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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 2010 Remove constraint Publication Year: 2010 Source 2010 v.154 no.2 Remove constraint Source: 2010 v.154 no.2
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1. Abscisic Acid Deficiency Causes Changes in Cuticle Permeability and Pectin Composition That Influence Tomato Resistance to Botrytis cinerea

Author:
Curvers, Katrien; Seifi, Hamed; Mouille, Grégory; de Rycke, Riet; Asselbergh, Bob; Van Hecke, Annelies; Vanderschaeghe, Dieter; Höfte, Herman; Callewaert, Nico; Van Breusegem, Frank; Höfte, Monica
Source:
Plant physiology 2010 v.154 no.2 pp. 847-860
ISSN:
0032-0889
Subject:
Botrytis cinerea; Solanum lycopersicum; abscisic acid; cell walls; correlation; disease resistance; fungi; gene expression; mutants; oligosaccharides; pectins; permeability; physicochemical properties; tomatoes
Abstract:
... A mutant of tomato (Solanum lycopersicum) with reduced abscisic acid (ABA) production (sitiens) exhibits increased resistance to the necrotrophic fungus Botrytis cinerea. This resistance is correlated with a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that is absent in tomato with normal ABA production. Moreover, basal expression of defense genes i ...
DOI:
10.1104/pp.110.158972
PubMed:
20709830
PubMed Central:
PMC2949027

2. A Gain-of-Function Mutation in the Arabidopsis Disease Resistance Gene RPP4 Confers Sensitivity to Low Temperature

Author:
Huang, Xiaozhen; Li, Jianyong; Bao, Fei; Zhang, Xiaoyan; Yang, Shuhua
Source:
Plant physiology 2010 v.154 no.2 pp. 796-809
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Peronospora parasitica; alleles; amino acid substitution; cell death; clones; disease resistance; genetic techniques and protocols; hydrogen peroxide; messenger RNA; mutants; phenotype; plant growth; proteins; salicylic acid; temperature
Abstract:
... How plants adapt to low temperature is not well understood. To identify components involved in low-temperature signaling, we characterized the previously isolated chilling-sensitive2 mutant (chs2) of Arabidopsis (Arabidopsis thaliana). This mutant grew normally at 22°C but showed phenotypes similar to activation of defense responses when shifted to temperatures below 16°C. These phenotypes include ...
DOI:
10.1104/pp.110.157610
PubMed:
20699401
PubMed Central:
PMC2949010

3. Analysis of the Rice Mitochondrial Carrier Family Reveals Anaerobic Accumulation of a Basic Amino Acid Carrier Involved in Arginine Metabolism during Seed Germination

Author:
Taylor, Nicolas L.; Howell, Katharine A.; Heazlewood, Joshua L.; Tan, Tzu Yien W.; Narsai, Reena; Huang, Shaobai; Whelan, James; Millar, A. Harvey
Source:
Plant physiology 2010 v.154 no.2 pp. 691-704
ISSN:
0032-0889
Subject:
rice; Oryza sativa; ornithine; genes; mass spectrometry; gel electrophoresis; transport proteins; coleoptiles; proteome; aerobic conditions; gene expression; sodium; arginase; anaerobic conditions; metabolism; lung function; arginine; seed germination; urea; nitrates
Abstract:
... Given the substantial changes in mitochondrial gene expression, the mitochondrial proteome, and respiratory function during rice (Oryza sativa) germination under anaerobic and aerobic conditions, we have attempted to identify changes in mitochondrial membrane transport capacity during these processes. We have assembled a preliminary rice mitochondrial carrier gene family of 50 members, defined its ...
DOI:
10.1104/pp.110.162214
PubMed:
20720170
PubMed Central:
PMC2948988

4. Antisense Down-Regulation of 4CL Expression Alters Lignification, Tree Growth, and Saccharification Potential of Field-Grown Poplar

Author:
Voelker, Steven L.; Lachenbruch, Barbara; Meinzer, Frederick C.; Jourdes, Michael; Ki, Chanyoung; Patten, Ann M.; Davin, Laurence B.; Lewis, Norman G.; Tuskan, Gerald A.; Gunter, Lee; Decker, Stephen R.; Selig, Michael J.; Sykes, Robert; Himmel, Michael E.; Kitin, Peter; Shevchenko, Olga; Strauss, Steven H.
Source:
Plant physiology 2010 v.154 no.2 pp. 874-886
ISSN:
0032-0889
Subject:
Populus alba; Populus tremula; RNA; cell walls; ethanol; ethanol production; genes; lignification; lignin; metabolism; paper; plant biochemistry; saccharification; tree growth; trees; wood; wood chemistry
Abstract:
... Transgenic down-regulation of the Pt4CL1 gene family encoding 4-coumarate:coenzyme A ligase (4CL) has been reported as a means for reducing lignin content in cell walls and increasing overall growth rates, thereby improving feedstock quality for paper and bioethanol production. Using hybrid poplar (Populus tremula x Populus alba), we applied this strategy and examined field-grown transformants for ...
DOI:
10.1104/pp.110.159269
PubMed:
20729393
PubMed Central:
PMC2949011

5. A Suite of Lotus japonicus Starch Mutants Reveals Both Conserved and Novel Features of Starch Metabolism

Author:
Vriet, Cécile; Welham, Tracey; Brachmann, Andreas; Pike, Marilyn; Pike, Jodie; Perry, Jillian; Parniske, Martin; Sato, Shusei; Tabata, Satoshi; Smith, Alison M.; Wang, Trevor L.
Source:
Plant physiology 2010 v.154 no.2 pp. 643-655
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Lotus corniculatus var. japonicus; carbon; genes; leaves; legumes; metabolism; mutants; nitrogen fixation; phenotype; phosphoglucomutase; photoperiod; plant fertility; regrowth; seed development; starch; structure-activity relationships
Abstract:
... The metabolism of starch is of central importance for many aspects of plant growth and development. Information on leaf starch metabolism other than in Arabidopsis (Arabidopsis thaliana) is scarce. Furthermore, its importance in several agronomically important traits exemplified by legumes remains to be investigated. To address this issue, we have provided detailed information on the genes involve ...
DOI:
10.1104/pp.110.161844
PubMed:
20699404
PubMed Central:
PMC2949007

6. ATP-Binding Cassette Transporter G26 Is Required for Male Fertility and Pollen Exine Formation in Arabidopsis

Author:
Quilichini, Teagen D.; Friedmann, Michael C.; Samuels, A. Lacey; Douglas, Carl J.
Source:
Plant physiology 2010 v.154 no.2 pp. 678-690
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; exine; messenger RNA; biosynthesis; fruits; seeds; biopolymers; polymerization; spores; models; selfing; pollen; microspores; transmission electron microscopy; mutants; plasma membrane; epicuticular wax; endoplasmic reticulum; ABC transporters
Abstract:
... The highly resistant biopolymer, sporopollenin, gives the outer wall (exine) of spores and pollen grains their unparalleled strength, shielding these structures from terrestrial stresses. Despite a limited understanding of the composition of sporopollenin, it appears that the synthesis of sporopollenin occurs in the tapetum and requires the transport of one or more sporopollenin constituents to th ...
DOI:
10.1104/pp.110.161968
PubMed:
20732973
PubMed Central:
PMC2949020

7. Characterization of a Developmental Root Response Caused by External Ammonium Supply in Lotus japonicus

Author:
Rogato, Alessandra; D'Apuzzo, Enrica; Barbulova, Ani; Omrane, Selim; Parlati, Aurora; Carfagna, Simona; Costa, Alex; Schiavo, Fiorella Lo; Esposito, Sergio; Chiurazzi, Maurizio
Source:
Plant physiology 2010 v.154 no.2 pp. 784-795
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant development; nutritional status; phenotype; root systems; soil nutrients; nitrogen; topology; transporters; genes; toxicity; biomass; Lotus corniculatus var. japonicus; roots; coping strategies; nitrates; shoots
Abstract:
... Plants respond to changes of nutrient availability in the soil by modulating their root system developmental plan. This response is mediated by systemic changes of the nutritional status and/or by local perception of specific signals. The effect of nitrate on Arabidopsis (Arabidopsis thaliana) root development represents a paradigm of these responses, and nitrate transporters are involved both in ...
DOI:
10.1104/pp.110.160309
PubMed:
20688979
PubMed Central:
PMC2948985

8. Coexpression Analysis Identifies Rice Starch Regulator1, a Rice AP2/EREBP Family Transcription Factor, as a Novel Rice Starch Biosynthesis Regulator

Author:
Fu, Fang-Fang; Xue, Hong-Wei
Source:
Plant physiology 2010 v.154 no.2 pp. 927-938
ISSN:
0032-0889
Subject:
Oryza sativa; amylopectin; amylose; binding proteins; biochemical pathways; biosynthesis; crop quality; gelatinization; genes; knockout mutants; nutritive value; physicochemical properties; plant development; rice; rice starch; seed yield; seeds; starch granules; temperature; transcription factors
Abstract:
... Starch biosynthesis is important for plant development and is a critical factor in crop quality and nutrition. As a complex metabolic pathway, the regulation of starch biosynthesis is still poorly understood. We here present the identification of candidate regulators for starch biosynthesis by gene coexpression analysis in rice (Oryza sativa). Starch synthesis genes can be grouped into type I (in ...
DOI:
10.1104/pp.110.159517
PubMed:
20713616
PubMed Central:
PMC2949045

9. ¹³C-Tracer and Gas Chromatography-Mass Spectrometry Analyses Reveal Metabolic Flux Distribution in the Oleaginous Microalga Chlorella protothecoides

Author:
Xiong, Wei; Liu, Lixia; Wu, Chao; Yang, Chen; Wu, Qingyu
Source:
Plant physiology 2010 v.154 no.2 pp. 1001-1011
ISSN:
0032-0889
Subject:
Chlorella; NADP (coenzyme); amino acids; biodiesel; biosynthesis; carbon; engineering; enzyme activity; feedstocks; gas chromatography; glucose; glycolysis; glyoxylate cycle; labeling techniques; lipid content; malic enzyme; mass spectrometry; microalgae; nitrogen; pentoses; phosphates; phosphoenolpyruvate carboxylase; topology; triacylglycerols; tricarboxylic acid cycle
Abstract:
... The green alga Chlorella protothecoides has received considerable attention because it accumulates neutral triacylglycerols, commonly regarded as an ideal feedstock for biodiesel production. In order to gain a better understanding of its metabolism, tracer experiments with [U-¹³C]/[1-¹³C]glucose were performed with heterotrophic growth of C. protothecoides for identifying the metabolic network top ...
DOI:
10.1104/pp.110.158956
PubMed:
20720172
PubMed Central:
PMC2948989

10. Endogenous Abscisic Acid as a Key Switch for Natural Variation in Flooding-Induced Shoot Elongation

Author:
Chen, Xin; Pierik, Ronald; Peeters, Anton J.M.; Poorter, Hendrik; Visser, Eric J.W.; Huber, Heidrun; de Kroon, Hans; Voesenek, Laurentius A.C.J.
Source:
Plant physiology 2010 v.154 no.2 pp. 969-977
ISSN:
0032-0889
Subject:
Rumex acetosa; Rumex palustris; abscisic acid; carbohydrates; ethylene; floodplains; floods; gibberellic acid; lakes; natural selection; petioles; rivers; shoots; signal transduction; stem elongation; submergence; wetland plants
Abstract:
... Elongation of leaves and stem is a key trait for survival of terrestrial plants during shallow but prolonged floods that completely submerge the shoot. However, natural floods at different locations vary strongly in duration and depth, and, therefore, populations from these locations are subjected to different selection pressure, leading to intraspecific variation. Here, we identified the signal t ...
DOI:
10.1104/pp.110.162792
PubMed:
20699400
PubMed Central:
PMC2949041

11. Evidence That High Activity of Vacuolar Invertase Is Required for Cotton Fiber and Arabidopsis Root Elongation through Osmotic Dependent and Independent Pathways, Respectively

Author:
Wang, Lu; Li, Xiao-Rong; Lian, Heng; Ni, Di-An; He, Yu-ke; Chen, Xiao-Ya; Ruan, Yong-Ling
Source:
Plant physiology 2010 v.154 no.2 pp. 744-756
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Gossypium; RNA interference; beta-fructofuranosidase; cell growth; cell walls; genotype; leaves; lint cotton; mutants; osmolality; phenotype; plant tissues; root growth; roots; seed cotton; seeds; stems; sugars; transfer DNA; trichomes; vacuoles
Abstract:
... Vacuolar invertase (VIN) has long been considered as a major player in cell expansion. However, direct evidence for this view is lacking due, in part, to the complexity of multicellular plant tissues. Here, we used cotton (Gossypium spp.) fibers, fast-growing single-celled seed trichomes, to address this issue. VIN activity in elongating fibers was approximately 4-6-fold higher than that in leaves ...
DOI:
10.1104/pp.110.162487
PubMed:
20699399
PubMed Central:
PMC2948991

12. Extracellular Nucleotides Elicit Cytosolic Free Calcium Oscillations in Arabidopsis

Author:
Tanaka, Kiwamu; Swanson, Sarah J.; Gilroy, Simon; Stacey, Gary
Source:
Plant physiology 2010 v.154 no.2 pp. 705-719
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; G-proteins; adenosine triphosphate; antagonists; calcium; energy; fluorescence; guanosine triphosphate; mutants; plant response; receptors; roots; secretion
Abstract:
... Extracellular ATP induces a rise in the level of cytosolic free calcium ([Ca²⁺]cyt) in plant cells. To expand our knowledge about the function of extracellular nucleotides in plants, the effects of several nucleotide analogs and pharmacological agents on [Ca²⁺]cyt changes were studied using transgenic Arabidopsis (Arabidopsis thaliana) expressing aequorin or the fluorescence resonance energy trans ...
DOI:
10.1104/pp.110.162503
PubMed:
20671112
PubMed Central:
PMC2949047

13. How Do We Improve Crop Production in a Warming World?

Author:
Ainsworth, Elizabeth A.; Ort, Donald R.
Source:
Plant physiology 2010 v.154 no.2 pp. 526
ISSN:
0032-0889
Subject:
crop production; crop yield; plant cultural practices; photosynthesis; global warming
Abstract:
... This paper identifies the regional priorities and biological targets for adaptation of agriculture to rising temperature and highlights the possibility of integrating new technologies with conventional selection-based breeding to improve crop production under global warming. ...
Handle:
10113/49243
DOI:
10.1104/pp.110.161349
PubMed:
20921178
PubMed Central:
PMC2949002

14. Identification and Characterization of in Vitro Galactosyltransferase Activities Involved in Arabinogalactan-Protein Glycosylation in Tobacco and Arabidopsis

Author:
Liang, Yan; Faik, Ahmed; Kieliszewski, Marcia; Tan, Li; Xu, Wen-Liang; Showalter, Allan M.
Source:
Plant physiology 2010 v.154 no.2 pp. 632-642
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Nicotiana tabacum; biosynthesis; centrifugation; fractionation; genes; glycoproteins; glycosylation; high performance liquid chromatography; hydrolysates; hydroxyproline; in vitro studies; mass spectrometry; monosaccharides; sucrose; tobacco; uridine diphosphate
Abstract:
... Arabinogalactan-proteins (AGPs) are highly glycosylated hydroxyproline (Hyp)-rich glycoproteins that are frequently characterized by the presence of [Alanine-Hyp] ([AO]) repetitive units. AGP galactosyltransferase (GalT) activities in tobacco (Nicotiana tabacum) and Arabidopsis (Arabidopsis thaliana) microsomal membranes were studied here with an in vitro GalT reaction system, which used acceptor ...
DOI:
10.1104/pp.110.160051
PubMed:
20671109
PubMed Central:
PMC2949012

15. Increased Activity of the Vacuolar Monosaccharide Transporter TMT1 Alters Cellular Sugar Partitioning, Sugar Signaling, and Seed Yield in Arabidopsis

Author:
Wingenter, Karina; Schulz, Alexander; Wormit, Alexandra; Wic, Stefan; Trentmann, Oliver; Hoermiller, Imke I.; Heyer, Arnd G.; Marten, Irene; Hedrich, Rainer; Neuhaus, H. Ekkehard
Source:
Plant physiology 2010 v.154 no.2 pp. 665-677
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; biomass; critical load; early development; fractionation; gene expression; genes; leaves; lipid content; messenger RNA; monosaccharides; mutants; nitrates; seed yield; seeds; soil; sucrose; transporters; vacuoles
Abstract:
... The extent to which vacuolar sugar transport activity affects molecular, cellular, and developmental processes in Arabidopsis (Arabidopsis thaliana) is unknown. Electrophysiological analysis revealed that overexpression of the tonoplast monosaccharide transporter TMT1 in a tmt1-2::tDNA mutant led to increased proton-coupled monosaccharide import into isolated mesophyll vacuoles in comparison with ...
DOI:
10.1104/pp.110.162040
PubMed:
20709831
PubMed Central:
PMC2949046

16. Leaf Senescence Signaling: The Ca²⁺-Conducting Arabidopsis Cyclic Nucleotide Gated Channel2 Acts through Nitric Oxide to Repress Senescence Programming

Author:
Ma, Wei; Smigel, Andries; Walker, Robin K.; Moeder, Wolfgang; Yoshioka, Keiko; Berkowitz, Gerald A.
Source:
Plant physiology 2010 v.154 no.2 pp. 733-743
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant development; phenotype; nitric oxide; lipid peroxidation; plant pathogens; gene expression; genes; mutants; salicylic acid; cyclic nucleotides; chlorophyll; genetic relationships; leaves
Abstract:
... Ca²⁺ and nitric oxide (NO) are essential components involved in plant senescence signaling cascades. In other signaling pathways, NO generation can be dependent on cytosolic Ca²⁺. The Arabidopsis (Arabidopsis thaliana) mutant dnd1 lacks a plasma membrane-localized cation channel (CNGC2). We recently demonstrated that this channel affects plant response to pathogens through a signaling cascade invo ...
DOI:
10.1104/pp.110.161356
PubMed:
20699402
PubMed Central:
PMC2949008

17. Light Quality-Mediated Petiole Elongation in Arabidopsis during Shade Avoidance Involves Cell Wall Modification by Xyloglucan Endotransglucosylase/Hydrolases

Author:
Sasidharan, Rashmi; Chinnappa, C.C.; Staal, Marten; Elzenga, J. Theo M.; Yokoyama, Ryusuke; Nishitani, Kazuhiko; Voesenek, Laurentius A.C.J.; Pierik, Ronald
Source:
Plant physiology 2010 v.154 no.2 pp. 978-990
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; acidification; apoplast; canopy; cell walls; genes; hydrolases; knockout mutants; pH; petioles; proteins; protons; red light; shoots; white light; xyloglucan:xyloglucosyl transferase
Abstract:
... Some plants can avoid shaded conditions via rapid shoot elongation, thus growing into better lit areas in a canopy. Cell wall-modifying mechanisms promoting this elongation response, therefore, are important regulatory points during shade avoidance. Two major cell wall-modifying protein families are expansins and xyloglucan endotransglucosylase/hydrolases (XTHs). The role of these proteins during ...
DOI:
10.1104/pp.110.162057
PubMed:
20688978
PubMed Central:
PMC2949003

18. Nitric Oxide Acts Downstream of Auxin to Trigger Root Ferric-Chelate Reductase Activity in Response to Iron Deficiency in Arabidopsis

Author:
Chen, Wei Wei; Yang, Jian Li; Qin, Cheng; Jin, Chong Wei; Mo, Ji Hao; Ye, Ting; Zheng, Shao Jian
Source:
Plant physiology 2010 v.154 no.2 pp. 810-819
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; acids; auxins; basic helix-loop-helix transcription factors; esters; gene expression; genes; mutants; nitric oxide; plasma membrane; roots
Abstract:
... In response to iron (Fe) deficiency, dicots employ a reduction-based mechanism by inducing ferric-chelate reductase (FCR) at the root plasma membrane to enhance Fe uptake. However, the signal pathway leading to FCR induction is still unclear. Here, we found that the Fe-deficiency-induced increase of auxin and nitric oxide (NO) levels in wild-type Arabidopsis (Arabidopsis thaliana) was accompanied ...
DOI:
10.1104/pp.110.161109
PubMed:
20699398
PubMed Central:
PMC2948983

19. Nonreductive Iron Uptake Mechanism in the Marine Alveolate Chromera velia

Author:
Sutak, Robert; Šlapeta, Jan; San Roman, Mabel; Camadro, Jean-Michel; Lesuisse, Emmanuel
Source:
Plant physiology 2010 v.154 no.2 pp. 991-1000
ISSN:
0032-0889
Subject:
Saccharomyces cerevisiae; binding sites; cell walls; chelates; chelating agents; citrates; electron transfer; ions; iron; microalgae; models; oceans; photosynthesis; uptake mechanisms; yeasts
Abstract:
... Chromera velia is a newly cultured photosynthetic marine alveolate. This microalga has a high iron requirement for respiration and photosynthesis, although its natural environment contains less than 1 nM of this metal. We found that this organism uses a novel mechanism of iron uptake, differing from the classic reductive and siderophore-mediated iron uptake systems characterized in the model yeast ...
DOI:
10.1104/pp.110.159947
PubMed:
20724644
PubMed Central:
PMC2949016

20. Proper Levels of the Arabidopsis Cohesion Establishment Factor CTF7 Are Essential for Embryo and Megagametophyte, But Not Endosperm, Development

Author:
Jiang, Ling; Yuan, Li; Xia, Ming; Makaroff, Christopher A.
Source:
Plant physiology 2010 v.154 no.2 pp. 820-832
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; mitosis; buds; gametophytes; yeasts; transfer DNA; mutation; Saccharomyces cerevisiae; zygote; endosperm; genes; embryogenesis; pollen; cohesion
Abstract:
... CTF7 is an essential gene in yeast that is required for the formation of sister chromatid cohesion. While recent studies have provided insights into how sister chromatid cohesion is established, less is known about how specifically CTF7 facilitates the formation of cohesion, and essentially nothing is known about how sister chromatid cohesion is established in plants. In this report, we describe t ...
DOI:
10.1104/pp.110.157560
PubMed:
20671110
PubMed Central:
PMC2949036

21. Quantitative Trait Loci, Epigenetics, Sugars, and MicroRNAs: Quaternaries in Phosphate Acquisition and Use

Author:
Vance, Carroll P.
Source:
Plant physiology 2010 v.154 no.2 pp. 582
ISSN:
0032-0889
Subject:
plant nutrition; plant adaptation; acclimation; signal transduction; quantitative trait loci; epigenetics; soil nutrients; nutrient availability; phosphorus; sugars; soil-plant interactions; gene expression regulation
Abstract:
... Significant research efforts in the genomics of P stress have shown that many suites of genes regulated in a coordinated fashion are involved in plant acclimatization to P deficiency. These genomic studies, in conjunction with traditional plant breeding, have shown that P-acclimatization traits are controlled by multiple genes, most probably in quantitative trait loci (QTLs). Future development of ...
Handle:
10113/49412
DOI:
10.1104/pp.110.161067
PubMed:
20921189
PubMed Central:
PMC2949005

22. Rapid Assessment of Gene Function in the Circadian Clock Using Artificial MicroRNA in Arabidopsis Mesophyll Protoplasts

Author:
Kim, Jeongsik; Somers, David E.
Source:
Plant physiology 2010 v.154 no.2 pp. 611-621
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; bioluminescence; casein; circadian rhythm; genes; mesophyll; microRNA; phenotype; plasmids; protoplasts
Abstract:
... Rapid assessment of the effect of reduced levels of gene products is often a bottleneck in determining how to proceed with an interesting gene candidate. Additionally, gene families with closely related members can confound determination of the role of even a single one of the group. We describe here an in vivo method to rapidly determine gene function using transient expression of artificial micr ...
DOI:
10.1104/pp.110.162271
PubMed:
20709829
PubMed Central:
PMC2949038

23. Reproductive Development Modulates Gene Expression and Metabolite Levels with Possible Feedback Inhibition of Artemisinin in Artemisia annua

Author:
Arsenault, Patrick R.; Vail, Daniel; Wobbe, Kristin K.; Erickson, Karen; Weathers, Pamela J.
Source:
Plant physiology 2010 v.154 no.2 pp. 958-968
ISSN:
0032-0889
Subject:
Artemisia annua; acids; artemisinin; biosynthesis; bracts; buds; correlation; cytochrome P-450; flowering; flowers; gene expression; genes; leaves; mature plants; models; trichomes
Abstract:
... The relationship between the transition to budding and flowering in Artemisia annua and the production of the antimalarial sesquiterpene, artemisinin (AN), the dynamics of artemisinic metabolite changes, AN-related transcriptional changes, and plant and trichome developmental changes were measured. Maximum production of AN occurs during full flower stage within floral tissues, but that changes in ...
DOI:
10.1104/pp.110.162552
PubMed:
20724645
PubMed Central:
PMC2949044

24. Stacking Multiple Transgenes at a Selected Genomic Site via Repeated Recombinase-Mediated DNA Cassette Exchanges

Author:
Li, Zhongsen; Moon, Bryan P.; Xing, Aiqiu; Liu, Zhan-Bin; McCardell, Richard P.; Damude, Howard G.; Falco, S. Carl
Source:
Plant physiology 2010 v.154 no.2 pp. 622-631
ISSN:
0032-0889
Subject:
diacylglycerol acyltransferase; lipid content; lysine; Glycine max; barley; phenotype; transgenes; Hordeum vulgare; transport proteins; DNA; Corynebacterium glutamicum; Yarrowia lipolytica; soybeans; gene silencing; amino acid composition; oleic acid; stearoyl-CoA desaturase
Abstract:
... Recombinase-mediated DNA cassette exchange (RMCE) has been successfully used to insert transgenes at previously characterized genomic sites in plants. Following the same strategy, groups of transgenes can be stacked to the same site through multiple rounds of RMCE. A gene-silencing cassette, designed to simultaneously silence soybean (Glycine max) genes fatty acid ω-6 desaturase 2 (FAD2) and acyl- ...
DOI:
10.1104/pp.110.160093
PubMed:
20720171
PubMed Central:
PMC2949048

25. Suppression of the Vacuolar Invertase Gene Prevents Cold-Induced Sweetening in Potato

Author:
Bhaskar, Pudota B.; Wu, Lei; Busse, James S.; Whitty, Brett R.; Hamernik, Andy J.; Jansky, Shelley H.; Buell, C. Robin; Bethke, Paul C.; Jiang, Jiming
Source:
Plant physiology 2010 v.154 no.2 pp. 939-948
ISSN:
0032-0889
Subject:
temperature; food crops; reducing sugars; sprouting; breeding; Solanum tuberosum; biotechnology; heat treatment; gene expression; potato chips; potatoes; beta-fructofuranosidase; color; genes; carcinogens; tubers; acrylamides; cold storage
Abstract:
... Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to prevent sprouting, minimize disease losses, and supply consumers and the processing industry with high-quality tubers throughout the year. Unfortunately, cold storage triggers an accumulation of reducing sugars in tubers. High-temperature processing of these tubers re ...
DOI:
10.1104/pp.110.162545
PubMed:
20736383
PubMed Central:
PMC2948980

26. Tethering Factors Required for Cytokinesis in Arabidopsis

Author:
Thellmann, Martha; Rybak, Katarzyna; Thiele, Knut; Wanner, Gerhard; Assaad, Farhah F.
Source:
Plant physiology 2010 v.154 no.2 pp. 720-732
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; cell membranes; cell walls; cytokinesis; electron microscopy; mutants; phenotype; transport proteins
Abstract:
... At the end of the cell cycle, the nascent cross wall is laid down within a transient membrane compartment referred to as the cell plate. Tethering factors, which act by capturing vesicles and holding them in the vicinity of their target membranes, are likely to play an important role in the first stages of cell plate assembly. Factors required for cell plate biogenesis, however, remain to be ident ...
DOI:
10.1104/pp.110.154286
PubMed:
20713617
PubMed Central:
PMC2948999

27. The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia

Author:
Anderson, Jonathan P.; Lichtenzveig, Judith; Gleason, Cynthia; Oliver, Richard P.; Singh, Karam B.
Source:
Plant physiology 2010 v.154 no.2 pp. 861-873
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Medicago truncatula; Phytophthora medicaginis; Thanatephorus cucumeris; adverse effects; canola; disease resistance; ethylene; gene overexpression; genes; genetic resistance; germplasm screening; grain crops; legumes; nodulation; phenotype; plant development; plant pathogenic fungi; promoter regions; response elements; root-knot nematodes; roots; symbiosis; transcription factors
Abstract:
... The fungal necrotrophic pathogen Rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola, and legumes. Despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has been identified, suggesting that alternative strategies to improve resistance are required. In this study, we characterize moderate resistance to R. solani anas ...
DOI:
10.1104/pp.110.163949
PubMed:
20713618
PubMed Central:
PMC2949043

28. The glabra1 Mutation Affects Cuticle Formation and Plant Responses to Microbes

Author:
Xia, Ye; Yu, Keshun; Navarre, Duroy; Seebold, Kenneth; Kachroo, Aardra; Kachroo, Pradeep
Source:
Plant physiology 2010 v.154 no.2 pp. 833-846
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; fatty acids; systemic acquired resistance; jasmonic acid; genetic background; gibberellic acid; mutation; microorganisms; biochemical pathways; plant cuticle; plant pathogens; plant response; salicylic acid; stearoyl-CoA desaturase
Abstract:
... Systemic acquired resistance (SAR) is a form of defense that provides resistance against a broad spectrum of pathogens in plants. Previous work indicates a role for plastidial glycerolipid biosynthesis in SAR. Specifically, mutations in FATTY ACID DESATURASE7 (FAD7), which lead to reduced trienoic fatty acid levels and compromised plastidial lipid biosynthesis, have been associated with defective ...
DOI:
10.1104/pp.110.161646
PubMed:
20699396
PubMed Central:
PMC2949009

29. The Metabolic and Developmental Roles of Carotenoid Cleavage Dioxygenase4 from Potato

Author:
Campbell, Raymond; Ducreux, Laurence J.M.; Morris, Wayne L.; Morris, Jenny A.; Suttle, Jeffrey C.; Ramsay, Gavin; Bryan, Glenn J.; Hedley, Pete E.; Taylor, Mark A.
Source:
Plant physiology 2010 v.154 no.2 pp. 656
ISSN:
0032-0889
Subject:
Solanum tuberosum; cultivars; tubers; mature plants; storage organs; carotene 7,8-desaturase; gene expression regulation; microarray technology; RNA interference; transgenic plants; transgenes; carotenoids; violaxanthin; leaves; stems; roots; flowers; potatoes; phenotype; heat tolerance; isoprenoids; cell cleavage; plant breeding
Abstract:
... The factors that regulate storage organ carotenoid content remain to be fully elucidated, despite the nutritional and economic importance of this class of compound. Recent findings suggest that carotenoid pool size is determined, at least in part, by the activity of carotenoid cleavage dioxygenases. The aim of this study was to investigate whether Carotenoid Cleavage Dioxygenase4 (CCD4) activity a ...
Handle:
10113/49244
DOI:
10.1104/pp.110.158733
PubMed:
20688977
PubMed Central:
PMC2949026

30. The MicroRNA159-Regulated GAMYB-like Genes Inhibit Growth and Promote Programmed Cell Death in Arabidopsis

Author:
Alonso-Peral, Maria M.; Li, Junyan; Li, Yanjiao; Allen, Robert S.; Schnippenkoetter, Wendelin; Ohms, Stephen; White, Rosemary G.; Millar, Anthony A.
Source:
Plant physiology 2010 v.154 no.2 pp. 757-771
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; anthers; cell death; cell growth; cell proliferation; flowering; gene expression; genes; seed germination; seeds; transcription factors
Abstract:
... The microRNA159 (miR159) family represses the conserved GAMYB-like genes that encode R2R3 MYB domain transcription factors that have been implicated in gibberellin (GA) signaling in anthers and germinating seeds. In Arabidopsis (Arabidopsis thaliana), the two major miR159 family members, miR159a and miR159b, are functionally specific for two GAMYB-like genes, MYB33 and MYB65. These transcription f ...
DOI:
10.1104/pp.110.160630
PubMed:
20699403
PubMed Central:
PMC2949021

31. The Plant-Specific SR45 Protein Negatively Regulates Glucose and ABA Signaling during Early Seedling Development in Arabidopsis

Author:
Carvalho, Raquel Fonseca; Carvalho, Sofia Domingues; Duque, Paula
Source:
Plant physiology 2010 v.154 no.2 pp. 772-783
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; RNA; abscisic acid; biosynthesis; cotyledons; early development; ethylene; flowers; fluridone; gene expression; genes; glucose; hypersensitivity; leaves; mutants; mutation; nitrogen metabolism; phenotype; plant response; proteins; root growth; seedling growth; seedlings; starch
Abstract:
... The plant-specific SR45 belongs to the highly conserved family of serine/arginine-rich (SR) proteins, which play key roles in precursor-mRNA splicing and other aspects of RNA metabolism. An Arabidopsis (Arabidopsis thaliana) loss-of-function mutant, sr45-1, displays pleiotropic phenotypes, such as defects in flower and leaf morphology, root growth, and flowering time. Here, we show that the sr45-1 ...
DOI:
10.1104/pp.110.155523
PubMed:
20699397
PubMed Central:
PMC2949030

32. The Seed Composition of Arabidopsis Mutants for the Group 3 Sulfate Transporters Indicates a Role in Sulfate Translocation within Developing Seeds

Author:
Zuber, Hélène; Davidian, Jean-Claude; Aubert, Grégoire; Aimé, Delphine; Belghazi, Maya; Lugan, Raphaël; Heintz, Dimitri; Wirtz, Markus; Hell, Rüdiger; Thompson, Richard; Gallardo, Karine
Source:
Plant physiology 2010 v.154 no.2 pp. 913-926
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; amino acids; genes; globulins; mutants; proteome; seeds; sulfates; sulfur; transfer DNA; transporters
Abstract:
... Sulfate is required for the synthesis of sulfur-containing amino acids and numerous other compounds essential for the plant life cycle. The delivery of sulfate to seeds and its translocation between seed tissues is likely to require specific transporters. In Arabidopsis (Arabidopsis thaliana), the group 3 plasmalemma-predicted sulfate transporters (SULTR3) comprise five genes, all expressed in dev ...
DOI:
10.1104/pp.110.162123
PubMed:
20702726
PubMed Central:
PMC2949013

33. Toward in Vivo Chemical Imaging of Epicuticular Waxes

Author:
Weissflog, Ina; Vogler, Nadine; Akimov, Denis; Dellith, Andrea; Schachtschabel, Doreen; Svatos, Ales; Boland, Wilhelm; Dietzek, Benjamin; Popp, Jürgen
Source:
Plant physiology 2010 v.154 no.2 pp. 604-610
ISSN:
0032-0889
Subject:
Hoya carnosa; Monstera; Prunus laurocerasus; epicuticular wax; growth and development; image analysis; in vivo studies; monitoring; plant cuticle; scanning electron microscopy; vibration
Abstract:
... Epicuticular waxes, which are found on the outer surface of plant cuticles, are difficult to study in vivo. To monitor the growth, development, and structural alterations of epicuticular wax layers, coherent anti-Stokes Raman scattering (CARS) might be used. CARS, as a Raman-based technique, not only provides structural insight but also chemical information by imaging the spatial distribution of R ...
DOI:
10.1104/pp.110.161786
PubMed:
20709828
PubMed Central:
PMC2948993

34. Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype

Author:
Diretto, Gianfranco; Al-Babili, Salim; Tavazza, Raffaela; Scossa, Federico; Papacchioli, Velia; Migliore, Melania; Beyer, Peter; Giuliano, Giovanni
Source:
Plant physiology 2010 v.154 no.2 pp. 899-912
ISSN:
0032-0889
Subject:
rice; Oryza sativa; biofortification; Solanum tuberosum; wheat; methodology; patatin; potatoes; genes; tubers; metabolites; phenotype; public health; Triticum aestivum; leaves; Zea mays; corn; vitamin deficiencies; biosynthesis; phytoene synthase
Abstract:
... Vitamin A deficiency is a public health problem in a large number of countries. Biofortification of major staple crops (wheat [Triticum aestivum], rice [Oryza sativa], maize [Zea mays], and potato [Solanum tuberosum]) with β-carotene has the potential to alleviate this nutritional problem. Previously, we engineered transgenic "Golden" potato tubers overexpressing three bacterial genes for β-carote ...
DOI:
10.1104/pp.110.159368
PubMed:
20671108
PubMed Central:
PMC2949014

35. Tyloses and Phenolic Deposits in Xylem Vessels Impede Water Transport in Low-Lignin Transgenic Poplars: A Study by Cryo-Fluorescence Microscopy

Author:
Kitin, Peter; Voelker, Steven L.; Meinzer, Frederick C.; Beeckman, Hans; Strauss, Steven H.; Lachenbruch, Barbara
Source:
Plant physiology 2010 v.154 no.2 pp. 887-898
ISSN:
0032-0889
Subject:
Populus alba; Populus tremula; branches; dyes; freeze drying; genotype; light microscopy; lignin; microscopes; trees; tyloses; wood; xylem vessels
Abstract:
... Of 14 transgenic poplar genotypes (Populus tremula x Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five that had substantial lignin reductions also had greatly reduced xylem-specific conductivity compared with that of control trees and those transgenic events with small reductions in lignin. For the two events with the lowest xylem lignin cont ...
DOI:
10.1104/pp.110.156224
PubMed:
20639405
PubMed Central:
PMC2949004

36. Vernalization-Mediated VIN3 Induction Overcomes the LIKE-HETEROCHROMATIN PROTEIN1/POLYCOMB REPRESSION COMPLEX2-Mediated Epigenetic Repression

Author:
Kim, Dong-Hwan; Zografos, Brett R.; Sung, Sibum
Source:
Plant physiology 2010 v.154 no.2 pp. 949-957
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; chromatin; chronic exposure; epigenetics; flowering; vernalization
Abstract:
... VERNALIZATION INSENSITIVE3 (VIN3) induction by vernalization is one of the earliest events in the vernalization response of Arabidopsis (Arabidopsis thaliana). However, the mechanism responsible for vernalization-mediated VIN3 induction is poorly understood. Here, we show that the constitutive repression of VIN3 in the absence of the cold is due to multiple repressive components, including a trans ...
DOI:
10.1104/pp.110.161083
PubMed:
20671111
PubMed Central:
PMC2948996