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- Author:
- He, Lin; Gao, Caiqiu; Wang, Yucheng; Wu, Yingjie; Liu, Zhihua
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 344-351
- ISSN:
- 0735-9640
- Subject:
- transcription factors; osmotic stress; mutation; transcription (genetics); plant veins; genes; gene fusion; leucine zipper; abscisic acid; Populus; promoter regions; roots; leaves
- Abstract:
- ... Basic helix-loop-helix (bHLH) transcription factors (TF) comprise a large group of proteins that are involved in many developmental and physiological processes in plants. In this study, a bHLH gene (PkbHLH2), along with its promoter, was cloned from Populus koreana Rehd. A PkbHLH2 promoter::GUS gene fusion construct was generated to investigate the expression of PkbHLH2. The results demonstrated t ...
- DOI:
- 10.1007/s11105-012-0507-6
-
http://dx.doi.org/10.1007/s11105-012-0507-6
- Author:
- Haq, Noor Ul; Raza, Sana; Luthe, Dawn S.; Heckathorn, Scott A.; Shakeel, Samina N.
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 398-408
- ISSN:
- 0735-9640
- Subject:
- Chenopodium album; cadmium; gene expression; heat; heat shock proteins; heat stress; heat tolerance; photosystem II; thylakoids
- Abstract:
- ... The chloroplast small heat shock proteins (Cp-sHSP) protect photosystem II and thylakoid membranes during heat and other types of stresses. The amount of Cp-sHSPs produced is correlated with plant thermotolerance. Cp-sHSPs of Chenopodium album have already been shown to be heat-regulated and have a novel promoter architecture containing some conserved regulatory motifs Shakeel et al. (Plant Physio ...
- DOI:
- 10.1007/s11105-012-0516-5
-
http://dx.doi.org/10.1007/s11105-012-0516-5
- Author:
- Li, Xiao-Fang; Xu, Jing; Yang, Ru; Jia, Lin-Yan; Deng, Xin-Jie; Xiong, Li-Jun; Zhang, Xue-Ping; Fang, Qi; Zhang, Wei; Sun, Yue; Xu, Ling
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 255-263
- ISSN:
- 0735-9640
- Subject:
- Arabidopsis; Narcissus tazetta; calyx; clones; genes; ovules; phenotype; stamens; transgenic plants; yeasts
- Abstract:
- ... Very few flower organ identity genes have been characterized in Chinese narcissus (Narcissus tazetta var. chinensis), which has petaloid sepals. Here, we report the cloning of two full-length B-class genes, namely NAP3L3 and NAP3L4, that are orthologs of the DEFICIENS lineage. Both genes are highly expressed in the second whorl of the perianth and in the stamens. NAP3L4 is also expressed strongly ...
- DOI:
- 10.1007/s11105-012-0493-8
-
http://dx.doi.org/10.1007/s11105-012-0493-8
- Author:
- Ovesná, J.; Kučera, L.; Vaculová, K.; Milotová, J.; Snape, J.; Wenzl, P.; Huttner, E.; Kilian, A.; Martelli, G.; Milella, L.
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 280-288
- ISSN:
- 0735-9640
- Subject:
- DNA; alleles; barley; cultivars; field experimentation; gene frequency; genetic background; genetic markers; genetic variation; malting barley; principal component analysis
- Abstract:
- ... Analysis of the extent of genetic variation within genetic resources is important for diversity preservation and also for breeders who exploit it. We investigated the recently introduced molecular marker technique of DNA diversity array technology (DArT), with the objective of characterising diversity in the likely relatively narrow genetic background of Czech malting barley cultivars. A total of ...
- DOI:
- 10.1007/s11105-012-0491-x
-
http://dx.doi.org/10.1007/s11105-012-0491-x
- Author:
- Yu, Xiu-Mei; Zhao, Wei-Quan; Yang, Wen-Xiang; Liu, Fen; Chen, Jia-Ping; Goyer, Claudia; Liu, Da-Qun
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 314-322
- ISSN:
- 0735-9640
- Subject:
- barley; seeds; stems; open reading frames; polyclonal antibodies; recombinant proteins; DNA; corn; wheat; Western blotting; gene expression; leaf rust; pathogens; DNA fingerprinting; Puccinia recondita; exons; roots; leaves
- Abstract:
- ... Hypersensitive-induced reaction (HIR) proteins and more specific members of the proliferation, ion and death superfamily participate in response to pathogen attacks and development of spontaneous hypersensitive lesions in maize and barley leaves. In the present study, a full-length TaHIR3 (1,246-bp) was cloned and characterized from wheat near-isogenic line Thatcher-Lr15 infected by Puccinia triti ...
- DOI:
- 10.1007/s11105-012-0504-9
-
http://dx.doi.org/10.1007/s11105-012-0504-9
- Author:
- Fanjardo, D.; Morales, J.; Zhu, H.; Steffan, S.; Harbur, R.; Bassil, N.; Hummer, K.; Polashock, J.; Vorsa, N.; Zalapa, J.
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 264
- ISSN:
- 0735-9640
- Subject:
- Vaccinium macrocarpon; alleles; cluster analysis; cranberries; cultivars; environmental factors; fruit crops; genetic heterogeneity; genetic markers; hybrids; indigenous species; microsatellite repeats; parents; progeny
- Abstract:
- ... Cranberries (Vaccinium macrocarpon Ait.) are an economically important fruit crop derived from a North American native species. We report the application of 12 simple sequence repeats (SSR) or microsatellite markers to assess the genetic diversity of cranberry cultivars. We studied 164 samples of 21 different cranberry cultivars, 11 experimental hybrids, and 6 representative accessions of wild spe ...
- Handle:
- 10113/55930
- DOI:
- 10.1007/s11105-012-0497-4
-
http://dx.doi.org/10.1007/s11105-012-0497-4
- Author:
- Manohar, Sumanth; Jagadeeswaran, Guru; Nimmakayala, Padma; Tomason, Yan; Almeida, Aldo; Sunkar, Ramanjulu; Levi, Amnon; Reddy, Umesh K.
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 335-343
- ISSN:
- 0735-9640
- Subject:
- Cucurbita moschata; Cucurbita pepo; Luffa acutangula; Momordica charantia; RNA libraries; fruits; genes; leaves; microRNA; pumpkins; squashes; stems
- Abstract:
- ... MicroRNA genes (miRNAs) encoding small non-coding RNAs are abundant in plant genomes and play a key role in regulating several biological mechanisms. Five conserved miRNAs, miR156, miR168-1, miR168-2, miR164, and miR166 were selected for analysis from the 21 known plant miRNA families that were recovered from deep sequencing data of small RNA libraries of pumpkin and squash. A total of six novel m ...
- DOI:
- 10.1007/s11105-012-0506-7
-
http://dx.doi.org/10.1007/s11105-012-0506-7
- Author:
- Liu, Yukun; Wang, Li; Zhang, Dan; Li, Dequan
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 457-463
- ISSN:
- 0735-9640
- Subject:
- abscisic acid; alternative splicing; corn; gene duplication; genes; leaves; mitogen-activated protein kinase; roots; sodium chloride; stems
- Abstract:
- ... Gene duplication and alternative splicing (AS) are two evolutionary mechanisms that can increase functional diversification of genes. Here, we found that a previously uncharacterized ZmMPK4 (ZmMPK3-1b in this research) is a splicing variant. ZmMPK3-1 can undergo AS by retaining the third intron (90 nucleotides) to generate an atypical mitogen-activated protein kinase (MAPK) gene: ZmMPK3-1b. Furthe ...
- DOI:
- 10.1007/s11105-012-0489-4
-
http://dx.doi.org/10.1007/s11105-012-0489-4
- Author:
- Hong, Yong-bo; Liu, Shui-ping; Zhu, Yan-ping; Xie, Chao; Jue, Deng-wei; Chen, Min; Kaleri, Hubdar Ali; Yang, Qing
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 418-424
- ISSN:
- 0735-9640
- Subject:
- Phytophthora infestans; Ralstonia solanacearum; Southern blotting; Xenopus laevis; antimicrobial peptides; disease resistance; frogs; pathogens; polymerase chain reaction; potatoes; secretion; synthetic genes; transgenic plants
- Abstract:
- ... Magainins are a class of antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. MSI-99 is a synthesized magainin II analogue with high inhibitory effects to a wide spectrum of microbial organisms, including bacteria, fungi and viruses. To verify the resistance conferred by the MSI-99m gene (a MSI-99 synthetic gene with codon usage adapted for expression in ...
- DOI:
- 10.1007/s11105-012-0503-x
-
http://dx.doi.org/10.1007/s11105-012-0503-x
- Author:
- Guo, Dong-wei; Min, Dong-hong; Xu, Zhao-shi; Chen, Ming; Li, Lian-cheng; Ashraf, Muhammad; Ghafoor, Abdul; Ma, You-zhi
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 289-295
- ISSN:
- 0735-9640
- Subject:
- DNA; DNA libraries; Dasypyrum villosum; chromosome addition; chromosomes; fluorescence in situ hybridization; homogenizers; karyotyping; metaphase; root tips; trifluralin; wheat
- Abstract:
- ... To construct a chromosome-specific DNA library of chromosome 6VS from Haynaldia villosa, a wheat alien chromosome addition line T240 with a 6VS chromosome arm and its parental common wheat cv. CA921 were used to optimize protocols for preparing chromosome suspension amenable to flow sorting of the 6VS chromosome. Our results showed that root tips incubated sequentially in Hogland’s solution contai ...
- DOI:
- 10.1007/s11105-012-0492-9
-
http://dx.doi.org/10.1007/s11105-012-0492-9
- Author:
- Fan, Guizhi; Zhai, Qiaoli; Zhan, Yaguang
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 296-302
- ISSN:
- 0735-9640
- Subject:
- biosynthesis; sodium azide; nitric oxide; Betula pendula subsp. mandshurica; nitrate reductase; biochemical pathways; gene expression; esters; cell suspension culture; Diaporthe
- Abstract:
- ... The current work aimed to characterize the generation of nitric oxide (NO) and gene expression of lupeol synthase (LUS) in Betula platyphylla cells exposed to a Phomopsis elicitor. The effects of nitrate reductase (NR) and NO synthase (NOS), the two key enzymes responsible for endogenous NO biosynthesis in plants, were also investigated. NO production in B. platyphylla cell cultures exhibited a bi ...
- DOI:
- 10.1007/s11105-012-0501-z
-
http://dx.doi.org/10.1007/s11105-012-0501-z
- Author:
- Li, Jinhua; Sima, Wei; Ouyang, Bo; Luo, Zhidan; Yang, Changxian; Ye, Zhibiao; Li, Hanxia
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 409-417
- ISSN:
- 0735-9640
- Subject:
- apical meristems; tomatoes; messenger RNA; transcription factors; transcriptional activation; Solanum pennellii; stress response; gel electrophoresis; yeasts; amino acid sequences; heat stress; genes; abscisic acid; cell cycle; zinc finger motif
- Abstract:
- ... The mammalian zinc finger protein ZPR1 is known to be essential in cell cycle progression, but its homolog in plants remains to be identified. Here, we isolated SpZPR1 from the wild tomato species Solanum pennellii LA0716 and found that its protein sequence shares homology with ZPR1 genes from other organisms. SpZPR1 mRNA was found to accumulate in tomato apical meristem in response to ABA (abscis ...
- DOI:
- 10.1007/s11105-012-0509-4
-
http://dx.doi.org/10.1007/s11105-012-0509-4
- Author:
- Nawrot, Robert; Tomaszewski, Łukasz; Czerwoniec, Anna; Goździcka-Józefiak, Anna
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 470-476
- ISSN:
- 0735-9640
- Subject:
- Chelidonium majus; DNA; DNA primers; RNA; RNA-binding proteins; amino acids; cell wall components; crystal structure; models; nucleotide sequences; plant proteins; polymerase chain reaction; protein-protein interactions; sequence analysis
- Abstract:
- ... The family of glycine-rich plant proteins (GRPs) is a large and complex group of proteins that share, as a common feature, the presence of glycine-rich domains arranged in (Gly)n-X repeats that are suggested to be involved in protein–protein interactions, RNA binding, and nucleolar targeting. These proteins are implicated in several independent physiological processes. Some are components of cell ...
- DOI:
- 10.1007/s11105-012-0510-y
- PubMed:
- 24415842
- PubMed Central:
- PMC3881573
-
http://dx.doi.org/10.1007/s11105-012-0510-y
- Author:
- Unver, Turgay; Turktas, Mine; Budak, Hikmet
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 323-334
- ISSN:
- 0735-9640
- Subject:
- Arabidopsis thaliana; Nicotiana benthamiana; disease resistance; enzymes; gene silencing; genes; messenger RNA; mutants; plant pathogens; ubiquitin
- Abstract:
- ... One of the three main components of the ubiquitylation system is an ubiquitin-conjugating enzyme (UBC, E2) that attaches ubiquitin (Ub) to a substrate. A strong link has been discovered between ubiquitylation and regulation of plant defense responses against plant pathogens. In this study, the role of UBC in response to pathogen attack is investigated in Arabidopsis thaliana as it has three Ubc ge ...
- DOI:
- 10.1007/s11105-012-0502-y
-
http://dx.doi.org/10.1007/s11105-012-0502-y
- Author:
- Feng, Jiayue; Li, Jing; Liu, Hong; Gao, Qinghua; Duan, Ke; Zou, Zhirong
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 443-456
- ISSN:
- 0735-9640
- Subject:
- nucleotides; phylogeny; signal transduction; heat treatment; roots; strawberries; open reading frames; meristems; amino acids; introns; leaves; complementary DNA; calcium; onions; sodium chloride; abiotic stress; exons; Fragaria vesca; proteins; fruits; protein kinases; flowers; abscisic acid
- Abstract:
- ... Plant calcium-dependent protein kinases (CDPKs) play vital roles in calcium signal transduction during various developmental processes and during responses to biotic and abiotic stresses. Here, we isolated and characterized a CDPK gene designated FvCDPK1 from a wild diploid strawberry accession Heilongjiang-3 (Fragaria vesca L.). The FvCDPK1 gene contains 12 exons and 11 introns, and the sequences ...
- DOI:
- 10.1007/s11105-012-0513-8
-
http://dx.doi.org/10.1007/s11105-012-0513-8
- Author:
- Socquet-Juglard, D.; Christen, D.; Devènes, G.; Gessler, C.; Duffy, B.; Patocchi, A.
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 387-397
- ISSN:
- 0735-9640
- Subject:
- quantitative trait loci; fruits; total soluble solids; linkage groups; budbreak; Prunus; fruit quality; tree and stand measurements; trees; color; branches; apricots
- Abstract:
- ... The F1 population “Harostar” × “Rouge de Mauves” was used to perform a quantitative trait loci (QTL) mapping for tree architecture traits (i.e., tree diameter, total branch number, tree shape); time to initial reproductive bud break; and fruit quality traits (i.e., ground color, fruit form, soluble solid content) using data collected from 2010 to 2012. For architectural traits, interval mapping de ...
- DOI:
- 10.1007/s11105-012-0511-x
-
http://dx.doi.org/10.1007/s11105-012-0511-x
- Author:
- Wang, Yinlei; Yang, Wencai; Zhang, Wei; Han, Qing; Feng, Miao; Shen, Huolin
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 352-362
- ISSN:
- 0735-9640
- Subject:
- Meloidogyne incognita; Solanum lycopersicum; breeding lines; chromosomes; crop production; cross resistance; disease control; dominant genes; genotype; heat stability; marker-assisted selection; molecular cloning; pest resistance; root-knot nematodes; soil temperature; tomatoes
- Abstract:
- ... Root-knot nematodes (Meloidogyne spp.) can cause severe problems in tomato production in warm climates. To date, Mi-1 is the only gene that has been used widely to develop cultivars for controlling disease caused by nematodes around the world. However, Mi-1 does not provide resistance to the pest when the soil temperature is above 28 °C. Tomato breeding line ZN17 has been reported to possess resis ...
- DOI:
- 10.1007/s11105-012-0505-8
-
http://dx.doi.org/10.1007/s11105-012-0505-8
- Author:
- Yong, Seok Yien Christina; Wickneswari, Ratnam
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 303-313
- ISSN:
- 0735-9640
- Subject:
- Acacia auriculiformis; Acacia mangium; algorithms; amino acid sequences; amino acids; cell walls; cellulose; cellulose synthase; complementary DNA; gene expression; hybrids; introns; multigene family; polymerase chain reaction; prediction; protein subunits; proteins; quantitative analysis; roots; sequence analysis; trees; wood
- Abstract:
- ... Cellulose is the major component of plant cell walls, providing mechanical strength to the structural framework of plants. In association with lignin, hemicellulose, protein and pectin, cellulose forms the strong yet flexible bio-composite tissue of wood. Wood formation is an essential biological process and is of significant importance to the cellulosic private sector industry. Cellulose synthase ...
- DOI:
- 10.1007/s11105-012-0499-2
- PubMed:
- 24415841
- PubMed Central:
- PMC3881566
-
http://dx.doi.org/10.1007/s11105-012-0499-2
- Author:
- Liu, Bo; Lowes, Frank
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 272-279
- ISSN:
- 0735-9640
- Subject:
- cultivars; denaturing gradient gel electrophoresis; genome; internal transcribed spacers; sequence analysis; tomatoes; transcription (genetics)
- Abstract:
- ... The intra-genomic variation in the internal transcribed spacer (ITS) region has led to misleading conclusions in the evolutionary analysis of plants; understanding this variation is critical for correct evolutionary analysis based on ITS sequences. To reveal the ITS variation in tomato, entire copies of ITS1 sequences within tomato species were separated using denaturing gradient gel electrophores ...
- DOI:
- 10.1007/s11105-012-0500-0
-
http://dx.doi.org/10.1007/s11105-012-0500-0
- Author:
- Yan, Dong-Hui; Xia, Xinli; Yin, Weilun
- Source:
- Plant molecular biology reporter 2013 v.31 no.2 pp. 363-370
- ISSN:
- 0735-9640
- Subject:
- transcription factors; annuals; plant development; Arabidopsis; drought tolerance; phylogeny; drought; transcription (genetics); Populus trichocarpa; polyethylene glycol; water stress; genes; woody plants; gene expression regulation
- Abstract:
- ... Some members of the nuclear transcription factor Y subunit B (NF-YB) have been newly reported to regulate a variety of functions in annual plant development and drought tolerance. The overall goal of this study was to identify and characterize the NF-YB genes in the model tree Populus trichocarpa, with the expectation of determining which orthologs in P. euphratica, naturally distributed in semiar ...
- DOI:
- 10.1007/s11105-012-0508-5
-
http://dx.doi.org/10.1007/s11105-012-0508-5