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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 2002 Remove constraint Publication Year: 2002 Source 2002 v.130 no.1 Remove constraint Source: 2002 v.130 no.1 Subject chemical constituents of plants Remove constraint Subject: chemical constituents of plants
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1. An endoplasmic reticulum-bound Ca2+/Mn2+ pump, ECA1, supports plant growth and confers tolerance to Mn2+ stress

Author:
Wu, Z.; Liang, F.; Hong, B.; Young, J.C.; Sussman, M.R.; Harper, J.F.; Sze, H.
Source:
Plant physiology 2002 v.130 no.1 pp. 128-137
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; mutants; root hairs; endoplasmic reticulum; plant development; seedling growth; plant proteins; binding proteins; calcium; manganese; ion transport; adenosinetriphosphatase; Saccharomyces cerevisiae; metal tolerance; cations; nucleotide sequences; plant morphology; chemical constituents of plants; translocation (plant physiology); genetic complementation; plant stress; transfer DNA; stress response
Abstract:
... Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms of adaptation are poorly understood. Here, we report the first genetic study to determine in vivo functions of a Ca2+ pump in plants. Homozygous mutants of Arabidopsis harboring a T-DNA disruption in ECA1 showed a 4-fold reduction in endoplasmic reticulum-type calcium pump ac ...
PubMed:
12226493
PubMed Central:
PMC166546

2. Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1

Author:
Kliebenstein, D.J.; Lim, J.E.; Landry, L.G.; Last, R.L.
Source:
Plant physiology 2002 v.130 no.1 pp. 234-243
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; ultraviolet radiation; alleles; biosynthesis; phenylpropanoids; chromosomes; open reading frames; naringenin-chalcone synthase; mutation; chromosome mapping; genetic complementation; recessive genes; flavonoids; chemical constituents of plants; pathogenesis-related proteins; plant morphology
Abstract:
... To further our understanding of how plants defend against the harmful effects of ultraviolet (UV) light, we characterized an Arabidopsis mutant hypersensitive to UV-B. This mutant, UV resistance locus 8-1 (uvr8-1), contains a single recessive mutation at the bottom of chromosome 5. Fine-scale mapping localized uvr8-1 to a 21-kb locus containing five predicted open reading frames. Sequencing of thi ...
PubMed:
12226503
PubMed Central:
PMC166556

3. Arabinoxylan biosynthesis in wheat. Characterization of arabinosyltransferase activity in Golgi membranes

Author:
Porchia, A.C.; Sorensen, S.O.; Scheller, H.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 432-441
ISSN:
0032-0889
Subject:
Triticum aestivum; wheat; food crops; seedlings; Golgi apparatus; microsomes; plant proteins; transferases; enzyme activity; xylan; biosynthesis; pH; manganese; cations; solubilization; carbon; isotopes; isotope labeling; chemical constituents of plants; arabinoxylan
Abstract:
... Arabinoxylan arabinosyltransferase (AX-AraT) activity was investigated using microsomes and Golgi vesicles isolated from wheat (Triticum aestivum) seedlings. Incubation of microsomes with UDP-[14C]-beta-L-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (Xyl), indicating a high degree of UDP-arabinose ( ...
PubMed:
12226522
PubMed Central:
PMC166575

4. A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone

Author:
Mena, M.; Cejudo, F.J.; Isabel-Lamoneda, I.; Carbonero, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 111-119
ISSN:
0032-0889
Subject:
cathepsins; promoter regions; plant proteins; barley; Hordeum vulgare; gibberellic acid; gene expression; gene expression regulation; transcription factors; seed germination; food crops; aleurone cells; endosperm; binding sites; DNA-binding proteins; chemical constituents of plants; pyrimidines; alpha-amylase
Abstract:
... Functional analyses of a number of hydrolase gene promoters, induced by gibberellin (GA) in aleurone cells following germination, have identified a GA-responsive complex as a tripartite element containing a pyrimidine box motif 5'-CCTTTT-3'. We describe here that BPBF, a barley (Hordeum vulgare) transcription factor of the DOF (DNA-Binding with One Finger) class, previously shown to be an activato ...
PubMed:
12226491
PubMed Central:
PMC166544

5. Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance

Author:
Gong, D.; Guo, Y.; Jagendorf, A.T.; Zhu, J.K.
Source:
Plant physiology 2002 v.130 no.1 pp. 256-264
ISSN:
0032-0889
Subject:
manganese; serine; substrate specificity; cations; peptides; phosphorylation; plant proteins; recombinant proteins; gene expression; Arabidopsis thaliana; aspartic acid; mutagenesis; tyrosine; amino acid sequences; enzyme activity; salt tolerance; non-specific serine/threonine protein kinase; kinetics; magnesium; nucleotide sequences; chemical constituents of plants
Abstract:
... The Arabidopsis Salt Overly Sensitive 2 (SOS2) gene encodes a serine/threonine (Thr) protein kinase that has been shown to be a critical component of the salt stress signaling pathway. SOS2 contains a sucrose-non-fermenting protein kinase 1/AMP-activated protein kinase-like N-terminal catalytic domain with an activation loop and a unique C-terminal regulatory domain with an FISL motif that binds t ...
PubMed:
12226505
PubMed Central:
PMC166558

6. Cell-specific expression of homospermidine synthase, the entry enzyme of the pyrrolizidine alkaloid pathway in Senecio vernalis, in comparison with its ancestor, deoxyhypusine synthase

Author:
Moll, S.; Anke, S.; Kahmann, U.; Hansch, R.; Hartmann, T.; Ober, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 47-57
ISSN:
0032-0889
Subject:
plant tissues; Senecio vernalis; messenger RNA; pyrrolizidine alkaloids; biosynthesis; transgenic plants; seedlings; plant proteins; biochemical pathways; gene expression; Nicotiana tabacum; chemical constituents of plants; alkyl (aryl) transferases; plant morphology; roots; chemical structure; tobacco; shoots
Abstract:
... Pyrrolizidine alkaloids (PAs) are constitutive plant defense compounds with a sporadic taxonomic occurrence. The first committed step in PA biosynthesis is catalyzed by homospermidine synthase (HSS). Recent evidence confirmed that HSS evolved by gene duplication from deoxyhypusine synthase (DHS), an enzyme involved in the posttranslational activation of the eukaryotic translation initiation factor ...
PubMed:
12226485
PubMed Central:
PMC166538

7. Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri

Author:
D'Auria, J.C.; Chen, F.; Pichersky, E.
Source:
Plant physiology 2002 v.130 no.1 pp. 466-476
ISSN:
0032-0889
Subject:
stigma; Clarkia; plant proteins; gene expression; Nicotiana tabacum; leaves; Escherichia coli; Arabidopsis thaliana; volatile compounds; complementary DNA; benzyl benzoate; amino acid sequences; enzyme activity; tobacco; immunoblotting; biosynthesis; expressed sequence tags; flowers; biochemical pathways; chemical constituents of plants; protein content; acyltransferases
Abstract:
... A cDNA encoding a protein with 456 amino acids whose sequence shows considerable similarity to plant acyltransferases was identified among 750 Clarkia breweri flower expressed sequence tags. The cDNA was expressed in Escherichia coli, and the protein produced was shown to encode the enzyme benzoyl-coenzyme A (CoA):benzyl alcohol benzoyl transferase (BEBT). BEBT catalyzes the formation of benzylben ...
PubMed:
12226525
PubMed Central:
PMC166578

8. Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue

Author:
Leverentz, M.K.; Wagstaff, C.; Rogers, H.J.; Stead, A.D.; Chanasut, U.; Silkowski, H.; Thomas, B.; Weichert, H.; Feussner, I.; Griffiths, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 273-283
ISSN:
0032-0889
Subject:
electrolytes; corolla; lipid peroxidation; unsaturated fatty acids; plant extracts; cerebrosides; plant proteins; calyx; carotenoids; saturated fatty acids; oxidation; Alstroemeria; stereochemistry; phospholipids; linoleate 13S-lipoxygenase; enzyme activity; antioxidants; membrane permeability; plant tissues; biosynthesis; chemical constituents of plants; chlorophyll
Abstract:
... The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic ac ...
PubMed:
12226507
PubMed Central:
PMC166560

9. Characterization of a strong dominant phytochrome A mutation unique to phytochrome A signal propagation

Author:
Fry, R.C.; Habashi, J.; Okamoto, H.; Deng, X.W.
Source:
Plant physiology 2002 v.130 no.1 pp. 457-465
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; far-red light; seedling growth; alleles; valine; hypocotyls; chemical constituents of plants; seedlings; amino acid substitution; dimerization; chromosome mapping; methionine; nucleotide sequences; kinetics; phytochrome; plant morphology
Abstract:
... Here, we report the isolation and characterization of a strong dominant-negative phytochrome A (phyA) mutation (phyA-300D) in Arabidopsis. This mutation carries a single amino acid substitution at residue 631, from valine to methionine (V631M), in the core region within the C-terminal half of PHYA. This PHYA core region contains two protein-interactive motifs, PAS1 and PAS2. Val-631 is located wit ...
PubMed:
12226524
PubMed Central:
PMC166577

10. Cloning and functional characterization of a beta-pinene synthase from Artemisia annua that shows a circadian pattern of expression

Author:
Lu, S.; Xu, R.; Jia, J.W.; Pang, J.; Matsuda, S.P.T.; Chen, X.Y.
Source:
Plant physiology 2002 v.130 no.1 pp. 477-486
ISSN:
0032-0889
Subject:
plant tissues; biosynthesis; complementary DNA; beta-pinene; amino acid sequences; gene expression; Artemisia annua; diurnal variation; nucleotide sequences; photoperiod; chemical constituents of plants; alkyl (aryl) transferases; emissions; circadian rhythm; light; plant proteins
Abstract:
... Artemisia annua plants produce a broad range of volatile compounds, including monoterpenes, which contribute to the characteristic fragrance of this medicinal species. A cDNA clone, QH6, contained an open reading frame encoding a 582-amino acid protein that showed high sequence identity to plant monoterpene synthases. The prokaryotically expressed QH6 fusion protein converted geranyl diphosphate t ...
PubMed:
12226526
PubMed Central:
PMC166579

11. Developmentally regulated dual-specificity kinase from peanut that is induced by abiotic stresses

Author:
Rudrabhatla, P.; Rajasekharan, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 380-390
ISSN:
0032-0889
Subject:
non-specific serine/threonine protein kinase; protein-tyrosine kinases; Arachis hypogaea; peanuts; plant proteins; complementary DNA; enzyme activity; gene expression; messenger RNA; phosphorylation; cold; sodium chloride; dose response; amino acid sequences; chemical constituents of plants; transcription (genetics); abiotic stress; substrate specificity; oilseed crops; stress response; post-translational modification
Abstract:
... Tyrosine (Tyr) phosphorylation represents an important biochemical mechanism to regulate many cellular processes. No Tyr kinase has been cloned so far in plants. Dual-specificity kinases are reported in plants and the function of these kinases remains unknown. A 1.7-kb cDNA that encodes serine/threonine/Tyr (STY) kinase was isolated by screening peanut (Arachis hypogaea) expression library using t ...
PubMed:
12226517
PubMed Central:
PMC166570

12. Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus

Author:
Lanfranco, L.; Bolchi, A.; Ros, E.C.; Ottonello, S.; Bonfante, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 58-67
ISSN:
0032-0889
Subject:
mycorrhizal fungi; symbionts; spore germination; metal tolerance; plant proteins; polypeptides; cadmium; gene expression; mycelium; sequence analysis; complementary DNA; copper; amino acid sequences; symbiosis; Gigaspora margarita; stress response; expressed sequence tags; metallothionein; genetic complementation; fungal spores; chemical constituents of plants; plant morphology
Abstract:
... A full-length cDNA encoding a metallothionein (MT)-like polypeptide, designated GmarMT1, was identified in an expressed sequence tag collection from germinated spores of the arbuscular mycorrhizal fungus Gigaspora margarita (BEG34). The GmarMT1 gene is composed of two exons separated by an 81-bp intron. It codes for a 65-amino acid polypeptide comprising a plant type 1 MT-like N-terminal domain an ...
PubMed:
12226486
PubMed Central:
PMC166539

13. Do plant have ouabain (OU)-sensitive ATPase?

Author:
Minorsky, P.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 4-5
ISSN:
0032-0889
Subject:
ouabain; enzyme inhibitors; plasma membrane; chemical constituents of plants; adenosinetriphosphatase; plant proteins

14. Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation

Author:
Fang, S.C.; Fernandez, D.E.
Source:
Plant physiology 2002 v.130 no.1 pp. 78-89
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transcription factors; flowering; abscission; seeds; transgenic plants; homeodomain proteins; gene expression regulation; flowers; gene expression; water content; chemical constituents of plants; plant morphology; glucocorticoids; plant proteins
Abstract:
... We have examined the effect of regulated overexpression of AGL15, a member of the MADS domain family of regulatory factors, on reproductive tissues. Using molecular and physiological markers, we show that constitutive overexpression of AGL15 in Arabidopsis leads to delay and down-regulation of senescence programs in perianth organs and developing fruits and alters the process of seed desiccation. ...
PubMed:
12226488
PubMed Central:
PMC166541

15. Engineering herbicide metabolism in tobacco and Arabidopsis with CYP76B1, a cytochrome P450 enzyme from Jerusalem artichoke

Author:
Didierjean, L.; Gondet, L.; Perkins, R.; Lau, S.M.C.; Schaller, H.; O'Keefe, D.P.; Werck-Reichhart, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 179-189
ISSN:
0032-0889
Subject:
Jerusalem artichokes; plant proteins; recombinant proteins; isoproturon; gene expression; Nicotiana tabacum; plant development; metabolism; complementary DNA; amino acid sequences; cytochrome P-450; herbicide resistance; enzyme activity; tobacco; genetic transformation; chlorotoluron; transgenic plants; Saccharomyces cerevisiae; biochemical pathways; oxidoreductases; nucleotide sequences; chemical constituents of plants; plant morphology; Helianthus tuberosus
Abstract:
... The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites. We have found that increased herbicide metabolism and tolerance can be achieved by ectopic constitutive expression of CYP76B1 in tobacco (Nicotiana tabacum) and Arabidopsis. Transformation with CYP7 ...
PubMed:
12226498
PubMed Central:
PMC166551

16. Evidence for a plastid origin of plant ethylene receptor genes

Author:
Mount, S.M.; Chang, C.
Source:
Plant physiology 2002 v.130 no.1 pp. 10-14
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; ethylene; hormone receptors; Anabaena; amino acid sequences; plastids; bacterial proteins; genes; binding sites; chemical constituents of plants; plant proteins
PubMed:
12226482
PubMed Central:
PMC1540253

17. F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from brefeldin A-induced compartments

Author:
Baluska, F.; Hlavacka, A.; Samaj, J.; Palme, K.; Robinson, D.G.; Matoh, T.; McCurdy, D.W.; Menzel, D.; Volkmann, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 422-431
ISSN:
0032-0889
Subject:
developmental stages; endocytosis; roots; endoplasmic reticulum; plant proteins; cell walls; meristems; microfilaments; brefeldin A; plasma membrane; actin; plant development; Zea mays; pectins; exocytosis; corn; cold; Golgi apparatus; protoplasts; food crops; protein synthesis; chemical constituents of plants
Abstract:
... Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but not all, cells. Here, we have analyzed assembly and distribution of BFA compartments in development- and tissue-specific contexts of gro ...
PubMed:
12226521
PubMed Central:
PMC166574

18. Glycerophosphocholine metabolism in higher plant cells. Evidence of a new glyceryl-phosphodiester phosphodiesterase

Author:
Rest, B. van der.; Boisson, A.M.; Gout, E.; Bligny, R.; Douce, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 244-255
ISSN:
0032-0889
Subject:
choline; substrate specificity; vacuoles; plant extracts; spectral analysis; plant proteins; carbohydrate metabolism; phosphoric diester hydrolases; cell walls; Daucus carota; phosphates; carrots; Arabidopsis thaliana; Zea mays; sugar phosphates; forest trees; corn; Acer pseudoplatanus; hydrolysis; ornamental woody plants; enzyme activity; food crops; biochemical pathways; cultured cells; chemical constituents of plants; cell suspension culture
Abstract:
... Glycerophosphocholine (GroPCho) is a diester that accumulates in different physiological processes leading to phospholipid remodeling. However, very little is known about its metabolism in higher plant cells. 31P-Nuclear magnetic resonance spectroscopy and biochemical analyses performed on carrot (Daucus carota) cells fed with GroPCho revealed the existence of an extracellular GroPCho phosphodiest ...
PubMed:
12226504
PubMed Central:
PMC166557

19. Inhibition of squalene synthase and squalene epoxidase in tobacco cells triggers an up-regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Author:
Wentzinger, L.F.; Bach, T.J.; Hartmann, M.A.
Source:
Plant physiology 2002 v.130 no.1 pp. 334-346
ISSN:
0032-0889
Subject:
messenger RNA; biosynthesis; cell growth; enzyme activity; triacylglycerols; cytosol; biochemical pathways; gene expression; enzyme inhibitors; Nicotiana tabacum; chemical constituents of plants; squalene; alcohol oxidoreductases; sterols; cell suspension culture; tobacco; plant proteins
Abstract:
... To get some insight into the regulatory mechanisms controlling the sterol branch of the mevalonate pathway, tobacco (Nicotiana tabacum cv Bright Yellow-2) cell suspensions were treated with squalestatin-1 and terbinafine, two specific inhibitors of squalene synthase (SQS) and squalene epoxidase, respectively. These two enzymes catalyze the first two steps involved in sterol biosynthesis. In highly ...
PubMed:
12226513
PubMed Central:
PMC166566

20. Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C

Author:
Mueller-Roeber, B.; Pical, C.
Source:
Plant physiology 2002 v.130 no.1 pp. 22-46
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; phosphatidylinositol 3-kinase; chemical constituents of plants; 1-phosphatidylinositol 4-kinase; biochemical pathways; amino acid sequences; phospholipase C; genes; phosphatidylinositols; lipid metabolism; isozymes; enzyme activity; plant proteins
Abstract:
... Phosphoinositides (PIs) constitute a minor fraction of total cellular lipids in all eukaryotic cells. They fulfill many important functions through interaction with a wide range of cellular proteins. Members of distinct inositol lipid kinase families catalyze the synthesis of these phospholipids from phosphatidylinositol. The hydrolysis of PIs involves phosphatases and isoforms of PI-specific phos ...
PubMed:
12226484
PubMed Central:
PMC166537