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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 2002 Remove constraint Publication Year: 2002 Source 2002 v.130 no.1 Remove constraint Source: 2002 v.130 no.1
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1. An endoplasmic reticulum-bound Ca2+/Mn2+ pump, ECA1, supports plant growth and confers tolerance to Mn2+ stress

Author:
Wu, Z.; Liang, F.; Hong, B.; Young, J.C.; Sussman, M.R.; Harper, J.F.; Sze, H.
Source:
Plant physiology 2002 v.130 no.1 pp. 128-137
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; mutants; root hairs; endoplasmic reticulum; plant development; seedling growth; plant proteins; binding proteins; calcium; manganese; ion transport; adenosinetriphosphatase; Saccharomyces cerevisiae; metal tolerance; cations; nucleotide sequences; plant morphology; chemical constituents of plants; translocation (plant physiology); genetic complementation; plant stress; transfer DNA; stress response
Abstract:
... Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms of adaptation are poorly understood. Here, we report the first genetic study to determine in vivo functions of a Ca2+ pump in plants. Homozygous mutants of Arabidopsis harboring a T-DNA disruption in ECA1 showed a 4-fold reduction in endoplasmic reticulum-type calcium pump ac ...
PubMed:
12226493
PubMed Central:
PMC166546

2. Arabidopsis CYP98A3 mediating aromatic 3-hydroxylation. Developmental regulation of the gene, and expression in yeast

Author:
Nair, R.B.; Xia, Q.; Kartha, C.J.; Kurylo, E.; Hirji, R.N.; Datla, R.; Selvaraj, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 210-220
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; phenylpropanoids; biosynthesis; stems; caffeic acid; transgenic plants; Saccharomyces cerevisiae; cytochrome P-450; hydroxylation; plant morphology; roots; chemical structure; leaves
Abstract:
... The general phenylpropanoid pathways generate a wide array of aromatic secondary metabolites that range from monolignols, which are ubiquitous in all plants, to sinapine, which is confined to crucifer seeds. The biosynthesis of these compounds involves hydroxylated and methoxylated cinnamyl acid, aldehyde, or alcohol intermediates. Of the three enzymes originally proposed to hydroxylate the 4-, 3- ...
PubMed:
12226501
PubMed Central:
PMC166554

3. Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1

Author:
Kliebenstein, D.J.; Lim, J.E.; Landry, L.G.; Last, R.L.
Source:
Plant physiology 2002 v.130 no.1 pp. 234-243
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; ultraviolet radiation; alleles; biosynthesis; phenylpropanoids; chromosomes; open reading frames; naringenin-chalcone synthase; mutation; chromosome mapping; genetic complementation; recessive genes; flavonoids; chemical constituents of plants; pathogenesis-related proteins; plant morphology
Abstract:
... To further our understanding of how plants defend against the harmful effects of ultraviolet (UV) light, we characterized an Arabidopsis mutant hypersensitive to UV-B. This mutant, UV resistance locus 8-1 (uvr8-1), contains a single recessive mutation at the bottom of chromosome 5. Fine-scale mapping localized uvr8-1 to a 21-kb locus containing five predicted open reading frames. Sequencing of thi ...
PubMed:
12226503
PubMed Central:
PMC166556

4. Arabinoxylan biosynthesis in wheat. Characterization of arabinosyltransferase activity in Golgi membranes

Author:
Porchia, A.C.; Sorensen, S.O.; Scheller, H.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 432-441
ISSN:
0032-0889
Subject:
Triticum aestivum; wheat; food crops; seedlings; Golgi apparatus; microsomes; plant proteins; transferases; enzyme activity; xylan; biosynthesis; pH; manganese; cations; solubilization; carbon; isotopes; isotope labeling; chemical constituents of plants; arabinoxylan
Abstract:
... Arabinoxylan arabinosyltransferase (AX-AraT) activity was investigated using microsomes and Golgi vesicles isolated from wheat (Triticum aestivum) seedlings. Incubation of microsomes with UDP-[14C]-beta-L-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (Xyl), indicating a high degree of UDP-arabinose ( ...
PubMed:
12226522
PubMed Central:
PMC166575

5. A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone

Author:
Mena, M.; Cejudo, F.J.; Isabel-Lamoneda, I.; Carbonero, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 111-119
ISSN:
0032-0889
Subject:
cathepsins; promoter regions; plant proteins; barley; Hordeum vulgare; gibberellic acid; gene expression; gene expression regulation; transcription factors; seed germination; food crops; aleurone cells; endosperm; binding sites; DNA-binding proteins; chemical constituents of plants; pyrimidines; alpha-amylase
Abstract:
... Functional analyses of a number of hydrolase gene promoters, induced by gibberellin (GA) in aleurone cells following germination, have identified a GA-responsive complex as a tripartite element containing a pyrimidine box motif 5'-CCTTTT-3'. We describe here that BPBF, a barley (Hordeum vulgare) transcription factor of the DOF (DNA-Binding with One Finger) class, previously shown to be an activato ...
PubMed:
12226491
PubMed Central:
PMC166544

6. A strobilurin fungicide enhances the resistance of tobacco against tobacco mosaic virus and Pseudomonas syringae pv tabaci

Author:
Herms, S.; Seehaus, K.; Koehle, H.; Conrath, U.
Source:
Plant physiology 2002 v.130 no.1 pp. 120-127
ISSN:
0032-0889
Subject:
Nicotiana tabacum; tobacco; transgenic plants; leaves; unsaturated fatty acids; fungicides; disease resistance; Tobacco mosaic virus; Pseudomonas syringae pv. tabaci; plant pathogenic bacteria; salicylic acid; plant morphology; host plants; plant diseases and disorders; pathogenesis-related proteins
Abstract:
... The strobilurin class of fungicides comprises a variety of synthetic plant-protecting compounds with broad-spectrum antifungal activity. In the present study, we demonstrate that a strobilurin fungicide, F 500 (Pyraclostrobin), enhances the resistance of tobacco (Nicotiana tabacum cv Xanthi nc) against infection by either tobacco mosaic virus (TMV) or the wildfire pathogen Pseudomonas syringae pv ...
PubMed:
12226492
PubMed Central:
PMC166545

7. Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance

Author:
Gong, D.; Guo, Y.; Jagendorf, A.T.; Zhu, J.K.
Source:
Plant physiology 2002 v.130 no.1 pp. 256-264
ISSN:
0032-0889
Subject:
manganese; serine; substrate specificity; cations; peptides; phosphorylation; plant proteins; recombinant proteins; gene expression; Arabidopsis thaliana; aspartic acid; mutagenesis; tyrosine; amino acid sequences; enzyme activity; salt tolerance; non-specific serine/threonine protein kinase; kinetics; magnesium; nucleotide sequences; chemical constituents of plants
Abstract:
... The Arabidopsis Salt Overly Sensitive 2 (SOS2) gene encodes a serine/threonine (Thr) protein kinase that has been shown to be a critical component of the salt stress signaling pathway. SOS2 contains a sucrose-non-fermenting protein kinase 1/AMP-activated protein kinase-like N-terminal catalytic domain with an activation loop and a unique C-terminal regulatory domain with an FISL motif that binds t ...
PubMed:
12226505
PubMed Central:
PMC166558

8. Cell-specific expression of homospermidine synthase, the entry enzyme of the pyrrolizidine alkaloid pathway in Senecio vernalis, in comparison with its ancestor, deoxyhypusine synthase

Author:
Moll, S.; Anke, S.; Kahmann, U.; Hansch, R.; Hartmann, T.; Ober, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 47-57
ISSN:
0032-0889
Subject:
plant tissues; Senecio vernalis; messenger RNA; pyrrolizidine alkaloids; biosynthesis; transgenic plants; seedlings; plant proteins; biochemical pathways; gene expression; Nicotiana tabacum; chemical constituents of plants; alkyl (aryl) transferases; plant morphology; roots; chemical structure; tobacco; shoots
Abstract:
... Pyrrolizidine alkaloids (PAs) are constitutive plant defense compounds with a sporadic taxonomic occurrence. The first committed step in PA biosynthesis is catalyzed by homospermidine synthase (HSS). Recent evidence confirmed that HSS evolved by gene duplication from deoxyhypusine synthase (DHS), an enzyme involved in the posttranslational activation of the eukaryotic translation initiation factor ...
PubMed:
12226485
PubMed Central:
PMC166538

9. Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri

Author:
D'Auria, J.C.; Chen, F.; Pichersky, E.
Source:
Plant physiology 2002 v.130 no.1 pp. 466-476
ISSN:
0032-0889
Subject:
stigma; Clarkia; plant proteins; gene expression; Nicotiana tabacum; leaves; Escherichia coli; Arabidopsis thaliana; volatile compounds; complementary DNA; benzyl benzoate; amino acid sequences; enzyme activity; tobacco; immunoblotting; biosynthesis; expressed sequence tags; flowers; biochemical pathways; chemical constituents of plants; protein content; acyltransferases
Abstract:
... A cDNA encoding a protein with 456 amino acids whose sequence shows considerable similarity to plant acyltransferases was identified among 750 Clarkia breweri flower expressed sequence tags. The cDNA was expressed in Escherichia coli, and the protein produced was shown to encode the enzyme benzoyl-coenzyme A (CoA):benzyl alcohol benzoyl transferase (BEBT). BEBT catalyzes the formation of benzylben ...
PubMed:
12226525
PubMed Central:
PMC166578

10. Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue

Author:
Leverentz, M.K.; Wagstaff, C.; Rogers, H.J.; Stead, A.D.; Chanasut, U.; Silkowski, H.; Thomas, B.; Weichert, H.; Feussner, I.; Griffiths, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 273-283
ISSN:
0032-0889
Subject:
electrolytes; corolla; lipid peroxidation; unsaturated fatty acids; plant extracts; cerebrosides; plant proteins; calyx; carotenoids; saturated fatty acids; oxidation; Alstroemeria; stereochemistry; phospholipids; linoleate 13S-lipoxygenase; enzyme activity; antioxidants; membrane permeability; plant tissues; biosynthesis; chemical constituents of plants; chlorophyll
Abstract:
... The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic ac ...
PubMed:
12226507
PubMed Central:
PMC166560

11. Characterization of a strong dominant phytochrome A mutation unique to phytochrome A signal propagation

Author:
Fry, R.C.; Habashi, J.; Okamoto, H.; Deng, X.W.
Source:
Plant physiology 2002 v.130 no.1 pp. 457-465
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; far-red light; seedling growth; alleles; valine; hypocotyls; chemical constituents of plants; seedlings; amino acid substitution; dimerization; chromosome mapping; methionine; nucleotide sequences; kinetics; phytochrome; plant morphology
Abstract:
... Here, we report the isolation and characterization of a strong dominant-negative phytochrome A (phyA) mutation (phyA-300D) in Arabidopsis. This mutation carries a single amino acid substitution at residue 631, from valine to methionine (V631M), in the core region within the C-terminal half of PHYA. This PHYA core region contains two protein-interactive motifs, PAS1 and PAS2. Val-631 is located wit ...
PubMed:
12226524
PubMed Central:
PMC166577

12. Cloning and functional characterization of a beta-pinene synthase from Artemisia annua that shows a circadian pattern of expression

Author:
Lu, S.; Xu, R.; Jia, J.W.; Pang, J.; Matsuda, S.P.T.; Chen, X.Y.
Source:
Plant physiology 2002 v.130 no.1 pp. 477-486
ISSN:
0032-0889
Subject:
plant tissues; biosynthesis; complementary DNA; beta-pinene; amino acid sequences; gene expression; Artemisia annua; diurnal variation; nucleotide sequences; photoperiod; chemical constituents of plants; alkyl (aryl) transferases; emissions; circadian rhythm; light; plant proteins
Abstract:
... Artemisia annua plants produce a broad range of volatile compounds, including monoterpenes, which contribute to the characteristic fragrance of this medicinal species. A cDNA clone, QH6, contained an open reading frame encoding a 582-amino acid protein that showed high sequence identity to plant monoterpene synthases. The prokaryotically expressed QH6 fusion protein converted geranyl diphosphate t ...
PubMed:
12226526
PubMed Central:
PMC166579

13. Developmentally regulated dual-specificity kinase from peanut that is induced by abiotic stresses

Author:
Rudrabhatla, P.; Rajasekharan, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 380-390
ISSN:
0032-0889
Subject:
non-specific serine/threonine protein kinase; protein-tyrosine kinases; Arachis hypogaea; peanuts; plant proteins; complementary DNA; enzyme activity; gene expression; messenger RNA; phosphorylation; cold; sodium chloride; dose response; amino acid sequences; chemical constituents of plants; transcription (genetics); abiotic stress; substrate specificity; oilseed crops; stress response; post-translational modification
Abstract:
... Tyrosine (Tyr) phosphorylation represents an important biochemical mechanism to regulate many cellular processes. No Tyr kinase has been cloned so far in plants. Dual-specificity kinases are reported in plants and the function of these kinases remains unknown. A 1.7-kb cDNA that encodes serine/threonine/Tyr (STY) kinase was isolated by screening peanut (Arachis hypogaea) expression library using t ...
PubMed:
12226517
PubMed Central:
PMC166570

14. Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus

Author:
Lanfranco, L.; Bolchi, A.; Ros, E.C.; Ottonello, S.; Bonfante, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 58-67
ISSN:
0032-0889
Subject:
mycorrhizal fungi; symbionts; spore germination; metal tolerance; plant proteins; polypeptides; cadmium; gene expression; mycelium; sequence analysis; complementary DNA; copper; amino acid sequences; symbiosis; Gigaspora margarita; stress response; expressed sequence tags; metallothionein; genetic complementation; fungal spores; chemical constituents of plants; plant morphology
Abstract:
... A full-length cDNA encoding a metallothionein (MT)-like polypeptide, designated GmarMT1, was identified in an expressed sequence tag collection from germinated spores of the arbuscular mycorrhizal fungus Gigaspora margarita (BEG34). The GmarMT1 gene is composed of two exons separated by an 81-bp intron. It codes for a 65-amino acid polypeptide comprising a plant type 1 MT-like N-terminal domain an ...
PubMed:
12226486
PubMed Central:
PMC166539

15. Do plant have ouabain (OU)-sensitive ATPase?

Author:
Minorsky, P.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 4-5
ISSN:
0032-0889
Subject:
ouabain; enzyme inhibitors; plasma membrane; chemical constituents of plants; adenosinetriphosphatase; plant proteins

16. Early embryo development in Fucus distichus is auxin sensitive

Author:
Basu, S.; Sun, H.; Brian, L.; Quatrano, R.L.; Muday, G.K.
Source:
Plant physiology 2002 v.130 no.1 pp. 292-302
ISSN:
0032-0889
Subject:
phthalimide; macroalgae; dose response; embryo (plant); naphthylphthalamic acid; cell division; Fucus distichus; gas chromatography; indole acetic acid
Abstract:
... Auxin and polar auxin transport have been implicated in controlling embryo development in land plants. The goal of these studies was to determine if auxin and auxin transport are also important during the earliest stages of development in embryos of the brown alga Fucus distichus. Indole-3-acetic acid (IAA) was identified in F. distichus embryos and mature tissues by gas chromatography-mass spectr ...
PubMed:
12226509
PubMed Central:
PMC166562

17. Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation

Author:
Fang, S.C.; Fernandez, D.E.
Source:
Plant physiology 2002 v.130 no.1 pp. 78-89
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transcription factors; flowering; abscission; seeds; transgenic plants; homeodomain proteins; gene expression regulation; flowers; gene expression; water content; chemical constituents of plants; plant morphology; glucocorticoids; plant proteins
Abstract:
... We have examined the effect of regulated overexpression of AGL15, a member of the MADS domain family of regulatory factors, on reproductive tissues. Using molecular and physiological markers, we show that constitutive overexpression of AGL15 in Arabidopsis leads to delay and down-regulation of senescence programs in perianth organs and developing fruits and alters the process of seed desiccation. ...
PubMed:
12226488
PubMed Central:
PMC166541

18. elongated mesocotyl1, a phytochrome-deficient mutant of maize

Author:
Sawers, R.J.H.; Linley, P.J.; Farmer, P.R.; Hanley, N.P.; Costich, D.E.; Terry, M.J.; Brutnell, T.P.
Source:
Plant physiology 2002 v.130 no.1 pp. 155-163
ISSN:
0032-0889
Subject:
Zea mays; food crops; flowering; heme oxygenase (biliverdin-producing); seedlings; corn; mutants; spectral analysis; etiolation; photosynthesis; phytochrome; plant morphology; enzyme activity
Abstract:
... To begin the functional dissection of light signal transduction pathways of maize (Zea mays), we have identified and characterized the light-sensing mutant elm1 (elongated mesocotyl1). Seedlings homozygous for elm1 are pale green, show pronounced elongation of the mesocotyl, and fail to de-etiolate under red or far-red light. Etiolated elm1 mutants contain no spectrally active phytochrome and do n ...
PubMed:
12226496
PubMed Central:
PMC166549

19. Engineering herbicide metabolism in tobacco and Arabidopsis with CYP76B1, a cytochrome P450 enzyme from Jerusalem artichoke

Author:
Didierjean, L.; Gondet, L.; Perkins, R.; Lau, S.M.C.; Schaller, H.; O'Keefe, D.P.; Werck-Reichhart, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 179-189
ISSN:
0032-0889
Subject:
Jerusalem artichokes; plant proteins; recombinant proteins; isoproturon; gene expression; Nicotiana tabacum; plant development; metabolism; complementary DNA; amino acid sequences; cytochrome P-450; herbicide resistance; enzyme activity; tobacco; genetic transformation; chlorotoluron; transgenic plants; Saccharomyces cerevisiae; biochemical pathways; oxidoreductases; nucleotide sequences; chemical constituents of plants; plant morphology; Helianthus tuberosus
Abstract:
... The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites. We have found that increased herbicide metabolism and tolerance can be achieved by ectopic constitutive expression of CYP76B1 in tobacco (Nicotiana tabacum) and Arabidopsis. Transformation with CYP7 ...
PubMed:
12226498
PubMed Central:
PMC166551

20. Evidence for a plastid origin of plant ethylene receptor genes

Author:
Mount, S.M.; Chang, C.
Source:
Plant physiology 2002 v.130 no.1 pp. 10-14
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; ethylene; hormone receptors; Anabaena; amino acid sequences; plastids; bacterial proteins; genes; binding sites; chemical constituents of plants; plant proteins
PubMed:
12226482
PubMed Central:
PMC1540253

21. F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from brefeldin A-induced compartments

Author:
Baluska, F.; Hlavacka, A.; Samaj, J.; Palme, K.; Robinson, D.G.; Matoh, T.; McCurdy, D.W.; Menzel, D.; Volkmann, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 422-431
ISSN:
0032-0889
Subject:
developmental stages; endocytosis; roots; endoplasmic reticulum; plant proteins; cell walls; meristems; microfilaments; brefeldin A; plasma membrane; actin; plant development; Zea mays; pectins; exocytosis; corn; cold; Golgi apparatus; protoplasts; food crops; protein synthesis; chemical constituents of plants
Abstract:
... Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but not all, cells. Here, we have analyzed assembly and distribution of BFA compartments in development- and tissue-specific contexts of gro ...
PubMed:
12226521
PubMed Central:
PMC166574

22. Glycerophosphocholine metabolism in higher plant cells. Evidence of a new glyceryl-phosphodiester phosphodiesterase

Author:
Rest, B. van der.; Boisson, A.M.; Gout, E.; Bligny, R.; Douce, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 244-255
ISSN:
0032-0889
Subject:
choline; substrate specificity; vacuoles; plant extracts; spectral analysis; plant proteins; carbohydrate metabolism; phosphoric diester hydrolases; cell walls; Daucus carota; phosphates; carrots; Arabidopsis thaliana; Zea mays; sugar phosphates; forest trees; corn; Acer pseudoplatanus; hydrolysis; ornamental woody plants; enzyme activity; food crops; biochemical pathways; cultured cells; chemical constituents of plants; cell suspension culture
Abstract:
... Glycerophosphocholine (GroPCho) is a diester that accumulates in different physiological processes leading to phospholipid remodeling. However, very little is known about its metabolism in higher plant cells. 31P-Nuclear magnetic resonance spectroscopy and biochemical analyses performed on carrot (Daucus carota) cells fed with GroPCho revealed the existence of an extracellular GroPCho phosphodiest ...
PubMed:
12226504
PubMed Central:
PMC166557

23. Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny

Author:
Avsian-Kretchmer, O.; Cheng, J.C.; Chen, L.; Moctezuma, E.; Sung, Z.R.
Source:
Plant physiology 2002 v.130 no.1 pp. 199-209
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; apical meristems; seedling growth; transgenic plants; seedlings; plant vascular system; indole acetic acid; reporter genes; naphthaleneacetic acid; plant morphology; promoter regions; beta-glucuronidase; leaves; organogenesis
Abstract:
... We used an anti-indole acetic acid (IAA or auxin) monoclonal antibody-based immunocytochemical procedure to monitor IAA level in Arabidopsis tissues. Using immunocytochemistry and the IAA-driven beta-glucuronidase (GUS) activity of Aux/IAA promoter::GUS constructs to detect IAA distribution, we investigated the role of polar auxin transport in vascular differentiation during leaf development in Ar ...
PubMed:
12226500
PubMed Central:
PMC166553

24. Inhibition of squalene synthase and squalene epoxidase in tobacco cells triggers an up-regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Author:
Wentzinger, L.F.; Bach, T.J.; Hartmann, M.A.
Source:
Plant physiology 2002 v.130 no.1 pp. 334-346
ISSN:
0032-0889
Subject:
messenger RNA; biosynthesis; cell growth; enzyme activity; triacylglycerols; cytosol; biochemical pathways; gene expression; enzyme inhibitors; Nicotiana tabacum; chemical constituents of plants; squalene; alcohol oxidoreductases; sterols; cell suspension culture; tobacco; plant proteins
Abstract:
... To get some insight into the regulatory mechanisms controlling the sterol branch of the mevalonate pathway, tobacco (Nicotiana tabacum cv Bright Yellow-2) cell suspensions were treated with squalestatin-1 and terbinafine, two specific inhibitors of squalene synthase (SQS) and squalene epoxidase, respectively. These two enzymes catalyze the first two steps involved in sterol biosynthesis. In highly ...
PubMed:
12226513
PubMed Central:
PMC166566

25. Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C

Author:
Mueller-Roeber, B.; Pical, C.
Source:
Plant physiology 2002 v.130 no.1 pp. 22-46
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; phosphatidylinositol 3-kinase; chemical constituents of plants; 1-phosphatidylinositol 4-kinase; biochemical pathways; amino acid sequences; phospholipase C; genes; phosphatidylinositols; lipid metabolism; isozymes; enzyme activity; plant proteins
Abstract:
... Phosphoinositides (PIs) constitute a minor fraction of total cellular lipids in all eukaryotic cells. They fulfill many important functions through interaction with a wide range of cellular proteins. Members of distinct inositol lipid kinase families catalyze the synthesis of these phospholipids from phosphatidylinositol. The hydrolysis of PIs involves phosphatases and isoforms of PI-specific phos ...
PubMed:
12226484
PubMed Central:
PMC166537

26. Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots

Author:
Park, S.W.; Lawrence, C.B.; Linden, J.C.; Vivanco, J.M.
Source:
Plant physiology 2002 v.130 no.1 pp. 164-178
ISSN:
0032-0889
Subject:
ethylene; rhizosphere; cell walls; antifungal properties; plant development; complementary DNA; amino acid sequences; enzyme activity; Trichoderma reesei; Phytolacca americana; N-glycoside hydrolases; tissue culture; transgenic plants; proteinases; root exudates; Saccharomyces cerevisiae; root hairs; nucleotide sequences; chemical constituents of plants; chitinase
Abstract:
... Ribosome-inactivating proteins are N-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rRNA, thus arresting protein synthesis at the translocation step. In the present study, a novel type I ribosome-inactivating protein, termed PAP-H, was purified from Agrobacterium rhizogenes-transformed hairy roots of pokeweed (Phytolacca americana). The protein was purified by ...
PubMed:
12226497
PubMed Central:
PMC166550

27. Light-intensity-dependent expression of Lhc gene family encoding light-harvesting chlorophyll-a/b proteins of photosystem II in Chlamydomonas reinhardtii

Author:
Teramoto, H.; Nakamori, A.; Minagawa, J.; Ono, T.
Source:
Plant physiology 2002 v.130 no.1 pp. 325-333
ISSN:
0032-0889
Subject:
carbon dioxide; messenger RNA; chemical constituents of plants; algae; gene expression regulation; light intensity; electron transfer; gene expression; photosystem II; nucleotide sequences; Chlamydomonas reinhardtii; temperature; chlorophyll; plant proteins
Abstract:
... Excessive light conditions repressed the levels of mRNAs accumulation of multiple Lhc genes encoding light-harvesting chlorophyll-a/b (LHC) proteins of photosystem (PS)II in the unicellular green alga, Chlamydomonas reinhardtii. The light intensity required for the repression tended to decrease with lowering temperature or CO2 concentration. The responses of six LhcII genes encoding the major LHC ...
PubMed:
12226512
PubMed Central:
PMC166565

28. Loss of nuclear gene expression during the phytochrome A-mediated far-red block of greening response

Author:
McCormac, A.C.; Terry, M.J.
Source:
Plant physiology 2002 v.130 no.1 pp. 402-414
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; far-red light; photobleaching; biosynthesis; aldehyde oxidoreductases; white light; transgenic plants; sucrose; biochemical pathways; gene expression; aminolevulinic acid; transcription (genetics); benzyladenine; norflurazon; chemical constituents of plants; phytochrome; gene expression regulation; promoter regions
Abstract:
... We have examined the expression of the HEMA1 gene, which encodes the key chlorophyll synthesis enzyme glutamyl-tRNA reductase, during the phytochrome A-mediated far-red light (FR) block of greening response in Arabidopsis. Our results demonstrate that the FR block of greening comprises two separate responses: a white light (WL) intensity-independent response that requires 3 d of FR and is associat ...
PubMed:
12226519
PubMed Central:
PMC166572

29. Mitochondrial-driven bicarbonate transport supports photosynthesis in a marine microalga

Author:
Huertas, I.E.; Colman, B.; Espie, G.S.
Source:
Plant physiology 2002 v.130 no.1 pp. 284-291
ISSN:
0032-0889
Subject:
macroalgae; carbon dioxide; bicarbonates; Phaeophyceae; translocation (plant physiology); mitochondria; photosynthesis; oxygen; light
Abstract:
... The CO2-concentrating mechanism (CCM) of the marine eustigmatophycean microalga Nannochloropsis gaditana consists of an active HCO3- transport system and an internal carbonic anhydrase to facilitate accumulation and conversion of HCO3- to CO2 for photosynthetic fixation. Aqueous inlet mass spectrometry revealed that a portion of the CO2 generated within the cells leaked to the medium, resulting in ...
PubMed:
12226508
PubMed Central:
PMC166561

30. Molecular identification of cytosolic, patatin-related phospholipases A from Arabidopsis with potential functions in plant signal transduction

Author:
Holk, A.; Rietz, S.; Zahn, M.; Quader, H.; Scherer, G.F.E.
Source:
Plant physiology 2002 v.130 no.1 pp. 90-101
ISSN:
0032-0889
Subject:
seedling growth; phosphatidylcholines; signal transduction; cytosol; patatin; genes; catalytic activity; hypocotyls; recombinant proteins; gene expression; Nicotiana tabacum; gene expression regulation; leaves; Arabidopsis thaliana; messenger RNA; complementary DNA; amino acid sequences; transcription (genetics); enzyme activity; isozymes; tobacco; transgenic plants; biochemical pathways; nucleotide sequences; chemical constituents of plants; esterases; chloroplasts
Abstract:
... Rapid activation of phospholipase A (PLA) by auxin or plant-pathogen interaction suggests a function in signal transduction for this enzyme, but the molecular identification of a cytosolic PLA carrying out this function remains open. We isolated four cDNA sequences from Arabidopsis (ecotype Columbia), AtPLA I, AtPLA IIA, AtPLA IVA, and AtPLA IVC, which are members of the patatin-related PLA gene f ...
PubMed:
12226489
PubMed Central:
PMC166542

31. Movement of potato spindle tuber viroid reveals regulatory points of phloem-mediated RNA traffic

Author:
Zhu, Y.; Qi, Y.; Xun, Y.; Owens, R.; Ding, B.
Source:
Plant physiology 2002 v.130 no.1 pp. 138-146
ISSN:
0032-0889
Subject:
plant development; plant diseases and disorders; transgenic plants; Nicotiana benthamiana; developmental stages; Solanum tuberosum; Potato spindle tuber viroid; flowers; mutagenesis; host plants; phloem; potatoes; Nicotiana tabacum; nucleotide sequences; ultrastructure; RNA; physiological transport; tobacco
Abstract:
... Increasing evidence indicates that the phloem mediates traffic of selective RNAs within a plant. How an RNA enters, moves in, and exits the phloem is poorly understood. Potato spindle tuber viroid (PSTVd) is a pathogenic RNA that does not encode proteins and is not encapsidated, and yet it replicates autonomously and traffics systemically within an infected plant. The viroid RNA genome must intera ...
PubMed:
12226494
PubMed Central:
PMC166547

32. N-acylethanolamines are metabolized by lipoxygenase and amidohydrolase in competing pathways during cottonseed imbibition

Author:
Shrestha, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 391-401
ISSN:
0032-0889
Subject:
cotton; imbibition; fatty acids; biosynthesis; seeds; Gossypium hirsutum; developmental stages; linoleate 13S-lipoxygenase; cottonseed; ethanolamine; biochemical pathways; chemical constituents of plants; lipid metabolism; seed development
Abstract:
... Saturated and unsaturated N-acylethanolamines (NAEs) occur in desiccated seeds primarily as 16C and 18C species with N-palmitoylethanolamine andN-linoleoylethanolamine (NAE 18:2) being most abundant. Here, we examined the metabolic fate of NAEs in vitro and in vivo in imbibed cotton (Gossypium hirsutum) seeds. When synthetic [1-14C]N-palmitoylethanolamine was used as a substrate, free fatty acids ...
PubMed:
12226518
PubMed Central:
PMC166571

33. Nitric oxide negatively modulates wound signaling in tomato plants

Author:
Orozco-Cardenas, M.L.; Ryan, C.A.
Source:
Plant physiology 2002 v.130 no.1 pp. 487-493
ISSN:
0032-0889
Subject:
tomatoes; Solanum lycopersicum var. lycopersicum; messenger RNA; nitric oxide; seedlings; polygalacturonase; linoleate 13S-lipoxygenase; hydrogen peroxide; jasmonic acid; biochemical pathways; gene expression; proteinase inhibitors; chemical constituents of plants; cathepsins; gene expression regulation; leaves; plant proteins
Abstract:
... Synthesis of proteinase inhibitor I protein in response to wounding in leaves of excised tomato (Lycopersiconesculentum) plants was inhibited by NO donors sodium nitroprusside and S-nitroso-N-acetyl-penicillamine. The inhibition was reversed by supplying the plants with the NO scavenger 2-(4-carboxiphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. NO also blocked the hydrogen peroxide (H2O2) ...
PubMed:
12226527
PubMed Central:
PMC166580

34. Patterns of expression and normalized levels of the five Arabidopsis phytochromes

Author:
Sharrock, R.A.; Clack, T.
Source:
Plant physiology 2002 v.130 no.1 pp. 442-456
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant tissues; seed germination; seedling growth; stems; ecotypes; seedlings; DNA; plant extracts; flowers; diurnal variation; light; chemical constituents of plants; phytochrome; circadian rhythm; roots; leaves
Abstract:
... Using monoclonal antibodies specific for each apoprotein and full-length purified apoprotein standards, the levels of the five Arabidopsis phytochromes and their patterns of expression in seedlings and mature plants and under different light conditions have been characterized. Phytochrome levels are normalized to the DNA content of the various tissue extracts to approximate normalization to the nu ...
PubMed:
12226523
PubMed Central:
PMC166576

35. Plant lipoxygenases. Physiological and molecular features

Author:
Porta, H.; Rocha-Sosa, M.
Source:
Plant physiology 2002 v.130 no.1 pp. 15-21
ISSN:
0032-0889
Subject:
seed germination; seeds; seedlings; linoleate 13S-lipoxygenase; biochemical pathways; genes; disease resistance; pest resistance; seed maturation; defense mechanisms
PubMed:
12226483
PubMed Central:
PMC1540254

36. Plasmalemma abscisic acid perception leads to RAB18 expression via phospholipase D activation in Arabidopsis suspension cells

Author:
Hallouin, M.; Ghelis, T.; Brault, M.; Bardat, F.; Cornel, D.; Miginiac, E.; Rona, J.P.; Sotta, B.; Jeannette, E.
Source:
Plant physiology 2002 v.130 no.1 pp. 265-272
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; chemical constituents of plants; calcium; ion transport; signal transduction; biochemical pathways; gene expression; phospholipase C; phospholipase D; plasma membrane; abscisic acid; enzyme activity; cell suspension culture; plant proteins
Abstract:
... Abscisic acid (ABA) plays a key role in the control of stomatal aperture by regulating ion channel activities and water exchanges across the plasma membrane of guard cells. Changes in cytoplasmic calcium content and activation of anion and outward-rectifying K+ channels are among the earliest cellular responses to ABA in guard cells. In Arabidopsis suspension cells, we have demonstrated that outer ...
PubMed:
12226506
PubMed Central:
PMC166559

37. Probing in vivo metabolism by stable isotope labeling of storage lipids and proteins in developing Brassica napus embryos

Author:
Schwender, J.; Ohlrogge, J.B.
Source:
Plant physiology 2002 v.130 no.1 pp. 347-361
ISSN:
0032-0889
Subject:
fatty acids; isotope labeling; cytosol; acetyl coenzyme A; carbon; Brassica napus; malic acid; plant proteins; glucose; isotopes; amino acids; glycolysis; fructose; sucrose; oilseed crops; culture media; biosynthesis; embryo (plant); biochemical pathways; endosperm; plastids; chemical constituents of plants; seed development
Abstract:
... Developing embryos of Brassica napus accumulate both triacylglycerols and proteins as major storage reserves. To evaluate metabolic fluxes during embryo development, we have established conditions for stable isotope labeling of cultured embryos under steady-state conditions. Sucrose supplied via the endosperm is considered to be the main carbon and energy source for seed metabolism. However, in ad ...
PubMed:
12226514
PubMed Central:
PMC166567

38. Regulated expression of Arabidopsis phosphate transporters

Author:
Karthikeyan, A.S.; Varadarajan, D.K.; Mukatira, U.T.; D'Urzo, M.P.; Damsz, B.; Raghothama, K.G.
Source:
Plant physiology 2002 v.130 no.1 pp. 221-233
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transport proteins; transcriptional activation; plant anatomy; seedlings; transgenic plants; nutrient availability; translocation (plant physiology); gene expression; transcription (genetics); auxins; cytokinins; phosphates; plant morphology; promoter regions
Abstract:
... Phosphorus deficiency is one of the major abiotic stresses affecting plant growth. Plants respond to the persistent deficiency of phosphate (Pi) by coordinating the expression of genes involved in alleviation of the stress. The high-affinity Pi transporters are among the major molecular determinants that are activated during Pi stress. In this study, using three reporter genes (green fluorescent p ...
PubMed:
12226502
PubMed Central:
PMC166555

39. Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis

Author:
Bancos, S.; Nomura, T.; Sato, T.; Molnar, G.; Bishop, G.J.; Koncz, C.; Yokota, T.; Nagy, F.; Szekeres, M.
Source:
Plant physiology 2002 v.130 no.1 pp. 504-513
ISSN:
0032-0889
Subject:
seedling growth; spatial distribution; genes; Pisum sativum; roots; shoots; tomatoes; plant proteins; peas; gene expression; gene expression regulation; brassinolide; Arabidopsis thaliana; messenger RNA; evolution; cytochrome P-450; chemical structure; Solanum lycopersicum var. lycopersicum; seed germination; biosynthesis; biochemical pathways; nucleotide sequences; chemical constituents of plants
Abstract:
... Cytochrome P450 enzymes of the closely related CYP90 and CYP85 families catalyze essential oxidative reactions in the biosynthesis of brassinosteroid (BR) hormones. Arabidopsis CYP90B1/DWF4 and CYP90A1/CPD are responsible for respective C-22 and C-23 hydroxylation of the steroid side chain and CYP85A1 catalyzes C-6 oxidation of 6-deoxo intermediates, whereas the functions of CYP90C1/ROT3, CYP90D1, ...
PubMed:
12226529
PubMed Central:
PMC166582

40. Resistance of cultivated tomato to cell content-feeding herbivores is regulated by the octadecanoid-signaling pathway

Author:
Li, C.; Williams, M.M.; Loh, Y.T.; Lee, G.I.; Howe, G.A.
Source:
Plant physiology 2002 v.130 no.1 pp. 494-503
ISSN:
0032-0889
Subject:
tomatoes; Zea mays; food crops; insect pests; tillage; transgenic plants; jasmonic acid; transfer DNA; corn; herbivores; Frankliniella occidentalis; biochemical pathways; chromosome mapping; Tetranychus urticae; pest resistance; transposons; Solanum lycopersicum var. lycopersicum
Abstract:
... The octadecanoid signaling pathway has been shown to play an important role in plant defense against various chewing insects and some pathogenic fungi. Here, we examined the interaction of a cell-content feeding arachnid herbivore, the two-spotted spider mite (Tetranychus urticae Koch), with cultivated tomato (Lycopersicon esculentum) and an isogenic mutant line (defenseless-1 [def-1]) that is def ...
PubMed:
12226528
PubMed Central:
PMC166581

41. Short RNAs can identify new candidate transposable element families in arabidopsis

Author:
Mette, M.F.; Winden, J. van der.; Matzke, M.; Matzke, A.J.M.
Source:
Plant physiology 2002 v.130 no.1 pp. 6-9
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; DNA; gene silencing; double-stranded RNA; chemical constituents of plants; molecular conformation; transposons
PubMed:
12226481
PubMed Central:
PMC1540252

42. Short-term boron deprivation inhibits endocytosis of cell wall pectins in meristematic cells of maize and wheat root apices

Author:
Yu, Q.; Hlavacka, A.; Matoh, T.; Volkmann, D.; Menzel, D.; Goldbach, H.E.; Baluska, F.
Source:
Plant physiology 2002 v.130 no.1 pp. 415-421
ISSN:
0032-0889
Subject:
Zea mays; food crops; fluorescence microscopy; pectins; cell walls; seedlings; meristems; Medicago sativa; corn; wheat; alfalfa; endocytosis; Cucurbita pepo; boron; Triticum aestivum; oilseed crops; chemical constituents of plants; zucchini; roots
Abstract:
... By using immunofluorescence microscopy, we observed rapidly altered distribution patterns of cell wall pectins in meristematic cells of maize (Zea mays) and wheat (Triticum aestivum) root apices. This response was shown for homogalacturonan pectins characterized by a low level (up to 40%) of methylesterification and for rhamnogalacturonan II pectins cross-linked by a borate diol diester. Under bor ...
PubMed:
12226520
PubMed Central:
PMC166573

43. Sterol C-24 methyltransferase type 1 controls the flux of carbon into sterol biosynthesis in tobacco seed

Author:
Holmberg, N.; Harker, M.; Gibbard, C.L.; Wallace, A.D.; Clayton, J.C.; Rawlins, S.; Hellyer, A.; Safford, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 303-311
ISSN:
0032-0889
Subject:
carbon; alcohol oxidoreductases; plant proteins; gene expression; Nicotiana tabacum; leaves; transferases; seeds; campesterol; enzyme activity; chemical structure; tobacco; plant tissues; biosynthesis; transgenic plants; biochemical pathways; cholesterol; nucleotide sequences; chemical constituents of plants
Abstract:
... The first committed step in the conversion of cycloartenol into delta5 C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive carnation etched ring virus promoter or the seed-specific Brassica napus acyl-carri ...
PubMed:
12226510
PubMed Central:
PMC166563

44. Successive glycosyltransfer activity and enzymatic characterization of pectic polygalacturonate 4-alpha-galacturonosyltransferase solubilized from pollen tubes of Petunia axillaris using pyridylaminated oligogalacturonates as substrates

Author:
Akita, K.; Ishimizu, T.; Tsukamoto, T.; Ando, T.; Hase, S.
Source:
Plant physiology 2002 v.130 no.1 pp. 374-379
ISSN:
0032-0889
Subject:
manganese; pectins; Petunia axillaris; cations; pollen tubes; glycogen (starch) synthase; pH; chemical constituents of plants; temperature; enzyme activity; oligosaccharides; solubilization; galacturonic acid; plant proteins
Abstract:
... Polygalacturonate 4-alpha-galacturonosyltransferase (pectin synthase) was solubilized from pollen tubes of Petunia axillaris and characterized. To accomplish this, an assay method using fluorogenic pyridylaminated-oligogalacturonic acids (PA-OGAs) as acceptor substrates was developed. When the pollen tube enzyme was solubilized with 0.5% (v/v) Triton X-100 and was incubated with PA-OGA and UDP-gal ...
PubMed:
12226516
PubMed Central:
PMC166569

45. Tandemly duplicated safener-induced glutathione S-transferase genes from Triticum tauschii contribute to genome- and organ-specific expression in hexaploid wheat

Author:
Xu, F.; Lagudah, E.S.; Moose, S.P.; Riechers, D.E.
Source:
Plant physiology 2002 v.130 no.1 pp. 362-373
ISSN:
0032-0889
Subject:
sequence analysis; food crops; chemical constituents of plants; glutathione transferase; plant proteins; herbicide safeners; wheat; repetitive sequences; gene expression; genes; intergenic DNA; abscisic acid; hexaploidy; roots; Triticum; shoots
Abstract:
... Glutathione S-transferase (GST) gene expression was examined in several Triticum species, differing in genome constitution and ploidy level, to determine genome contribution to GST expression in cultivated, hexaploid bread wheat (Triticum aestivum). Two tandemly duplicated tau class GST genes (TtGSTU1 and TtGSTU2) were isolated from a single bacterial artificial chromosome clone in a library const ...
PubMed:
12226515
PubMed Central:
PMC166568

46. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in the 5'-non-coding region

Author:
Patron, N.J.; Smith, A.M.; Fahy, B.F.; Hylton, C.M.; Naldrett, M.J.; Rossnagel, B.G.; Denyer, K.
Source:
Plant physiology 2002 v.130 no.1 pp. 190-198
ISSN:
0032-0889
Subject:
cultivars; food crops; barley; alleles; messenger RNA; starch granules; Hordeum vulgare; glycosyltransferases; amylose; developmental stages; mutation; amino acid sequences; endosperm; mutants; nucleotide sequences; isozymes; seed development
Abstract:
... Reasons for the variable amylose content of endosperm starch from waxy cultivars of barley (Hordeum vulgare) were investigated. The mature grains of most such cultivars contain some amylose, although amounts are much lower than in wild-type cultivars. In these low-amylose cultivars, amylose synthesis starts relatively late in grain development. Starch granules in the outer cell layers of the endos ...
PubMed:
12226499
PubMed Central:
PMC166552

47. The bifunctional LKR/SDH locus of plants also encodes a highly active monofunctional lysine-ketoglutarate reductase using a polyadenylation signal located within an intron

Author:
Tang, G.; Zhu, X.; Gakiere, B.; Levanony, H.; Kahana, A.; Galili, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 147-154
ISSN:
0032-0889
Subject:
plant proteins; tomatoes; Gossypium hirsutum; gene expression; introns; Arabidopsis thaliana; Zea mays; messenger RNA; abscission; corn; bolls; Solanum lycopersicum var. lycopersicum; cotton; food crops; loci; Saccharomyces cerevisiae; biochemical pathways; saccharopine dehydrogenase; binding sites; chemical constituents of plants
Abstract:
... Both plants and animals catabolize lysine (Lys) via two consecutive enzymes, Lys-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH), which are linked on a single polypeptide encoded by a single LKR/SDH gene. We have previously shown that the Arabidopsis LKR/SDH gene also encodes a monofunctional SDH that is transcribed from an internal promoter. In the present report, we have ident ...
PubMed:
12226495
PubMed Central:
PMC166548

48. The circadian clock that controls gene expression in Arabidopsis in tissue specific

Author:
Thain, S.C.; Murtas, G.; Lynn, J.R.; McGrath, R.B.; Millar, A.J.
Source:
Plant physiology 2002 v.130 no.1 pp. 102-110
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; naringenin-chalcone synthase; transgenic plants; seedlings; luciferase; gene expression regulation; mutation; biochemical pathways; gene expression; plant morphology; circadian rhythm; reporter genes; chemical constituents of plants; mesophyll; roots; promoter regions; leaves; plant proteins
Abstract:
... The expression of CHALCONE SYNTHASE (CHS) expression is an important control step in the biosynthesis of flavonoids, which are major photoprotectants in plants. CHS transcription is regulated by endogenous programs and in response to environmental signals. Luciferase reporter gene fusions showed that the CHS promoter is controlled by the circadian clock both in roots and in aerial organs of transg ...

49. Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis

Author:
Muller, S.; Fuchs, E.; Overcka, M.; Wysocka-Diller, J.; Benfey, P.N.; Hauser, M.T.
Source:
Plant physiology 2002 v.130 no.1 pp. 312-324
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; alleles; loci; seedlings; mutagenesis; cell division; chromosome mapping; plant morphology; mutants; recessive genes; microtubules; chemical constituents of plants; roots; plant proteins
Abstract:
... In screens for regulators of root morphogenesis in Arabidopsis we isolated six new recessive mutants with irregular cell expansion. Complementation analyses placed the mutations in two loci, PLEIADE (PLE) and HYADE (HYA). Phenotypic analyses revealed multinucleated cells, cell wall stubs, and synchronized cell divisions in incompletely separated cells that are all characteristics of defective cyto ...
PubMed:
12226511
PubMed Central:
PMC166564

50. VFL, the grapevine FLORICAULA/LEAFY ortholog, is expressed in meristermatic regions independently of their fate

Author:
Carmona, M.J.; Cubas, P.; Martinez-Zapater, J.M.
Source:
Plant physiology 2002 v.130 no.1 pp. 68-77
ISSN:
0032-0889
Subject:
grapes; reproduction; chemical constituents of plants; developmental stages; meristems; plant proteins; flowers; amino acid sequences; gene expression; seasonal variation; nucleotide sequences; fruit crops; Vitis vinifera; ultrastructure; plant morphology; leaves; vegetative growth
Abstract:
... The flowering process in grapevine (Vitis vinifera) takes place in buds and extends for two consecutive growing seasons. To understand the genetic and molecular mechanisms underlying this process, we have characterized grapevine bud development, cloned the grapevine FLORICAULA/LEAFY (FLO/LFY) ortholog, VFL, and analyzed its expression patterns during vegetative and reproductive development. Flower ...