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You searched for: Journal Plant cell reports Remove constraint Journal: Plant cell reports Publication Year 2008 Remove constraint Publication Year: 2008 Source 2008 v.27 no.6 Remove constraint Source: 2008 v.27 no.6
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1. Agrobacterium tumefaciens-mediated transformation of poinsettia, Euphorbia pulcherrima, with virus-derived hairpin RNA constructs confers resistance to Poinsettia mosaic virus

Author:
Clarke, Jihong Liu; Spetz, Carl; Haugslien, Sissel; Xing, Shaochen; Dees, Merete W.; Moe, Roar; Blystad, Dag-Ragnar
Source:
Plant cell reports 2008 v.27 no.6 pp. 1027-1038
ISSN:
0721-7714
Subject:
Agrobacterium radiobacter; Euphorbia pulcherrima; Northern blotting; Poinsettia mosaic virus; RNA; Southern blotting; color; disease resistance; enzyme-linked immunosorbent assay; genes; molecular weight; nuclear genome; ornamental plants; polymerase chain reaction; sexual reproduction; somatic embryogenesis; somatic embryos; transgenic plants
Abstract:
... Agrobacterium-mediated transformation for poinsettia (Euphorbia pulcherrima Willd. Ex Klotzsch) is reported here for the first time. Internode stem explants of poinsettia cv. Millenium were transformed by Agrobacterium tumefaciens, strain LBA 4404, harbouring virus-derived hairpin (hp) RNA gene constructs to induce RNA silencing-mediated resistance to Poinsettia mosaic virus (PnMV). Prior to trans ...
DOI:
10.1007/s00299-008-0526-9
PubMed:
18327592
PubMed Central:
PMC2413081

2. Arabidopsis R2R3-MYB transcription factor AtMYB60 functions as a transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca sativa)

Author:
Park, Jong-Sug; Kim, Jung-Bong; Cho, Kang-Jin; Cheon, Choong-Ill; Sung, Mi-Kyung; Choung, Myoung-Gun; Roh, Kyung-Hee
Source:
Plant cell reports 2008 v.27 no.6 pp. 985-994
ISSN:
0721-7714
Subject:
engineering; transcription factors; Arabidopsis; biosynthesis; leaf lettuce; cyanidin; new variety; phenotype; pigmentation; Lactuca sativa; delphinidin; secondary metabolites; genes; irradiation; ultraviolet radiation
Abstract:
... The MYB transcription factors play important roles in the regulation of many secondary metabolites at the transcriptional level. We evaluated the possible roles of the Arabidopsis R2R3-MYB transcription factors in flavonoid biosynthesis because they are induced by UV-B irradiation but their associated phenotypes are largely unexplored. We isolated their genes by RACE-PCR, and performed transgenic ...
DOI:
10.1007/s00299-008-0521-1
PubMed:
18317777
PubMed Central:
PMC2413084

3. AtCopeg1, the unique gene originated from AtCopia95 retrotransposon family, is sensitive to external hormones and abiotic stresses

Author:
Duan, Ke; Ding, Xiangzhen; Zhang, Qiong; Zhu, Hong; Pan, Aihu; Huang, Jianhua
Source:
Plant cell reports 2008 v.27 no.6 pp. 1065-1073
ISSN:
0721-7714
Subject:
Arabidopsis; abiotic stress; consensus sequence; evolution; genes; hormones; intergenic DNA; promoter regions; retrotransposons; terminal repeat sequences; transposons
Abstract:
... Retrotransposons, the important component of eukaryotic genome, are seeds of evolution and play great role in creating new genes. The compact Arabidopsis genome harbors over 200 Copia-like retrotransposons, but mostly silent. Here we isolated an expressed gene AtCopeg1 (Copia evolved gene 1), which shows higher than 90% identity to AtCopia95_I, the consensus sequence encoding AtCopia95 polyprotein ...
DOI:
10.1007/s00299-008-0520-2
PubMed:
18309491

4. Competitive interaction between two functional S-haplotypes confer self-compatibility on tetraploid Chinese cherry (Prunus pseudocerasus Lindl. CV. Nanjing Chuisi)

Author:
Huang, S.-X.; Wu, H.-Q.; Li, Y.-R.; Wu, J.; Zhang, S.-J.; Heng, W.; Zhang, S.-L.
Source:
Plant cell reports 2008 v.27 no.6 pp. 1075-1085
ISSN:
0721-7714
Subject:
Prunus pseudocerasus; Scrophulariaceae; Solanaceae; cherries; cultivars; diploidy; gametophytes; genes; mutation; pollen; polymerase chain reaction; self-pollination; tetraploidy
Abstract:
... Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. pseudocerasus S-RNase) and PpsSFB (P. pseudocerasus S-haplotype-specific F-box) hav ...
DOI:
10.1007/s00299-008-0528-7
PubMed:
18327590

5. Construction of the physical map of the gpa7 locus reveals that a large segment was deleted during rice domestication

Author:
Li, Xianran; Tian, Feng; Huang, Haiyan; Tan, Lubin; Zhu, Zuofeng; Hu, Songnian; Sun, Chuanqing
Source:
Plant cell reports 2008 v.27 no.6 pp. 1087-1092
ISSN:
0721-7714
Subject:
Oryza rufipogon; bacterial artificial chromosomes; clones; domestication; inflorescences; loci; molecular cloning; quantitative trait loci; wild rice; China
Abstract:
... To facilitate cloning gene(s) underlying gpa7, a deep-coverage BAC library was constructed for an isolate of common wild rice (Oryza rufipogon Griff.) collected from Dongxiang, Jiangxi Province, China (DXCWR). gpa7, a quantitative trait locus corresponding to grain number per panicle, is positioned in the short arm of chromosome 7. The BAC library containing 96,768 clones represents approximate 18 ...
DOI:
10.1007/s00299-008-0529-6
PubMed:
18317774

6. Creation and analysis of a novel chimeric promoter for the complete containment of pollen- and seed-mediated gene flow

Author:
Liu, Zongrang; Zhou, Changhe; Wu, Keqiang
Source:
Plant cell reports 2008 v.27 no.6 pp. 995
ISSN:
0721-7714
Subject:
pollen; seeds; gene flow; promoter regions; transgenic plants; carpels; gene expression; gynoecium; chimerism; Arabidopsis thaliana
Abstract:
... Effective containment of gene flow in transgenic plants requires a promoter that is highly specific for male and female gametes or tissues. Here, we report the creation of a novel pollen-, stigma- and carpel-specific (PSC) promoter through the fusion of the pollen-specific LAT52 and carpel-specific AGL5 enhancers to a stigma-specific SLG promoter. Gene expression analysis showed that fusion of the ...
Handle:
10113/23600
DOI:
10.1007/s00299-008-0522-0
PubMed:
18317776

7. Development of a transient expression system in grapevine via agro-infiltration

Author:
Santos-Rosa, M.; Poutaraud, A.; Merdinoglu, D.; Mestre, P.
Source:
Plant cell reports 2008 v.27 no.6 pp. 1053-1063
ISSN:
0721-7714
Subject:
Agrobacterium; Plasmopara viticola; disease resistance; downy mildew; gene expression; genes; genotype; green fluorescent protein; leaves; pathogens; stilbenes; virulence
Abstract:
... Transient expression of genes using Agrobacterium is a powerful tool for the analysis of gene function in plants. We have developed this method for the analysis of genes involved in disease resistance in grapevine leaves. Our research showed that the quality of the plant material, the plant genotype used for agro-infiltration and the presence of additional virulence factors (carried on plasmid pCH ...
DOI:
10.1007/s00299-008-0531-z
PubMed:
18317773

8. Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum

Author:
LaO, María; Arencibia, Ariel D.; Carmona, Elva R.; Acevedo, Ricardo; Rodríguez, Eida; León, Ondina; Santana, Ignacio
Source:
Plant cell reports 2008 v.27 no.6 pp. 1103-1111
ISSN:
0721-7714
Subject:
Saccharum; Sporisorium; auxins; cultivars; ethylene; gene expression regulation; genotype; hosts; major genes; pathogens; plant response; sugarcane
Abstract:
... A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67.2%) of the differential TDFs up-regulated in the resistant M31/45 cultivar. The p ...
DOI:
10.1007/s00299-008-0524-y
PubMed:
18379790

9. Elevated H₂O₂ production via overexpression of a chloroplastic Cu/ZnSOD gene of lily (Lilium oriental hybrid 'Marco Polo') triggers ethylene synthesis in transgenic potato

Author:
Kim, Yoon-Sik; Kim, Hyun-Soon; Lee, Yong-Hwa; Kim, Mi-Sun; Oh, Hyun-Woo; Hahn, Kyu-Woong; Joung, Hyouk; Jeon, Jae-Heung
Source:
Plant cell reports 2008 v.27 no.6 pp. 973-983
ISSN:
0721-7714
Subject:
1-aminocyclopropane-1-carboxylate synthase; 1-aminocyclopropane-1-carboxylic acid; Lilium; Northern blotting; ethylene; ethylene inhibitors; ethylene production; gene expression; genes; hybrids; potatoes; root hairs; stem elongation; superoxide dismutase; transgenic plants
Abstract:
... Transgenic potato plants (SS2 and SS4) that overexpressed a chloroplastic copper/zinc superoxide dismutase lily gene were utilized as an H₂O₂-inducible system in order to study the role of H₂O₂ as a signaling molecule in the biosynthesis of ethylene. SS2 and SS4 plants grown in vitro under sealed microenvironment (SME) conditions displayed anomalous phenotypes including reduction of stem elongatio ...
DOI:
10.1007/s00299-008-0515-z

10. Evaluation of selection strategies alternative to nptII in genetic transformation of citrus

Author:
Ballester, Alida; Cervera, Magdalena; Peña, Leandro
Source:
Plant cell reports 2008 v.27 no.6 pp. 1005-1015
ISSN:
0721-7714
Subject:
Citroncirus webberi; Citrus; antibiotic resistance; excision; genetic markers; genetic transformation; genotype; in vitro culture; kanamycin kinase; oranges; reporter genes; screening; shoots; transgenic plants
Abstract:
... The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. The present work investigates three different selection alternatives, comparing them to nptII selection in two citrus genotypes, Carrizo citrange ...
DOI:
10.1007/s00299-008-0523-z
PubMed:
18317775

11. Expression of a synthetic cry1EC gene for resistance against Spodoptera litura in transgenic peanut (Arachis hypogaea L.)

Author:
Tiwari, Siddharth; Mishra, Devesh K.; Singh, Ankit; Singh, P. K.; Tuli, Rakesh
Source:
Plant cell reports 2008 v.27 no.6 pp. 1017-1025
ISSN:
0721-7714
Subject:
Southern blotting; insect pests; peanuts; Spodoptera litura; transgenic plants; Agrobacterium radiobacter; polymerase chain reaction; explants; cotyledons; Western blotting; bioassays; enzyme-linked immunosorbent assay; gene expression; synthetic genes; larvae; Arachis hypogaea; leaves; plant proteins
Abstract:
... The tobacco cutworm (Spodoptera litura) is a polyphagous foliage insect and a major pest on peanut (Arachis hypogaea L.). S. litura is susceptible to the chimeric δ-endotoxin Cry1EC reported earlier. De-embryonated cotyledon explants of peanut were transformed using Agrobacterium tumefaciens strain EHA101 harboring a synthetic cry1EC gene driven by the CaMV 35S promoter. Transgenic plants of peanu ...
DOI:
10.1007/s00299-008-0525-x
PubMed:
18320194

12. Genetic control of somatic embryogenesis induction in Eucalyptus globulus Labill

Author:
Pinto, G.; Park, Y. -S.; Neves, L.; Araújo, C.; Santos, C.
Source:
Plant cell reports 2008 v.27 no.6 pp. 1093-1101
ISSN:
0721-7714
Subject:
Eucalyptus globulus; diallel analysis; general combining ability; maternal effect; somatic embryogenesis; somatic embryos; specific combining ability; tree breeding; trees
Abstract:
... A reproducible protocol for somatic embryogenesis (SE) induction in Eucalyptus globulus from mature zygotic embryos is available since 2002. However, for the use of SE in tree breeding programs, the frequency of SE initiation needs to be improved and controlled, and this was investigated in 13 open-pollinated (OP) families over three consecutive years. A diallel mating design with five parent tree ...
DOI:
10.1007/s00299-008-0532-y
PubMed:
18317772

13. Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regeneration from banana (Musa AAA, cv. 'Dwarf Cavendish') male flowers

Author:
Pérez-Hernández, Juan Bernardo; Rosell-García, Purificación
Source:
Plant cell reports 2008 v.27 no.6 pp. 965-971
ISSN:
0721-7714
Subject:
Musa; bananas; buds; cell suspension culture; male flowers; mature plants; plant growth substances; somatic embryogenesis
Abstract:
... Availability of explants with adequate embryogenic competence is one of the most important limitations for the development of regenerable cell suspensions in banana. To increase the number and ease of accessibility to potentially embryogenic explants, a novel methodology is described by which young male flower clusters isolated from adult plants are induced to form new flower buds and proliferate ...
DOI:
10.1007/s00299-008-0509-x

14. Morphometric and biochemical characterization of red beet (Beta vulgaris L.) hairy roots obtained after single and double transformations

Author:
Thimmaraju, R.; Venkatachalam, L.; Bhagyalakshmi, N.
Source:
Plant cell reports 2008 v.27 no.6 pp. 1039-1052
ISSN:
0721-7714
Subject:
Beta vulgaris; Rhizobium rhizogenes; auxins; beets; biomass; cells; clones; functional properties; genes; morphometry; peroxidase; physiological state; pigments; plant development; plant hormones; roots; secondary metabolites; transfer DNA
Abstract:
... It is known that T-DNA of Agrobacterium rhizogenes affects processes of plant development and activates the synthesis of secondary metabolites in transformed plant cells. In the present investigation, we provide evidence that different strains of A. rhizogenes significantly affect morphometric, morphological and functional characteristics of hairy roots of red beet (Beta vulgaris L.). Infection wi ...
DOI:
10.1007/s00299-008-0527-8

15. Plastid-expressed choline monooxygenase gene improves salt and drought tolerance through accumulation of glycine betaine in tobacco

Author:
Zhang, Jiang; Tan, Wei; Yang, Xing-Hong; Zhang, Hong-Xia
Source:
Plant cell reports 2008 v.27 no.6 pp. 1113-1124
ISSN:
0721-7714
Subject:
Beta vulgaris; Nicotiana tabacum; ammonium compounds; beets; betaine; biosynthesis; choline; choline monooxygenase; drought tolerance; genetic engineering; leaves; operon; osmotolerance; photochemistry; photosystem II; ribosomal RNA; roots; salt stress; seeds; sodium chloride; stress tolerance; tobacco; toxicity; transgenic plants; water stress
Abstract:
... Glycine betaine (GlyBet), a quaternary ammonium compound, functions as an osmoprotectant in many organisms including plants. Previous research has shown that over-expression of enzymes for GlyBet biosynthesis in transgenic plants improved abiotic stress tolerance, but so far no study on the effects of plastid-expression of choline monooxygenase, the enzyme that catalyzes the conversion of choline ...
DOI:
10.1007/s00299-008-0549-2
PubMed:
18437388