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- Barucca, A.; Capitani, M.; Cesca, M.; Tomassoni, D.; Kazmi, U.; Concetti, F.; Vincenzetti, L.; Concetti, A.; Venanzi, F. M.
- Molecular biotechnology 2014 v.56 no.11 pp. 1032-1039
- antigens; clinical trials; cost effectiveness; immunization; melanoma; mice; molecular weight; monoclonal antibodies; plasmids; polypeptides; recombinant DNA; recombinant vaccines
- ... Anti-idiotypic MK2-23 monoclonal antibody (anti-Id MK2-23 mAb), which mimics the high molecular weight melanoma-associated antigen (HMW-MAA), has been used to implement active immunotherapy against melanoma. However, due to safety and standardization issues, this approach never entered extensive clinical trials. In the present study, we investigated the usage of DNA vaccines as an alternative to M ...
- Rai, Jay; Pemmasani, J. Kalyani; Voronovsky, Andriy; Jensen, Ida S.; Manavalan, Arulmani; Nyengaard, Jens R.; Golas, Monika M.; Sander, Bjoern
- Molecular biotechnology 2014 v.56 no.11 pp. 992-1003
- Saccharomyces cerevisiae; genes; homologous recombination; mitochondria; nucleotide sequences; plasmids; polymerase chain reaction; proteins; pyruvate dehydrogenase (lipoamide); yeasts
- ... Peptide sequences fused to a gene of interest facilitate the isolation of proteins or protein complexes from cell extracts. In the case of fluorescent protein tags, the tagged protein can be visually localized in living cells. To tag endogenous genes, PCR-based homologous recombination is a powerful approach used in the yeast Saccharomyces cerevisiae. This approach uses short, homologous DNA seque ...