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- Mollel, M.H.N.; Goyvaerts, E.M.A.
- Plant cell, tissue, and organ culture 2004 v.79 no.3 pp. 321-328
- fruit trees, etc ; indigenous species; Sclerocarya birrea; genetic improvement; genetic transformation; Agrobacterium tumefaciens; gene transfer; transgenic plants; gene expression; shoots; in vitro culture; in vitro regeneration; culture media; benzyladenine; gibberellins; kinetin; dose response; micropropagation; methodology; Africa; Show all 20 Subjects
- ... In planta Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birreasubsp. caffra(Anacardiaceae) resulted in chimeric transgenic in vitro shoots at a rate of 0.8--1.5%. Average transgene expression rates of 33.1 and 1.3% GUS-positive explants were observed on days 3 and 6 after agroinfection, respectively. One to 4 GUS-positive zones were observed per GUS-positive explant sect ...
- Nand, N.; Drew, R.A.; Ashmore, S.
- Plant cell, tissue, and organ culture 2004 v.77 no.2 pp. 193-201
- fruit trees, etc ; indigenous species; Cunoniaceae; micropropagation; tissue culture; explants; culture media; cytokinins; benzyladenine; isopentenyladenine; kinetin; plant growth substances; chemical concentration; plant growth; shoots; root growth; genotype; mature plants; juvenility; Australia; Show all 20 Subjects
- ... Nodal explants from in vitro grown seedlings of Davidsonia pruriens and D. jerseyana, established on MS media were treated with various concentrations of three cytokinins. D. pruriens developed optimum shoot growth in terms of shoot height and number of leaves per shoot when 1.0 micromolar BA was added to basal MS medium while optimum shoot growth for D. jerseyana was obtained when 0.01 micromolar ...