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- Ferrantelli, Flavia; Manfredi, Francesco; Chiozzini, Chiara; Anticoli, Simona; Olivetta, Eleonora; Arenaccio, Claudia; Federico, Maurizio
- Molecular biotechnology 2018 v.60 no.11 pp. 773-782
- DNA; Human immunodeficiency virus 1; RNA; animals; antigen-presenting cells; antigens; etiological agents; eukaryotic cells; exosomes; hepatitis B; immune response; immunization; mutants; neoplasms; translation (genetics); vaccines
- ... Eukaryotic cells constitutively produce nanovesicles of 50–150 nm of diameter, referred to as exosomes, upon release of the contents of multivesicular bodies (MVBs). We recently characterized a novel, exosome-based way to induce cytotoxic T lymphocyte (CTL) immunization against full-length antigens. It is based on DNA vectors expressing products of fusion between the exosome-anchoring protein Nef ...
- Demir, Yeliz; Dikbaş, Neslihan; Beydemir, Şükrü
- Molecular biotechnology 2018 v.60 no.11 pp. 783-790
- Lactobacillus coryniformis; amino acids; animals; calcium; catalytic activity; cheeses; cobalt; copper; energy; hydrolysis; lead; magnesium; metal ions; minerals; molecular weight; myo-inositol; nickel; pH; phosphates; phosphorus; phytases; phytic acid; polyacrylamide gel electrophoresis; silver; sodium dodecyl sulfate; substrate specificity; temperature; zinc
- ... Phytase (myo-inositol hexaphosphate phosphohydrolase) belongs to phosphatases. It catalyzes the hydrolysis of phytate to less-phosphorylated inorganic phosphates and phytate. Phytase is used primarily for the feeding of simple hermit animals in order to increase the usability of amino acids, minerals, phosphorus and energy. In the present study, phytase isolation from the Lactobacillus coryniformi ...