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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 2002 Remove constraint Publication Year: 2002 Source 2002 v.130 no.1 Remove constraint Source: 2002 v.130 no.1
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1. An endoplasmic reticulum-bound Ca2+/Mn2+ pump, ECA1, supports plant growth and confers tolerance to Mn2+ stress

Author:
Wu, Z.; Liang, F.; Hong, B.; Young, J.C.; Sussman, M.R.; Harper, J.F.; Sze, H.
Source:
Plant physiology 2002 v.130 no.1 pp. 128-137
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; mutants; root hairs; endoplasmic reticulum; plant development; seedling growth; plant proteins; binding proteins; calcium; manganese; ion transport; adenosinetriphosphatase; Saccharomyces cerevisiae; metal tolerance; cations; nucleotide sequences; plant morphology; chemical constituents of plants; translocation (plant physiology); genetic complementation; plant stress; transfer DNA; stress response
Abstract:
... Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms of adaptation are poorly understood. Here, we report the first genetic study to determine in vivo functions of a Ca2+ pump in plants. Homozygous mutants of Arabidopsis harboring a T-DNA disruption in ECA1 showed a 4-fold reduction in endoplasmic reticulum-type calcium pump ac ...
PubMed:
12226493
PubMed Central:
PMC166546

2. Arabidopsis CYP98A3 mediating aromatic 3-hydroxylation. Developmental regulation of the gene, and expression in yeast

Author:
Nair, R.B.; Xia, Q.; Kartha, C.J.; Kurylo, E.; Hirji, R.N.; Datla, R.; Selvaraj, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 210-220
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; phenylpropanoids; biosynthesis; stems; caffeic acid; transgenic plants; Saccharomyces cerevisiae; cytochrome P-450; hydroxylation; plant morphology; roots; chemical structure; leaves
Abstract:
... The general phenylpropanoid pathways generate a wide array of aromatic secondary metabolites that range from monolignols, which are ubiquitous in all plants, to sinapine, which is confined to crucifer seeds. The biosynthesis of these compounds involves hydroxylated and methoxylated cinnamyl acid, aldehyde, or alcohol intermediates. Of the three enzymes originally proposed to hydroxylate the 4-, 3- ...
PubMed:
12226501
PubMed Central:
PMC166554

3. Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1

Author:
Kliebenstein, D.J.; Lim, J.E.; Landry, L.G.; Last, R.L.
Source:
Plant physiology 2002 v.130 no.1 pp. 234-243
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; ultraviolet radiation; alleles; biosynthesis; phenylpropanoids; chromosomes; open reading frames; naringenin-chalcone synthase; mutation; chromosome mapping; genetic complementation; recessive genes; flavonoids; chemical constituents of plants; pathogenesis-related proteins; plant morphology
Abstract:
... To further our understanding of how plants defend against the harmful effects of ultraviolet (UV) light, we characterized an Arabidopsis mutant hypersensitive to UV-B. This mutant, UV resistance locus 8-1 (uvr8-1), contains a single recessive mutation at the bottom of chromosome 5. Fine-scale mapping localized uvr8-1 to a 21-kb locus containing five predicted open reading frames. Sequencing of thi ...
PubMed:
12226503
PubMed Central:
PMC166556

4. Arabinoxylan biosynthesis in wheat. Characterization of arabinosyltransferase activity in Golgi membranes

Author:
Porchia, A.C.; Sorensen, S.O.; Scheller, H.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 432-441
ISSN:
0032-0889
Subject:
Triticum aestivum; wheat; food crops; seedlings; Golgi apparatus; microsomes; plant proteins; transferases; enzyme activity; xylan; biosynthesis; pH; manganese; cations; solubilization; carbon; isotopes; isotope labeling; chemical constituents of plants; arabinoxylan
Abstract:
... Arabinoxylan arabinosyltransferase (AX-AraT) activity was investigated using microsomes and Golgi vesicles isolated from wheat (Triticum aestivum) seedlings. Incubation of microsomes with UDP-[14C]-beta-L-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (Xyl), indicating a high degree of UDP-arabinose ( ...
PubMed:
12226522
PubMed Central:
PMC166575

5. A role for the DOF transcription factor BPBF in the regulation of gibberellin-responsive genes in barley aleurone

Author:
Mena, M.; Cejudo, F.J.; Isabel-Lamoneda, I.; Carbonero, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 111-119
ISSN:
0032-0889
Subject:
cathepsins; promoter regions; plant proteins; barley; Hordeum vulgare; gibberellic acid; gene expression; gene expression regulation; transcription factors; seed germination; food crops; aleurone cells; endosperm; binding sites; DNA-binding proteins; chemical constituents of plants; pyrimidines; alpha-amylase
Abstract:
... Functional analyses of a number of hydrolase gene promoters, induced by gibberellin (GA) in aleurone cells following germination, have identified a GA-responsive complex as a tripartite element containing a pyrimidine box motif 5'-CCTTTT-3'. We describe here that BPBF, a barley (Hordeum vulgare) transcription factor of the DOF (DNA-Binding with One Finger) class, previously shown to be an activato ...
PubMed:
12226491
PubMed Central:
PMC166544

6. A strobilurin fungicide enhances the resistance of tobacco against tobacco mosaic virus and Pseudomonas syringae pv tabaci

Author:
Herms, S.; Seehaus, K.; Koehle, H.; Conrath, U.
Source:
Plant physiology 2002 v.130 no.1 pp. 120-127
ISSN:
0032-0889
Subject:
Nicotiana tabacum; tobacco; transgenic plants; leaves; unsaturated fatty acids; fungicides; disease resistance; Tobacco mosaic virus; Pseudomonas syringae pv. tabaci; plant pathogenic bacteria; salicylic acid; plant morphology; host plants; plant diseases and disorders; pathogenesis-related proteins
Abstract:
... The strobilurin class of fungicides comprises a variety of synthetic plant-protecting compounds with broad-spectrum antifungal activity. In the present study, we demonstrate that a strobilurin fungicide, F 500 (Pyraclostrobin), enhances the resistance of tobacco (Nicotiana tabacum cv Xanthi nc) against infection by either tobacco mosaic virus (TMV) or the wildfire pathogen Pseudomonas syringae pv ...
PubMed:
12226492
PubMed Central:
PMC166545

7. Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance

Author:
Gong, D.; Guo, Y.; Jagendorf, A.T.; Zhu, J.K.
Source:
Plant physiology 2002 v.130 no.1 pp. 256-264
ISSN:
0032-0889
Subject:
manganese; serine; substrate specificity; cations; peptides; phosphorylation; plant proteins; recombinant proteins; gene expression; Arabidopsis thaliana; aspartic acid; mutagenesis; tyrosine; amino acid sequences; enzyme activity; salt tolerance; non-specific serine/threonine protein kinase; kinetics; magnesium; nucleotide sequences; chemical constituents of plants
Abstract:
... The Arabidopsis Salt Overly Sensitive 2 (SOS2) gene encodes a serine/threonine (Thr) protein kinase that has been shown to be a critical component of the salt stress signaling pathway. SOS2 contains a sucrose-non-fermenting protein kinase 1/AMP-activated protein kinase-like N-terminal catalytic domain with an activation loop and a unique C-terminal regulatory domain with an FISL motif that binds t ...
PubMed:
12226505
PubMed Central:
PMC166558

8. Cell-specific expression of homospermidine synthase, the entry enzyme of the pyrrolizidine alkaloid pathway in Senecio vernalis, in comparison with its ancestor, deoxyhypusine synthase

Author:
Moll, S.; Anke, S.; Kahmann, U.; Hansch, R.; Hartmann, T.; Ober, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 47-57
ISSN:
0032-0889
Subject:
plant tissues; Senecio vernalis; messenger RNA; pyrrolizidine alkaloids; biosynthesis; transgenic plants; seedlings; plant proteins; biochemical pathways; gene expression; Nicotiana tabacum; chemical constituents of plants; alkyl (aryl) transferases; plant morphology; roots; chemical structure; tobacco; shoots
Abstract:
... Pyrrolizidine alkaloids (PAs) are constitutive plant defense compounds with a sporadic taxonomic occurrence. The first committed step in PA biosynthesis is catalyzed by homospermidine synthase (HSS). Recent evidence confirmed that HSS evolved by gene duplication from deoxyhypusine synthase (DHS), an enzyme involved in the posttranslational activation of the eukaryotic translation initiation factor ...
PubMed:
12226485
PubMed Central:
PMC166538

9. Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri

Author:
D'Auria, J.C.; Chen, F.; Pichersky, E.
Source:
Plant physiology 2002 v.130 no.1 pp. 466-476
ISSN:
0032-0889
Subject:
stigma; Clarkia; plant proteins; gene expression; Nicotiana tabacum; leaves; Escherichia coli; Arabidopsis thaliana; volatile compounds; complementary DNA; benzyl benzoate; amino acid sequences; enzyme activity; tobacco; immunoblotting; biosynthesis; expressed sequence tags; flowers; biochemical pathways; chemical constituents of plants; protein content; acyltransferases
Abstract:
... A cDNA encoding a protein with 456 amino acids whose sequence shows considerable similarity to plant acyltransferases was identified among 750 Clarkia breweri flower expressed sequence tags. The cDNA was expressed in Escherichia coli, and the protein produced was shown to encode the enzyme benzoyl-coenzyme A (CoA):benzyl alcohol benzoyl transferase (BEBT). BEBT catalyzes the formation of benzylben ...
PubMed:
12226525
PubMed Central:
PMC166578

10. Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue

Author:
Leverentz, M.K.; Wagstaff, C.; Rogers, H.J.; Stead, A.D.; Chanasut, U.; Silkowski, H.; Thomas, B.; Weichert, H.; Feussner, I.; Griffiths, G.
Source:
Plant physiology 2002 v.130 no.1 pp. 273-283
ISSN:
0032-0889
Subject:
electrolytes; corolla; lipid peroxidation; unsaturated fatty acids; plant extracts; cerebrosides; plant proteins; calyx; carotenoids; saturated fatty acids; oxidation; Alstroemeria; stereochemistry; phospholipids; linoleate 13S-lipoxygenase; enzyme activity; antioxidants; membrane permeability; plant tissues; biosynthesis; chemical constituents of plants; chlorophyll
Abstract:
... The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic ac ...
PubMed:
12226507
PubMed Central:
PMC166560

11. Characterization of a strong dominant phytochrome A mutation unique to phytochrome A signal propagation

Author:
Fry, R.C.; Habashi, J.; Okamoto, H.; Deng, X.W.
Source:
Plant physiology 2002 v.130 no.1 pp. 457-465
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; far-red light; seedling growth; alleles; valine; hypocotyls; chemical constituents of plants; seedlings; amino acid substitution; dimerization; chromosome mapping; methionine; nucleotide sequences; kinetics; phytochrome; plant morphology
Abstract:
... Here, we report the isolation and characterization of a strong dominant-negative phytochrome A (phyA) mutation (phyA-300D) in Arabidopsis. This mutation carries a single amino acid substitution at residue 631, from valine to methionine (V631M), in the core region within the C-terminal half of PHYA. This PHYA core region contains two protein-interactive motifs, PAS1 and PAS2. Val-631 is located wit ...
PubMed:
12226524
PubMed Central:
PMC166577

12. Cloning and functional characterization of a beta-pinene synthase from Artemisia annua that shows a circadian pattern of expression

Author:
Lu, S.; Xu, R.; Jia, J.W.; Pang, J.; Matsuda, S.P.T.; Chen, X.Y.
Source:
Plant physiology 2002 v.130 no.1 pp. 477-486
ISSN:
0032-0889
Subject:
plant tissues; biosynthesis; complementary DNA; beta-pinene; amino acid sequences; gene expression; Artemisia annua; diurnal variation; nucleotide sequences; photoperiod; chemical constituents of plants; alkyl (aryl) transferases; emissions; circadian rhythm; light; plant proteins
Abstract:
... Artemisia annua plants produce a broad range of volatile compounds, including monoterpenes, which contribute to the characteristic fragrance of this medicinal species. A cDNA clone, QH6, contained an open reading frame encoding a 582-amino acid protein that showed high sequence identity to plant monoterpene synthases. The prokaryotically expressed QH6 fusion protein converted geranyl diphosphate t ...
PubMed:
12226526
PubMed Central:
PMC166579

13. Developmentally regulated dual-specificity kinase from peanut that is induced by abiotic stresses

Author:
Rudrabhatla, P.; Rajasekharan, R.
Source:
Plant physiology 2002 v.130 no.1 pp. 380-390
ISSN:
0032-0889
Subject:
non-specific serine/threonine protein kinase; protein-tyrosine kinases; Arachis hypogaea; peanuts; plant proteins; complementary DNA; enzyme activity; gene expression; messenger RNA; phosphorylation; cold; sodium chloride; dose response; amino acid sequences; chemical constituents of plants; transcription (genetics); abiotic stress; substrate specificity; oilseed crops; stress response; post-translational modification
Abstract:
... Tyrosine (Tyr) phosphorylation represents an important biochemical mechanism to regulate many cellular processes. No Tyr kinase has been cloned so far in plants. Dual-specificity kinases are reported in plants and the function of these kinases remains unknown. A 1.7-kb cDNA that encodes serine/threonine/Tyr (STY) kinase was isolated by screening peanut (Arachis hypogaea) expression library using t ...
PubMed:
12226517
PubMed Central:
PMC166570

14. Differential expression of a metallothionein gene during the presymbiotic versus the symbiotic phase of an arbuscular mycorrhizal fungus

Author:
Lanfranco, L.; Bolchi, A.; Ros, E.C.; Ottonello, S.; Bonfante, P.
Source:
Plant physiology 2002 v.130 no.1 pp. 58-67
ISSN:
0032-0889
Subject:
mycorrhizal fungi; symbionts; spore germination; metal tolerance; plant proteins; polypeptides; cadmium; gene expression; mycelium; sequence analysis; complementary DNA; copper; amino acid sequences; symbiosis; Gigaspora margarita; stress response; expressed sequence tags; metallothionein; genetic complementation; fungal spores; chemical constituents of plants; plant morphology
Abstract:
... A full-length cDNA encoding a metallothionein (MT)-like polypeptide, designated GmarMT1, was identified in an expressed sequence tag collection from germinated spores of the arbuscular mycorrhizal fungus Gigaspora margarita (BEG34). The GmarMT1 gene is composed of two exons separated by an 81-bp intron. It codes for a 65-amino acid polypeptide comprising a plant type 1 MT-like N-terminal domain an ...
PubMed:
12226486
PubMed Central:
PMC166539

15. Do plant have ouabain (OU)-sensitive ATPase?

Author:
Minorsky, P.V.
Source:
Plant physiology 2002 v.130 no.1 pp. 4-5
ISSN:
0032-0889
Subject:
ouabain; enzyme inhibitors; plasma membrane; chemical constituents of plants; adenosinetriphosphatase; plant proteins

16. Early embryo development in Fucus distichus is auxin sensitive

Author:
Basu, S.; Sun, H.; Brian, L.; Quatrano, R.L.; Muday, G.K.
Source:
Plant physiology 2002 v.130 no.1 pp. 292-302
ISSN:
0032-0889
Subject:
phthalimide; macroalgae; dose response; embryo (plant); naphthylphthalamic acid; cell division; Fucus distichus; gas chromatography; indole acetic acid
Abstract:
... Auxin and polar auxin transport have been implicated in controlling embryo development in land plants. The goal of these studies was to determine if auxin and auxin transport are also important during the earliest stages of development in embryos of the brown alga Fucus distichus. Indole-3-acetic acid (IAA) was identified in F. distichus embryos and mature tissues by gas chromatography-mass spectr ...
PubMed:
12226509
PubMed Central:
PMC166562

17. Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation

Author:
Fang, S.C.; Fernandez, D.E.
Source:
Plant physiology 2002 v.130 no.1 pp. 78-89
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transcription factors; flowering; abscission; seeds; transgenic plants; homeodomain proteins; gene expression regulation; flowers; gene expression; water content; chemical constituents of plants; plant morphology; glucocorticoids; plant proteins
Abstract:
... We have examined the effect of regulated overexpression of AGL15, a member of the MADS domain family of regulatory factors, on reproductive tissues. Using molecular and physiological markers, we show that constitutive overexpression of AGL15 in Arabidopsis leads to delay and down-regulation of senescence programs in perianth organs and developing fruits and alters the process of seed desiccation. ...
PubMed:
12226488
PubMed Central:
PMC166541

18. elongated mesocotyl1, a phytochrome-deficient mutant of maize

Author:
Sawers, R.J.H.; Linley, P.J.; Farmer, P.R.; Hanley, N.P.; Costich, D.E.; Terry, M.J.; Brutnell, T.P.
Source:
Plant physiology 2002 v.130 no.1 pp. 155-163
ISSN:
0032-0889
Subject:
Zea mays; food crops; flowering; heme oxygenase (biliverdin-producing); seedlings; corn; mutants; spectral analysis; etiolation; photosynthesis; phytochrome; plant morphology; enzyme activity
Abstract:
... To begin the functional dissection of light signal transduction pathways of maize (Zea mays), we have identified and characterized the light-sensing mutant elm1 (elongated mesocotyl1). Seedlings homozygous for elm1 are pale green, show pronounced elongation of the mesocotyl, and fail to de-etiolate under red or far-red light. Etiolated elm1 mutants contain no spectrally active phytochrome and do n ...
PubMed:
12226496
PubMed Central:
PMC166549

19. Engineering herbicide metabolism in tobacco and Arabidopsis with CYP76B1, a cytochrome P450 enzyme from Jerusalem artichoke

Author:
Didierjean, L.; Gondet, L.; Perkins, R.; Lau, S.M.C.; Schaller, H.; O'Keefe, D.P.; Werck-Reichhart, D.
Source:
Plant physiology 2002 v.130 no.1 pp. 179-189
ISSN:
0032-0889
Subject:
Jerusalem artichokes; plant proteins; recombinant proteins; isoproturon; gene expression; Nicotiana tabacum; plant development; metabolism; complementary DNA; amino acid sequences; cytochrome P-450; herbicide resistance; enzyme activity; tobacco; genetic transformation; chlorotoluron; transgenic plants; Saccharomyces cerevisiae; biochemical pathways; oxidoreductases; nucleotide sequences; chemical constituents of plants; plant morphology; Helianthus tuberosus
Abstract:
... The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites. We have found that increased herbicide metabolism and tolerance can be achieved by ectopic constitutive expression of CYP76B1 in tobacco (Nicotiana tabacum) and Arabidopsis. Transformation with CYP7 ...
PubMed:
12226498
PubMed Central:
PMC166551

20. Evidence for a plastid origin of plant ethylene receptor genes

Author:
Mount, S.M.; Chang, C.
Source:
Plant physiology 2002 v.130 no.1 pp. 10-14
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; ethylene; hormone receptors; Anabaena; amino acid sequences; plastids; bacterial proteins; genes; binding sites; chemical constituents of plants; plant proteins
PubMed:
12226482
PubMed Central:
PMC1540253