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- Wang, De-Goo; Brewster, Jeffery D.; Paul, Moushumi; Tomasula, Peggy M.
- Molecules 2015 v.20 no.4 pp. 6048-6059
- Listeria innocua; Listeria monocytogenes; additives; detection limit; dimethyl sulfoxide; genome; loop-mediated isothermal amplification; nucleotide sequences; polymerase chain reaction
- ... The technique of loop-mediated isothermal amplification (LAMP) utilizes 4 (or 6) primers targeting 6 (or 8) regions within a fairly small segment of a genome for amplification, with concentration higher than that used in traditional PCR methods. The high concentrations of primers used leads to an increased likelihood of non-specific amplification induced by primer dimers. In this study, a set of L ...