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- TAYLOR, D. LEE; BOOTH, MICHAEL G.; MCFARLAND, JACK W.; HERRIOTT, IAN C.; LENNON, NIALL J.; NUSBAUM, CHAD; MARR, THOMAS G.
- Molecular ecology resources 2008 v.8 no.4 pp. 742-752
- Zygomycota, etc ; Ascomycota; Basidiomycota; Chytridiomycota; DNA; fungi; high-throughput nucleotide sequencing; internal transcribed spacers; phylogeny; restriction fragment length polymorphism; soil; space and time; statistics; Show all 13 Subjects
- ... High throughput sequencing methods are widely used in analyses of microbial diversity, but are generally applied to small numbers of samples, which precludes characterization of patterns of microbial diversity across space and time. We have designed a primer‐tagging approach that allows pooling and subsequent sorting of numerous samples, which is directed to amplification of a region spanning the ...