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You searched for: Journal Plant cell reports Remove constraint Journal: Plant cell reports Publication Year 1996 Remove constraint Publication Year: 1996 Source 1996 v.15 no.11 Remove constraint Source: 1996 v.15 no.11
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1. Androgenesis of the salt tolerant shrub Atriplex glauca

Author:
Kenny, L.; Caligari, P.D.S.
Source:
Plant cell reports 1996 v.15 no.11 pp. 829-832
ISSN:
0721-7714
Subject:
developmental stages; micropropagation; 2,4-D; organogenesis; shoots; diploidy; explants; isopentenyladenine; Atriplex; kinetin; haploidy; androgenesis; genetic improvement; culture media; indole acetic acid; salt tolerance; dosage; flowers; cultured cells; plant morphology
Abstract:
... Clusters of flowers, 4 to 5 days old, were successfully cultured on Nitsch and Nitsch (1969) medium in the presence of growth regulators. An average of 20 plants per explant were produced after 6 weeks. Organized growth was obtained only from flowers collected during spring or early summer. Microscopic observations during shoot regeneration indicated that shoot organogenesis took place directly fr ...
DOI:
10.1007/BF00233149
PubMed:
24178217

2. Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)

Author:
Toldi, O.; Gyulai, G.; Kiss, J.; Tamas, I.A.; Balazs, E.
Source:
Plant cell reports 1996 v.15 no.11 pp. 851-854
ISSN:
0721-7714
Subject:
cultivars; morphogenesis; gibberellic acid; genetic variation; explants; benzyladenine; sodium chloride; micropropagation; epicotyls; charcoal; indole acetic acid; culture media; Beta vulgaris; shoots
Abstract:
... An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole ...
DOI:
10.1007/BF00233155
PubMed:
24178223

3. Cannabis 25, biotransformation of cannabidiol and cannabidiolic acid by Pinellia ternata tissue segments

Author:
Tanaka, H.; Takahashi, R.; Morimoto, S.; Shoyama, Y.
Source:
Plant cell reports 1996 v.15 no.11 pp. 819-823
ISSN:
0721-7714
Subject:
metabolism; quantitative analysis; Pinellia ternata; drugs; tubers; culture filtrates; spectral analysis; hydroxylation; cannabidiol; synthesis; chemical structure; metabolites; tissue culture
Abstract:
... Two marihuana constituents, cannabidiol (1) and cannabidiolic acid (4) were each converted mainly to two metabolites using tissue segments of Pinellia ternata tuber. The structures of the metabolites formed from 1 were determined to be 1-O-beta-D-glucopyranoside and 1-O- beta-D-diglucopyranoside by 1H nmr, 13C nmr and fabms. Those from 4 were determined as 4-O-beta-D-glucopyranoside and 10'-hydrox ...
DOI:
10.1007/BF00233147

4. Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas)

Author:
Blakesley, D.; Al Mazrooei, S.; Bhatti, M.H.; Henshaw, G.G.
Source:
Plant cell reports 1996 v.15 no.11 pp. 873-876
ISSN:
0721-7714
Subject:
germplasm; dose response; cryopreservation; sucrose; embryo (plant); shoots; encapsulation; Ipomoea batatas; benzyladenine; 2,4-D; culture media; tissue culture
Abstract:
... Embryogenic tissue of the sweet potato (Ipomoea batatas (L) LAM) genotype TIB 10 was established from in vitro axillary shoot tips on Murashige and Skoog (1962) medium supplemented with 5 micromolars 2,4-dichlorophenoxyacetic acid. Embryogenic aggregates of fresh mass 9.0-12 mg were subjected to a rapid freezing protocol in liquid nitrogen following sucrose preculture and varying degrees of dehydr ...
DOI:
10.1007/BF00233160
PubMed:
24178228

5. Direct somatic embryogenesis and plant regeneration from single cell suspension cultures of Elymus giganteus Vahl

Author:
Wang, L.; Wang, X.; Huang, B.
Source:
Plant cell reports 1996 v.15 no.11 pp. 865-868
ISSN:
0721-7714
Subject:
kinetin; dose response; embryo (plant); developmental stages; explants; somatic embryogenesis; benzyladenine; Elymus; micropropagation; naphthaleneacetic acid; 2,4-D; culture media; cell suspension culture; callus
Abstract:
... A yellowish, nodular callus was induced from mature embryos of Elymus giganteus Vahl on MS medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l kinetin, from which a cell suspension culture was initiated in liquid MS medium supplemented with 0.5 mg/l 2,4-D, 1.0 mg/l kinetin and 0.2 mg/l naphthaleneacetic acid (NAA). By filtering through a series of sieves with decreasing ...
DOI:
10.1007/BF00233158
PubMed:
24178226

6. DNA methylation and Dc8-GUS transgene expression in carrot (Daucus carota L.)

Author:
Zhou, Y.; Magill, J.M.; Magill, C.W.; Newton, R.J.
Source:
Plant cell reports 1996 v.15 no.11 pp. 815-818
ISSN:
0721-7714
Subject:
Daucus carota; DNA methylation; transgenic plants; gene expression; genes; gene transfer; chimerism; beta-glucuronidase; enzyme activity; cytidine; abscisic acid; dose response; quantitative analysis
Abstract:
... DNA methylation has been associated with gene activity in differentiating and developing plant tissues. The objective of this study was to determine the involvement of methylation in the expression of a gene transferred into carrot (Daucus carota L.) tissues by particle bombardment. Expression of the Dc8-GUS gene construct in response to treatments with 5-azacytidine (5-azaC) and to in vitro methy ...
DOI:
10.1007/BF00233146
PubMed:
24178214

7. Effect of promoter-leader sequences on transient expression of reporter gene chimerase biolistically transferred into sugarbeet (Beta vulgaris) suspension cells

Author:
Ingersoll, J.C.; Heutte, T.M.; Owens, L.D.
Source:
Plant cell reports 1996 v.15 no.11 pp. 836-840
ISSN:
0721-7714
Subject:
biolistics; beta-glucuronidase; chimerism; gene expression; reporter genes; enzyme activity; promoter regions; cell suspension culture; Beta vulgaris
Abstract:
... Chimeric constructs consisting of the gus coding region fused downstream of promoter-untranslated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells. Each construct was expressed in recipient cells at 6 h after bombardment with ma ...
DOI:
10.1007/BF00233152

8. Ferricyanide reductase of rose plasma membranes is regulated by nitrogen supply

Author:
Hu, P.; Murphy, T.M.
Source:
Plant cell reports 1996 v.15 no.11 pp. 833-835
ISSN:
0721-7714
Subject:
Rosa damascena; ions; nitrogen; nutrient availability; oxidoreductases; plasma membrane; enzyme activity; culture media; cell suspension culture; cyanides
Abstract:
... The ability of suspension-cultured rose (Rosa damascena Mill. cv Gloire de Guilan) cells to reduce ferricyanide is decreased by 50% during an overnight incubation in a low-nutrient (1 mM CaCl2, 0.1 mM KCl) solution. This loss is not observed when nitrate and/or glutamate is added to the low-nutrient medium, but it occurs in medium containing all the components needed for normal growth except nitra ...
DOI:
10.1007/BF00233150
PubMed:
24178218

9. In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil)

Author:
Pattnaik, S.; Chand, P.K.
Source:
Plant cell reports 1996 v.15 no.11 pp. 846-850
ISSN:
0721-7714
Subject:
seasonal variation; benzyladenine; micropropagation; 2,4-D; shoots; dose response; Ocimum tenuiflorum; gibberellic acid; explants; length; naphthaleneacetic acid; buds; quantitative analysis; rooting; culture media; indole acetic acid; Ocimum americanum; branching; indole butyric acid
Abstract:
... A procedure is outlined for in vitro propagation of two medicinal herbs, Ocimum americanum L. syn. O. canum Sims (hoary basil) and Ocimum sanctum L. (holy basil), using axillary shoot buds. Multiple shoot formation was induced from shoot bud explants of both species on Murashige and Skoog medium (MS) supplemented with benzyladenine (BA). The optimum BA concentrations for shoot proliferation were 0 ...
DOI:
10.1007/BF00233154
PubMed:
24178222

10. Micropropagation of a fruit tree, Morus australis Poir. syn. M. acidosa Griff

Author:
Pattnaik, S.K.; Sahoo, Y.; Chand, P.K.
Source:
Plant cell reports 1996 v.15 no.11 pp. 841-845
ISSN:
0721-7714
Subject:
apical meristems; kinetin; buds; dose response; gibberellic acid; explants; indole butyric acid; rapid methods; propionic acid; dormancy breaking; benzyladenine; micropropagation; rooting; naphthaleneacetic acid; Morus australis; culture media; indole acetic acid; shoots
Abstract:
... High frequency bud break and multiple shoots were induced in nodal explants collected between November to February from a 5 year old tree of Morus australis Poir syn. M. acidosa Griff. on Murashige and Skoog's medium supplemented with 6-benzylaminopurine (1.0 mg/l). Incorporation of gibberellic acid (0.3 mg/l) along with BAP (1.0 mg/l) not only induced faster bud break from nodal explants as well ...
DOI:
10.1007/BF00233153
PubMed:
24178221

11. Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis

Author:
Jethwani, V.; Kothari, S.L.
Source:
Plant cell reports 1996 v.15 no.11 pp. 869-872
ISSN:
0721-7714
Subject:
Dianthus chinensis; leaves; cell culture; organogenesis; cultured cells; phenylacetic acid; shoots; callus; culture media; 2,4-D; benzyladenine; dose response; micropropagation; naphthaleneacetic acid
Abstract:
... Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or ...
DOI:
10.1007/BF00233159
PubMed:
24178227

12. Protoplasts from Phytolacca dodecandra L'Herit (endod) and P. americana L. (pokeweed)

Author:
Koch, P.E.; Bonness, M.S.; Lu, H.; Mabry, T.J.
Source:
Plant cell reports 1996 v.15 no.11 pp. 824-828
ISSN:
0721-7714
Subject:
protoplasts; protein composition; ribosomes; Phytolacca americana; cell suspension culture; leaves
Abstract:
... Pokeweed (Phytolacca americana L.) and endod (P. dodecandra L'Herit) produce ribosome-inactivating proteins which are sequestered in leaf cell walls. These proteins display strong antiviral activity. To aid in studying the antiviral mechanism, we developed protocols to isolate protoplasts from suspension culture cells and leaves. Ninety-five percent of pokeweed or endod culture cells were converte ...
DOI:
10.1007/BF00233148
PubMed:
24178216

13. Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)

Author:
Rajasekaran, K.
Source:
Plant cell reports 1996 v.15 no.11 pp. 859-864
ISSN:
0721-7714
Subject:
germplasm; cultivars; insect pests; dose response; cryopreservation; Gossypium hirsutum; dimethyl sulfoxide; cryoprotectants; herbicide resistance; plant morphology; pest resistance; cell suspension culture; callus
Abstract:
... Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slo ...
DOI:
10.1007/BF00233157
PubMed:
24178225

14. Screening for stable transformants and stability of beta-glucuronidase gene expression in suspension cultured cells of tall fescue (Festuca arundinacea)

Author:
Kuai, B.; Morris, P.
Source:
Plant cell reports 1996 v.15 no.11 pp. 804-808
ISSN:
0721-7714
Subject:
Festuca arundinacea; genetic transformation; cell suspension culture; transgenic plants; gene expression; genetic stability; clones; genetic variation; beta-glucuronidase; enzyme activity; callus; cytidine; genomics
Abstract:
... By screening cell colonies derived from protoplasts of tall fescue (Festuca arundinacea), transformed with a rice actin-1-promoter-beta-glucuronidase gene construct, several beta-glucuronidase positive callus clones were obtained. Two callus clones with different GUS expression were derived from these. One was light blue after X-gluc staining, and expression of the beta-glucuronidase gene was stab ...
DOI:
10.1007/BF00233144

15. Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica

Author:
Garg, L.; Bhandari, N.N.; Rani, V.; Bhojwani, S.S.
Source:
Plant cell reports 1996 v.15 no.11 pp. 855-858
ISSN:
0721-7714
Subject:
germination; dose response; glutamine; embryo (plant); triploidy; developmental stages; plant extracts; somatic embryogenesis; endosperm; cultured cells; benzyladenine; coconuts; plant morphology; cell differentiation; Vachellia nilotica; 2,4-D; culture media; callus
Abstract:
... Immature endosperm of Acacia nilotica formed a nodular callus on MS medium supplemented with 2,4-D, BAP and CH. In the third passage on this medium, in the dark the callus differentiated somatic embryos. The embryos germinated on MS only after 15 d pre-treatment on modified MS medium in which major salts were replaced by those of major salts of B5 medium and supplemented with glutamine CH and CW. ...
DOI:
10.1007/BF00233156
PubMed:
24178224

16. Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants

Author:
Nishibayashi, S.; Kaneko, H.; Hayakawa, T.
Source:
Plant cell reports 1996 v.15 no.11 pp. 809-814
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; hygromycin B; hypocotyls; Cucumis sativus; transgenic plants; Agrobacterium radiobacter; developmental stages; explants; culture media; plant morphology; genes; enzyme activity; syringic acid; genetic vectors; beta-glucuronidase; acetosyringone
Abstract:
... Transgenic cucumber (Cucumis sativus L.) plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with NOS-nptII, CaMV 35S-I-gus and CaMV 35S-hph genes. Acetosyringone enhanced the efficiency of transformation at the cut surface cells of hypocotyl explants during five days of co-cultivation. Transformed cells were m ...
DOI:
10.1007/BF00233145
PubMed:
24178213

17. Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

Author:
Rashid, H.; Toriyama, K.; Hinata, K.
Source:
Plant cell reports 1996 v.15 no.11 pp. 799-803
ISSN:
0721-7714
Subject:
hygromycin B; flowering; genes; benzyladenine; Moricandia arvensis; callus; shoots; dose response; Agrobacterium radiobacter; explants; naphthaleneacetic acid; leaves; enzyme activity; culture media; genetic transformation; gene transfer; kanamycin; transgenic plants; internodes; cell suspension culture; genetic vectors; beta-glucuronidase
Abstract:
... A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and beta-glucuronidase (GUS). Max ...
DOI:
10.1007/BF00233143
PubMed:
24178211