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1. Nuclear DNA content as an indicator of inflorescence colour stability of in vitro propagated solid and chimera mutants of chrysanthemum

Author:
Miler, Natalia; Kulus, Dariusz; Sliwinska, Elwira
Source:
Plant cell, tissue, and organ culture 2020 v.143 no.2 pp. 421-430
ISSN:
0167-6857
Subject:
Chrysanthemum; DNA; X-radiation; acclimation; benzyladenine; chimerism; color; cultivars; explants; flow cytometry; flowers; gamma radiation; greenhouses; indole acetic acid; mutants; nuclear genome; phenotype; plantlets; shoots
Abstract:
... In chrysanthemum, breeders seek for desirable characteristics of the inflorescence, which can first be established once the plant is mature. The present study aims to determine whether measurement of DNA content can be useful in the detection of somaclonal variants and/or separation of chimera components in chrysanthemum at the early in vitro multiplication stage. Eleven Chrysanthemum × morifolium ...
DOI:
10.1007/s11240-020-01929-9

2. Temporary immersion systems to improve alder micropropagation

Author:
San José, M. C.; Blázquez, N.; Cernadas, M. J.; Janeiro, L. V.; Cuenca, B.; Sánchez, C.; Vidal, N.
Source:
Plant cell, tissue, and organ culture 2020 v.143 no.2 pp. 265-275
ISSN:
0167-6857
Subject:
Alnus glutinosa; acclimation; benzyladenine; bioreactors; explants; hyperhydricity; indole acetic acid; indole butyric acid; liquids; micropropagation; rooting; shoots; woody plants
Abstract:
... A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l⁻ ¹ benzyladenine and 0.5 mg l⁻ ¹ indole acetic acid. The effect of the bioreactor type (RITA® and Plantform™), frequency of the immersions, regulator concentrations, and volume of medium per explant was investigated. All ...
DOI:
10.1007/s11240-020-01937-9

3. High-efficiency somatic embryogenesis techniques for different hybrids of cut lilies

Author:
Yan, Rui; Wang, Chunxia; Wang, Jinxia; Nie, Ruxia; Sun, Hongmei
Source:
Plant cell, tissue, and organ culture 2020 v.143 no.1 pp. 145-157
ISSN:
0167-6857
Subject:
Lilium longiflorum; cotyledons; explants; germination; germplasm; hybrids; in vitro regeneration; leaves; naphthaleneacetic acid; ornamental plants; picloram; plant breeding; plant reproduction; seedlings; solar radiation; somatic embryogenesis; somatic embryos
Abstract:
... Somatic embryogenesis (SE) is a unique method of in vitro regeneration for plant reproduction, germplasm resource preservation, and molecular-assisted breeding. Although lily is an internationally valued ornamental plant, its SE technology is still incomplete. Here, the effects of different explants, picloram (PIC) concentrations, and light conditions on somatic embryo induction, development, germ ...
DOI:
10.1007/s11240-020-01904-4

4. Indirect regeneration of Ficus carica by the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR

Author:
Abdolinejad, Ruhollah; Shekafandeh, Akhtar; Jowkar, Abolfazl; Gharaghani, Ali; Alemzadeh, Abbas
Source:
Plant cell, tissue, and organ culture 2020 v.143 no.1 pp. 131-144
ISSN:
0167-6857
Subject:
Ficus carica; breeding programs; callus; cultivars; explants; figs; flow cytometry; fruit growing; fruit trees; genetic markers; indole butyric acid; mature plants; microsatellite repeats; morphogenesis; naphthaleneacetic acid; nutrition; plant breeding; ploidy; shoots
Abstract:
... The fig (Ficus carica L.), is known as a precious fruit tree for its nutrition and medicinal values, economic importance and for sustainable production in the semi-arid and arid areas. Expanding the cultivation of fig in new vulnerable areas and the breeding programs in fig need a reliable high-efficient system for in vitro morphogenesis to meet future demands. This study was carried to develop an ...
DOI:
10.1007/s11240-020-01903-5

5. Pigment elicitation and sun protection factor of callus induced from Cassia tora seedling explants

Author:
Shah, Mansi; George, Indu Anna
Source:
Plant cell, tissue, and organ culture 2020 v.143 no.1 pp. 201-210
ISSN:
0167-6857
Subject:
Senna tora; anthraquinones; benzyladenine; callus; callus culture; elicitors; ethephon; explants; fungi; insects; medicinal properties; pigments; plant extracts; salicylic acid; seedlings; textile industry; thidiazuron
Abstract:
... Natural pigments are derived from sources such as plant, animal, insects, and fungi. The anthraquinones are natural pigments that have pharmacological activities. They have applications in several domains such as the pharmaceutical, cosmetic and textile industries. Anthraquinones occur naturally in many plants including Cassia tora of the Fabaceae family. The present study focused on the productio ...
DOI:
10.1007/s11240-020-01913-3

6. An efficient protocol for Agrobacterium-mediated genetic transformation of Antirrhinum majus

Author:
Lian, Zhaoyuan; Nguyen, Chi D.; Wilson, Sandra; Chen, Jianjun; Gong, Haijun; Huo, Heqiang
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.3 pp. 527-536
ISSN:
0167-6857
Subject:
Antirrhinum majus; CRISPR-Cas systems; adventitious shoots; cotyledons; explants; gene editing; genetic transformation; genome; hypocotyls; models; naphthaleneacetic acid; organogenesis; petioles; plant breeding; seedlings; silver nitrate; zeatin
Abstract:
... Snapdragon (Antirrhinum majus L.) is a popular ornamental and model plant species, and the recently released reference genome could greatly boost its utilization in fundamental research. However, the lack of an efficient genetic transformation system is still a major limiting factor for its full application in genetic and molecular studies. In this study, a simple method for quick regeneration and ...
DOI:
10.1007/s11240-020-01877-4

7. Culture selected somaclonal variants showing low-ODAP and high protein content in nineteen grass pea (Lathyrus sativus L.) genotypes

Author:
Barpete, Surendra; Gupta, Priyanka; Singh, Murari; Kumar, Shiv
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.3 pp. 625-634
ISSN:
0167-6857
Subject:
Lathyrus sativus; agronomic traits; benzyladenine; explants; genotype; heritability; naphthaleneacetic acid; parents; protein content; somaclonal variation; tissue culture
Abstract:
... To develop low-ODAP grass pea genotypes with high protein content, in vitro tissue culture techniques were used for inducing somaclonal variation through internode explants that was cultured on B5 medium containing 2 mg L⁻ ˡ α-naphthaleneacetic acid and 1 mg L⁻ ˡ 6-benzylaminopurine of 19 grass pea genotypes. The selected somaclones (R₃ and R₄ generations) were evaluated for variation in ODAP, pro ...
DOI:
10.1007/s11240-020-01889-0

8. Genetic analysis of callus formation in a diversity panel of 96 rose genotypes

Author:
Nguyen, Thi Hong Nhung; Winkelmann, Traud; Debener, Thomas
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.3 pp. 505-517
ISSN:
0167-6857
Subject:
acetic acid; alleles; binding proteins; callus; callus formation; chromosomes; explants; genetic analysis; genetic factors; genome-wide association study; genotype; hydrolases; leaves; phenotypic variation; plant growth substances; single nucleotide polymorphism; single nucleotide polymorphism arrays
Abstract:
... In a diversity panel of 96 rose genotypes, variation in the capacity to form calluses on leaf explants in vitro was investigated, and a genome-wide association study (GWAS) was performed to identify genetic factors associated with callus formation. Calluses were induced from wounded in vitro leaflets on two media differing in their plant growth regulator composition. Significant differences betwee ...
DOI:
10.1007/s11240-020-01875-6

9. Plumular meristem transformation system for chickpea: an efficient method to overcome recalcitrant tissue culture responses

Author:
Ganguly, Shreeparna; Ghosh, Gourab; Ghosh, Sanatan; Purohit, Arnab; Chaudhuri, Rituparna Kundu; Das, Sampa; Chakraborti, Dipankar
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.3 pp. 493-504
ISSN:
0167-6857
Subject:
Agrobacterium radiobacter; Southern blotting; abiotic stress; agronomic traits; benzyladenine; biotic stress; chickpeas; coculture; cultivars; culture media; explants; genetic improvement; genetic transformation; genomics; genotype; kanamycin; meristems; mutants; naphthaleneacetic acid; polymerase chain reaction; rooting; screening; seedlings; stress tolerance; tissue culture; transgenes; transgenic plants
Abstract:
... Major biotic and abiotic stresses have led to the reduction of chickpea productivity, creating a strong barrier for its utilization as major food legume. Genetic transformation has revolutionized the chickpea improvement programs for biotic stress tolerance. Recent knowledge on genomic resources offers a range of approaches for genetic improvement of chickpea with a greater resolution, but relies ...
DOI:
10.1007/s11240-020-01873-8

10. Induction of somatic embryogenesis in different explants from Ormosia henryi Prain

Author:
Wu, Gao-Yin; Wei, Xiao-Li; Wang, Xiao; Wei, Yi
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.2 pp. 229-240
ISSN:
0167-6857
Subject:
2,4-D; Ormosia; benzyladenine; callus; cotyledons; explants; germination; histology; hypocotyls; kinetin; leaves; naphthaleneacetic acid; root meristems; shoots; somatic embryogenesis; somatic embryos; thidiazuron; trees; wood industry
Abstract:
... An efficient in vitro plant regeneration method via somatic embryogenesis has been established in Ormosia henryi Prain, which is an important precious tree in the timber industry. Five types of explants, six types of basic media and different plant growth regulators (PGRs) were used for embryogenic callus (EC) initiation, EC proliferation, somatic embryo (SE) induction and SE germination. The EC a ...
DOI:
10.1007/s11240-020-01822-5

11. Efficient plant regeneration from in vitro leaves and petioles via shoot organogenesis in Sapium sebiferum Roxb.

Author:
Hou, Jinyan; Su, Pengfei; Wang, Dacheng; Chen, Xue; Zhao, Weiwei; Wu, Lifang
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.1 pp. 143-156
ISSN:
0167-6857
Subject:
Triadica sebifera; acclimation; adventitious shoots; callus; explants; genetic improvement; genetic markers; greenhouses; indole acetic acid; indole butyric acid; mechanical damage; microsatellite repeats; multipurpose trees; naphthaleneacetic acid; organogenesis; petioles; plantlets; survival rate; thidiazuron
Abstract:
... Sapium sebiferum Roxb. is a widespread and economically important multipurpose tree due to its high value in ornamental, and biodiesel production as well as medicine. A highly efficient in vitro plant regeneration system through direct shoot organogenesis was established for the first time from leaves and petioles of S. sebiferum. The results showed that plant growth regulators (PGRs), mechanical ...
DOI:
10.1007/s11240-020-01848-9

12. Micropropagation protocol for coastal quinoa

Author:
Regalado, J. J.; Tossi, V. E.; Burrieza, H. P.; Encina, C. L.; Pitta-Alvarez, S. I.
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.1 pp. 213-219
ISSN:
0167-6857
Subject:
abiotic stress; acclimation; breeding programs; cultivars; explants; fatty acids; halophytes; indole butyric acid; micropropagation; models; plant breeding; prolamins; rooting; seeds; shoots; stress tolerance; warm season
Abstract:
... Quinoa is a model halophyte plant, with seeds rich in proteins, fatty acids and lacking prolamines. There are two types of quinoa cultivars: highland and coastal. Coastal cultivars are used in breeding programs for warm-season quinoa production. Different biotechnological tools are useful for breeding and understanding the mechanisms involved in abiotic stress tolerance. However, micropropagation ...
DOI:
10.1007/s11240-020-01840-3

13. The comparative analysis of stilbene production in cell cultures of two East Asian grape species Vitis amurensis Rupr. and Vitis coignetiae Pulliat

Author:
Tyunin, Alexey P.; Nityagovsky, Nikolay N.; Karetin, Yuri A.; Kiselev, Konstantin V.
Source:
Plant cell, tissue, and organ culture 2020 v.142 no.1 pp. 121-129
ISSN:
0167-6857
Subject:
Vitis amurensis; Vitis coignetiae; bioactive properties; biological models; biosynthesis; cell culture; drought tolerance; explants; genes; grapes; petioles; resveratrol; secondary metabolites; transcription factors; trihydroxystilbene synthase
Abstract:
... Two East Asian grape species Vitis amurensis and Vitis coignetiae have been investigated for their increased cold and drought tolerance and high level of resistance to infections. Nowadays, the grape species attract attention due to their capability to produce a group of valuable plant secondary metabolites named stilbenes. The basic stilbene, t-resveratrol, is known to demonstrate a high bioactiv ...
DOI:
10.1007/s11240-020-01845-y

14. High efficiency plant regeneration and genetic fidelity of regenerants by SCoT and ISSR markers in chickpea (Cicer arietinum L.)

Author:
Sadhu, SumanKalyan; Jogam, Phanikanth; Thampu, Raja Komuraiah; Abbagani, Sadanandam; Penna, Suprasanna; Peddaboina, Venkataiah
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.3 pp. 465-477
ISSN:
0167-6857
Subject:
Cicer arietinum; acclimation; benzyladenine; chickpeas; explants; genetic markers; genetic transformation; gibberellic acid; greenhouses; in vitro regeneration; indole acetic acid; indole butyric acid; kinetin; meristems; microsatellite repeats; plantlets; rooting; seeds; shoots; start codon; survival rate; thidiazuron
Abstract:
... High efficient and repeatable in vitro regeneration protocol was established from embryo axis, half-seed, axillary meristem, and cotyledonary node explants of chickpea. Various concentrations and combinations of various plant growth regulators (PGRs) were employed to induce multiple shoots, shoot elongation and rooting of shoots to obtain complete plantlets of chickpea. The pretreatment of seeds w ...
DOI:
10.1007/s11240-020-01804-7

15. In vitro micropropagation of Basilicum polystachyon (L.) Moench and identification of endogenous auxin through HPLC

Author:
Das, Sumanta; Sultana, Kaniz Wahida; Chandra, Indrani
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.3 pp. 633-641
ISSN:
0167-6857
Subject:
acclimation; culture media; explants; indole acetic acid; indole butyric acid; micropropagation; naphthaleneacetic acid; reversed-phase high performance liquid chromatography; root tips; rooting; shoot tips; survival rate
Abstract:
... An efficient plant regeneration protocol was developed for Basilicum polystachyon (L.) Moench using shoot tip from in vitro germinated plant. Both shoot multiplication and root induction were initiated from shoot tip explants in Murashige and Skoog’s (MS) basal medium supplemented with N⁶-benzylaminopurine (BAP) and 6-furfurylaminopurine (Kin) combination with 1-naphthaleneacetic acid (NAA) and wi ...
DOI:
10.1007/s11240-020-01824-3

16. Silver compounds regulate leaf drop and improve in vitro regeneration from mature tissues of Australian finger lime (Citrus australasica)

Author:
Mahmoud, Lamiaa M.; Grosser, Jude W.; Dutt, Manjul
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.3 pp. 455-464
ISSN:
0167-6857
Subject:
Citrus; Microcitrus australasica; breeding programs; ethylene production; explants; gibberellic acid; in vitro regeneration; leaf abscission; leaves; micropropagation; nanosilver; naphthaleneacetic acid; seeds; shoots; silver nitrate; silver thiosulfate; tissue culture; Australia
Abstract:
... Finger lime is a small-leaved citrus native to Australia that is becoming increasingly popular worldwide. Finger lime is monoembryonic and cannot be propagated true to type from seeds. Initial studies to develop a tissue culture protocol for the mass production of elite lines being developed in our breeding program were unsuccessful due to complete leaf abscission during the culture establishment ...
DOI:
10.1007/s11240-020-01803-8

17. A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals

Author:
Dalton, S. J.
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 257-273
ISSN:
0167-6857
Subject:
Avena sativa; Brachypodium distachyon; Deschampsia cespitosa; Festuca arundinacea; Lolium perenne; Lolium temulentum; Miscanthus sinensis; Oryza sativa; Phalaris arundinacea; bioenergy; callus; copper sulfate; culture media; embryogenesis; explants; forage; genetic transformation; genotype; glutamine; grains; magnesium sulfate; meristems; models; morphogenesis; new species; potassium phosphates; proline; recalcitrant species; shoot tips; tropical grasses
Abstract:
... Slow callus growth is a barrier to efficient genetic transformation in some gramineous species. A reformulation of Murashige and Skoog (MS) medium, with additional magnesium sulphate, potassium phosphate, copper sulphate, proline and glutamine, termed WPBS medium, has been developed which improves all aspects of in vitro culture when compared with MS based media. Embryogenic callus could be produc ...
DOI:
10.1007/s11240-020-01784-8
PubMed:
32308245
PubMed Central:
PMC7145791

18. Micropropagation of Bambusa oldhamii Munro in heterotrophic, mixotrophic and photomixotrophic systems

Author:
Silveira, Andreia Alves da Costa; Lopes, Francis Julio Fagundes; Sibov, Sérgio Tadeu
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 315-326
ISSN:
0167-6857
Subject:
Bambusa oldhamii; bamboos; culture flasks; dry season; explants; fluorescent lighting; gas exchange; leaves; micropropagation; paclobutrazol; plant tissues; shoots; sprouting; thidiazuron
Abstract:
... Photomixotrophic micropropagation systems have been associated with improved development of several plant species. In this work, we established an efficient protocol for the in vitro micropropagation of Bambusa oldhamii Munro by studying the influence of the rainy and dry seasons on plant tissue contamination, the effects of the gaseous atmosphere inside the culture flasks, and the effects of radi ...
DOI:
10.1007/s11240-020-01788-4

19. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis

Author:
Ćuković, Katarina; Dragićević, Milan; Bogdanović, Milica; Paunović, Danijela; Giurato, Giorgio; Filipović, Biljana; Subotić, Angelina; Todorović, Slađana; Simonović, Ana
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 417-433
ISSN:
0167-6857
Subject:
Centaurium erythraea; adenosine kinase; adventitious buds; binding proteins; biosynthesis; callus; databases; essential genes; explants; in vitro regeneration; leaves; medicinal plants; morphogenesis; rats; ribosomal proteins; roots; sarcoma; secondary metabolites; somatic embryogenesis; somatic embryos; transcriptome
Abstract:
... Centaurium erythraea Rafn. (common centaury, Gentianaceae) is a medicinal plant with great regeneration potential and developmental plasticity in vitro. Centaury can be regenerated from leaf explants by both somatic embryogenesis (SE) and shoot development (SD). We believe that its regeneration potential and developmental plasticity rest on high activity of certain genes, which may not be active o ...
DOI:
10.1007/s11240-020-01801-w

20. Polyphenol-induced recalcitrance alleviated in katsuratree (Cercidiphyllum spp.) microshoot cultures

Author:
Carlson, Craig H.; West, Todd P.
Source:
Plant cell, tissue, and organ culture 2020 v.141 no.2 pp. 371-379
ISSN:
0167-6857
Subject:
Cercidiphyllum japonicum; aqueous solutions; ascorbic acid; carbon; chelating agents; explants; growth traits; micropropagation; polyphenols; presoaking; sucrose
Abstract:
... KEY MESSAGE: Approach for reducing the growth-inhibiting effects of endogenous phenolic compounds in katsuratree (Cercidiphyllum japonicum). An approach for reducing the growth-inhibiting effects of endogenous phenolic compounds in katsuratree (Cercidiphyllum japonicum Sieb. & Zucc.) was developed to assist with clonal micropropagation of the species. A quantitative phenolic profile of Cercidiphyl ...
DOI:
10.1007/s11240-020-01793-7