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You searched for: Journal Plant cell, tissue, and organ culture Remove constraint Journal: Plant cell, tissue, and organ culture Publication Year 2020 Remove constraint Publication Year: 2020 Subject culture media Remove constraint Subject: culture media Subject explants Remove constraint Subject: explants
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1. Production of verbascoside and its analogues in in vitro cultures of Verbascum thapsus L.

Author:
Temporiti, Marta Elisabetta Eleonora; Frezza, Claudio; Beccaccioli, Marzia; Gelardi, Luca; Bianco, Armandodoriano; Bonina, Francesco Paolo; Nielsen, Erik
Source:
Plant cell, tissue, and organ culture 2020 v.140 no.1 pp. 83-93
ISSN:
0167-6857
Subject:
Verbascum thapsus; callus; culture media; cultured cells; explants; glycosides; leaf extracts; leaves; phytochemicals; tissue culture; verbascoside
Abstract:
... In this work an effective method to start axenic cultures of Verbascum thapsus L. was developed. This allowed to obtain a high percentage of callus generation from leaf explants (around 70%) and to start the cultivation of suspension cultured cells rapidly growing in liquid culture medium. Moreover, the production in these materials of the phenylethanoid glycoside compound verbascoside was verifie ...
DOI:
10.1007/s11240-019-01712-5

2. A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

Author:
Fan, Yinglun; Xu, Fenglin; Zhou, Huizhen; Liu, Xinxin; Yang, Xinyue; Weng, Kaixia; Sun, Xinlu; Lyu, Shanhua
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.1 pp. 207-216
ISSN:
0167-6857
Subject:
Agrobacterium; Arabidopsis; Cucumis sativus; Rhizobium rhizogenes; cucumbers; culture media; explants; genes; hypocotyls; root tips; seedlings; vermiculite
Abstract:
... Agrobacterium rhizogenes-mediated transformation is widely used in different species with various purposes. The development of composite plants (wild-type shoot with transgenic roots) has been a milestone for functional characterization of genes. Previously, composite plants were generated by two steps from inducing of hairy roots to growing in the growth medium. Hairy roots were induced in an ind ...
DOI:
10.1007/s11240-020-01781-x

3. A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals

Author:
Dalton, S. J.
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 257-273
ISSN:
0167-6857
Subject:
Avena sativa; Brachypodium distachyon; Deschampsia cespitosa; Festuca arundinacea; Lolium perenne; Lolium temulentum; Miscanthus sinensis; Oryza sativa; Phalaris arundinacea; bioenergy; callus; copper sulfate; culture media; embryogenesis; explants; forage; genetic transformation; genotype; glutamine; grains; magnesium sulfate; meristems; models; morphogenesis; new species; potassium phosphates; proline; recalcitrant species; shoot tips; tropical grasses
Abstract:
... Slow callus growth is a barrier to efficient genetic transformation in some gramineous species. A reformulation of Murashige and Skoog (MS) medium, with additional magnesium sulphate, potassium phosphate, copper sulphate, proline and glutamine, termed WPBS medium, has been developed which improves all aspects of in vitro culture when compared with MS based media. Embryogenic callus could be produc ...
DOI:
10.1007/s11240-020-01784-8
PubMed:
32308245
PubMed Central:
PMC7145791

4. Use of Sorghum bicolor leaf whorl explants to expedite regeneration and increase transformation throughput

Author:
Silva, Tallyta N.; Kelly, Megan E.; Vermerris, Wilfred
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 243-255
ISSN:
0167-6857
Subject:
DNA; Sorghum bicolor; absorbance; activated carbon; biolistics; callus; color; culture media; explants; gene editing; genotype; gold; greenhouses; growth chambers; leaves; polyvinylpyrrolidone; seedlings; somatic embryos; thin layer chromatography; tissue culture; transgenes
Abstract:
... Sorghum is considered a challenging species for transformation due to the limited choice in genotypes that can be transformed and the reliance on callus derived from immature embryos that need to be teased out of developing seeds. We have developed a more practical tissue culture procedure to generate embryogenic callus from leaf whorl explants obtained from 4 week-old seedlings. This procedure of ...
DOI:
10.1007/s11240-020-01783-9

5. In vitro micropropagation of Basilicum polystachyon (L.) Moench and identification of endogenous auxin through HPLC

Author:
Das, Sumanta; Sultana, Kaniz Wahida; Chandra, Indrani
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.3 pp. 633-641
ISSN:
0167-6857
Subject:
acclimation; culture media; explants; indole acetic acid; indole butyric acid; micropropagation; naphthaleneacetic acid; reversed-phase high performance liquid chromatography; root tips; rooting; shoot tips; survival rate
Abstract:
... An efficient plant regeneration protocol was developed for Basilicum polystachyon (L.) Moench using shoot tip from in vitro germinated plant. Both shoot multiplication and root induction were initiated from shoot tip explants in Murashige and Skoog’s (MS) basal medium supplemented with N⁶-benzylaminopurine (BAP) and 6-furfurylaminopurine (Kin) combination with 1-naphthaleneacetic acid (NAA) and wi ...
DOI:
10.1007/s11240-020-01824-3

6. Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses

Author:
Ganguly, Shreeparna; Ghosh, Gourab; Ghosh, Sanatan; Purohit, Arnab; Chaudhuri, Rituparna Kundu; Das, Sampa; Chakraborti, Dipankar
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.3 pp. 493-504
ISSN:
0167-6857
Subject:
Agrobacterium radiobacter; Southern blotting; abiotic stress; agronomic traits; benzyladenine; biotic stress; chickpeas; coculture; cultivars; culture media; explants; genetic improvement; genetic transformation; genomics; genotype; kanamycin; meristems; mutants; naphthaleneacetic acid; polymerase chain reaction; rooting; screening; seedlings; stress tolerance; tissue culture; transgenes; transgenic plants
Abstract:
... Major biotic and abiotic stresses have led to the reduction of chickpea productivity, creating a strong barrier for its utilization as major food legume. Genetic transformation has revolutionized the chickpea improvement programs for biotic stress tolerance. Recent knowledge on genomic resources offers a range of approaches for genetic improvement of chickpea with a greater resolution, but relies ...
DOI:
10.1007/s11240-020-01873-8