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1. A questionnaire survey on helminth control on horse farms in Brandenburg, Germany and the assessment of risks caused by different kinds of management

Author:
Hinney, Barbara; Wirtherle, Nicole Catherine; Kyule, Moses; Miethe, Norbert; Zessin, Karl-Hans; Clausen, Peter-Henning
Source:
Parasitology research 2011 v.109 no.6 pp. 1625-1635
ISSN:
0932-0113
Subject:
foals; lactones; pastures; farms; feces; multivariate analysis; cleaning; helminths; sampling; models; risk factors; control methods; anthelmintics; risk reduction; eggs; hygiene; farm structure; questionnaires; surveys; deworming; Germany
Abstract:
... Control strategies for horse helminths are controversially discussed, and no ideal strategy exists. Presently, the spreading of anthelmintic resistance against all three classes of anthelmintics is of special concern. Advice on how to slow the development of anthelmintic resistance does not seem to have reached the majority of horse owners yet. In our study, we wanted to capture the current standa ...
DOI:
10.1007/s00436-011-2434-0
PubMed:
21559764

2. Characterization of Sarcocystis fusiformis based on sequencing and PCR-RFLP in water buffalo (Bubalus bubalis) in Iran

Author:
Oryan, Ahmad; Sharifiyazdi, Hassan; Khordadmehr, Monire; Larki, Sara
Source:
Parasitology research 2011 v.109 no.6 pp. 1563-1570
ISSN:
0932-0113
Subject:
Sarcocystis; buffaloes; cats; dogs; enzymes; genes; genetic heterogeneity; hosts; loci; nucleotide sequences; ribosomal DNA; sarcocystosis; sequence analysis; Iran
Abstract:
... Four Sarcocystis species, i.e., Sarcocystis fusiformis and Sarcocystis buffalonis with cats as definitive hosts, Sarcocystis levinei with dogs as definitive host, and Sarcocystis dubeyi with unknown definitive host, have previously been described from water buffalo (Bubalus bubalis). The aim of the present study was genetic characterization of the causative agent(s) of water buffalo sarcocystosis ...
DOI:
10.1007/s00436-011-2412-6
PubMed:
21526403

3. Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens

Author:
Sun, Haibo; Sun, Linchun; He, Ji; Shen, Bo; Yu, Jing; Chen, Chen; Yang, Mifang; Sun, Yan; Zhang, Donghui; Ma, Lei; Zhu, Changliang
Source:
Parasitology research 2011 v.109 no.6 pp. 1689-1697
ISSN:
0932-0113
Subject:
Culex pipiens pallens; amino acids; deltamethrin; gene expression regulation; genes; open reading frames; polymerase chain reaction; ribosomal proteins; small interfering RNA; viability
Abstract:
... The 40S ribosomal protein S29 (RPS29) was shown differentially expressed in deltamethrin (DM)-susceptible and resistant Culex pipiens pallens previously. Here, we cloned RPS29 from this mosquito. An open reading frame (ORF) of 171 bp was found to encode a putative protein with 56 amino acid residues, which shares more than 90% identities with other mosquito RPS29 genes. Moreover, quantitative real ...
DOI:
10.1007/s00436-011-2443-z
PubMed:
21562761

4. Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process

Author:
Kar, Sirri; Daugschies, Arwid; Cakmak, Ayse; Yilmazer, Nadim; Dittmar, Katja; Bangoura, Berit
Source:
Parasitology research 2011 v.109 no.6 pp. 1719-1723
ISSN:
0932-0113
Subject:
Cryptosporidium parvum; bile; excystation; oocysts; sodium; viability
Abstract:
... This study was conducted as a comparative evaluation of time-dependent changes in the viability of purified Cryptosporidium parvum oocysts by means of different excystation methods. Oocyst samples were 2 weeks to 12 months old and were treated with bile or sodium taurocholate, partly after pretreatment with hypochlorite. Pretreatment markedly enhanced the excystation of younger oocyst samples but ...
DOI:
10.1007/s00436-011-2442-0
PubMed:
21603913

5. Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy

Author:
Domenis, Lorenzo; Peletto, Simone; Sacchi, Luciano; Clementi, Emanuela; Genchi, Marco; Felisari, Lucia; Felisari, Carla; Mo, Patrizia; Modesto, Paola; Zuccon, Fabio; Campanella, Chiara; Maurella, Cristiana; Guidetti, Cristina; Acutis, Pier Luigi
Source:
Parasitology research 2011 v.109 no.6 pp. 1677-1687
ISSN:
0932-0113
Subject:
Canidae; Primates; Sarcocystis cruzi; cats; cattle; cattle breeds; diaphragm; electron microscopy; esophagus; farming systems; farms; genes; heart; humans; intermediate hosts; monitoring; mountains; nucleotide sequences; parasites; pastures; public health; raw meat; ribosomal RNA; risk factors; sewage systems; Italy
Abstract:
... The aim of this study was to determine the prevalence of sarcosporidiosis in semi-intensively bred cattle in northwestern Italy. A diagnostic protocol was setup in which infected animals were identified by rapid histological examination of the esophagus, diaphragm, and heart and the detected Sarcocystis spp. were subsequently typed using conventional electron microscopy in combination with molecul ...
DOI:
10.1007/s00436-011-2441-1
PubMed:
21556683

6. Do secretions from the uropygial gland of birds attract biting midges and black flies?

Author:
Martínez-de la Puente, Josué; Rivero-de Aguilar, Juan; del Cerro, Sara; Argüello, Anastasio; Merino, Santiago
Source:
Parasitology research 2011 v.109 no.6 pp. 1715-1718
ISSN:
0932-0113
Subject:
Ceratopogonidae; Columba livia; Simuliidae; flight; insect nests; insects; monitoring; nest boxes; pigeons; secretion; traps; uropygial gland
Abstract:
... Bird susceptibility to attacks by blood-sucking flying insects could be influenced by urogypial gland secretions. To determine the effect of these secretions on biting midges and black flies, we set up a series of tests. First, we placed uropygial gland secretions from blue tit Cyanistes caeruleus broods inside empty nest boxes while empty nest boxes without gland secretions were treated as contro ...
DOI:
10.1007/s00436-011-2436-y
PubMed:
21614548

7. Efficacy of sulfonamides and Baycox® against Isospora suis in experimental infections of suckling piglets

Author:
Joachim, Anja; Mundt, Hans-Christian
Source:
Parasitology research 2011 v.109 no.6 pp. 1653-1659
ISSN:
0932-0113
Subject:
oocysts; parasites; coccidiosis; diarrhea; body weight; Isospora suis; sulfamethazine; suckling; prepatent period; oral administration; piglets; excretion; half life
Abstract:
... Sulfonamide treatment of piglets against neonatal coccidiosis has frequently been suggested in the literature. In order to evaluate the efficacy of sulfonamides against experimental Isospora suis infections in suckling piglets (oral infection with 1,500 sporulated oocysts of I. suis per piglet on the fourth day of life), two trials were conducted. In trial I, oral sulfadimidine (group Sulfa-Oral) ...
DOI:
10.1007/s00436-011-2438-9
PubMed:
21556685

8. First confirmed report of outbreak of malignant ovine theileriosis among goats in Sudan

Author:
Taha, Khalid M.; Salih, Diaeldin A.; Ahmed, Bukhari M.; Enan, Khalid A.; Ali, Awadia M.; ElHussein, AbdelRahim M.
Source:
Parasitology research 2011 v.109 no.6 pp. 1525-1527
ISSN:
0932-0113
Subject:
Hyalomma anatolicum; Theileria; farms; flocks; goats; immunologic techniques; polymerase chain reaction; sheep; theileriosis; ticks; towns; Sudan
Abstract:
... An outbreak of malignant ovine theileriosis among goats was confirmed and documented. In this outbreak, 16 out of 22 (72.7%) goats died within 4 days showing clinical signs of malignant ovine theileriosis as well as in the postmortem findings. The goats were reared in a mixed flock with sheep in Atbara Town, Northern Sudan. The infection was detected microscopically and confirmed serologically by ...
DOI:
10.1007/s00436-011-2428-y
PubMed:
21537979

9. Genetic diversity of the malaria vaccine candidate merozoite surface protein 1 gene of Plasmodium vivax field isolates in Republic of Korea

Author:
Han, Eun-Taek; Wang, Yue; Lim, Chae Seung; Cho, Jun Hwi; Chai, Jong-Yil
Source:
Parasitology research 2011 v.109 no.6 pp. 1571-1576
ISSN:
0932-0113
Subject:
Plasmodium vivax; antigens; gene frequency; genes; genetic variation; malaria; parasites; protein isolates; surface proteins; vaccines; South Korea
Abstract:
... The Plasmodium vivax merozoite surface protein 1 (Pvmsp-1) locus codes for a major asexual blood-stage antigen currently proposed as a malaria vaccine candidate antigen. However, extensive polymorphism of this protein has been observed in isolates from different geographical areas. Here, we investigate the extent and the frequency of allelic diversity at the Pvmsp-1 locus in field isolates collect ...
DOI:
10.1007/s00436-011-2413-5
PubMed:
21556687

10. Genetic polymorphism at the C-terminal domain (region III) of knob-associated histidine-rich protein (KAHRP) of Plasmodium falciparum in isolates from Iran

Author:
Mardani, Ahmad; Keshavarz, Hossein; Heidari, Aliehsan; Hajjaran, Homa; Raeisi, Ahmad; Khorramizadeh, Mohammad Reza
Source:
Parasitology research 2011 v.109 no.6 pp. 1647-1652
ISSN:
0932-0113
Subject:
Plasmodium falciparum; alleles; genetic polymorphism; genetic variation; malaria; molecular weight; polymerase chain reaction; proteins; therapeutics; vaccines; virulence; Iran
Abstract:
... The knob-associated histidine-rich protein (KAHRP) plays a major role in the virulence of Plasmodium falciparum and is one of the targets for molecular therapy. The primary structure of KAHRP of P. falciparum consists of three domains (regions I–III), of which the C-terminal domain (region III) is the most polymorphic segment of this protein. One of the main obstacles is genetic diversity in desig ...
DOI:
10.1007/s00436-011-2437-x
PubMed:
21594767

11. Helminths of the kelp gull, Larus dominicanus, from the northern Patagonian coast

Author:
Diaz, Julia Inés; Cremonte, Florencia; Navone, Graciela Teresa
Source:
Parasitology research 2011 v.109 no.6 pp. 1555-1562
ISSN:
0932-0113
Subject:
Acanthocephala; Acuariidae; Anisakidae; Dicrocoeliidae; Dilepididae; Diplostomidae; Echinostomatidae; Larus; Microphallidae; Philophthalmidae; coasts; gastrointestinal system; helminths; hosts; macroalgae; Argentina
Abstract:
... The helminth community of Larus dominicanus (Charadriiformes) is reported from Península Valdés, on the Patagonian coast, Argentina. From March 2000 to April 2004, 29 kelp gulls were collected and dissected. All the birds were parasitized by at least 2 of 18 parasite species: 2 cestodes (1 Tetrabothriidae, 1 Dilepididae), 10 trematodes (2 Echinostomatidae, 3 Microphallidae, 2 Gymnophallidae, 1 Phi ...
DOI:
10.1007/s00436-011-2396-2
PubMed:
21556688

12. Improved detection of canine Angiostrongylus vasorum infection using real-time PCR and indirect ELISA

Author:
Jefferies, Ryan; Morgan, Eric R.; Helm, Jenny; Robinson, Matthew; Shaw, Susan E.
Source:
Parasitology research 2011 v.109 no.6 pp. 1577-1583
ISSN:
0932-0113
Subject:
Angiostrongylus vasorum; Crenosoma; DNA; Dirofilaria immitis; EDTA (chelating agent); Toxocara canis; antigens; blood sampling; blood serum; dogs; enzyme-linked immunosorbent assay; feces; hosts; internal transcribed spacers; lungs; mucus; parasites; polymerase chain reaction; proteins
Abstract:
... This study reports the development of a real-time PCR assay and an indirect ELISA to improve on current detection of canine Angiostrongylus vasorum infection. A highly specific fluorescent probe-based, real-time PCR assay was developed to target the A. vasorum second internal transcribed spacer region and detected DNA in EDTA blood, lung tissue, broncho-alveolar larvage fluid, endotracheal mucus, ...
DOI:
10.1007/s00436-011-2414-4
PubMed:
21537986

13. Insecticidal properties of phenols on Culex quinquefasciatus Say and Musca domestica L

Author:
Pavela, Roman
Source:
Parasitology research 2011 v.109 no.6 pp. 1547-1553
ISSN:
0932-0113
Subject:
Culex quinquefasciatus; Musca domestica; acute effects; acute toxicity; carvacrol; insecticidal properties; lethal dose 50; probit analysis; salicylic acid; thymol; toxicity testing
Abstract:
... Thirteen simple phenols and 8 phenolic acids were tested for toxicity to Culex quinquefasciatus larvae and Musca domestica adults. It was found that while the phenolic acids (except salicylic acid) showed little or no effect on acute toxicity, all the tested simple phenols caused mortality within 24 h after application. Lethal doses for acute toxicity of C. quinquefasciatus were successfully estim ...
DOI:
10.1007/s00436-011-2395-3
PubMed:
21523422

14. In vitro trematocidal effects of crude alcoholic extracts of Artemisia annua, A. absinthium, Asimina triloba, and Fumaria officinalis : Trematocidal plant alcoholic extracts

Author:
Ferreira, Jorge F. S.; Peaden, Paul; Keiser, Jennifer
Source:
Parasitology research 2011 v.109 no.6 pp. 1585-1592
ISSN:
0932-0113
Subject:
Artemisia absinthium; Artemisia annua; Asimina triloba; Echinostoma caproni; Fasciola hepatica; Fumaria officinalis; Schistosoma mansoni; acetogenins; animal parasites and pests; artemisinin; bioactive properties; chromatography; food safety; human health; humans; methanol; mortality; plant extracts; praziquantel; synergism; trematode infections; triclabendazole
Abstract:
... Trematode infections negatively affect human and livestock health, and threaten global food safety. The only approved human anthelmintics for trematodiasis are triclabendazole and praziquantel with no alternative drugs in sight. We tested six crude plant extracts against adult Schistosoma mansoni, Fasciola hepatica, and Echinostoma caproni in vitro. Mortality was best achieved by ethanolic extract ...
DOI:
10.1007/s00436-011-2418-0
PubMed:
21562762

15. Is a vertebrate a better host for a parasite than an invertebrate host? Fecundity of Proctoeces cf lintoni (Digenea: Fellodistomidae), a parasite of fish and gastropods in northern Chile

Author:
Oliva, Marcelo Enrique; Alvarez, Carlos
Source:
Parasitology research 2011 v.109 no.6 pp. 1731-1734
ISSN:
0932-0113
Subject:
Gastropoda; fecundity; fish; hosts; parasites; Chile
Abstract:
... The fecundity, the relationship between fecundity and worm size, as well as abundance of the adult worm Proctoeces cf. lintoni (Digenea: Fellodistomidae) in four gastropod hosts (keyhole limpets of the genus Fissurella) and one vertebrate host, the clingfish Sicyases sanguineus from northern Chile, were compared. Worms obtained from Fissurella latimarginata were smaller than those from Fissurella ...
DOI:
10.1007/s00436-011-2489-y
PubMed:
21681629

16. Larvicidal activities of Knema attenuata (Hook. f. & Thomson) Warb. (Myristicaceae) extracts against Aedes albopictus Skuse and Anopheles stephensi Liston

Author:
Vinayachandra,; Shwetha, R.; Chandrashekar, K. R.
Source:
Parasitology research 2011 v.109 no.6 pp. 1671-1676
ISSN:
0932-0113
Subject:
Aedes albopictus; Anopheles stephensi; Myristicaceae; alkaloids; chloroform; ethanol; flavonoids; glycolipids; hexane; insect larvae; lethal concentration 50; mortality; mosquito-borne diseases; pesticidal plants; resins; saponins; seeds; steroids; tannins; terpenoids; toxicity; vector control; India
Abstract:
... In recent years, uses of environment friendly and biodegradable natural insecticides of plant origin have received renewed attention as agents for vector control. The present study was undertaken to investigate the effect of aril and kernel extracts of Knema attenuata (Hook. f. & Thomson) Warb. (Myristicaceae) on larvae of Aedes albopictus Skuse and Anopheles stephensi Liston under laboratory cond ...
DOI:
10.1007/s00436-011-2440-2
PubMed:
21559763

17. Microsatellite analysis of Fasciola spp. in Egypt

Author:
Dar, Yasser; Amer, Said; Courtioux, Bertrand; Dreyfuss, Gilles
Source:
Parasitology research 2011 v.109 no.6 pp. 1741-1744
ISSN:
0932-0113
Subject:
Fasciola gigantica; Fasciola hepatica; alleles; flukes; genetic markers; genetic polymorphism; genetic variation; geographical distribution; helminthiasis; host-parasite relationships; hosts; loci; microsatellite repeats; pathogen identification; Cameroon; Egypt
Abstract:
... Recently, the topic of diversity in Fasciola population in Egypt is controversial. The present study was performed to study the genetic diversity of isolated flukes based on microsatellites markers. Fasciola worms were collected from different hosts and geographical locations in Egypt. Control samples of Fasciola hepatica from France as well as Fasciola gigantica from Cameroon were included in the ...
DOI:
10.1007/s00436-011-2520-3
PubMed:
21744021

18. Modulation of mouse macrophage proteome induced by Toxoplasma gondii tachyzoites in vivo

Author:
Zhou, D. H.; Yuan, Z. G.; Zhao, F. R.; Li, H. L.; Zhou, Y.; Lin, R. Q.; Zou, F. C.; Song, H. Q.; Xu, M. J.; Zhu, X. Q.
Source:
Parasitology research 2011 v.109 no.6 pp. 1637-1646
ISSN:
0932-0113
Subject:
Toxoplasma gondii; arachidonic acid; cathepsin S; desorption; gene banks; hosts; humans; immune response; ionization; lamps; lasers; macrophages; mass spectrometry; metabolism; mice; parasites; pathogenesis; prediction; proteins; proteome; specific pathogen-free animals; tachyzoites; two-dimensional gel electrophoresis
Abstract:
... Toxoplasma gondii is an obligate intracellular protozoan parasite, which can invade and multiply within the macrophages of humans and most warm-blooded animals. Macrophages are important effector cells for the control and killing of intracellular T. gondii, and they may also serve as long-term host cells for the replication and survival of the parasite. In the present study, we explored the proteo ...
DOI:
10.1007/s00436-011-2435-z
PubMed:
21584632

19. Molecular characterization of SUMO E2 conjugation enzyme: differential expression profile in Schistosoma mansoni

Author:
Pereira, Roberta Verciano; Cabral, Fernanda J.; Gomes, Matheus S.; Baba, Elio H.; Jannotti-Passos, Liana K.; Carvalho, Omar; Rodrigues, Vanderlei; Afonso, Robson José Cássia Franco; Castro-Borges, William; Guerra-Sá, Renata
Source:
Parasitology research 2011 v.109 no.6 pp. 1537-1546
ISSN:
0932-0113
Subject:
Schistosoma mansoni; Western blotting; antibodies; databases; enzymes; gene expression regulation; messenger RNA; post-translational modification; proteins; rats; schistosomula; signal transduction
Abstract:
... SUMO-dependent post-translational modification is implicated in a variety of cellular functions including gene expression regulation, nuclear sub-localization, and signal transduction. Conjugation of SUMO to other proteins occurs in a similar process to ubiquitination, which involves three classes of enzymes: an E1 activating, an E2 conjugating, and an E3 target-specific ligase. Ubc9 is the unique ...
DOI:
10.1007/s00436-011-2394-4
PubMed:
21573813

20. Myxobolus harikensis sp. nov. (Myxozoa: Myxobolidae) infecting fins of Cirrhina mrigala (Ham.)—an Indian major carp in Harike Wetland, Punjab (India)

Author:
Kaur, Harpreet; Singh, Ranjeet
Source:
Parasitology research 2011 v.109 no.6 pp. 1699-1705
ISSN:
0932-0113
Subject:
Cirrhinus cirrhosus; Myxobolus; body cavities; carp; fins; freshwater fish; new species; spores; wetlands; India
Abstract:
... During a study of myxosporean parasites of freshwater fishes of Punjab Wetlands, India, one new myxosporean species, Myxobolus harikensis sp. nov. was recorded from the caudal fin of Cirrhina mrigala (Ham.) (Cypriniformes: Cyprinidae). Plasmodia are small, white, spherical-to-rounded and are present on the caudal fin (in between the fin rays) having two to five spores per plasmodium. Spores of M. ...
DOI:
10.1007/s00436-011-2445-x
PubMed:
21584631