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You searched for: Journal Plant cell reports Remove constraint Journal: Plant cell reports Publication Year 1997 Remove constraint Publication Year: 1997 Source 1997 v.16 no.5 Remove constraint Source: 1997 v.16 no.5
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1. Agrobacterium strain specificity and shooty tumour formation in eucalypt (Eucalyptus grandis X E. urophylla)

Author:
Machado, L. de O.R.; Andrade, G.M. de.; Barrueto Cid, L.P.; Penchel, R.M.; Brasileiro, A.C.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 299-303
ISSN:
0721-7714
Subject:
Eucalyptus grandis; Eucalyptus urophylla; genetic transformation; Agrobacterium tumefaciens; Rhizobium rhizogenes; gene transfer; methodology; pathogenicity; strain differences; neoplasms
Abstract:
... To develop a successful protocol for Agrobacterium-mediated transformation in plants it is essential to determine the most efficient bacterial strain/plant genotype interaction. In the present work, we evaluated the susceptibility of Eucalyptus grandis x E. urophylla to five Agrobacterium rhizogenes and twelve A. tumefaciens wild-type strains. The results showed different degrees of virulence, acc ...
DOI:
10.1007/BF01088285

2. An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)

Author:
Cabrera-Ponce, J.L.; Lopez, L.; Assad-Garcia, N.; Medina-Arevalo, C.; Bailey, A.M.; Herrera-Estrella, L.
Source:
Plant cell reports 1997 v.16 no.5 pp. 255-260
ISSN:
0721-7714
Subject:
Asparagus officinalis; herbicide resistance; plant breeding; embryo (plant); callus; genetic transformation; DNA; gene transfer; methodology; transgenic plants; cultivars; genetic variation; plasmids; genetic code; phosphotransferases (phosphomutases); glufosinate; acyltransferases; beta-glucuronidase; culture media; gene expression; enzyme activity; histochemistry
Abstract:
... The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl tran ...
DOI:
10.1007/s002990050218

3. Chromosome doubling via tuber disc culture in dihaploid potato as determined by confocal microscopy

Author:
Mozafari, J.; Wolyn, D.J.; Ali-Khan, S.T.
Source:
Plant cell reports 1997 v.16 no.5 pp. 329-333
ISSN:
0721-7714
Subject:
Solanum tuberosum; tubers; in vitro culture; chromosome addition; tetraploidy; somaclonal variation; leaves; guard cells; chloroplasts; chromosome number; rapid methods; developmental stages; plant anatomy; haploidy; genotype
Abstract:
... The potential of tuber disc culture for chromosome doubling was investigated in somaclonal populations of four dihaploid genotypes and one tetraploid cultivar of potato (Solanum tuberosum). Laser scanning confocal microscopy (LSCM) was used for rapid determination of the ploidy level based on the number of chloroplasts in stomatal guard cells of leaves. Factorial analysis of chloroplast number in ...
DOI:
10.1007/BF01088291

4. Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspension cultures

Author:
Godoy-Hernandez, G.; Loyola-Vargas, V.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 287-290
ISSN:
0721-7714
Subject:
Catharanthus roseus; neoplasms; cell lines; cell suspension culture; metabolism; aspirin; dose response; Aspergillus fumigatus; cinnamic acid; secondary metabolites; biosynthesis; alkaloids; phenolic compounds; furans; coumarins; quantitative analysis; anthocyanins; culture media; carbon; corn starch
Abstract:
... Addition of various concentrations (0.5-20 mM) of acetylsalicylic acid (ASA) to tumor lines of Catharanthus roseus cultivated in vitro and requiring corn starch as carbon source, produced remarkable effects on secondary metabolite production. An increase of 505% total alkaloids per culture (cells plus liquid medium), 1587% total phenolics (liquid medium), 612% total furanocoumarins (liquid medium) ...
DOI:
10.1007/BF01088282

5. Effect of atmospheric pressure on sunflower (Helianthus annuus L.) protoplast division

Author:
Barthou, H.; Briere, C.; Caumont, C.; Petitprez, M.; Kallerhoff, J.; Borin, C.; Souvre, A.; Alibert, G.
Source:
Plant cell reports 1997 v.16 no.5 pp. 310-314
ISSN:
0721-7714
Subject:
Helianthus annuus; hypocotyls; protoplasts; in vitro culture; culture media; cell division; atmospheric pressure; turgor; cell walls; biosynthesis; cytoskeleton; developmental stages; plant anatomy
Abstract:
... Sunflower protoplasts were cultured in liquid medium under high atmospheric pressure (0.2 to 0.6 MPa) and the plating efficiency, cell wall synthesis and microtubule organization were assessed. In 7-day-old cultures under a pressure of 0.4 MPa and above, the division rate was strongly reduced by more than 60% as compared to the control. Although most of the protoplasts had begun to regenerate a ne ...
DOI:
10.1007/s002990050229

6. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

7. Enhanced production of scopolin by Solanum aviculare cells immobilised within Ca-alginate gel beads

Author:
Roisin, C.; Gillet-Manceau, F.; Nava Saucedo, J.E.; Fliniaux, M.; Jacquin-Dubreuil, A.; Barbotin, J.N.
Source:
Plant cell reports 1997 v.16 no.5 pp. 349-353
ISSN:
0721-7714
Subject:
Solanum aviculare; cultured cells; coumarins; biosynthesis; quantitative analysis; calcium; alginates; dose response; cell growth
Abstract:
... Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour of S. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics of Solanum aviculare cells immobilised wi ...
DOI:
10.1007/s002990050237

8. Evaluation of five promoters for use in transformation of oil palm (Elaeis guineensis Jacq.)

Author:
Chowdhury, M.K.U.; Parveez, G.K.A.; Saleh, N.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 277-281
ISSN:
0721-7714
Subject:
Elaeis guineensis; Nicotiana tabacum; genetic transformation; gene transfer; beta-glucuronidase; enzyme activity; reporter genes; gene expression; callus; leaves; histochemistry; promoter regions
Abstract:
... The efficiency of GUS (beta-Glucuronidase) gene expression in embryogenic callus and young leaflets of mature and seedling palm after microprojectile bombardment with five constructs (pEmuGN, pAHC25, pAct1-F4, pGH24 and pBARGUS) was evaluated to identify the most suitable promoter(s) to use in transformation attempts in oil palm. Expression of the GUS gene driven by the Emu, Ubi1, Act1, 35S or Adh ...
DOI:
10.1007/s002990050222

9. Expression of the green fluorescent protein gene in conifer tissues

Author:
Tian, L.; Seguin, A.; Charest, P.J.
Source:
Plant cell reports 1997 v.16 no.5 pp. 267-271
ISSN:
0721-7714
Subject:
Picea mariana; Picea glauca; Pinus strobus; Scyphozoa; animal proteins; fluorescence; reporter genes; genetic markers; genetic code; gene transfer; gene expression; pollen; cell suspension culture; embryo (plant); developmental stages; plant anatomy; plasmids
Abstract:
... The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in black spruce pollen. While the wild-type GFP gene resulted in a low level of exp ...
DOI:
10.1007/s002990050220

10. Gemmation in cultured gametophytes of Osmunda regalis

Author:
Fernandez, H.; Bertrand, A.M.; Sanchez-Tames, R.
Source:
Plant cell reports 1997 v.16 no.5 pp. 358-362
ISSN:
0721-7714
Subject:
Osmunda regalis; germ cells; in vitro culture; methodology; culture media; sugars; dose response; pH; light intensity; cell growth; plant development; micropropagation; developmental stages; plant anatomy
Abstract:
... Gametophytes of Osmunda regalis were cultured in vitro to determine the optimal conditions for growth and development. Culture media with low ammonium concentration (Knop, Knudson or 1/4 Murashige and Skoog dilution) were the most effective for growth of this organism. Addition of sucrose and mannitol to the culture medium at the same osmoticum, inhibits or enhances, respectively, growth and devel ...
DOI:
10.1007/s002990050239

11. High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis

Author:
Fiore, M.C.; Trabace, T.; Sunseri, F.
Source:
Plant cell reports 1997 v.16 no.5 pp. 295-298
ISSN:
0721-7714
Subject:
Helianthus annuus; cotyledons; somatic embryogenesis; developmental stages; callus; shoots; methodology; culture media; benzyladenine; gibberellic acid; ethanol; dose response; genetic variation; explants; rooting; naphthaleneacetic acid; genotype
Abstract:
... A plant regeneration method via somatic embryogenesis of several Helianthus annuus L. genotypes was developed. Starting from cotyledonary explants high frequency embryo induction was obtained following several subcultures on defined media. An appropriate cotyledon developmental stage was identified. Etiolated explants and darkness treatment were necessary to obtain somatic embryos in all tested ge ...
DOI:
10.1007/s002990050226

12. In vitro adventitious shoot production in Paulownia

Author:
Bergmann, B.A.; Moon, H.K.
Source:
Plant cell reports 1997 v.16 no.5 pp. 315-319
ISSN:
0721-7714
Subject:
Paulownia; Paulownia fortunei; leaves; petioles; in vitro culture; culture media; benzyladenine; shoots; callus; clones; genetic variation; naphthaleneacetic acid; explants; genotype
Abstract:
... In vitro adventitious shoot formation was examined in petioles and laminae of Paulownia elongata, P. fortunei, and P. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot formation. When single explants from the center of each lamina were cultured, ...
DOI:
10.1007/s002990050230

13. In vitro micropropagation of an endemic fruit tree Syzygium alternifolium (Wight) walp

Author:
Sha Valli Khan, P.S.; Prakash, E.; Rao, K.R.
Source:
Plant cell reports 1997 v.16 no.5 pp. 325-328
ISSN:
0721-7714
Subject:
Syzygium; buds; in vitro culture; micropropagation; culture media; benzyladenine; kinetin; adenine; dose response; rooting; explants; naphthaleneacetic acid; indole acetic acid; indole butyric acid
Abstract:
... Shoot proliferation was obtained from shoot tip and nodal bud explants of Syzygium alterniflorum (Wight) walp on modified Murashige and Skoogs medium (MS) supplemented with either BA, KN or AD alone or BA in combination with either IAA, NAA or IBA. A combination of BA and auxins produced more shoots from both types of explants than on the medium containing only cytokinins. The highest multiplicati ...
DOI:
10.1007/s002990050232

14. Multiple shoot formation from normal and malformed somatic embryo explants of Eastern redbud (Cercis canadensis L.)

Author:
Distabanjong, K.; Geneve, R.L.
Source:
Plant cell reports 1997 v.16 no.5 pp. 334-338
ISSN:
0721-7714
Subject:
Cercis canadensis; embryo culture; embryo (plant); shoots; somatic embryogenesis; abnormal development; culture media; abscisic acid; benzyladenine; dose response; methodology; micropropagation; explants
Abstract:
... A procedure for multiple shoot formation from somatic embryo explants of Eastern redbud (Cercis canadensis L.) cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. TDZ in combination with BA produced more shoots than either treatment alone. The highest number of shoots (3.3 to 3.4 shoots per explant) was obtained from partially desiccated and wounded explants t ...
DOI:
10.1007/s002990050234

15. Pectic enzymes as a selective pressure tool for in vitro recovery of strawberry plants with fungal disease resistance

Author:
Orlando, R.; Magro, P.; Rugini, E.
Source:
Plant cell reports 1997 v.16 no.5 pp. 272-276
ISSN:
0721-7714
Subject:
Fragaria ananassa; Rhizoctonia fragariae; Botrytis cinerea; plant pathogenic fungi; pathogenicity; disease resistance; plant breeding; callus; polygalacturonase; enzyme activity; phenolic compounds; biosynthesis; quantitative analysis
Abstract:
... A novel strategy in selecting strawberry (Fragaria x ananassa L.) plants with resistance to Rhizoctonia fragariae and Botrytis cinerea was developed. Purified pectic enzymes produced by R. fragariae were used in vitro to select morphogenetic calluses. Both regenerated shoots and plants were tested in vitro and in vivo with R. fragariae and B. cinerea. The in vitro resistance of shoots regenerated ...
DOI:
10.1007/s002990050221

16. Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

Author:
Borejsza-Wysocki, W.; Borejsza-Wysocka, E.; Hrazdina, G.
Source:
Plant cell reports 1997 v.16 no.5 pp. 304-309
ISSN:
0721-7714
Subject:
Pisum sativum; cell suspension culture; pisatin; metabolism; biosynthesis; quantitative analysis; chemical reactions; biochemical pathways; phenylalanine ammonia-lyase; oxidoreductases; enzyme activity; plant pathogens; defense mechanisms; disease resistance; naringenin-chalcone synthase
Abstract:
... Cell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased sy ...
DOI:
10.1007/s002990050228

17. Plant regeneration from callus cultures of Lithospermum erythrorhizon

Author:
Yu, H.J.; Oh, S.K.; Oh, M.H.; Choi, D.W.; Kwon, Y.M.; Kim, S.G.
Source:
Plant cell reports 1997 v.16 no.5 pp. 261-266
ISSN:
0721-7714
Subject:
Lithospermum erythrorhizon; callus; cell growth; plant pigments; biosynthesis; methodology; culture media; 2,4-D; picloram; benzyladenine; kinetin; dose response; organogenesis; somatic embryogenesis; cell differentiation; developmental stages; plant morphology; plant anatomy; naphthaleneacetic acid; indole acetic acid
Abstract:
... We previously studied the production of shikonin derivatives by cell lines of Lithospermum erythrorhizon. As a result, we have obtained a cell line LE 87, which exhibited high cell growth and high shikonin production. In the present study, the effects of auxins (2,4-D, IAA, picloram, and NAA) and cytokinins (BAP and kinetin) on organogenesis and somatic embryogenesis in this shikonin-producing cel ...
DOI:
10.1007/s002990050219

18. RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var. indica) somaclonal progenies

Author:
Godwin, I.D.; Sangduen, N.; Kunanuvatchaidach, R.; Piperidis, G.; Adkins, S.W.
Source:
Plant cell reports 1997 v.16 no.5 pp. 320-324
ISSN:
0721-7714
Subject:
Oryza sativa; clones; genetic techniques and protocols; somaclonal variation; genetic markers; genetic polymorphism; cultivars; homozygosity; heterozygosity; recessive genes; phenotype; mutation
Abstract:
... RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar, indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental ...
DOI:
10.1007/s002990050231

19. Selection of Mycosphaerella fijiensis-resistant cell lines from micro-cross sections of banana and plantain

Author:
Okole, B.N.; Schulz, F.A.
Source:
Plant cell reports 1997 v.16 no.5 pp. 339-343
ISSN:
0721-7714
Subject:
Musa; Mycosphaerella fijiensis; plant pathogenic fungi; mycotoxins; pathogenicity; disease resistance; plant breeding; cell lines; cultivars; genetic variation; in vitro culture; shoots; buds; culture media; micropropagation; explants; culture filtrates
Abstract:
... An in vitro selection system using micro-cross sections of banana and plantain cultivars belonging to AAA and A.B. genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin ...
DOI:
10.1007/s002990050235

20. Somatic embryogenesis in Gnetum ula Brongn. (Gnetum edule) (Willd) Blume

Author:
Augustine, A.C.; D'Souza, L.
Source:
Plant cell reports 1997 v.16 no.5 pp. 354
ISSN:
0721-7714
Subject:
Gnetum; somatic embryogenesis; embryo (plant); culture media; benzyladenine; 2,4-D; nitrates; glutamine; sucrose; abscisic acid; dose response; methodology; callus; developmental stages; plant anatomy; endangered species; explants
Abstract:
... Somatic embryos of Gnetum ula (Gnetum edule) an endangered gymnosperm closely related to the angiosperms have been induced in vitro. Megagametophyte tissue with immature embryos was cultured on Murashige and Skoog medium. A mucilaginous, translucent embryogenic callus was obtained with 5 mg/l BA. Callus induced with 2,4-D was non-embryogenic. The embryogenic callus in liquid half strength Murashig ...
DOI:
10.1007/s002990050238