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You searched for: Journal Plant molecular biology reporter Remove constraint Journal: Plant molecular biology reporter Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.31 no.2 Remove constraint Source: 2013 v.31 no.2
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1. A Basic Helix-Loop-Helix Gene from Poplar is Regulated by a Basic Leucine-Zipper Protein and is Involved in the ABA-Dependent Signaling Pathway

Author:
He, Lin; Gao, Caiqiu; Wang, Yucheng; Wu, Yingjie; Liu, Zhihua
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 344-351
ISSN:
0735-9640
Subject:
transcription factors; osmotic stress; mutation; transcription (genetics); plant veins; genes; gene fusion; leucine zipper; abscisic acid; Populus; promoter regions; roots; leaves
Abstract:
... Basic helix-loop-helix (bHLH) transcription factors (TF) comprise a large group of proteins that are involved in many developmental and physiological processes in plants. In this study, a bHLH gene (PkbHLH2), along with its promoter, was cloned from Populus koreana Rehd. A PkbHLH2 promoter::GUS gene fusion construct was generated to investigate the expression of PkbHLH2. The results demonstrated t ...
DOI:
10.1007/s11105-012-0507-6

2. A Dual Role for the Chloroplast Small Heat Shock Protein of Chenopodium album including Protection from Both Heat and Metal Stress

Author:
Haq, Noor Ul; Raza, Sana; Luthe, Dawn S.; Heckathorn, Scott A.; Shakeel, Samina N.
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 398-408
ISSN:
0735-9640
Subject:
Chenopodium album; cadmium; gene expression; heat; heat shock proteins; heat stress; heat tolerance; photosystem II; thylakoids
Abstract:
... The chloroplast small heat shock proteins (Cp-sHSP) protect photosystem II and thylakoid membranes during heat and other types of stresses. The amount of Cp-sHSPs produced is correlated with plant thermotolerance. Cp-sHSPs of Chenopodium album have already been shown to be heat-regulated and have a novel promoter architecture containing some conserved regulatory motifs Shakeel et al. (Plant Physio ...
DOI:
10.1007/s11105-012-0516-5

3. Analysis of B-Class Genes NAP3L3 and NAP3L4 in Narcissus tazetta var. chinensis

Author:
Li, Xiao-Fang; Xu, Jing; Yang, Ru; Jia, Lin-Yan; Deng, Xin-Jie; Xiong, Li-Jun; Zhang, Xue-Ping; Fang, Qi; Zhang, Wei; Sun, Yue; Xu, Ling
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 255-263
ISSN:
0735-9640
Subject:
Arabidopsis; Narcissus tazetta; calyx; clones; genes; ovules; phenotype; stamens; transgenic plants; yeasts
Abstract:
... Very few flower organ identity genes have been characterized in Chinese narcissus (Narcissus tazetta var. chinensis), which has petaloid sepals. Here, we report the cloning of two full-length B-class genes, namely NAP3L3 and NAP3L4, that are orthologs of the DEFICIENS lineage. Both genes are highly expressed in the second whorl of the perianth and in the stamens. NAP3L4 is also expressed strongly ...
DOI:
10.1007/s11105-012-0493-8

4. Analysis of the Genetic Structure of a Barley Collection Using DNA Diversity Array Technology (DArT)

Author:
Ovesná, J.; Kučera, L.; Vaculová, K.; Milotová, J.; Snape, J.; Wenzl, P.; Huttner, E.; Kilian, A.; Martelli, G.; Milella, L.
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 280-288
ISSN:
0735-9640
Subject:
DNA; alleles; barley; cultivars; field experimentation; gene frequency; genetic background; genetic markers; genetic variation; malting barley; principal component analysis
Abstract:
... Analysis of the extent of genetic variation within genetic resources is important for diversity preservation and also for breeders who exploit it. We investigated the recently introduced molecular marker technique of DNA diversity array technology (DArT), with the objective of characterising diversity in the likely relatively narrow genetic background of Czech malting barley cultivars. A total of ...
DOI:
10.1007/s11105-012-0491-x

5. Characterization of a Hypersensitive Response-Induced Gene TaHIR3 from Wheat Leaves Infected with Leaf Rust

Author:
Yu, Xiu-Mei; Zhao, Wei-Quan; Yang, Wen-Xiang; Liu, Fen; Chen, Jia-Ping; Goyer, Claudia; Liu, Da-Qun
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 314-322
ISSN:
0735-9640
Subject:
barley; seeds; stems; open reading frames; polyclonal antibodies; recombinant proteins; DNA; corn; wheat; Western blotting; gene expression; leaf rust; pathogens; DNA fingerprinting; Puccinia recondita; exons; roots; leaves
Abstract:
... Hypersensitive-induced reaction (HIR) proteins and more specific members of the proliferation, ion and death superfamily participate in response to pathogen attacks and development of spontaneous hypersensitive lesions in maize and barley leaves. In the present study, a full-length TaHIR3 (1,246-bp) was cloned and characterized from wheat near-isogenic line Thatcher-Lr15 infected by Puccinia triti ...
DOI:
10.1007/s11105-012-0504-9

6. Discrimination of American Cranberry Cultivars and Assessment of Clonal Heterogeneity Using Microsatellite Markers

Author:
Fanjardo, D.; Morales, J.; Zhu, H.; Steffan, S.; Harbur, R.; Bassil, N.; Hummer, K.; Polashock, J.; Vorsa, N.; Zalapa, J.
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 264
ISSN:
0735-9640
Subject:
Vaccinium macrocarpon; alleles; cluster analysis; cranberries; cultivars; environmental factors; fruit crops; genetic heterogeneity; genetic markers; hybrids; indigenous species; microsatellite repeats; parents; progeny
Abstract:
... Cranberries (Vaccinium macrocarpon Ait.) are an economically important fruit crop derived from a North American native species. We report the application of 12 simple sequence repeats (SSR) or microsatellite markers to assess the genetic diversity of cranberry cultivars. We studied 164 samples of 21 different cranberry cultivars, 11 experimental hybrids, and 6 representative accessions of wild spe ...
Handle:
10113/55930
DOI:
10.1007/s11105-012-0497-4

7. Dynamic Regulation of Novel and Conserved miRNAs Across Various Tissues of Diverse Cucurbit Species

Author:
Manohar, Sumanth; Jagadeeswaran, Guru; Nimmakayala, Padma; Tomason, Yan; Almeida, Aldo; Sunkar, Ramanjulu; Levi, Amnon; Reddy, Umesh K.
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 335-343
ISSN:
0735-9640
Subject:
Cucurbita moschata; Cucurbita pepo; Luffa acutangula; Momordica charantia; RNA libraries; fruits; genes; leaves; microRNA; pumpkins; squashes; stems
Abstract:
... MicroRNA genes (miRNAs) encoding small non-coding RNAs are abundant in plant genomes and play a key role in regulating several biological mechanisms. Five conserved miRNAs, miR156, miR168-1, miR168-2, miR164, and miR166 were selected for analysis from the 21 known plant miRNA families that were recovered from deep sequencing data of small RNA libraries of pumpkin and squash. A total of six novel m ...
DOI:
10.1007/s11105-012-0506-7

8. Expression Analysis of Segmentally Duplicated ZmMPK3-1 and ZmMPK3-2 genes in Maize

Author:
Liu, Yukun; Wang, Li; Zhang, Dan; Li, Dequan
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 457-463
ISSN:
0735-9640
Subject:
abscisic acid; alternative splicing; corn; gene duplication; genes; leaves; mitogen-activated protein kinase; roots; sodium chloride; stems
Abstract:
... Gene duplication and alternative splicing (AS) are two evolutionary mechanisms that can increase functional diversification of genes. Here, we found that a previously uncharacterized ZmMPK4 (ZmMPK3-1b in this research) is a splicing variant. ZmMPK3-1 can undergo AS by retaining the third intron (90 nucleotides) to generate an atypical mitogen-activated protein kinase (MAPK) gene: ZmMPK3-1b. Furthe ...
DOI:
10.1007/s11105-012-0489-4

9. Expression of the MSI-99m Gene in Transgenic Potato Plants Confers Resistance to Phytophthora infestans and Ralstonia solanacearum

Author:
Hong, Yong-bo; Liu, Shui-ping; Zhu, Yan-ping; Xie, Chao; Jue, Deng-wei; Chen, Min; Kaleri, Hubdar Ali; Yang, Qing
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 418-424
ISSN:
0735-9640
Subject:
Phytophthora infestans; Ralstonia solanacearum; Southern blotting; Xenopus laevis; antimicrobial peptides; disease resistance; frogs; pathogens; polymerase chain reaction; potatoes; secretion; synthetic genes; transgenic plants
Abstract:
... Magainins are a class of antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. MSI-99 is a synthesized magainin II analogue with high inhibitory effects to a wide spectrum of microbial organisms, including bacteria, fungi and viruses. To verify the resistance conferred by the MSI-99m gene (a MSI-99 synthetic gene with codon usage adapted for expression in ...
DOI:
10.1007/s11105-012-0503-x

10. Flow Karyotyping of Wheat Addition Line “T240” with a Haynaldia villosa 6VS Telosome

Author:
Guo, Dong-wei; Min, Dong-hong; Xu, Zhao-shi; Chen, Ming; Li, Lian-cheng; Ashraf, Muhammad; Ghafoor, Abdul; Ma, You-zhi
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 289-295
ISSN:
0735-9640
Subject:
DNA; DNA libraries; Dasypyrum villosum; chromosome addition; chromosomes; fluorescence in situ hybridization; homogenizers; karyotyping; metaphase; root tips; trifluralin; wheat
Abstract:
... To construct a chromosome-specific DNA library of chromosome 6VS from Haynaldia villosa, a wheat alien chromosome addition line T240 with a 6VS chromosome arm and its parental common wheat cv. CA921 were used to optimize protocols for preparing chromosome suspension amenable to flow sorting of the 6VS chromosome. Our results showed that root tips incubated sequentially in Hogland’s solution contai ...
DOI:
10.1007/s11105-012-0492-9

11. Gene Expression of Lupeol Synthase and Biosynthesis of Nitric Oxide in Cell Suspension Cultures of Betula platyphylla in Response to a Phomopsis Elicitor

Author:
Fan, Guizhi; Zhai, Qiaoli; Zhan, Yaguang
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 296-302
ISSN:
0735-9640
Subject:
biosynthesis; sodium azide; nitric oxide; Betula pendula subsp. mandshurica; nitrate reductase; biochemical pathways; gene expression; esters; cell suspension culture; Diaporthe
Abstract:
... The current work aimed to characterize the generation of nitric oxide (NO) and gene expression of lupeol synthase (LUS) in Betula platyphylla cells exposed to a Phomopsis elicitor. The effects of nitrate reductase (NR) and NO synthase (NOS), the two key enzymes responsible for endogenous NO biosynthesis in plants, were also investigated. NO production in B. platyphylla cell cultures exhibited a bi ...
DOI:
10.1007/s11105-012-0501-z

12. Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)

Author:
Li, Jinhua; Sima, Wei; Ouyang, Bo; Luo, Zhidan; Yang, Changxian; Ye, Zhibiao; Li, Hanxia
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 409-417
ISSN:
0735-9640
Subject:
apical meristems; tomatoes; messenger RNA; transcription factors; transcriptional activation; Solanum pennellii; stress response; gel electrophoresis; yeasts; amino acid sequences; heat stress; genes; abscisic acid; cell cycle; zinc finger motif
Abstract:
... The mammalian zinc finger protein ZPR1 is known to be essential in cell cycle progression, but its homolog in plants remains to be identified. Here, we isolated SpZPR1 from the wild tomato species Solanum pennellii LA0716 and found that its protein sequence shares homology with ZPR1 genes from other organisms. SpZPR1 mRNA was found to accumulate in tomato apical meristem in response to ABA (abscis ...
DOI:
10.1007/s11105-012-0509-4

13. Identification of a Coding Sequence and Structure Modeling of a Glycine-Rich RNA-Binding Protein (CmGRP1) from Chelidonium majus L.

Author:
Nawrot, Robert; Tomaszewski, Łukasz; Czerwoniec, Anna; Goździcka-Józefiak, Anna
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 470-476
ISSN:
0735-9640
Subject:
Chelidonium majus; DNA; DNA primers; RNA; RNA-binding proteins; amino acids; cell wall components; crystal structure; models; nucleotide sequences; plant proteins; polymerase chain reaction; protein-protein interactions; sequence analysis
Abstract:
... The family of glycine-rich plant proteins (GRPs) is a large and complex group of proteins that share, as a common feature, the presence of glycine-rich domains arranged in (Gly)n-X repeats that are suggested to be involved in protein–protein interactions, RNA binding, and nucleolar targeting. These proteins are implicated in several independent physiological processes. Some are components of cell ...
DOI:
10.1007/s11105-012-0510-y
PubMed:
24415842
PubMed Central:
PMC3881573

14. In Planta Evidence for the Involvement of a Ubiquitin Conjugating Enzyme (UBC E2 clade) in Negative Regulation of Disease Resistance

Author:
Unver, Turgay; Turktas, Mine; Budak, Hikmet
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 323-334
ISSN:
0735-9640
Subject:
Arabidopsis thaliana; Nicotiana benthamiana; disease resistance; enzymes; gene silencing; genes; messenger RNA; mutants; plant pathogens; ubiquitin
Abstract:
... One of the three main components of the ubiquitylation system is an ubiquitin-conjugating enzyme (UBC, E2) that attaches ubiquitin (Ub) to a substrate. A strong link has been discovered between ubiquitylation and regulation of plant defense responses against plant pathogens. In this study, the role of UBC in response to pathogen attack is investigated in Arabidopsis thaliana as it has three Ubc ge ...
DOI:
10.1007/s11105-012-0502-y

15. Isolation and Characterization of a Calcium-Dependent Protein Kinase Gene, FvCDPK1, Responsive to Abiotic Stress in Woodland Strawberry (Fragaria vesca)

Author:
Feng, Jiayue; Li, Jing; Liu, Hong; Gao, Qinghua; Duan, Ke; Zou, Zhirong
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 443-456
ISSN:
0735-9640
Subject:
nucleotides; phylogeny; signal transduction; heat treatment; roots; strawberries; open reading frames; meristems; amino acids; introns; leaves; complementary DNA; calcium; onions; sodium chloride; abiotic stress; exons; Fragaria vesca; proteins; fruits; protein kinases; flowers; abscisic acid
Abstract:
... Plant calcium-dependent protein kinases (CDPKs) play vital roles in calcium signal transduction during various developmental processes and during responses to biotic and abiotic stresses. Here, we isolated and characterized a CDPK gene designated FvCDPK1 from a wild diploid strawberry accession Heilongjiang-3 (Fragaria vesca L.). The FvCDPK1 gene contains 12 exons and 11 introns, and the sequences ...
DOI:
10.1007/s11105-012-0513-8

16. Mapping Architectural, Phenological, and Fruit Quality QTLs in Apricot

Author:
Socquet-Juglard, D.; Christen, D.; Devènes, G.; Gessler, C.; Duffy, B.; Patocchi, A.
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 387-397
ISSN:
0735-9640
Subject:
quantitative trait loci; fruits; total soluble solids; linkage groups; budbreak; Prunus; fruit quality; tree and stand measurements; trees; color; branches; apricots
Abstract:
... The F1 population “Harostar” × “Rouge de Mauves” was used to perform a quantitative trait loci (QTL) mapping for tree architecture traits (i.e., tree diameter, total branch number, tree shape); time to initial reproductive bud break; and fruit quality traits (i.e., ground color, fruit form, soluble solid content) using data collected from 2010 to 2012. For architectural traits, interval mapping de ...
DOI:
10.1007/s11105-012-0511-x

17. Mapping of a Heat-Stable Gene for Resistance to Southern Root-Knot Nematode in Solanum lycopersicum

Author:
Wang, Yinlei; Yang, Wencai; Zhang, Wei; Han, Qing; Feng, Miao; Shen, Huolin
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 352-362
ISSN:
0735-9640
Subject:
Meloidogyne incognita; Solanum lycopersicum; breeding lines; chromosomes; crop production; cross resistance; disease control; dominant genes; genotype; heat stability; marker-assisted selection; molecular cloning; pest resistance; root-knot nematodes; soil temperature; tomatoes
Abstract:
... Root-knot nematodes (Meloidogyne spp.) can cause severe problems in tomato production in warm climates. To date, Mi-1 is the only gene that has been used widely to develop cultivars for controlling disease caused by nematodes around the world. However, Mi-1 does not provide resistance to the pest when the soil temperature is above 28 °C. Tomato breeding line ZN17 has been reported to possess resis ...
DOI:
10.1007/s11105-012-0505-8

18. Molecular characterization of a cellulose synthase gene (AaxmCesA1) isolated from an Acacia auriculiformis x Acacia mangium hybrid

Author:
Yong, Seok Yien Christina; Wickneswari, Ratnam
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 303-313
ISSN:
0735-9640
Subject:
Acacia auriculiformis; Acacia mangium; algorithms; amino acid sequences; amino acids; cell walls; cellulose; cellulose synthase; complementary DNA; gene expression; hybrids; introns; multigene family; polymerase chain reaction; prediction; protein subunits; proteins; quantitative analysis; roots; sequence analysis; trees; wood
Abstract:
... Cellulose is the major component of plant cell walls, providing mechanical strength to the structural framework of plants. In association with lignin, hemicellulose, protein and pectin, cellulose forms the strong yet flexible bio-composite tissue of wood. Wood formation is an essential biological process and is of significant importance to the cellulosic private sector industry. Cellulose synthase ...
DOI:
10.1007/s11105-012-0499-2
PubMed:
24415841
PubMed Central:
PMC3881566

19. Multiple Divergent ITS1 Copies Were Identified in Single Tomato Genome Using DGGE Analysis

Author:
Liu, Bo; Lowes, Frank
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 272-279
ISSN:
0735-9640
Subject:
cultivars; denaturing gradient gel electrophoresis; genome; internal transcribed spacers; sequence analysis; tomatoes; transcription (genetics)
Abstract:
... The intra-genomic variation in the internal transcribed spacer (ITS) region has led to misleading conclusions in the evolutionary analysis of plants; understanding this variation is critical for correct evolutionary analysis based on ITS sequences. To reveal the ITS variation in tomato, entire copies of ITS1 sequences within tomato species were separated using denaturing gradient gel electrophores ...
DOI:
10.1007/s11105-012-0500-0

20. NF-YB Family Genes Identified in a Poplar Genome-wide Analysis and Expressed in Populus euphratica Are Responsive to Drought Stress

Author:
Yan, Dong-Hui; Xia, Xinli; Yin, Weilun
Source:
Plant molecular biology reporter 2013 v.31 no.2 pp. 363-370
ISSN:
0735-9640
Subject:
transcription factors; annuals; plant development; Arabidopsis; drought tolerance; phylogeny; drought; transcription (genetics); Populus trichocarpa; polyethylene glycol; water stress; genes; woody plants; gene expression regulation
Abstract:
... Some members of the nuclear transcription factor Y subunit B (NF-YB) have been newly reported to regulate a variety of functions in annual plant development and drought tolerance. The overall goal of this study was to identify and characterize the NF-YB genes in the model tree Populus trichocarpa, with the expectation of determining which orthologs in P. euphratica, naturally distributed in semiar ...
DOI:
10.1007/s11105-012-0508-5