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You searched for: Journal name Plant molecular biology Remove constraint Journal name: Plant molecular biology Publication year rev 8013-1987 Remove constraint Publication year rev: 8013-1987 Source 1987 v.9 no.6 Remove constraint Source: 1987 v.9 no.6
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1. Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes

Author:
Hamill, J.D.; Prescott, A.; Martin, C.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 573-584
ISSN:
0167-4412
Subject:
genetic transformation; hygromycin B; kanamycin; Agrobacterium radiobacter; Nicotiana rustica; Rhizobium rhizogenes; Phaseolus vulgaris; plasmids; secondary metabolites; roots; Beta vulgaris; drug resistance
Abstract:
... Co-transfer of Agrobacterium rhizogenes T-DNA and T-DNA from the A. tumefaciens binary vector pBin19 (Bevan, 1984) was studied in detail using Nicotiana rustica. High frequencies of co-transfer of T-DNA's were observed, even when no selection pressure was exerted. Increased levels of pBin19 T-DNA were found in hairy root cultures with selection at higher levels of kanamycin sulphate (50-200 microg ...
DOI:
10.1007/BF00020534
PubMed:
24277194

2. Characterization of a class of small auxin-inducible soybean polyadenylated RNAs

Author:
McClure, B.A.; Guilfoyle, T.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 611-623
ISSN:
0167-4412
Subject:
Glycine max; RNA; gene expression; 2,4-D; auxins; hypocotyls; epicotyls; heat stress; cadmium; cycloheximide; DNA; fusicoccin
Abstract:
... Four new auxin-responsive RNAs from soybean (Glycine max (L.) Merr., var. Wayne) are described. The RNAs were identified by hybridization to three cDNA probes obtained from a library enriched for sequences which increase in abundance within 60 min after 2,4-D (2,4-dichlorophenoxyacetic acid) treatment. These RNAs appear to define a new class of small (i.e. approximately 550 nucleotides) RNAs that ...
DOI:
10.1007/BF00020537
PubMed:
24277197

3. Expression of nodule-specific genes in Phaseolus vulgaris L

Author:
Campos, F.; Padilla, J.; Vazquez, M.; Ortega, J.L.; Enriquez, C.; Sanchez, F.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 521-532
ISSN:
0167-4412
Subject:
messenger RNA; nodulins; root nodules; Phaseolus vulgaris; gene expression; Rhizobium phaseoli; oxidoreductases; leghemoglobin; organogenesis
Abstract:
... The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, nodule-specific uricase and a group that in vitro translates into a cluster of ab ...
DOI:
10.1007/BF00020530
PubMed:
24277190

4. Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line

Author:
David, C.; Tempe, J.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 585-592
ISSN:
0167-4412
Subject:
genetic transformation; plant diseases and disorders; opines; phenotype; Daucus carota; Rhizobium rhizogenes; plasmids; inheritance (genetics); roots; organogenesis
Abstract:
... We have analyzed opine content, T-DNA content, and developmental features of one hairy root culture line and its progeny. Opine-positive progeny still have the hairy, root phenotype and have essentially the same T-DNA structure as the parental plant. Opineless progeny do not exhibit the hairy root phenotype. Some of them do not contain any T-DNA sequences, whereas others contain only the left part ...
DOI:
10.1007/BF00020535
PubMed:
24277195

5. Expression of a gene for a light-harvesting chlorophyll a/b-binding protein in Chlamydomonas reinhardtii: effect of light and acetate

Author:
Kindle, K.L.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 547-563
ISSN:
0167-4412
Subject:
temperature; messenger RNA; light harvesting complex; sodium acetate; heat stress; gene expression; blue light; diuron; mutants; red light; Chlamydomonas reinhardtii; photosynthesis; multigene family
Abstract:
... In Chlamydomonas reinhardtii, the chlorophyll a/b-binding proteins of photosystem II are encoded in the nucleus by, a small family of genes. We have studied the expression of one gene, which we call cabII-1, in a green-in-the-dark strain, which can synthesize chlorophyll in the dark or light, and in a yellow-in-the-dark mutant strain, which is able to make chlorophyll only in the light. In light/d ...
DOI:
10.1007/BF00020532
PubMed:
24277192

6. Identification, purification, and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves

Author:
Loon, L.C. van.; Gerritsen, Y.A.M.; Ritter, C.E.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 593-609
ISSN:
0167-4412
Subject:
Tobacco mosaic virus; immunochemistry; amino acids; Nicotiana tabacum; leaves; amino acid composition; molecular weight; pathogenesis-related proteins; infection
Abstract:
... Ten pH-3 soluble, low-molecular-weight pathogenesis-related proteins (PRs) were found to accumulate in leaves of tobacco cv: Samsun NN reacting hypersensitively to tobacco mosaic virus. Besides the previously characterized PRs 1a, 1b, 1c and 2, these proteins were provisionally designated N, O, P, Q, R, and S in order of decreasing electrophoretic mobility in native polyacrylamide gels. Two-dimens ...
DOI:
10.1007/BF00020536
PubMed:
24277196

7. An auxin-induced polypeptide in dicotyledonous plants

Author:
Wright, R.M.; Hagen, G.; Guilfoyle, T.
Source:
Plant molecular biology 1987 v.9 no.6 pp. 625-634
ISSN:
0167-4412
Subject:
protein synthesis; Glycine max; hypocotyls; immunohistochemistry; auxins; epicotyls; 2,4-D; roots; cell suspension culture; plant proteins
Abstract:
... Antisera were raised to a 70-kD (kilodalton) soybean (Glycine max) protein encoded by a 2,4-dichlorophenoxyacetic acid (2,4-D) inducible mRNA, GH3. These antisera have been used to probe protein blots to study the kinetics and specificity of the GH3 induction response as well as the species specificity and intracellular location of the protein. Detectable levels of the GH3 protein are induced by 2 ...
DOI:
10.1007/BF00020538
PubMed:
24277198