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- Di Cola, A.; Costantino, P.; Spano, L.
- Plant cell, tissue, and organ culture 1996 v.46 no.3 pp. 203-209
- developmental stages; Rhizobium rhizogenes; cell differentiation; roots; stems; explants; gene expression; Nicotiana tabacum; naphthaleneacetic acid; leaves; morphogenesis; transcription (genetics); enzyme activity; culture media; indole acetic acid; rhizogenesis; genetic transformation; gene transfer; transgenic plants; reporter genes; beta-glucuronidase
- ... The relative role played in the induction of root differentiation by the morphogenetic gene rolB, present in the TDNA of Agrobacterium rhizogenes, and the specific commitment of different plant cell types, has been evaluated by means of an in vitro-rooting assay. Explants of different origin derived from transgenic tobacco plants transformed by both the rolB gene and the GUS reporter gene (E. coli ...
- Castano, C.I.; Profit, M.P. de.
- Plant cell, tissue, and organ culture 1996 v.46 no.3 pp. 211-218
- viability; cultivars; dose response; seed set; explants; flowers; culture media; fertilization (reproduction); light; photoperiod; genetic variation; embryogenesis; pollination; roots; Cichorium intybus
- ... Formation of viable seeds of Cichorium intybus L. was achieved in an in vitro system. Flower formation, pollination, fertilization, embryogenesis and seed development occurred in vitro on chicory root explants on culture medium lacking plant growth regulators. After flower induction under a 24-h daylength treatment, the explants were transferred to a 16-h daylength at 40 microeinsteins m-2 s-1 irr ...