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You searched for: Author "Song, Linsheng" Remove constraint Author: "Song, Linsheng" Journal Molecular biology reports Remove constraint Journal: Molecular biology reports Subject complementary DNA Remove constraint Subject: complementary DNA
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1. Identification of a cathepsin D potentially involved in H2A cleavage from scallop Chlamys farreri

Author:
Song, Linsheng,   et al.   ; Li, Chenghua; Zhang, Huan; Li, Ling;     Show all 4 Authors
Source:
Molecular biology reports 2010 v.37 no.3 pp. 1451-1460
ISSN:
0301-4851
Subject:
Chlamys; Vibrio anguillarum; amino acids; aquatic invertebrates; cathepsin D; complementary DNA; expressed sequence tags; fish; hemocytes; messenger RNA; nucleotides; quantitative polymerase chain reaction; rapid amplification of cDNA ends; scallops; signal peptide
Abstract:
... We report here a cDNA and its deduced amino acid sequence encoding a cathepsin D-like, aspartic protease from Chlamys farreri (denoted as CfCD) by expressed sequence tag and rapid amplification of cDNA ends techniques. The cDNA of CfCD consisted of 1,810 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, encoding a short signal peptide of 18 amino acids, a pro-en ...
DOI:
10.1007/s11033-009-9534-2
PubMed:
19381870

2. Identification of a C-type lectin from the bay scallop Argopecten irradians

Author:
Song, Linsheng,   et al.   ; Zhu, Ling; Xu, Wei; Qian, Pei-Yuan;     Show all 4 Authors
Source:
Molecular biology reports 2009 v.36 no.5 pp. 1167-1173
ISSN:
0301-4851
Subject:
Argopecten irradians; Micrococcus; Vibrio; antibacterial properties; complementary DNA; cysteine; expressed sequence tags; hemocytes; hepatopancreas; immune response; lectins; messenger RNA; phagocytosis; polypeptides; scallops; signal peptide
Abstract:
... C-type lectins are Ca²⁺ dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of bay scallops Argopecten irradians (Ai Lec) was identified using expressed sequence tag (EST) and RACE ...
DOI:
10.1007/s11033-008-9293-5

3. Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri

Author:
Song, Linsheng,   et al.   ; Cong, Ming; Ni, Duojiao; Wang, Lingling; Zhao, Jianmin; Qiu, Limei; Li, Ling;     Show all 7 Authors
Source:
Molecular biology reports 2009 v.36 no.6 pp. 1451-1459
ISSN:
0301-4851
Subject:
Chlamys; Vibrio anguillarum; amino acids; complementary DNA; gene expression; genes; heart; kidneys; messenger RNA; molecular cloning; open reading frames; peroxiredoxin; quantitative polymerase chain reaction; scallops; sequence analysis; signal transduction
Abstract:
... Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence co ...
DOI:
10.1007/s11033-008-9335-z
PubMed:
18704754

4. The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress

Author:
Song, Linsheng,   et al.   ; Zhang, Lei; Wang, Lingling; Zhao, Jianmin; Qiu, Limei; Dong, Chaohua; Li, Fengmei; Zhang, Huan; Yang, Guanpin;     Show all 9 Authors
Source:
Molecular biology reports 2010 v.37 no.4 pp. 1763-1771
ISSN:
0301-4851
Subject:
Argopecten irradians; amino acid sequences; amino acids; aquatic environment; cadmium; complementary DNA; copper; expressed sequence tags; gene expression; gene expression regulation; heart; heat shock proteins; heavy metals; hemocytes; kidneys; lead; messenger RNA; open reading frames; pollution; polypeptides; reverse transcriptase polymerase chain reaction; scallops
Abstract:
... Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence ...
DOI:
10.1007/s11033-009-9603-6
PubMed:
19585273

5. A novel serine protease with clip domain from scallop Chlamys farreri

Author:
Song, Linsheng,   et al.   ; Zhu, Ling; Mao, Yuze; Zhao, Jiangmin; Li, Chenghua; Xu, Wei;     Show all 6 Authors
Source:
Molecular biology reports 2008 v.35 no.2 pp. 257-264
ISSN:
0301-4851
Subject:
prophenoloxidase; 3' untranslated regions; cell adhesion; 5' untranslated regions; polypeptides; disulfide bonds; Micrococcus luteus; open reading frames; Azumapecten farreri; Vibrio anguillarum; gene expression; amino acids; rapid amplification of cDNA ends; signal peptide; scallops; complementary DNA; serine proteinases; immune response; expressed sequence tags; rapid methods; anti-infective properties; hemocytes
Abstract:
... The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of ...
DOI:
10.1007/s11033-007-9078-2
PubMed:
17484057

6. Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians

Author:
Song, Linsheng,   et al.   ; Zhu, Ling; Zhang, Huan; Zhao, Jianmin; Li, Chenghua; Xu, Wei;     Show all 6 Authors
Source:
Molecular biology reports 2008 v.35 no.2 pp. 125-132
ISSN:
0301-4851
Subject:
3' untranslated regions; 5' untranslated regions; Araneus; Argopecten irradians; Drosophila melanogaster; Homo sapiens; Mesocricetus auratus; Mus musculus; Rattus norvegicus; Saccharomyces cerevisiae; Sus scrofa; apoptosis; cDNA libraries; complementary DNA; gene expression; genes; gills; growth and development; hemolymph; molecular cloning; muscles; open reading frames; phylogeny; rapid amplification of cDNA ends; regulatory proteins; scallops; signal peptide; tissues
Abstract:
... Apoptosis is an active process of cell death, which is an integral part of growth and development in multicellular organisms. The defender against cell death 1 (DAD1), the regulatory protein to inhibit the apoptosis process, was first cloned from the bay scallop Argopecten irradians by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA end (RACE). The full-length cDNA ...
DOI:
10.1007/s11033-007-9061-y
PubMed:
17294251

7. A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri

Author:
Song, Linsheng,   et al.   ; Song, Xiaoyan; Wang, Lingling; Zhao, Jianmin; Zhang, Huan; Zheng, Peilin; Qiu, Limei; Liu, Xiaolin; Wu, Longtao;     Show all 9 Authors
Source:
Molecular biology reports 2009 v.36 no.6 pp. 1637-1645
ISSN:
0301-4851
Subject:
Chlamys; Vibrio anguillarum; amino acids; complementary DNA; cyclophilins; cyclosporine; expressed sequence tags; gene expression; messenger RNA; nucleotides; open reading frames; peptidylprolyl isomerase; polypeptides; proteins; rapid amplification of cDNA ends; scallops; signal peptide
Abstract:
... Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplif ...
DOI:
10.1007/s11033-008-9363-8
PubMed:
18819019