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- Mudalkar, Shalini, et al. Show all 5 Authors
- Molecular biology reports 2014 v.41 no.1 pp. 113-124
- Escherichia coli; Jatropha curcas; X-radiation; cadmium; copper; genes; heavy metals; leaves; messenger RNA; metal tolerance; metallothionein; molecular cloning; mutants; oilseeds; phytoremediation; quantitative polymerase chain reaction; scanning electron microscopy; seed yield; spectroscopy; yeasts; zinc
- ... In the present study, we have cloned a gene encoding JcMT2a protein from Jatropha curcas L., a promising biofuel tree species. Full length sequence of JcMT2a gene was isolated using RACE PCR. Heterologous expression of JcMT2a in Escherichia coli and its purification has shown distinct bands corresponding to the GST and GST-fused JcMT2a protein. Significant tolerance was observed in E. coli cells e ...