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- VAN DER KAMP, MARC W., et al. Show all 8 Authors
- Molecular plant pathology 2011 v.12 no.1 pp. 21-30
- tomatoes; disulfide bonds; proteins; Komagataella pastoris; bioactive properties; cysteine proteinase inhibitors; alanine; hypersensitive response; site-directed mutagenesis; cysteine; Passalora fulva
- ... The Cladosporium fulvum Avr2 effector is a novel type of cysteine protease inhibitor with eight cysteine residues that are all involved in disulphide bonds. We have produced wild-type Avr2 protein in Pichia pastoris and determined its disulphide bond pattern. By site-directed mutagenesis of all eight cysteine residues, we show that three of the four disulphide bonds are required for Avr2 stability ...