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You searched for: Journal name Plant cell reports Remove constraint Journal name: Plant cell reports Publication year rev 8003-1997 Remove constraint Publication year rev: 8003-1997 Source 1997 v.16 no.5 Remove constraint Source: 1997 v.16 no.5
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1. Chromosome doubling via tuber disc culture in dihaploid potato as determined by confocal microscopy

Author:
Mozafari, J.; Wolyn, D.J.; Ali-Khan, S.T.
Source:
Plant cell reports 1997 v.16 no.5 pp. 329-333
ISSN:
0721-7714
Subject:
guard cells; chloroplasts; in vitro culture; haploidy; chromosome number; developmental stages; Solanum tuberosum; tetraploidy; rapid methods; chromosome addition; tubers; genotype; somaclonal variation; leaves
Abstract:
... The potential of tuber disc culture for chromosome doubling was investigated in somaclonal populations of four dihaploid genotypes and one tetraploid cultivar of potato (Solanum tuberosum). Laser scanning confocal microscopy (LSCM) was used for rapid determination of the ploidy level based on the number of chloroplasts in stomatal guard cells of leaves. Factorial analysis of chloroplast number in ...
DOI:
10.1007/BF01088291

2. Enhanced production of scopolin by Solanum aviculare cells immobilised within Ca-alginate gel beads

Author:
Roisin, C.; Gillet-Manceau, F.; Nava Saucedo, J.E.; Fliniaux, M.; Jacquin-Dubreuil, A.; Barbotin, J.N.
Source:
Plant cell reports 1997 v.16 no.5 pp. 349-353
ISSN:
0721-7714
Subject:
Solanum aviculare; cultured cells; coumarins; biosynthesis; quantitative analysis; calcium; alginates; dose response; cell growth
Abstract:
... Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour of S. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics of Solanum aviculare cells immobilised wi ...
DOI:
10.1007/s002990050237

3. Gemmation in cultured gametophytes of Osmunda regalis

Author:
Fernandez, H.; Bertrand, A.M.; Sanchez-Tames, R.
Source:
Plant cell reports 1997 v.16 no.5 pp. 358-362
ISSN:
0721-7714
Subject:
plant development; in vitro culture; germ cells; Osmunda regalis; sugars; dose response; cell growth; developmental stages; plant anatomy; light intensity; pH; micropropagation; culture media
Abstract:
... Gametophytes of Osmunda regalis were cultured in vitro to determine the optimal conditions for growth and development. Culture media with low ammonium concentration (Knop, Knudson or 1/4 Murashige and Skoog dilution) were the most effective for growth of this organism. Addition of sucrose and mannitol to the culture medium at the same osmoticum, inhibits or enhances, respectively, growth and devel ...
DOI:
10.1007/s002990050239

4. High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis

Author:
Fiore, M.C.; Trabace, T.; Sunseri, F.
Source:
Plant cell reports 1997 v.16 no.5 pp. 295-298
ISSN:
0721-7714
Subject:
Helianthus annuus; dose response; ethanol; developmental stages; gibberellic acid; explants; cotyledons; somatic embryogenesis; rooting; benzyladenine; genotype; naphthaleneacetic acid; genetic variation; culture media; callus; shoots
Abstract:
... A plant regeneration method via somatic embryogenesis of several Helianthus annuus L. genotypes was developed. Starting from cotyledonary explants high frequency embryo induction was obtained following several subcultures on defined media. An appropriate cotyledon developmental stage was identified. Etiolated explants and darkness treatment were necessary to obtain somatic embryos in all tested ge ...
DOI:
10.1007/s002990050226

5. Pectic enzymes as a selective pressure tool for in vitro recovery of strawberry plants with fungal disease resistance

Author:
Orlando, R.; Magro, P.; Rugini, E.
Source:
Plant cell reports 1997 v.16 no.5 pp. 272-276
ISSN:
0721-7714
Subject:
Fragaria ananassa; Rhizoctonia fragariae; Botrytis cinerea; plant pathogenic fungi; pathogenicity; disease resistance; plant breeding; callus; polygalacturonase; enzyme activity; phenolic compounds; biosynthesis; quantitative analysis
Abstract:
... A novel strategy in selecting strawberry (Fragaria x ananassa L.) plants with resistance to Rhizoctonia fragariae and Botrytis cinerea was developed. Purified pectic enzymes produced by R. fragariae were used in vitro to select morphogenetic calluses. Both regenerated shoots and plants were tested in vitro and in vivo with R. fragariae and B. cinerea. The in vitro resistance of shoots regenerated ...
DOI:
10.1007/s002990050221

6. Plant regeneration from callus cultures of Lithospermum erythrorhizon

Author:
Yu, H.J.; Oh, S.K.; Oh, M.H.; Choi, D.W.; Kwon, Y.M.; Kim, S.G.
Source:
Plant cell reports 1997 v.16 no.5 pp. 261-266
ISSN:
0721-7714
Subject:
kinetin; biosynthesis; plant pigments; cell growth; dose response; picloram; developmental stages; culture media; methodology; somatic embryogenesis; plant morphology; benzyladenine; indole acetic acid; naphthaleneacetic acid; cell differentiation; 2,4-D; Lithospermum erythrorhizon; callus; organogenesis
Abstract:
... We previously studied the production of shikonin derivatives by cell lines of Lithospermum erythrorhizon. As a result, we have obtained a cell line LE 87, which exhibited high cell growth and high shikonin production. In the present study, the effects of auxins (2,4-D, IAA, picloram, and NAA) and cytokinins (BAP and kinetin) on organogenesis and somatic embryogenesis in this shikonin-producing cel ...
DOI:
10.1007/s002990050219

7. RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var. indica) somaclonal progenies

Author:
Godwin, I.D.; Sangduen, N.; Kunanuvatchaidach, R.; Piperidis, G.; Adkins, S.W.
Source:
Plant cell reports 1997 v.16 no.5 pp. 320-324
ISSN:
0721-7714
Subject:
cultivars; phenotype; Oryza sativa; heterozygosity; clones; recessive genes; somaclonal variation; genetic polymorphism; homozygosity; genetic markers
Abstract:
... RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar, indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental ...
DOI:
10.1007/s002990050231

8. Selection of Mycosphaerella fijiensis-resistant cell lines from micro-cross sections of banana and plantain

Author:
Okole, B.N.; Schulz, F.A.
Source:
Plant cell reports 1997 v.16 no.5 pp. 339-343
ISSN:
0721-7714
Subject:
cultivars; plant breeding; buds; explants; Musa; pathogenicity; mycotoxins; plant pathogenic fungi; disease resistance; micropropagation; genetic variation; cell lines; Mycosphaerella fijiensis; culture media; culture filtrates; shoots
Abstract:
... An in vitro selection system using micro-cross sections of banana and plantain cultivars belonging to AAA and A.B. genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin ...
DOI:
10.1007/s002990050235

9. Somatic embryogenesis in Gnetum ula Brongn. (Gnetum edule) (Willd) Blume

Author:
Augustine, A.C.; D'Souza, L.
Source:
Plant cell reports 1997 v.16 no.5 pp. 354
ISSN:
0721-7714
Subject:
callus; endangered species; dose response; Gnetum; glutamine; sucrose; embryo (plant); developmental stages; explants; somatic embryogenesis; benzyladenine; abscisic acid; 2,4-D; culture media; nitrates
Abstract:
... Somatic embryos of Gnetum ula (Gnetum edule) an endangered gymnosperm closely related to the angiosperms have been induced in vitro. Megagametophyte tissue with immature embryos was cultured on Murashige and Skoog medium. A mucilaginous, translucent embryogenic callus was obtained with 5 mg/l BA. Callus induced with 2,4-D was non-embryogenic. The embryogenic callus in liquid half strength Murashig ...
DOI:
10.1007/s002990050238

10. Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions

Author:
Dussert, S.; Chabrillange, N.; Anthony, F.; Engelmann, F.; Recalt, C.; Hamon, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 344-348
ISSN:
0721-7714
Subject:
Coffea; germplasm; shelf life; culture media; methodology
Abstract:
... An in vitro core collection of African coffee germplasm, structured in 32 diploid diversity groups, was established and conserved under slow growth for 3 years (6 subcultures). The initial objective was to store twenty accessions per group, with four replicates per accession. A statistical model was developed to analyse observations of survival rates within each diversity group. The goodness of fi ...
DOI:
10.1007/s002990050236

11. Variation of alkaloid productivity among several clones of hairy roots and regenerated plants of Atropa bellandonna transformed with Agrobacterium rhizogenes 15834

Author:
Aoki, T.; Matsumoto, H.; Asako, Y.; Matsunaga, Y.; Shimomura, K.
Source:
Plant cell reports 1997 v.16 no.5 pp. 282-286
ISSN:
0721-7714
Subject:
genetic transformation; biosynthesis; quantitative analysis; cell growth; phenotype; transgenic plants; Rhizobium rhizogenes; clones; Atropa belladonna; atropine; genetic variation; culture media; roots
Abstract:
... Hairy root cultures of Atropa belladonna were established by transformation with Agrobacterium rhizogenes 15834. Five clones of them were employed to study the production of hyoscyamine, the main constituent of the plant, together with other tropane alkaloids. The growth and alkaloid production of each clone were differently affected by basal liquid culture media tested. The transgenic plants rege ...
DOI:
10.1007/BF01088281

12. An efficient particle bombardment system for the genetic transformation of asparagus (Asparagus officinalis L.)

Author:
Cabrera-Ponce, J.L.; Lopez, L.; Assad-Garcia, N.; Medina-Arevalo, C.; Bailey, A.M.; Herrera-Estrella, L.
Source:
Plant cell reports 1997 v.16 no.5 pp. 255-260
ISSN:
0721-7714
Subject:
plasmids; glufosinate; callus; Asparagus officinalis; gene expression; cultivars; genetic code; phosphotransferases (phosphomutases); herbicide resistance; enzyme activity; culture media; histochemistry; genetic transformation; gene transfer; plant breeding; transgenic plants; embryo (plant); genetic variation; beta-glucuronidase; acyltransferases
Abstract:
... The microprojectile bombardment method was used to transfer DNA into embryogenic callus of asparagus (Asparagus officinalis L.) and to produce stably transformed asparagus plants. Embryogenic callus, derived from UC157 and UC72 asparagus cultivars, was bombarded with tungsten particles coated with plasmid DNA that contained genes encoding hygromycin phosphotransferase, phosphinothricin acetyl tran ...
DOI:
10.1007/s002990050218

13. Agrobacterium strain specificity and shooty tumour formation in eucalypt (Eucalyptus grandis X E. urophylla)

Author:
Machado, L. de O.R.; Andrade, G.M. de.; Barrueto Cid, L.P.; Penchel, R.M.; Brasileiro, A.C.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 299-303
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; neoplasms; Agrobacterium radiobacter; strain differences; Rhizobium rhizogenes; pathogenicity; Eucalyptus urophylla; Eucalyptus grandis
Abstract:
... To develop a successful protocol for Agrobacterium-mediated transformation in plants it is essential to determine the most efficient bacterial strain/plant genotype interaction. In the present work, we evaluated the susceptibility of Eucalyptus grandis x E. urophylla to five Agrobacterium rhizogenes and twelve A. tumefaciens wild-type strains. The results showed different degrees of virulence, acc ...
DOI:
10.1007/BF01088285

14. Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspension cultures

Author:
Godoy-Hernandez, G.; Loyola-Vargas, V.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 287-290
ISSN:
0721-7714
Subject:
anthocyanins; Catharanthus roseus; dose response; biosynthesis; aspirin; neoplasms; alkaloids; quantitative analysis; corn starch; furans; cinnamic acid; secondary metabolites; carbon; Aspergillus fumigatus; coumarins; cell lines; culture media; cell suspension culture
Abstract:
... Addition of various concentrations (0.5-20 mM) of acetylsalicylic acid (ASA) to tumor lines of Catharanthus roseus cultivated in vitro and requiring corn starch as carbon source, produced remarkable effects on secondary metabolite production. An increase of 505% total alkaloids per culture (cells plus liquid medium), 1587% total phenolics (liquid medium), 612% total furanocoumarins (liquid medium) ...
DOI:
10.1007/BF01088282

15. Effect of atmospheric pressure on sunflower (Helianthus annuus L.) protoplast division

Author:
Barthou, H.; Briere, C.; Caumont, C.; Petitprez, M.; Kallerhoff, J.; Borin, C.; Souvre, A.; Alibert, G.
Source:
Plant cell reports 1997 v.16 no.5 pp. 310-314
ISSN:
0721-7714
Subject:
protoplasts; turgor; Helianthus annuus; in vitro culture; biosynthesis; hypocotyls; cell walls; developmental stages; cell division; cytoskeleton; culture media; atmospheric pressure
Abstract:
... Sunflower protoplasts were cultured in liquid medium under high atmospheric pressure (0.2 to 0.6 MPa) and the plating efficiency, cell wall synthesis and microtubule organization were assessed. In 7-day-old cultures under a pressure of 0.4 MPa and above, the division rate was strongly reduced by more than 60% as compared to the control. Although most of the protoplasts had begun to regenerate a ne ...
DOI:
10.1007/s002990050229

16. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

17. Evaluation of five promoters for use in transformation of oil palm (Elaeis guineensis Jacq.)

Author:
Chowdhury, M.K.U.; Parveez, G.K.A.; Saleh, N.M.
Source:
Plant cell reports 1997 v.16 no.5 pp. 277-281
ISSN:
0721-7714
Subject:
Elaeis guineensis; Nicotiana tabacum; genetic transformation; gene transfer; beta-glucuronidase; enzyme activity; reporter genes; gene expression; callus; leaves; histochemistry; promoter regions
Abstract:
... The efficiency of GUS (beta-Glucuronidase) gene expression in embryogenic callus and young leaflets of mature and seedling palm after microprojectile bombardment with five constructs (pEmuGN, pAHC25, pAct1-F4, pGH24 and pBARGUS) was evaluated to identify the most suitable promoter(s) to use in transformation attempts in oil palm. Expression of the GUS gene driven by the Emu, Ubi1, Act1, 35S or Adh ...
DOI:
10.1007/s002990050222

18. Expression of the green fluorescent protein gene in conifer tissues

Author:
Tian, L.; Seguin, A.; Charest, P.J.
Source:
Plant cell reports 1997 v.16 no.5 pp. 267-271
ISSN:
0721-7714
Subject:
gene transfer; genetic code; animal proteins; Picea mariana; fluorescence; embryo (plant); developmental stages; plasmids; gene expression; Pinus strobus; Picea glauca; Scyphozoa; reporter genes; pollen; cell suspension culture; genetic markers
Abstract:
... The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in black spruce pollen. While the wild-type GFP gene resulted in a low level of exp ...
DOI:
10.1007/s002990050220

19. In vitro adventitious shoot production in Paulownia

Author:
Bergmann, B.A.; Moon, H.K.
Source:
Plant cell reports 1997 v.16 no.5 pp. 315-319
ISSN:
0721-7714
Subject:
callus; explants; Paulownia fortunei; clones; benzyladenine; genotype; naphthaleneacetic acid; petioles; genetic variation; culture media; shoots
Abstract:
... In vitro adventitious shoot formation was examined in petioles and laminae of Paulownia elongata, P. fortunei, and P. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot formation. When single explants from the center of each lamina were cultured, ...
DOI:
10.1007/s002990050230

20. In vitro micropropagation of an endemic fruit tree Syzygium alternifolium (Wight) walp

Author:
Sha Valli Khan, P.S.; Prakash, E.; Rao, K.R.
Source:
Plant cell reports 1997 v.16 no.5 pp. 325-328
ISSN:
0721-7714
Subject:
kinetin; buds; adenine; dose response; indole butyric acid; explants; rooting; benzyladenine; micropropagation; Syzygium; naphthaleneacetic acid; culture media; indole acetic acid
Abstract:
... Shoot proliferation was obtained from shoot tip and nodal bud explants of Syzygium alterniflorum (Wight) walp on modified Murashige and Skoogs medium (MS) supplemented with either BA, KN or AD alone or BA in combination with either IAA, NAA or IBA. A combination of BA and auxins produced more shoots from both types of explants than on the medium containing only cytokinins. The highest multiplicati ...
DOI:
10.1007/s002990050232