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You searched for: Journal Molecular biology reports Remove constraint Journal: Molecular biology reports Publication Year 2008 Remove constraint Publication Year: 2008 Source 2008 v.35 no.2 Remove constraint Source: 2008 v.35 no.2
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1. A novel serine protease with clip domain from scallop Chlamys farreri

Author:
Zhu, Ling; Song, Linsheng; Mao, Yuze; Zhao, Jiangmin; Li, Chenghua; Xu, Wei
Source:
Molecular biology reports 2008 v.35 no.2 pp. 257-264
ISSN:
0301-4851
Subject:
prophenoloxidase; 3' untranslated regions; cell adhesion; 5' untranslated regions; polypeptides; disulfide bonds; Micrococcus luteus; open reading frames; Azumapecten farreri; Vibrio anguillarum; gene expression; amino acids; rapid amplification of cDNA ends; signal peptide; scallops; complementary DNA; serine proteinases; immune response; expressed sequence tags; rapid methods; anti-infective properties; hemocytes
Abstract:
... The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of ...
DOI:
10.1007/s11033-007-9078-2
PubMed:
17484057

2. BcMF13, a new reproductive organ-specific gene from Brassica rapa. ssp. chinensis, affects pollen development

Author:
Li, Yanyan; Cao, Jiashu; Huang, Li; Yu, Xiaolin; Xiang, Xun
Source:
Molecular biology reports 2008 v.35 no.2 pp. 207-214
ISSN:
0301-4851
Subject:
Chinese cabbage; Southern blotting; buds; antisense RNA; complementary DNA; males; expressed sequence tags; introns; pollen; gametogenesis; amino acids; gene expression; microspores; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; Brassica rapa subsp. oleifera; databases
Abstract:
... A transcript-derived fragment (GenBank accession number DN237920.1) accumulated in the wild-type flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) was isolated and further investigated. The full length DNA and cDNA of the fragment were cloned by rapid amplification of cDNA ends. The gene, BcMF13, encodes a protein of 73 amino acids and is in ...
DOI:
10.1007/s11033-007-9072-8
PubMed:
17387632

3. Complete sequence and gene organization of the mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis)

Author:
Ji, Xuefeng; Wu, Xiaobing; Yan, Peng; Amato, George
Source:
Molecular biology reports 2008 v.35 no.2 pp. 133-138
ISSN:
0301-4851
Subject:
Crocodylus siamensis; crocodiles; genes; guanine; leaves; mitochondrial DNA; mitochondrial genome; nucleotide sequences; nucleotides; polymerase chain reaction; thymine; transfer RNA
Abstract:
... The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile is similar to most vertebrates in gene component, order, orientation, tRNA struc ...
DOI:
10.1007/s11033-007-9062-x
PubMed:
17294250

4. Coprinus comatus and Ganoderma lucidum interfere with androgen receptor function in LNCaP prostate cancer cells

Author:
Zaidman, Ben-Zion; Wasser, Solomon P.; Nevo, Eviatar; Mahajna, Jamal
Source:
Molecular biology reports 2008 v.35 no.2 pp. 107-117
ISSN:
0301-4851
Subject:
Coprinus comatus; Ganoderma lucidum; androgen receptors; breast neoplasms; cell viability; dose response; ethanol; ethyl acetate; ethyl ether; fungi; interphase; neoplasm cells; prostate-specific antigen; prostatic neoplasms; transcription (genetics)
Abstract:
... In this study, we screened a total of 201 diethyl ether, ethanol, and ethyl acetate fungal Basidiomycetes extracts for anti-androgenic activity. Based on our screened results in combination with the selective inhibition of prostate cancer LNCaP cells, we selected Coprinus comatus and Ganoderma lucidum for further evaluation. We demonstrated that ethanol and ethyl acetate extracts from C. comatus a ...
DOI:
10.1007/s11033-007-9059-5
PubMed:
17431821

5. Expression of puroindoline a enhances leaf rust resistance in transgenic tetraploid wheat

Author:
Luo, Liting; Zhang, Jinrui; Yang, Guangxiao; Li, Yang; Li, Kexiu; He, Guangyuan
Source:
Molecular biology reports 2008 v.35 no.2 pp. 195-200
ISSN:
0301-4851
Subject:
Puccinia recondita; anti-infective properties; bacteria; chromosomes; disease resistance; fungi; genes; greenhouses; hardness; harvesting; leaf rust; microbial growth; proteins; tetraploidy; transgenic plants; wheat; China
Abstract:
... The purouindoline gene (pin) coding for puroindoline proteins (PINs) is located on chromosome 5D, controls grain hardness, and the PINs have in vitro antimicrobial activity against gram-positive (G⁺) bacteria, gram-negative (G⁻) bacteria and fungi. Wheat leaf rust caused by Puccinia triticina is one of the most important fungal diseases for common wheat with AABBDD genomes. Tetraploid wheat (AABB ...
DOI:
10.1007/s11033-007-9070-x
PubMed:
17380426

6. Isolation, sequence identification and tissue expression distribution of three novel porcine genes--RAB14, S35A3 and ITM2A

Author:
Liu, G. Y.; Ge, C. R.; Zhang, X.; Gao, S. Z.
Source:
Molecular biology reports 2008 v.35 no.2 pp. 201-206
ISSN:
0301-4851
Subject:
Dictyostelium discoideum; amino acids; cattle; dogs; gene overexpression; genes; humans; kidneys; large intestine; liver; membrane proteins; mice; muscles; nucleotide sequences; rats; reverse transcriptase polymerase chain reaction; sequence analysis; small intestine; spleen; swine
Abstract:
... The complete coding sequences of three porcine genes--RAB14, S35A3 and ITM2A were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the mouse or other mammals. The nucleotide sequence analysis of these three genes revealed that porcine RAB14 gene encodes a protein of 215 amino acids that contains the conserved putative Ras-r ...
DOI:
10.1007/s11033-007-9071-9
PubMed:
17380425

7. Mango profilin: cloning, expression and cross-reactivity with birch pollen profilin Bet v 2

Author:
Song, Juanjuan; Zhang, Hongyun; Liu, Zhigang; Ran, Pixin
Source:
Molecular biology reports 2008 v.35 no.2 pp. 231-237
ISSN:
0301-4851
Subject:
Betula; Escherichia coli; RNA; affinity chromatography; agarose; allergenicity; allergens; amino acid sequences; amino acids; anaphylaxis; complementary DNA; cross reaction; enzyme-linked immunosorbent assay; fruits; immunoglobulin E; mangoes; open reading frames; patients; pollen; recombinant proteins; reverse transcriptase polymerase chain reaction
Abstract:
... Mango can cause severe anaphylactic reactions. Profilin has been assumed partly responsible for the cross-reactivity between mango fruit and other allergens but has not been finally clarified. In this study, two isoforms of mango fruits profilin were amplified by RT-PCR and 3'RACE from total RNA. Each mango profilin cDNA includes an open reading frame coding for 131 amino acids. The deduced amino ...
DOI:
10.1007/s11033-007-9075-5
PubMed:
17417721

8. Molecular characterization and expression analysis of OsBISERK1, a gene encoding a leucine-rich repeat receptor-like kinase, during disease resistance responses in rice

Author:
Song, Donghui; Li, Guojun; Song, Fengming; Zheng, Zhong
Source:
Molecular biology reports 2008 v.35 no.2 pp. 275-283
ISSN:
0301-4851
Subject:
Magnaporthe grisea; disease resistance; gene expression regulation; genes; genotype; hydrophobicity; leaves; leucine zipper; protein-serine-threonine kinases; rice; seedlings; signal peptide; somatic embryogenesis
Abstract:
... A rice gene, OsBISERK1, encoding a protein belonging to SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) type of leucine-rich repeat receptor-like kinases (LRR-RLKs) was identified. The OsBISERK1 encodes a 624 aa protein with high level of identity to known plant SERKs. OsBISERK1 contains a hydrophobic signal peptide, a leucine zipper, and five leucine-rich repeat motifs in the extracellular domain; t ...
DOI:
10.1007/s11033-007-9080-8
PubMed:
17520342

9. Molecular cloning and expression characteristics of alternative oxidase gene of cotton (Gossypium hirsutum)

Author:
Li, Fang; Zhang, Yi; Wang, Meimei; Zhang, Ying; Wu, Xiaoliang; Guo, Xingqi
Source:
Molecular biology reports 2008 v.35 no.2 pp. 97-105
ISSN:
0301-4851
Subject:
Arabidopsis thaliana; Glycine max; Gossypium hirsutum; Nicotiana tabacum; Northern blotting; Solanum tuberosum; amino acid sequences; amino acids; antimycin A; citrates; cold; complementary DNA; corolla; cotton; cotyledons; exons; hydrophobicity; introns; molecular cloning; molecular weight; multigene family; open reading frames; potassium cyanide; proteins; tissues
Abstract:
... A novel alternative oxidase (AOX) gene derived from cotton (Gossypium hirsutum), designated as GhAOX1, was cloned with RACE-PCR. The full-length cDNA of GhAOX1was 1,298 bp in size, containing a 996 bp open reading frame (ORF) which corresponds to a precursor protein of 332 amino acid residues with a calculated molecular mass of 37.5 kDa. The predicted amino acid sequence exhibited 68.4%, 68.1%, 59 ...
DOI:
10.1007/s11033-007-9058-6
PubMed:
17351819

10. Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians

Author:
Zhu, Ling; Song, Linsheng; Zhang, Huan; Zhao, Jianmin; Li, Chenghua; Xu, Wei
Source:
Molecular biology reports 2008 v.35 no.2 pp. 125-132
ISSN:
0301-4851
Subject:
3' untranslated regions; 5' untranslated regions; Araneus; Argopecten irradians; Drosophila melanogaster; Homo sapiens; Mesocricetus auratus; Mus musculus; Rattus norvegicus; Saccharomyces cerevisiae; Sus scrofa; apoptosis; cDNA libraries; complementary DNA; gene expression; genes; gills; growth and development; hemolymph; molecular cloning; muscles; open reading frames; phylogeny; rapid amplification of cDNA ends; regulatory proteins; scallops; signal peptide; tissues
Abstract:
... Apoptosis is an active process of cell death, which is an integral part of growth and development in multicellular organisms. The defender against cell death 1 (DAD1), the regulatory protein to inhibit the apoptosis process, was first cloned from the bay scallop Argopecten irradians by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA end (RACE). The full-length cDNA ...
DOI:
10.1007/s11033-007-9061-y
PubMed:
17294251

11. Natural Killer Cell Immunoglobulin-like Receptors (KIR) Genotypes in two Arab Populations: Will KIR become a genetic landmark between nations

Author:
Rayes, Roni; Bazarbachi, Ali; Khazen, Georges; Sabbagh, Amira; Zaatari, Ghazi; Mahfouz, Rami
Source:
Molecular biology reports 2008 v.35 no.2 pp. 225-229
ISSN:
0301-4851
Subject:
DNA; genes; genotype; genotyping; loci; nationalities and ethnic groups; natural killer cells; receptors; Lebanon; Middle East
Abstract:
... Genotypic profiles of the Natural killer cell Immunoglobulin-like Receptors (KIR) have been reported to vary among different ethnic groups and this study represents a comparative report on its distribution between two Arabic populations in the Middle East: Lebanese and Palestinians. Our compared population samples included 120 unrelated healthy Lebanese (as per Mahfouz et al.) and a Palestinian po ...
DOI:
10.1007/s11033-007-9074-6
PubMed:
17385087

12. RAPD typing of Aspergillus chevalieri, Aspergillus nidulans, Aspergillus tetrazonus (quadrilineatus) and their teleomorphs using 5'-d[AACGCGCAAC]-3' and 5'-d[CCCGTCAGCA]-3' primers

Author:
Abu Seadah, Akram A.; El Shikh, Mohey Eldin
Source:
Molecular biology reports 2008 v.35 no.2 pp. 89-95
ISSN:
0301-4851
Subject:
Aspergillus chevalieri; Aspergillus nidulans; drug therapy; fungi; neoplasms; organ transplantation; psychological stress; random amplified polymorphic DNA technique; teleomorphs
Abstract:
... Sexuality in fungi has long been a matter of concerns and debates that always necessitated extensive analysis of the relationship between organisms assumed to represent different developmental forms of the same organism. Moreover, mating-virulence correlation and the growing worry of opportunistic fungal infections in immunocompromised states associated with AIDS, cancer chemotherapy and organ tra ...
DOI:
10.1007/s11033-007-9057-7
PubMed:
17268891

13. The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review

Author:
Deng, Weidong; Xi, Dongmei; Mao, Huaming; Wanapat, Metha
Source:
Molecular biology reports 2008 v.35 no.2 pp. 265-274
ISSN:
0301-4851
Subject:
Protozoa; bacteria; denaturing gradient gel electrophoresis; ecosystems; fungi; internal transcribed spacers; microbial ecology; nucleic acid hybridization; quantitative polymerase chain reaction; restriction fragment length polymorphism; ribosomal DNA; ribosomal RNA; rumen; rumen fungi
Abstract:
... This paper analyses the research progress in the use of molecular techniques based on ribosomal RNA and DNA (rRNA/rDNA) for rumen microbial ecosystem since first literature by Stahl et al. (1988). Because rumen microbial populations could be under-estimated by adopting the traditional techniques such as roll-tube technique or most-probable-number estimates, modern molecular techniques based on 16S ...
DOI:
10.1007/s11033-007-9079-1
PubMed:
17484038

14. A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway

Author:
Ai, Jianping; Wang, Yuequn; Tan, Kunrong; Deng, Yun; Luo, Na; Yuan, Wuzhou; Wang, Zequn; Li, Yongqing; Wang, Ying; Mo, Xiaoyang; Zhu, Chuanbing; Yin, Zhaochu; Liu, Mingyao; Wu, Xiushan
Source:
Molecular biology reports 2008 v.35 no.2 pp. 179-187
ISSN:
0301-4851
Subject:
transcription factors; transcriptional activation; complementary DNA; gene overexpression; evolution; humans; signal transduction; mice; genes; cDNA libraries; Danio rerio; heart; Northern blotting; mitogen-activated protein kinase
Abstract:
... It has been reported that mouse Lbh (limb-bud and heart) can regulate cardiac gene expression by modulating the combinatorial activities of key cardiac transcription factors, as well as their individual functions in cardiogenesis. Here we report the cloning and characterization of the human homolog of mouse Lbh gene, hLBH, from a human embryonic heart cDNA library. The cDNA of hLBH is 2927 bp long ...
DOI:
10.1007/s11033-007-9068-4
PubMed:
17390236

15. Group I introns and gnra tetraloops: remnants of 'The RNA world'

Author:
Prathiba, J.; Malathi, R.
Source:
Molecular biology reports 2008 v.35 no.2 pp. 239-249
ISSN:
0301-4851
Subject:
RNA; adenosine; introns; metal ions; prebiotics
Abstract:
... GNRA tetraloops, found in high frequency in natural RNAs, make loop-receptor interactions, stabilizing the tertiary structure of Group I introns, a class of small RNAs. Analyzing 230 Group I introns, to study the distribution and sequence pattern of the GNRA tetraloops, we suggest that these features reflect the ancestral nature of these catalytic molecules, in a prebiotic RNA world. The adenosine ...
DOI:
10.1007/s11033-007-9076-4
PubMed:
17437184

16. Knocking down Wnt9a mRNA levels increases cellular proliferation

Author:
Xiang, Yang; Lin, Ge; Zhang, Qianjun; Tan, Yueqiu; Lu, Guangxiu
Source:
Molecular biology reports 2008 v.35 no.2 pp. 73-79
ISSN:
0301-4851
Subject:
RNA interference; breast neoplasms; cell proliferation; double-stranded RNA; eukaryotic cells; flow cytometry; humans; in situ hybridization; messenger RNA; nucleotide sequences; proteins; small interfering RNA
Abstract:
... Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this study we used pAVU6+27 vectors, which have SalI and XbaI clone sites, to construct ...
DOI:
10.1007/s11033-007-9055-9
PubMed:
17351820

17. Molecular cloning and characterization of a novel SNAP25-type protein gene OsSNAP32 in rice (Oryza sativa L.)

Author:
Bao, Yong-Mei; Wang, Jian-Fei; Huang, Ji; Zhang, Hong-Sheng
Source:
Molecular biology reports 2008 v.35 no.2 pp. 145-152
ISSN:
0301-4851
Subject:
rice; sequence alignment; Oryza sativa; seedlings; Magnaporthe grisea; receptors; roots; barley; hydrogen peroxide; plasma membrane; molecular weight; introns; leaves; blast disease; chromosomes; eukaryotic cells; onions; molecular cloning; abiotic stress; temperature; reverse transcriptase polymerase chain reaction
Abstract:
... The SNAP25-type proteins belong to the superfamily of the SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), and function as important components of the vesical trafficking machinery in eukaryotic cells. In this paper, we report the cloning and expression characterization of OsSNAP32 gene, and the subcellular localization of its encoded protein. The OsSNAP32 gene cont ...
DOI:
10.1007/s11033-007-9064-8
PubMed:
17380428

18. Mutations in the passenger polypeptide can affect its partitioning between mitochondria and cytoplasm

Author:
Pastukh, Viktoriya; Shokolenko, Inna N.; Wilson, Glenn L.; Alexeyev, Mikhail F.
Source:
Molecular biology reports 2008 v.35 no.2 pp. 215-223
ISSN:
0301-4851
Subject:
DNA-directed DNA polymerase; amino acid sequences; amino acid substitution; cytoplasm; polypeptides; superoxide dismutase
Abstract:
... In this study, we report that the partitioning between mitochondria and cytoplasm of two variants, mCherry and DsRed Express (DRE), of the red fluorescent protein, DsRed, fused to one of the six matrix targeting sequences (MTSs) can be affected by both MTS and amino acid substitutions in DsRed. Of the six MTSs tested, MTSs from superoxide dismutase and DNA polymerase gamma failed to direct mCherry ...
DOI:
10.1007/s11033-007-9073-7

19. Primary function analysis of human mental retardation related gene CRBN

Author:
Xin, Wang; Xiaohua, Ni; Peilin, Chen; Xin, Chen; Yaqiong, Sun; Qihan, Wu
Source:
Molecular biology reports 2008 v.35 no.2 pp. 251-256
ISSN:
0301-4851
Subject:
adenosine triphosphate; brain; cell nucleolus; cytoplasm; genes; humans; mutants; mutation; protein structure; proteinases; testes; transfection
Abstract:
... The mutation of human cereblon gene (CRBN) is revealed to be related with mild mental retardation. Since the molecular characteristics of CRBN have not been well presented, we investigated the general properties of CRBN. We analyzed its gene structure and protein homologues. The CRBN protein might belong to a family of adenosine triphosphate (ATP)-dependent Lon protease. We also found that CRBN wa ...
DOI:
10.1007/s11033-007-9077-3
PubMed:
17380424

20. Strand compositional asymmetries in vertebrate large genes

Author:
Wang, Hai-Fang; Hou, Wen-Ru; Niu, Deng-Ke
Source:
Molecular biology reports 2008 v.35 no.2 pp. 163-169
ISSN:
0301-4851
Subject:
essential genes; mutation; somatic cells; transcription (genetics); vertebrates
Abstract:
... Both transcription-associated and replication-associated strand compositional asymmetries have recently been shown in vertebrate genomes. In this paper, we illustrate that transcription-associated strand compositional asymmetries and replication-associated ones coexist in most vertebrate large genes, although in most case the former conceals the latter. Furthermore, we found that the transcription ...
DOI:
10.1007/s11033-007-9066-6
PubMed:
17420956