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- Author:
- Zhu, Ling; Song, Linsheng; Mao, Yuze; Zhao, Jiangmin; Li, Chenghua; Xu, Wei
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 257-264
- ISSN:
- 0301-4851
- Subject:
- prophenoloxidase; 3' untranslated regions; cell adhesion; 5' untranslated regions; polypeptides; disulfide bonds; Micrococcus luteus; open reading frames; Azumapecten farreri; Vibrio anguillarum; gene expression; amino acids; rapid amplification of cDNA ends; signal peptide; scallops; complementary DNA; serine proteinases; immune response; expressed sequence tags; rapid methods; anti-infective properties; hemocytes
- Abstract:
- ... The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of ...
- DOI:
- 10.1007/s11033-007-9078-2
- PubMed:
- 17484057
-
http://dx.doi.org/10.1007/s11033-007-9078-2
- Author:
- Li, Yanyan; Cao, Jiashu; Huang, Li; Yu, Xiaolin; Xiang, Xun
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 207-214
- ISSN:
- 0301-4851
- Subject:
- Chinese cabbage; Southern blotting; buds; antisense RNA; complementary DNA; males; expressed sequence tags; introns; pollen; gametogenesis; amino acids; gene expression; microspores; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; Brassica rapa subsp. oleifera; databases
- Abstract:
- ... A transcript-derived fragment (GenBank accession number DN237920.1) accumulated in the wild-type flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) was isolated and further investigated. The full length DNA and cDNA of the fragment were cloned by rapid amplification of cDNA ends. The gene, BcMF13, encodes a protein of 73 amino acids and is in ...
- DOI:
- 10.1007/s11033-007-9072-8
- PubMed:
- 17387632
-
http://dx.doi.org/10.1007/s11033-007-9072-8
- Author:
- Ji, Xuefeng; Wu, Xiaobing; Yan, Peng; Amato, George
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 133-138
- ISSN:
- 0301-4851
- Subject:
- Crocodylus siamensis; crocodiles; genes; guanine; leaves; mitochondrial DNA; mitochondrial genome; nucleotide sequences; nucleotides; polymerase chain reaction; thymine; transfer RNA
- Abstract:
- ... The complete sequence of mitochondrial genome of Siamensis Crocodile (Crocodylus siamensis) is determined by using PCR amplification, clone and primer-walking sequencing methods. The genome is 16836 bp in size, containing 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mtDNA genome of Siamensis Crocodile is similar to most vertebrates in gene component, order, orientation, tRNA struc ...
- DOI:
- 10.1007/s11033-007-9062-x
- PubMed:
- 17294250
-
http://dx.doi.org/10.1007/s11033-007-9062-x
- Author:
- Zaidman, Ben-Zion; Wasser, Solomon P.; Nevo, Eviatar; Mahajna, Jamal
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 107-117
- ISSN:
- 0301-4851
- Subject:
- Coprinus comatus; Ganoderma lucidum; androgen receptors; breast neoplasms; cell viability; dose response; ethanol; ethyl acetate; ethyl ether; fungi; interphase; neoplasm cells; prostate-specific antigen; prostatic neoplasms; transcription (genetics)
- Abstract:
- ... In this study, we screened a total of 201 diethyl ether, ethanol, and ethyl acetate fungal Basidiomycetes extracts for anti-androgenic activity. Based on our screened results in combination with the selective inhibition of prostate cancer LNCaP cells, we selected Coprinus comatus and Ganoderma lucidum for further evaluation. We demonstrated that ethanol and ethyl acetate extracts from C. comatus a ...
- DOI:
- 10.1007/s11033-007-9059-5
- PubMed:
- 17431821
-
http://dx.doi.org/10.1007/s11033-007-9059-5
- Author:
- Luo, Liting; Zhang, Jinrui; Yang, Guangxiao; Li, Yang; Li, Kexiu; He, Guangyuan
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 195-200
- ISSN:
- 0301-4851
- Subject:
- Puccinia recondita; anti-infective properties; bacteria; chromosomes; disease resistance; fungi; genes; greenhouses; hardness; harvesting; leaf rust; microbial growth; proteins; tetraploidy; transgenic plants; wheat; China
- Abstract:
- ... The purouindoline gene (pin) coding for puroindoline proteins (PINs) is located on chromosome 5D, controls grain hardness, and the PINs have in vitro antimicrobial activity against gram-positive (G⁺) bacteria, gram-negative (G⁻) bacteria and fungi. Wheat leaf rust caused by Puccinia triticina is one of the most important fungal diseases for common wheat with AABBDD genomes. Tetraploid wheat (AABB ...
- DOI:
- 10.1007/s11033-007-9070-x
- PubMed:
- 17380426
-
http://dx.doi.org/10.1007/s11033-007-9070-x
- Author:
- Liu, G. Y.; Ge, C. R.; Zhang, X.; Gao, S. Z.
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 201-206
- ISSN:
- 0301-4851
- Subject:
- Dictyostelium discoideum; amino acids; cattle; dogs; gene overexpression; genes; humans; kidneys; large intestine; liver; membrane proteins; mice; muscles; nucleotide sequences; rats; reverse transcriptase polymerase chain reaction; sequence analysis; small intestine; spleen; swine
- Abstract:
- ... The complete coding sequences of three porcine genes--RAB14, S35A3 and ITM2A were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the mouse or other mammals. The nucleotide sequence analysis of these three genes revealed that porcine RAB14 gene encodes a protein of 215 amino acids that contains the conserved putative Ras-r ...
- DOI:
- 10.1007/s11033-007-9071-9
- PubMed:
- 17380425
-
http://dx.doi.org/10.1007/s11033-007-9071-9
- Author:
- Song, Juanjuan; Zhang, Hongyun; Liu, Zhigang; Ran, Pixin
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 231-237
- ISSN:
- 0301-4851
- Subject:
- Betula; Escherichia coli; RNA; affinity chromatography; agarose; allergenicity; allergens; amino acid sequences; amino acids; anaphylaxis; complementary DNA; cross reaction; enzyme-linked immunosorbent assay; fruits; immunoglobulin E; mangoes; open reading frames; patients; pollen; recombinant proteins; reverse transcriptase polymerase chain reaction
- Abstract:
- ... Mango can cause severe anaphylactic reactions. Profilin has been assumed partly responsible for the cross-reactivity between mango fruit and other allergens but has not been finally clarified. In this study, two isoforms of mango fruits profilin were amplified by RT-PCR and 3'RACE from total RNA. Each mango profilin cDNA includes an open reading frame coding for 131 amino acids. The deduced amino ...
- DOI:
- 10.1007/s11033-007-9075-5
- PubMed:
- 17417721
-
http://dx.doi.org/10.1007/s11033-007-9075-5
- Author:
- Song, Donghui; Li, Guojun; Song, Fengming; Zheng, Zhong
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 275-283
- ISSN:
- 0301-4851
- Subject:
- Magnaporthe grisea; disease resistance; gene expression regulation; genes; genotype; hydrophobicity; leaves; leucine zipper; protein-serine-threonine kinases; rice; seedlings; signal peptide; somatic embryogenesis
- Abstract:
- ... A rice gene, OsBISERK1, encoding a protein belonging to SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) type of leucine-rich repeat receptor-like kinases (LRR-RLKs) was identified. The OsBISERK1 encodes a 624 aa protein with high level of identity to known plant SERKs. OsBISERK1 contains a hydrophobic signal peptide, a leucine zipper, and five leucine-rich repeat motifs in the extracellular domain; t ...
- DOI:
- 10.1007/s11033-007-9080-8
- PubMed:
- 17520342
-
http://dx.doi.org/10.1007/s11033-007-9080-8
- Author:
- Li, Fang; Zhang, Yi; Wang, Meimei; Zhang, Ying; Wu, Xiaoliang; Guo, Xingqi
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 97-105
- ISSN:
- 0301-4851
- Subject:
- Arabidopsis thaliana; Glycine max; Gossypium hirsutum; Nicotiana tabacum; Northern blotting; Solanum tuberosum; amino acid sequences; amino acids; antimycin A; citrates; cold; complementary DNA; corolla; cotton; cotyledons; exons; hydrophobicity; introns; molecular cloning; molecular weight; multigene family; open reading frames; potassium cyanide; proteins; tissues
- Abstract:
- ... A novel alternative oxidase (AOX) gene derived from cotton (Gossypium hirsutum), designated as GhAOX1, was cloned with RACE-PCR. The full-length cDNA of GhAOX1was 1,298 bp in size, containing a 996 bp open reading frame (ORF) which corresponds to a precursor protein of 332 amino acid residues with a calculated molecular mass of 37.5 kDa. The predicted amino acid sequence exhibited 68.4%, 68.1%, 59 ...
- DOI:
- 10.1007/s11033-007-9058-6
- PubMed:
- 17351819
-
http://dx.doi.org/10.1007/s11033-007-9058-6
- Author:
- Zhu, Ling; Song, Linsheng; Zhang, Huan; Zhao, Jianmin; Li, Chenghua; Xu, Wei
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 125-132
- ISSN:
- 0301-4851
- Subject:
- 3' untranslated regions; 5' untranslated regions; Araneus; Argopecten irradians; Drosophila melanogaster; Homo sapiens; Mesocricetus auratus; Mus musculus; Rattus norvegicus; Saccharomyces cerevisiae; Sus scrofa; apoptosis; cDNA libraries; complementary DNA; gene expression; genes; gills; growth and development; hemolymph; molecular cloning; muscles; open reading frames; phylogeny; rapid amplification of cDNA ends; regulatory proteins; scallops; signal peptide; tissues
- Abstract:
- ... Apoptosis is an active process of cell death, which is an integral part of growth and development in multicellular organisms. The defender against cell death 1 (DAD1), the regulatory protein to inhibit the apoptosis process, was first cloned from the bay scallop Argopecten irradians by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA end (RACE). The full-length cDNA ...
- DOI:
- 10.1007/s11033-007-9061-y
- PubMed:
- 17294251
-
http://dx.doi.org/10.1007/s11033-007-9061-y
- Author:
- Rayes, Roni; Bazarbachi, Ali; Khazen, Georges; Sabbagh, Amira; Zaatari, Ghazi; Mahfouz, Rami
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 225-229
- ISSN:
- 0301-4851
- Subject:
- DNA; genes; genotype; genotyping; loci; nationalities and ethnic groups; natural killer cells; receptors; Lebanon; Middle East
- Abstract:
- ... Genotypic profiles of the Natural killer cell Immunoglobulin-like Receptors (KIR) have been reported to vary among different ethnic groups and this study represents a comparative report on its distribution between two Arabic populations in the Middle East: Lebanese and Palestinians. Our compared population samples included 120 unrelated healthy Lebanese (as per Mahfouz et al.) and a Palestinian po ...
- DOI:
- 10.1007/s11033-007-9074-6
- PubMed:
- 17385087
-
http://dx.doi.org/10.1007/s11033-007-9074-6
- Author:
- Abu Seadah, Akram A.; El Shikh, Mohey Eldin
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 89-95
- ISSN:
- 0301-4851
- Subject:
- Aspergillus chevalieri; Aspergillus nidulans; drug therapy; fungi; neoplasms; organ transplantation; psychological stress; random amplified polymorphic DNA technique; teleomorphs
- Abstract:
- ... Sexuality in fungi has long been a matter of concerns and debates that always necessitated extensive analysis of the relationship between organisms assumed to represent different developmental forms of the same organism. Moreover, mating-virulence correlation and the growing worry of opportunistic fungal infections in immunocompromised states associated with AIDS, cancer chemotherapy and organ tra ...
- DOI:
- 10.1007/s11033-007-9057-7
- PubMed:
- 17268891
-
http://dx.doi.org/10.1007/s11033-007-9057-7
- Author:
- Deng, Weidong; Xi, Dongmei; Mao, Huaming; Wanapat, Metha
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 265-274
- ISSN:
- 0301-4851
- Subject:
- Protozoa; bacteria; denaturing gradient gel electrophoresis; ecosystems; fungi; internal transcribed spacers; microbial ecology; nucleic acid hybridization; quantitative polymerase chain reaction; restriction fragment length polymorphism; ribosomal DNA; ribosomal RNA; rumen; rumen fungi
- Abstract:
- ... This paper analyses the research progress in the use of molecular techniques based on ribosomal RNA and DNA (rRNA/rDNA) for rumen microbial ecosystem since first literature by Stahl et al. (1988). Because rumen microbial populations could be under-estimated by adopting the traditional techniques such as roll-tube technique or most-probable-number estimates, modern molecular techniques based on 16S ...
- DOI:
- 10.1007/s11033-007-9079-1
- PubMed:
- 17484038
-
http://dx.doi.org/10.1007/s11033-007-9079-1
- Author:
- Ai, Jianping; Wang, Yuequn; Tan, Kunrong; Deng, Yun; Luo, Na; Yuan, Wuzhou; Wang, Zequn; Li, Yongqing; Wang, Ying; Mo, Xiaoyang; Zhu, Chuanbing; Yin, Zhaochu; Liu, Mingyao; Wu, Xiushan
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 179-187
- ISSN:
- 0301-4851
- Subject:
- transcription factors; transcriptional activation; complementary DNA; gene overexpression; evolution; humans; signal transduction; mice; genes; cDNA libraries; Danio rerio; heart; Northern blotting; mitogen-activated protein kinase
- Abstract:
- ... It has been reported that mouse Lbh (limb-bud and heart) can regulate cardiac gene expression by modulating the combinatorial activities of key cardiac transcription factors, as well as their individual functions in cardiogenesis. Here we report the cloning and characterization of the human homolog of mouse Lbh gene, hLBH, from a human embryonic heart cDNA library. The cDNA of hLBH is 2927 bp long ...
- DOI:
- 10.1007/s11033-007-9068-4
- PubMed:
- 17390236
-
http://dx.doi.org/10.1007/s11033-007-9068-4
- Author:
- Prathiba, J.; Malathi, R.
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 239-249
- ISSN:
- 0301-4851
- Subject:
- RNA; adenosine; introns; metal ions; prebiotics
- Abstract:
- ... GNRA tetraloops, found in high frequency in natural RNAs, make loop-receptor interactions, stabilizing the tertiary structure of Group I introns, a class of small RNAs. Analyzing 230 Group I introns, to study the distribution and sequence pattern of the GNRA tetraloops, we suggest that these features reflect the ancestral nature of these catalytic molecules, in a prebiotic RNA world. The adenosine ...
- DOI:
- 10.1007/s11033-007-9076-4
- PubMed:
- 17437184
-
http://dx.doi.org/10.1007/s11033-007-9076-4
- Author:
- Xiang, Yang; Lin, Ge; Zhang, Qianjun; Tan, Yueqiu; Lu, Guangxiu
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 73-79
- ISSN:
- 0301-4851
- Subject:
- RNA interference; breast neoplasms; cell proliferation; double-stranded RNA; eukaryotic cells; flow cytometry; humans; in situ hybridization; messenger RNA; nucleotide sequences; proteins; small interfering RNA
- Abstract:
- ... Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this study we used pAVU6+27 vectors, which have SalI and XbaI clone sites, to construct ...
- DOI:
- 10.1007/s11033-007-9055-9
- PubMed:
- 17351820
-
http://dx.doi.org/10.1007/s11033-007-9055-9
- Author:
- Bao, Yong-Mei; Wang, Jian-Fei; Huang, Ji; Zhang, Hong-Sheng
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 145-152
- ISSN:
- 0301-4851
- Subject:
- rice; sequence alignment; Oryza sativa; seedlings; Magnaporthe grisea; receptors; roots; barley; hydrogen peroxide; plasma membrane; molecular weight; introns; leaves; blast disease; chromosomes; eukaryotic cells; onions; molecular cloning; abiotic stress; temperature; reverse transcriptase polymerase chain reaction
- Abstract:
- ... The SNAP25-type proteins belong to the superfamily of the SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), and function as important components of the vesical trafficking machinery in eukaryotic cells. In this paper, we report the cloning and expression characterization of OsSNAP32 gene, and the subcellular localization of its encoded protein. The OsSNAP32 gene cont ...
- DOI:
- 10.1007/s11033-007-9064-8
- PubMed:
- 17380428
-
http://dx.doi.org/10.1007/s11033-007-9064-8
- Author:
- Pastukh, Viktoriya; Shokolenko, Inna N.; Wilson, Glenn L.; Alexeyev, Mikhail F.
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 215-223
- ISSN:
- 0301-4851
- Subject:
- DNA-directed DNA polymerase; amino acid sequences; amino acid substitution; cytoplasm; polypeptides; superoxide dismutase
- Abstract:
- ... In this study, we report that the partitioning between mitochondria and cytoplasm of two variants, mCherry and DsRed Express (DRE), of the red fluorescent protein, DsRed, fused to one of the six matrix targeting sequences (MTSs) can be affected by both MTS and amino acid substitutions in DsRed. Of the six MTSs tested, MTSs from superoxide dismutase and DNA polymerase gamma failed to direct mCherry ...
- DOI:
- 10.1007/s11033-007-9073-7
-
http://dx.doi.org/10.1007/s11033-007-9073-7
- Author:
- Xin, Wang; Xiaohua, Ni; Peilin, Chen; Xin, Chen; Yaqiong, Sun; Qihan, Wu
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 251-256
- ISSN:
- 0301-4851
- Subject:
- adenosine triphosphate; brain; cell nucleolus; cytoplasm; genes; humans; mutants; mutation; protein structure; proteinases; testes; transfection
- Abstract:
- ... The mutation of human cereblon gene (CRBN) is revealed to be related with mild mental retardation. Since the molecular characteristics of CRBN have not been well presented, we investigated the general properties of CRBN. We analyzed its gene structure and protein homologues. The CRBN protein might belong to a family of adenosine triphosphate (ATP)-dependent Lon protease. We also found that CRBN wa ...
- DOI:
- 10.1007/s11033-007-9077-3
- PubMed:
- 17380424
-
http://dx.doi.org/10.1007/s11033-007-9077-3
- Author:
- Wang, Hai-Fang; Hou, Wen-Ru; Niu, Deng-Ke
- Source:
- Molecular biology reports 2008 v.35 no.2 pp. 163-169
- ISSN:
- 0301-4851
- Subject:
- essential genes; mutation; somatic cells; transcription (genetics); vertebrates
- Abstract:
- ... Both transcription-associated and replication-associated strand compositional asymmetries have recently been shown in vertebrate genomes. In this paper, we illustrate that transcription-associated strand compositional asymmetries and replication-associated ones coexist in most vertebrate large genes, although in most case the former conceals the latter. Furthermore, we found that the transcription ...
- DOI:
- 10.1007/s11033-007-9066-6
- PubMed:
- 17420956
-
http://dx.doi.org/10.1007/s11033-007-9066-6