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You searched for: Journal name Plant cell, tissue, and organ culture Remove constraint Journal name: Plant cell, tissue, and organ culture Publication year rev 7995-2005 Remove constraint Publication year rev: 7995-2005 Source 2005 v.80 no.2 Remove constraint Source: 2005 v.80 no.2
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1. Aluminum borate whisker-mediated production of transgenic tobacco plants

Author:
Mizuno, K.; Takahashi, W.; Beppu, T.; Shimada, T.; Tanaka, O.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 163-169
ISSN:
0167-6857
Subject:
Nicotiana tabacum; tobacco; leaves; genetic transformation; callus; gene transfer; aluminum; borate minerals; silicon; carbides; agitation; transgenic plants; histochemistry; beta-glucuronidase; enzyme activity
Abstract:
... An aluminum borate whiskers-mediated transformation system for calluses of tobacco (Nicotiana tabacum, cv. SR-1) has been developed. A total of 50 small pieces of calluses were vigorously agitated in a liquid medium containing aluminum borate whiskers, pBI221 plasmid carrying the beta-glucuronidase (GUS) gene, and pBI222 plasmid carrying the hygromycin phosphotransferase (HPT) gene. After treatmen ...

2. Calluses initiated from thin mature embryo fragments are suitable targets for wheat transformation as assessed by long-term GUS expression studies

Author:
Delporte, F.; Li, S.; Jacquemin, J.M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 139-149
ISSN:
0167-6857
Subject:
genetic transformation; dose response; biolistics; transgenic plants; embryo (plant); Agrobacterium radiobacter; explants; wheat; gene expression; Triticum aestivum; 2,4-D; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Microprojectile- or Agrobacterium-mediated DNA delivery into calluses initiated from immature embryos has proven to be effective in transforming wheat. Yet, obtaining a large number of high quality immature embryos throughout the year is a laborious and delicate process. To circumvent these limitations, we propose an alternative technique applying the particle bombardment technology to calluses de ...

3. Histology of embryogenic responses in soybean anther culture

Author:
Rodrigues, L.R.; Oliveira, J.M.S.; Mariath, J.E.A.; Bodanese-Zanettini, M.H.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 129-137
ISSN:
0167-6857
Subject:
anther culture; Glycine max; anthers; histology; developmental stages; soybeans; somatic embryogenesis; benzyladenine; 2,4-D; micropropagation; in vitro regeneration; culture media; somatic embryos
Abstract:
... In order to clarify the embryogenic responses in soybean anther culture, anthers of four cultivars were cultured under known conditions to trigger androgenic response. A histological study was performed with anthers in vivo and with approximately 100 explants sampled after 9, 12, 15, 18, 21, 30 and 45 days of culture. In vitro culture triggered the frequent accumulation of phenolic compounds on th ...

4. Induction of streptomycin-resistant plantlets in Solanum surattense through in vitro mutagenesis

Author:
Rama Swamy, N.; Ugandhar, T.; Praveen, M.; Rambabu, M.; Upender, M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 201-207
ISSN:
0167-6857
Subject:
chloroplast DNA; medicinal plants; streptomycin; benzyladenine; micropropagation; dose response; explants; in vitro regeneration; Solanum virginianum; mutagenesis; culture media; indole acetic acid; plant breeding; antibiotic resistance; adventitious shoots; cotyledons; mutants; gamma radiation; selection criteria
Abstract:
... The species Solanum surattense Burm.f. has importance in ayurvedic medicine and also as vegetable. Streptomycin-resistant plantlets were induced showing chloroplast encoded mutants in S. surattense from mutagenised (ethyl methane sulphonate and gamma-rays) cotyledon explants. Chloroplast encoded - streptomycin resistant - shoots were developed from green (unbleached) sectors of the cotyledons. The ...

5. Plant regeneration of three Opuntia genotypes used as human food

Author:
Garcia-Saucedo, P.A.; Valdez-Morales, M.; Valverde, M.E.; Cruz-Hernandez, A.; Paredes-Lopez, O.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 215-219
ISSN:
0167-6857
Subject:
buds; dose response; Opuntia ficus-indica; gibberellic acid; explants; cacti and succulents; indole butyric acid; benzyladenine; genotype; micropropagation; genetic variation; in vitro regeneration; culture media; shoots; Mexico
Abstract:
... In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 micromolar BA was the best for bud formation. Satisfactory rooting occu ...

6. Regeneration from leaf-base explants of Lolium perenne L. and Lolium multiflorum L

Author:
Newell, C.A.; Gray, J.C.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 233-237
ISSN:
0167-6857
Subject:
lawns and turf; genes; benzyladenine; micropropagation; 2,4-D; callus; dose response; explants; gene expression; embryogenesis; in vitro regeneration; leaves; Lolium perenne; enzyme activity; culture media; Lolium multiflorum; genetic transformation; forage crops; biolistics; transgenic plants; beta-glucuronidase
Abstract:
... The regenerability of Lolium perenne and Lolium multiflorum was investigated using leaf-base sections excised from plantlets grown in vitro. Young leaf bases were cut into three sections each 2 mm in length, the lowest 0-2 mm section adjacent to, but not including, the apical meristem. Callus development followed by embryogenesis occurred only in the lowest 2 mm leaf-base sections of L. perenne cv ...

7. Sucrose in storage media and cultivar affects post-storage regrowth of in vitro Hosta propagules

Author:
Gollagunta, V.; Adelberg, J.W.; Rieck, J.; Rajapakse, N.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 191-199
ISSN:
0167-6857
Subject:
ornamental plants; cultivars; dose response; cell growth; Hosta; sucrose; propagation materials; regrowth; micropropagation; genetic variation; temperature; in vitro regeneration; culture media; tissue culture
Abstract:
... The goal of this research was to investigate if culturing in high sucrose (5%) liquid media during multiplication phase (stage II) would enhance endogenous sugar levels and dry matter sufficiently to allow storage of in vitro plants in sugar free media without adversely affecting post-storage recovery. Hosta tokudama 'Newberry Gold (NBG)' and Hosta 'Striptease' were cultured in Murashige and Skoog ...

8. A protocol for in vitro germination and sustainable growth of two tropical mistletoes

Author:
Ang, S.L.P.; Youn, J.W.H.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 221-228
ISSN:
0167-6857
Subject:
germination; in vitro culture; Dendrophthoe; cell growth; dose response; medicinal plants; coconut milk; benzyladenine; micropropagation; in vitro regeneration; culture media; parasitic plants; callus; shoots; Singapore
Abstract:
... We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis. Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15-20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the ...

9. Callus induction and de novo regeneration from callus in Guar (Cyamopsis tetragonoloba)

Author:
Prem, D.; Singh, S.; Gupta, P.P.; Singh, J.; Kadyan, S.P.S.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 209-214
ISSN:
0167-6857
Subject:
kinetin; dose response; indole butyric acid; internodes; guar gum; Cyamopsis tetragonoloba; cotyledons; explants; benzyladenine; 2,4-D; indole acetic acid; naphthaleneacetic acid; micropropagation; in vitro regeneration; culture media; callus; shoots
Abstract:
... Guar (Cyamopsis tetragonoloba L. Taub) is a drought tolerant and multipurpose grain legume cash crop grown primarily under rainfed conditions in several countries. The effect of various growth regulators and their combinations on a variety of explants, namely the embryo, cotyledons, cotyledonary nodes, shoot tip and hypocotyle, has been studied and an efficient system for callus induction and rege ...

10. Development of high frequency multiple shoot formation in Persian clover (Trifolium resupinatum L.)

Author:
Uranbey, S.; Sevimay, C.S.; Ozcan, S.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 229-232
ISSN:
0167-6857
Subject:
Trifolium resupinatum; kinetin; hypocotyls; indole butyric acid; internodes; explants; cotyledons; forage legumes; rooting; benzyladenine; naphthaleneacetic acid; micropropagation; in vitro regeneration; culture media; indole acetic acid; shoots; Turkey (country)
Abstract:
... An efficient and reliable micropropagation system for Persian clover (Trifolium resupinatum L.) was developed using different explants and media. Node, hypocotyl and cotyledonary node explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of either 6-benzyladenine (BA) and indole-3-butyric acid (IBA) or BA, Kinetin (KIN) and IBA. Direct multiple shoots developed w ...

11. Effect of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos

Author:
Garcia-Martin, G.; Manzanera, J.A.; Gonzalez-Benito, M.E.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 171-177
ISSN:
0167-6857
Subject:
Quercus suber; forest trees; in vitro culture; somatic embryos; plant development; in vitro regeneration; culture media; abscisic acid; indole acetic acid; quantitative analysis; plant growth; Spain
Abstract:
... Knowledge of the relationship between indole-3-acetic acid (IAA) and abscisic acid (ABA) is relevant to control the development and the maturation of cork oak (Quercus suber L.) somatic embryos. The addition of 1 micromolar ABA to the culture medium significantly promoted somatic embryo maturation and increased both fresh and dry matter without affecting the relative water content. This effect was ...

12. Micrografting of ASBVd-infected avocado (Persea americana) plants

Author:
Suarez, I.E.; Schnell, R.A.; Kuhn, D.N.; Litz, R.E.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 179-185
ISSN:
0167-6857
Subject:
cultivars; in vitro culture; Persea americana; disease control; avocados; Avsunviroidae; disease resistance; micropropagation; genetic variation; rootstock-scion relationships
Abstract:
... Shoot tips (meristem plus 2-3 leaf primordia) from in vitro-germinated avocado seedlings of 2 ASBVd-infected cultivars were micrografted in vitro onto decapitated seedlings from 2 ASBVd-free cultivars, and plants were recovered. Shoot tips consisted of two different sizes, i.e., <0.5 mm long and >0.5 mm but <1 mm long. The recovered plants were indexed for ASBVd using RT-PCR. More plants (58.8%) w ...
Handle:
10113/30550

13. Micropropagation of Hagenia abyssinica: a multipurpose tree

Author:
Feyissa, T.; Welander, M.; Negash, L.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 119-127
ISSN:
0167-6857
Subject:
Rosaceae; tropics; developmental stages; explants; gibberellic acid; indole butyric acid; rooting; benzyladenine; 2,4-D; indigenous species; micropropagation; in vitro regeneration; culture media; shoots; Africa
Abstract:
... An in vitro propagation protocol has been developed for Hagenia abyssinica using original material from both juvenile and mature trees. Juvenile explants were obtained from seedlings, as well as shoots and meristems from 5 to 7-month-old greenhouse grown plants. Shoots collected from stem bases of five genotypes were used to establish cultures from mature trees. Explants of seedling origin were us ...

14. Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis

Author:
Yuan, S.; Yan, Y.C.; Lin, H.H.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 157-161
ISSN:
0167-6857
Subject:
somatic embryos; stems; indole butyric acid; explants; somatic embryogenesis; 2,4-D; benzyladenine; naphthaleneacetic acid; micropropagation; Dioscorea; in vitro regeneration; culture media; callus; yams
Abstract:
... A new method was established for somatic embryogenesis and plant regeneration from callus cultures of Dioscorea zingiberensis C.H. Wright. Primary callus was induced by culturing stems, leaves and petioles on Murashige and Skoog (MS) medium supplemented with 0.5-2.0 mg l(-1) N6-benzyladenine (BA) and 0-2.0 mg l(-1) alpha-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) for 1 ...

15. Rosmarinic acid production by Coleus forskohlii hairy root cultures

Author:
Li, W.; Koike, K.; Asada, Y.; Yoshikawa, T.; Nikaido, T.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 151-155
ISSN:
0167-6857
Subject:
Plectranthus barbatus; medicinal plants; roots; in vitro culture; culture media; sucrose; methyl jasmonate; salicylic acid; yeast extract; dose response; rosmarinic acid; biosynthesis; Rhizobium rhizogenes; plant pathogenic bacteria; cultured cells
Abstract:
... Coleus forskohlii hairy root cultures were found to produce forskolin and rosmarinic acid (RA) as the main metabolites. The growth and RA production by C. forskohlii hairy root cultures in various liquid media were examined. The hairy root cultures showed good growth in hormone-free Murashige and Skoog medium containing 3% (w/v) sucrose (MS medium), and Gamborg B5 medium containing 2% (w/v) sucros ...

16. Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron

Author:
Faisal, M.; Ahmad, N.; Anis, M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 187-190
ISSN:
0167-6857
Subject:
endangered species; dose response; indole butyric acid; internodes; medicinal plants; Rauvolfia; explants; benzyladenine; naphthaleneacetic acid; micropropagation; germplasm conservation; in vitro regeneration; culture media; indole acetic acid; shoots; India
Abstract:
... The effect of thidiazuron (TDZ) was investigated on in vitro shoot proliferation from nodal explants of Rauvolfia tetraphylla. Murashige and Skoog (MS) medium containing TDZ (0.5-10 micromolar) was effective in inducing shoot buds and maintaining high rates of shoot multiplication on hormone free medium. The highest shoot regeneration frequency (90%) and mean number (18.50 +/- 1.25) of shoots per ...