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- Maiale, Santiago J.; Marina, Maria; Sanchez, Diego H.; Pieckenstain, Fernando L.; Ruiz, Oscar A.
- Phytochemistry 2008 v.69 no.14 pp. 2552-2558
- Avena sativa; oats; Zea mays; corn; polyamines; oxidoreductases; enzyme inhibition; enzyme activity; enzyme kinetics; substrate specificity; antibacterial properties; Pseudomonas viridiflava; antifungal properties; Sclerotinia sclerotiorum; diamines
- ... Polyamine oxidase from Avena sativa L. cv. Cristal seedlings was purified to homogeneity using a simple four-step purification protocol including an infiltration washing technique. The enzyme had a high affinity for spermidine and spermine (K(m) approximately 5.5 and 1.2 μM, respectively), and also oxidized norspermidine (K(m) approximately 64.0 μM). Natural and synthetic diamines, cyclohexylamine ...