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You searched for: Journal Molecular biotechnology Remove constraint Journal: Molecular biotechnology Publication Year 2014 Remove constraint Publication Year: 2014 Source 2014 v.56 no.11 Remove constraint Source: 2014 v.56 no.11 Subject Brazil Remove constraint Subject: Brazil
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1. A Specific Endogenous Reference for Genetically Modified Common Bean (Phaseolus vulgaris L.) DNA Quantification by Real-Time PCR Targeting Lectin Gene

Author:
Venturelli, Gustavo L.; Brod, Fábio C. A.; Rossi, Gabriela B.; Zimmermann, Naíra F.; Oliveira, Jaison P.; Faria, Josias C.; Arisi, Ana C. M.
Source:
Molecular biotechnology 2014 v.56 no.11 pp. 1060-1068
ISSN:
1073-6085
Subject:
DNA; Phaseolus vulgaris; arcelin; beans; commercialization; detection limit; gene pool; genes; genetically modified organisms; green chemistry; haploidy; hydrolysis; phaseolin; quantitative polymerase chain reaction; Andes region; Brazil
Abstract:
... The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new taxon-specific endogenous reference quantification assay was developed for Phase ...
DOI:
10.1007/s12033-014-9786-5
PubMed:
25078400