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- Ferrantelli, Flavia; Manfredi, Francesco; Chiozzini, Chiara; Anticoli, Simona; Olivetta, Eleonora; Arenaccio, Claudia; Federico, Maurizio
- Molecular biotechnology 2018 v.60 no.11 pp. 773-782
- DNA; Human immunodeficiency virus 1; RNA; animals; antigen-presenting cells; antigens; etiological agents; eukaryotic cells; exosomes; hepatitis B; immune response; immunization; mutants; neoplasms; translation (genetics); vaccines
- ... Eukaryotic cells constitutively produce nanovesicles of 50–150 nm of diameter, referred to as exosomes, upon release of the contents of multivesicular bodies (MVBs). We recently characterized a novel, exosome-based way to induce cytotoxic T lymphocyte (CTL) immunization against full-length antigens. It is based on DNA vectors expressing products of fusion between the exosome-anchoring protein Nef ...
- Potocki, Wojciech; Negri, Alessandro; Peszyńska-Sularz, Grażyna; Hinc, Krzysztof; Obuchowski, Michał; Iwanicki, Adam
- Molecular biotechnology 2018 v.60 no.11 pp. 810-819
- Bacillus subtilis; Clostridium difficile; adjuvants; antibodies; antigens; endospores; feces; human resources; humans; immune response; immunoglobulin A; interleukin-1beta; mice; recombinant fusion proteins; vaccination; vaccines
- ... Mucosal immunizations are convenient ways of vaccination, which do not require any trained personnel for administration. One of the major challenges for developing an effective mucosal vaccine is finding appropriate adjuvant. Bacillus subtilis endospores have been shown to help solving these obstacles while serving as a platform for presentation of both, antigens and adjuvants. In this study, we h ...