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- Sharma, Cynthia M., et al. Show all 7 Authors
- Molecular cell 2018 v.69 no.5 pp. 893-905.e7
- Campylobacter jejuni; DNA; binding sites; gene editing; messenger RNA; mutational analysis; nucleases
- ... Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter jejuni Cas9 (CjCas9) can bind and cleave complementary endogenous mRNAs in a crRNA-dependent manner. Approximately 100 tr ...