetic diversity of Turkish peanut viruses

Author:
Ali Karanfil
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2293-2301
ISSN:
0301-4851
Subject:
Arachis hypogaea; Bean common mosaic virus; Peanut mottle virus; genetic distance; genetic variation; genome; molecular biology; peanuts; phylogeny; sequence homology; turnips; viruses
Abstract:
... BACKGROUND: Viral diseases of peanuts (Arachis hypogaea) have never been reported in Turkey. Analyzing genetic diversity using phylogenetic analysis is important to understanding disease complexes. METHODS AND RESULTS: Samples were taken from 63 plants showing virus-like symptoms from two major cultivation regions in Turkey. These samples were tested against five different virus genera and one vir ...
DOI:
10.1007/s11033-022-07137-2

19. Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus)

Author:
Yue Song; Xiaoying Yang; Xueying Zhang; Jueyu Zhu; Yixin Chen; Fuli Gao; Haolin Zhang; Yingying Han; Qiang Weng; Zhengrong Yuan
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2209-2215
ISSN:
0301-4851
Subject:
Spermophilus; colon; cytoplasm; immunohistochemistry; molecular biology; quantitative polymerase chain reaction
Abstract:
... BACKGROUND: The purpose of the experiment was to explore the localization and seasonal expression of extracellular signal regulated kinase (ERK) in the colonic tissue of wild ground squirrels (Spermophilus dauricus). METHODS AND RESULTS: Hematoxylin–eosin staining, immunohistochemistry, real-time quantitative PCR and Western blotting were used in this experiment. The histological results showed th ...
DOI:
10.1007/s11033-021-07042-0

20. Serum-derived miR-574-5p-containing exosomes contribute to liver fibrosis by activating hepatic stellate cells

Author:
Xia Zhou; Ziyu Liang; Shanyu Qin; Xianxian Ruan; Haixing Jiang
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1945-1954
ISSN:
0301-4851
Subject:
Western blotting; blood serum; centrifugation; coculture; collagen; confocal microscopy; exosomes; humans; immunohistochemistry; liver; liver cirrhosis; molecular biology
Abstract:
... AIM: To investigate the association of serum exosomes miR-574-5p with liver fibrosis, and explore the effect and mechanism of serum exosomes on HSC activation. MATERIALS AND METHODS: Using serum samples collected from healthy adults and patients with liver cirrhosis, we extracted human serum exosomes via ultra-high-speed centrifugation, and co-cultured them with hepatic stellate cells (HSCs) line ...
DOI:
10.1007/s11033-021-07008-2