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You searched for: Journal Phytochemistry Remove constraint Journal: Phytochemistry Publication Year 2009 Remove constraint Publication Year: 2009 Source 2009 v.70 no.11-12 Remove constraint Source: 2009 v.70 no.11-12
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1. A comparison of citrus blossom volatiles

Author:
Jabalpurwala, Fatima A.; Smoot, John M.; Rouseff, Russell L.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1428-1434
ISSN:
0031-9422
Subject:
flowering; limes; volatile organic compounds; nitrogen compounds; Citrus maxima; spectral analysis; Citrus limon; pollinating insects; lemons; Citrus reticulata; mandarins; Citrus paradisi; grapefruits; Citrus aurantium; Citrus aurantiifolia; principal component analysis; plant-insect relations; insect pollination; Citrus hystrix; chemical composition; monoterpenoids; oranges; Citrus sinensis; pummelos
Abstract:
... The objective of this study was to identify the major volatiles and their relative concentrations in intact grapefruit, sweet orange, sour orange, mandarin, lemon, lime and pummelo blossoms. Volatiles from freshly picked blossoms were collected and concentrated using static headspace solid-phase microextraction and then separated and identified using GC-MS. Seventy volatiles were detected, 66 iden ...
DOI:
10.1016/j.phytochem.2009.07.031
PubMed:
19747702

2. An experimental approach to study the biosynthesis of brominated metabolites by the red algal genus Laurencia

Author:
Suzuki, Minoru; Takahashi, Yoshinori; Nakano, Satoru; Abe, Tsuyoshi; Masuda, Michio; Ohnishi, Toshiyuki; Noya, Yoichi; Seki, Koh-ichi
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1410-1415
ISSN:
0031-9422
Subject:
algae; halogenated hydrocarbons; Laurencia; bromination
Abstract:
... The production of labeled brominated metabolites with radioactive 82Br in Laurencia species was investigated as part of a study of the biosynthesis of halogenated metabolites from species belonging to the red algal genus Laurencia (Rhodomelaceae, Ceramiales). Radiobromide [82Br], thin-layer chromatography (TLC), and TLC-autoradioluminography (ARLG) were used. When cultured in artificial seawater m ...
DOI:
10.1016/j.phytochem.2009.07.038
PubMed:
19733868

3. Antihyperglycemic activity of phenylpropanoyl esters of catechol glycoside and its dimers from Dodecadenia grandiflora

Author:
Kumar, Manmeet; Rawat, Preeti; Rahuja, Neha; Srivastava, Arvind Kumar; Maurya, Rakesh
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1448-1455
ISSN:
0031-9422
Subject:
Laurales; esters; catechol; glycosides; lignans; acetylation; medicinal properties; glycemic effect; chemical structure; spectral analysis; acid hydrolysis; glucose tolerance; diabetes mellitus
Abstract:
... Bioactivity-guided separation of an antihyperglycemic extract from the leaves of Dodecadenia grandiflora afforded two phenylpropanoyl esters of catechol glycosides (1 and 4) and two lignane bis(catecol glycoside)esters (2 and 3). Their structures were established on the basis of extensive spectroscopic analysis (1D and 2D-NMR, MS). Compounds 2 and 3 are believed to be derived from dimerization via ...
DOI:
10.1016/j.phytochem.2009.07.029
PubMed:
19700178

4. Biotransformation of 20(S)-protopanaxadiol by Mucor spinosus

Author:
Li, Huifeng; Guo, Hongzhu; Tian, Yin; Zhang, Jie; Zhou, Jianping; Hu, Yuchi; Guo, Dean
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1416-1420
ISSN:
0031-9422
Subject:
Mucor; triterpenoids; phytochemicals; biotransformation; cytotoxicity; anticarcinogenic activity; humans
Abstract:
... Biotransformation of 20(S)-protopanaxadiol (1) by the fungus Mucor spinosus AS 3.3450 yielded eight metabolites (2-9). On the basis of NMR and MS analyses, the metabolites were identified as 12-oxo-15α,27-dihydroxyl-20(S)-protopanaxadiol (2), 12-oxo-7β,11α,28-trihydroxyl-20(S)-protopanaxadiol (3), 12-oxo-7β,28-dihydroxyl-20(S)-protopanaxadiol (4), 12-oxo-15α,29-dihydroxyl-20(S)-protopanaxadiol (5) ...
DOI:
10.1016/j.phytochem.2009.07.041
PubMed:
19732918

5. Characterization of a highly stable trypsin-like proteinase inhibitor from the seeds of Opuntia streptacantha (O. streptacantha Lemaire)

Author:
Torres-Castillo, J.A.; Mondragon Jacobo, C.; Blanco-Labra, A.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1374-1381
ISSN:
0031-9422
Subject:
insect pests; trypsin inhibitors; Opuntia ficus-indica; isoelectric point; pest control; Periplaneta americana; spectral analysis; Acheta; Gryllus; enzyme activity; Prostephanus truncatus; plant proteins
Abstract:
... A trypsin inhibitor from Opuntia streptacantha Lemaire (Prickly pear) seeds was purified and characterized. Of several proteases tested, this inhibitor showed specificity to trypsin-like enzymes. The major inhibitor present in these seeds showed distinctive characteristics, most notably a low molecular weight of 4.19 kDa, as determined by MALDI TOF, and an unusually high thermal stability, retaini ...
DOI:
10.1016/j.phytochem.2009.08.009
PubMed:
19765785

6. Cytotoxic ent-kaurane diterpenoids from Isodon sinuolata

Author:
He, Fei; Xiao, Wei-Lie; Pu, Jian-Xin; Wu, Ying-Li; Zhang, Hai-Bo; Li, Xiao-Nian; Zhao, Yong; Yang, Li-Bin; Chen, Guo-Qiang; Sun, Han-Dong
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1462-1466
ISSN:
0031-9422
Subject:
humans; phytochemicals; cytotoxicity; medicinal plants; diterpenoids; Isodon (Lamiaceae); spectral analysis; cell proliferation; chemical structure; stereochemistry; anticarcinogenic activity; China
Abstract:
... Five (1-5) ent-kaurane diterpenoids and 17 other known ones, were isolated from the leaves and stems of Isodon sinuolata. Their structures were determined on the basis of spectroscopic methods including 1D and 2D NMR spectroscopic analysis. All compounds were evaluated for cytotoxicity against a small panel of cell lines. Some compounds exhibited significant cytotoxicity. ...
DOI:
10.1016/j.phytochem.2009.07.037
PubMed:
19712947

7. Determination of β-glucosidase aggregating factor (BGAF) binding and polymerization regions on the maize β-glucosidase isozyme Glu1

Author:
Yu, Hyun Young; Kittur, Farooqahmed S.; Bevan, David R.; Esen, Asim
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1355-1365
ISSN:
0031-9422
Subject:
Zea mays; corn; beta-glucosidase; isozymes; molecular cloning; gene expression regulation; lectins
Abstract:
... β-Glucosidases (Glu1 and Glu2) in maize specifically interact with a lectin called β-glucosidase aggregating factor (BGAF). We have shown that the N-terminal (Glu50-Val145) and the C-terminal (Phe466-Ala512) regions of maize Glu1 are involved in binding to BGAF. Sequence comparison between sorghum β-glucosidases (dhurrinases, which do not bind to BGAF) and maize β-glucosidases, and the 3D-structur ...
DOI:
10.1016/j.phytochem.2009.07.026

8. Differing mechanisms of simple nitrile formation on glucosinolate degradation in Lepidium sativum and Nasturtium officinale seeds

Author:
Williams, David J.; Critchley, Christa; Pun, Sharon; Chaliha, Mridusmita; O'Hare, Timothy J.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1401-1409
ISSN:
0031-9422
Subject:
Lepidium sativum; Nasturtium officinale; seeds; nitriles; glucosinolates; thioglucosidase; enzymatic hydrolysis; biochemical pathways; thermal degradation; isothiocyanates; chelating agents; EDTA (chelating agent); deferoxamine
Abstract:
... Glucosinolates are sulphur-containing glycosides found in brassicaceous plants that can be hydrolysed enzymatically by plant myrosinase or non-enzymatically to form primarily isothiocyanates and/or simple nitriles. From a human health perspective, isothiocyanates are quite important because they are major inducers of carcinogen-detoxifying enzymes. Two of the most potent inducers are benzyl isothi ...
DOI:
10.1016/j.phytochem.2009.07.035
PubMed:
19747700

9. Hemiterpene glucosides and other constituents from Spiraea canescens

Author:
Choudhary, M. Iqbal; Naheed, Nadra; Abbaskhan, Ahmed; Ali, Sajjad; Atta-ur-Rahman
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1467-1473
ISSN:
0031-9422
Subject:
Spiraea; medicinal plants; Oriental traditional medicine; phytochemicals; terpenoids; glucosides; chemical structure; spectral analysis; medicinal properties; antioxidant activity
Abstract:
... Five glycosides, 2-(trans-cinnamoyloxy-methyl)-1-butene-4-O-β-d-glucopyranoside (1), 4-(6'-O-trans-cinnamoyl)-(2-hydroxymethyl-4-hydroxy-butenyl-β-d-glucopyranoside (2), 6''-O-trans-p-coumaroyl-(4-hydroxybenzoyl)-β-d-glucopyranoside (3), 6'-O-(4-methoxy-trans-cinnamoyl) α/β-d-glucopyranose (4) 6'-O-(4''-methoxy-trans-cinnamoyl)-kaempferol-3-β-d-glucopyranoside (7) along with six known compounds, ( ...
DOI:
10.1016/j.phytochem.2009.07.013
PubMed:
19747701

10. Identification, recombinant expression, and biochemical characterization of a flavonol 3-O-glucosyltransferase clone from Citrus paradisi

Author:
Owens, Daniel K.; McIntosh, Cecilia A.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1382-1391
ISSN:
0031-9422
Subject:
Citrus paradisi; grapefruits; glycosyltransferases; molecular cloning; gene expression regulation; recombinant fusion proteins; glycosylation; structure-activity relationships; enzyme activity; substrate specificity; buffers; enzyme kinetics; enzyme inhibitors; thermal stability; amino acid sequences
Abstract:
... Glucosylation is a predominant flavonoid modification reaction affecting the solubility, stability, and subsequent bioavailability of these metabolites. Flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications in these species. In this work, a Citrus paradisi glucosyltransferase gene was i ...
DOI:
10.1016/j.phytochem.2009.07.027
PubMed:
19733370

11. Monoclonal antibodies to p-coumarate

Author:
Tranquet, Olivier; Saulnier, Luc; Utille, Jean-Pierre; Ralph, John; Guillon, Fabienne
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1366-1373
ISSN:
0031-9422
Subject:
antibody formation; cell walls; tissue distribution; wheat; lignification; monoclonal antibodies; aleurone layer; p-coumaric acid; esterification; esters; Triticum aestivum; oligosaccharides; ferulic acid
Abstract:
... p-Coumaric acid is one of the predominant phenolic acids acylating the cell walls of grasses; p-coumarates are mainly esterified by lignins and arabinoxylans. Here we describe the production and characterisation of two monoclonal antibodies against p-coumarates. The 5-O-pCou-Ara(1 leads to 4)Xyl was chemically synthesized and conjugated to a carrier protein. Two interesting antibodies were obtaine ...
DOI:
10.1016/j.phytochem.2009.06.019
PubMed:
19712948

12. Mycorrhization alleviates benzo[a]pyrene-induced oxidative stress in an in vitro chicory root model

Author:
Debiane, Djourher; Garcon, Guillaume; Verdin, Anthony; Fontaine, Joel; Durand, Roger; Shirali, Pirouz; Grandmougin-Ferjani, Anne; Sahraoui, Anissa Lounes-Hadj
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1421-1427
ISSN:
0031-9422
Subject:
pyrene (hydrocarbon); chicory; vesicular arbuscular mycorrhizae; root systems; mycorrhizal fungi; oxidative stress; lipid peroxidation; superoxide dismutase; pollutants; root growth; Rhizophagus intraradices; enzyme activity; antioxidant activity; Cichorium intybus
Abstract:
... Among chemicals that are widely spread both in terrestrial and aquatic ecosystems, benzo[a]pyrene is a major source of concern. However, little is known about its adverse effects on plants, as well as about the role of mycorrhization in protection of plant grown in benzo[a]pyrene-polluted conditions. Hence, to contribute to a better understanding of the adverse effects of polycyclic aromatic hydro ...
DOI:
10.1016/j.phytochem.2009.07.002
PubMed:
19758666

13. Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties

Author:
Andersson, Derek; Chakrabarty, Romit; Bejai, Sarosh; Zhang, Jiaming; Rask, Lars; Meijer, Johan
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1345-1354
ISSN:
0031-9422
Subject:
Arabidopsis thaliana; gene overexpression; recombinant fusion proteins; amino acid sequences; molecular cloning; thioglucosidase; enzyme kinetics; enzyme activity; isozymes
Abstract:
... Myrosinases (EC 3.2.1.147) are β-thioglucoside glucosidases present in Brassicaceae plants. These enzymes serve to protect plants against pathogens and insect pests by initiating breakdown of the secondary metabolites glucosinolates into toxic products. Several forms of myrosinases are present in plants but the properties and role of different isoenzymes are not well understood. The dicot plant mo ...
DOI:
10.1016/j.phytochem.2009.07.036
PubMed:
19703694

14. Oxidative burst inhibitory and cytotoxic amides and lignans from the stem bark of Fagara heitzii (Rutaceae)

Author:
Mbaze, Luc Meva'a; Lado, Jean Alexandre; Wansi, Jean Duplex; Shiao, Tze Chieh; Chiozem, David Dako; Mesaik, Muhammad Ahmed; Choudhary, Muhammad Iqbal; Lacaille-Dubois, Marie-Aleth; Wandji, Jean; Roy, Rene; Sewald, Norbert
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1442-1447
ISSN:
0031-9422
Subject:
Zanthoxylum; bark; amides; lignans; phytochemicals; chemical structure; spectral analysis; antioxidant activity; cytotoxicity; anticarcinogenic activity
Abstract:
... Two amides, heitziamide A and heitziamide B and two phenylethanoids, heitziethanoid A and heitziethanoid B together with thirteen known compounds were isolated from F. heitzii (Letouzey). The structures of all compounds were established by spectroscopic analysis. Nine compounds were evaluated for oxidative burst inhibitory activity in a chemoluminescence assay and for cytotoxicity against PC-3 pro ...
DOI:
10.1016/j.phytochem.2009.08.007
PubMed:
19747699

15. Phenylpropanoid polyamine conjugate biosynthesis in Arabidopsis thaliana flower buds

Author:
Fellenberg, Christin; Bottcher, Christoph; Vogt, Thomas
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1392-1400
ISSN:
0031-9422
Subject:
Arabidopsis thaliana; messenger RNA; buds; phenylpropanoids; spermine; flowers; transcription (genetics); gene expression regulation; acyltransferases
Abstract:
... Phenylpropanoid polyamine conjugates have been identified in flowers of many plant species. Their presence in Arabidopsis thaliana has only been recently established in flower buds and pollen grains. Annotation and location of a cation-dependent O-methyltransferase AtTSM1 specifically to the tapetum of young flower buds enabled the subsequent identification of several genes with a putative role in ...
DOI:
.1016/j.phytochem.2009.08.010

16. Rare prenylated flavonoids from Tephrosia purpurea

Author:
Hegazy, Mohamed-Elamir F.; Abd El-Razek, Mohamed H.; Nagashima, Fumihiro; Asakawa, Yoshinori; Pare, Paul W.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1474-1477
ISSN:
0031-9422
Subject:
Tephrosia purpurea; medicinal plants; chemical constituents of plants; flavonoids; chemical structure; spectral analysis; stereochemistry; Egypt
Abstract:
... Chemical investigations of aerial parts of Tephrosia purpurea yielded the rare prenylated flavonoids, tephropurpulin A (1) and isoglabratephrin (2), in addition to a previously identified flavonoid, glabratephrin (3). Structures were established by 1D and 2D NMR spectroscopy, as well as by HR-MS analysis; for compounds 2 and 3, structures were confirmed by X-ray analysis. ...
DOI:
10.1016/j.phytochem.2009.08.001
PubMed:
19733371

17. Regulation of malate metabolism in grape berry and other developing fruits

Author:
Sweetman, Crystal; Deluc, Laurent G.; Cramer, Grant R.; Ford, Christopher M.; Soole, Kathleen L.
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1329-1344
ISSN:
0031-9422
Subject:
genetic transformation; malic enzyme; grapes; malate dehydrogenase; phosphoenolpyruvate carboxylase; glyoxylate cycle; alcoholic fermentation; physiological transport; gluconeogenesis; cell respiration; malic acid; glycolysis; photosynthesis; lactic fermentation; fruiting; tricarboxylic acid cycle
Abstract:
... Organic acids are present in all plants, supporting numerous and varied facets of cellular metabolism. The type of organic acid found, and the levels to which they accumulate are extremely variable between species, developmental stages and tissue types. Acidity plays important roles in the organoleptic properties of plant tissues, where examples of both enhanced and reduced palatability can be asc ...
DOI:
10.1016/j.phytochem.2009.08.006
PubMed:
19762054

18. Sesquiterpenoids from Teucrium ramosissimum

Author:
Henchiri, Hichem; Bodo, Bernard; Deville, Alexandre; Dubost, Lionel; Zourgui, Lazhar; Raies, Aly; Grellier, Philippe; Mambu, Lengo
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1435-1441
ISSN:
0031-9422
Subject:
Teucrium; medicinal plants; phytochemicals; sesquiterpenoids; Plasmodium falciparum; humans; cultured cells; cytotoxicity; anticarcinogenic activity; chemical structure; spectral analysis
Abstract:
... An antiplasmodial bioguided investigation of the EtOAc extract of the aerial parts of Teucrium ramosissimum led to isolation and identification of three sesquiterpenoids, teucmosin, 4α-hydroxy-homalomenol C, 1β,4β,7α-trihydroxy-8,9-eudesmene and two trinorsesquiterpenoids, 4β-hydroxy-11,12,13-trinor-5-eudesmen-1,7-dione and 1β,4β-dihydroxy-11,12,13-trinor-8,9-eudesmen-7-one together with five know ...
DOI:
10.1016/j.phytochem.2009.08.012
PubMed:
19766274

19. Xanthones from Hypericum chinense and their cytotoxicity evaluation

Author:
Tanaka, Naonobu; Kim, Sang-Yong; Sekiya, Michiko; Ikeshiro, Yasumasa; Takaishi, Yoshihisa
Source:
Phytochemistry 2009 v.70 no.11-12 pp. 1456-1461
ISSN:
0031-9422
Subject:
Hypericum; Clusiaceae; xanthones; chemical structure; spectral analysis; stereochemistry; cytotoxicity; anticarcinogenic activity; cultured cells; humans
Abstract:
... A xanthonolignoid, 2-O-demethylkielcorin, and a phenylxanthone, chinexanthone A, were isolated from stems of Hypericum chinense together with four known xanthonolignoids and seven known xanthones. Their structures were established by spectroscopic analysis, as their optical properties and absolute stereochemistry determined. The cytotoxicities of the isolated xanthone derivatives as well as additi ...
DOI:
10.1016/j.phytochem.2009.08.015
PubMed:
19766276