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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 2001 Remove constraint Publication Year: 2001 Source 2001 v.126 no.4 Remove constraint Source: 2001 v.126 no.4 Subject amino acid sequences Remove constraint Subject: amino acid sequences
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1. A Medicago truncatula homoglutathione synthetase is derived from glutathione synthetase by gene duplication

Author:
Frendo, P.; Jimenez, M.J.H.; Mathieu, C.; Duret, L.; Gallesi, D.; Van de Sype, G.; Herouart, D.; Puppo, A.
Source:
Plant physiology 2001 v.126 no.4 pp. 1706-1715
ISSN:
0032-0889
Subject:
glutathione; leucine; complementary DNA; molecular weight; proline; alanine; phylogeny; amino acid sequences; gene expression; genes; nucleotide sequences; genetic complementation; glutathione synthase; glycine (amino acid); Medicago truncatula
Abstract:
... Glutathione (GSH) and homo-GSH (hGSH) are the major low-molecular weight thiols synthesized in Medicago truncatula. Two M. truncatula cDNAs (gshs1 and gshs2) corresponding to a putative GSH synthetase (GSHS) and a putative hGSH synthetase (hGSHS) were characterized. Heterologous expression of gshs1 and gshs2 cDNAs in an Escherichia coli strain deficient in GSHS activity showed that GSHS1 and GSHS2 ...
PubMed:
11500568
PubMed Central:
PMC117169

2. A novel superoxide dismutase with a high isoelectric point in higher plants. Expression, regulation, and protein localization

Author:
Karpinska, B.; Karlsson, M.; Schinkel, H.; Streller, S.; Suss, K.H.; Melzer, M.; Wingsle, G.
Source:
Plant physiology 2001 v.126 no.4 pp. 1668-1677
ISSN:
0032-0889
Subject:
immunocytochemistry; sclerenchyma; messenger RNA; complementary DNA; immunohistochemistry; Golgi apparatus; cell walls; superoxide dismutase; isoelectric point; amino acid sequences; conifer needles; gene expression; cytosol; Pinus sylvestris; nucleotide sequences; plasma membrane; chemical constituents of plants; xylem; isozymes; chloroplasts
Abstract:
... Several isoforms of superoxide dismutase (SOD) with a high isoelectric point (pI) have been identified by isoelectric focusing chromatography in protein extracts from Scots pine (Pinus sylvestris) needles. One of these isoforms, a CuZn-SOD with a pI of about 10 and thus denoted hipI-SOD, has been isolated and purified to apparent homogeneity. A cDNA encoding the hipI-SOD protein was cloned and seq ...
PubMed:
11500564
PubMed Central:
PMC117165

3. Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1

Author:
Tozawa, Y.; Hasegawa, H.; Terakawa, T.; Wakasa, K.
Source:
Plant physiology 2001 v.126 no.4 pp. 1493-1506
ISSN:
0032-0889
Subject:
Oryza sativa; genes; nucleotide sequences; transgenic plants; mutants; tryptophan; phylogeny; gene expression; metabolism; callus; amino acid sequences; indole acetic acid
Abstract:
... Anthranilate synthase (AS) is a key enzyme in the synthesis of tryptophan (Trp), indole-3-acetic acid, and indole alkaloids. Two genes, OASA1 and OASA2, encoding AS alpha-subunits were isolated from a monocotyledonous plant, rice (Oryza sativa cv Nipponbare), and were characterized. A phylogenetic tree of AS alpha-subunits from various species revealed a close evolutionary relationship among OASA1 ...
PubMed:
11500548
PubMed Central:
PMC117149

4. Cloning and characterization of a coronatine-regulated tyrosine aminotransferase from Arabidopsis

Author:
Lopukhina, A.; Dettenberg, M.; Weiler, E.W.; Hollander-Czytko, H.
Source:
Plant physiology 2001 v.126 no.4 pp. 1678-1687
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; tyrosine transaminase; enzyme activity; phytotoxins; plant physiology; metabolites; RNA; complementary DNA; nucleotide sequences; amino acid sequences; molecular weight; mechanical damage
Abstract:
... In plants, the phytotoxin coronatine, which is an analog of the octadecanoids 12-oxo-phytodienoic acid and/or jasmonic acid, gives rise to a number of physiological responses similar to those of octadecanoids. To further elucidate the physiological role of these compounds, the differential RNA display technique was used to isolate a number of novel octadecanoid-inducible genes expressed in coronat ...
PubMed:
11500565
PubMed Central:
PMC117166

5. Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis abundant proteins

Author:
Ukaji, N.; Kuwabara, C.; Takezawa, D.; Arakawa, K.; Fujikawa, S.
Source:
Plant physiology 2001 v.126 no.4 pp. 1588-1597
ISSN:
0032-0889
Subject:
cortex; messenger RNA; complementary DNA; amino acid sequences; nucleotide sequences; Morus bombycis; molecular weight; immunocytochemistry; cold tolerance; endoplasmic reticulum; plant proteins
Abstract:
... We have shown that two 27-kD proteins, designated as WAP27A and WAP27B, were abundantly accumulated in endoplasmic reticulum-enriched fractions isolated from cortical parenchyma cells of mulberry tree (Morus bombycis Koidz.) during winter (N. Ukaji, C. Kuwabara, D. Takezawa, K. Arakawa, S. Yoshida, S. Fujikawa [1999] Plant Physiol 120: 480-489). In the present study, cDNA clones encoding WAP27A an ...
PubMed:
11500557
PubMed Central:
PMC117158

6. Efficient prenylation by a plant geranylgeranyl-transferase-I requires a functional caaL box motif and a proximal polybasic domain

Author:
Caldelari, D.; Sternberg, H.; Rodriguez-Concepcion, M.; Gruissem, W.; Yalovsky, S.
Source:
Plant physiology 2001 v.126 no.4 pp. 1416-1429
ISSN:
0032-0889
Subject:
plant tissues; polymerase chain reaction; Saccharomyces cerevisiae; amino acid sequences; gene expression; pH; mutants; alkyl (aryl) transferases; enzyme activity; Solanum lycopersicum var. lycopersicum
Abstract:
... Geranylgeranyltransferase-I (GGT-I) is a heterodimeric enzyme that shares a common alpha-subunit with farnesyltransferase (FTase) and has a distinct beta-subunit. GGT-I preferentially modifies proteins, whichterminate in a CaaL box sequence motif. Cloning of Arabidopsis GGT-I beta-subunit (AtGGT-IB) was achieved by a yeast (Saccharomyces cerevisiae) two-hybrid screen, using the tomato (Lycopersico ...
PubMed:
11500541
PubMed Central:
PMC117142

7. Isolation and characterization of kinase interacting protein 1, a pollen protein that interacts with the kinase domain of PRK1, a receptor-like kinase of petunia

Author:
Skirpan, A.L.; McCubbin, A.G.; Ishimizu, T.; Wang, X.; Hu, Y.; Dowd, P.E.; Ma, H.; Kao, T.
Source:
Plant physiology 2001 v.126 no.4 pp. 1480-1492
ISSN:
0032-0889
Subject:
Petunia integrifolia; pollen; plant proteins; plant physiology; cytosol; antisense RNA; sexual reproduction; spores; biochemical pathways; complementary DNA; nucleotide sequences; amino acid sequences; nucleic acid hybridization
Abstract:
... Many receptor-like kinases have been identified in plants and have been shown by genetic or transgenic knockouts to play diverse physiological roles; however, to date, the cytosolic interacting proteins of relatively few of these kinases have been identified. We have previously identified a predominantly pollen-expressed receptor-like kinase of petunia (Petunia inflata), named PRK1, and we have sh ...
PubMed:
11500547
PubMed Central:
PMC117148

8. Phylogenetic relationships within cation transporter families of Arabidopsis

Author:
Maser, P.; Thomine, S.; Schroeder, J.I.; Ward, J.M.; Hirschi, K.; Sze, H.; Talke, I.N.; Amtmann, A.; Maathuis, F.J.M.; Sanders, D.
Source:
Plant physiology 2001 v.126 no.4 pp. 1646-1667
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; phylogeny; cations; ion transport; plant proteins; amino acid sequences; nucleotide sequences; isomers; multigene family; genomics; chromosome mapping
Abstract:
... Uptake and translocation of cationic nutrients play essential roles in physiological processes including plant growth, nutrition, signal transduction, and development. Approximately 5% of the Arabidopsis genome appears to encode membrane transport proteins. These proteins are classified in 46 unique families containing approximately 880 members. In addition, several hundred putative transporters h ...
PubMed:
11500563
PubMed Central:
PMC117164

9. Physiological and molecular biological characterization of intracellular carbonic anhydrase from the marine diatom Phaeodactylum tricornutum

Author:
Satoh, D.; Hiraoka, Y.; Colman, B.; Matsuda, Y.
Source:
Plant physiology 2001 v.126 no.4 pp. 1459-1470
ISSN:
0032-0889
Subject:
carbon dioxide; carbonate dehydratase; amino acid sequences; enzyme inhibitors; nucleotide sequences; photosynthesis; molecular weight; molecular biology; Bacillariophyta
Abstract:
... A single intracellular carbonic anhydrase (CA) was detected in air-grown and, at reduced levels, in high CO2-grown cells of the marine diatom Phaeodactylum tricornutum (UTEX 642). No external CA activity was detected irrespective of growth CO2 conditions. Ethoxyzolamide (0.4 mM), a CA-specific inhibitor, severely inhibited high-affinity photosynthesis at low concentrations of dissolved inorganic c ...
PubMed:
11500545
PubMed Central:
PMC117146

10. Sequence architecture downstream of the initiator codon enhances gene expression and protein stability in plants

Author:
Sawant, S.V.; Kiran, K.; Singh, P.K.; Tuli, R.
Source:
Plant physiology 2001 v.126 no.4 pp. 1630-1636
ISSN:
0032-0889
Subject:
green fluorescent protein; animal proteins; amino acid sequences; codons; gene expression; transcription (genetics); Nicotiana tabacum; reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... Nucleotide positions conserved on the 3' side of the initiator codon ATG and the corresponding N-terminal amino acid residues in a number of highly abundant plant proteins were identified by computational analysis of a dataset of highly expressed plant genes. The reporter genes uidA and gfp were modified to introduce these features. Insertion of GCT TCC TCC after the initiator codon ATG augmented ...
PubMed:
11500561
PubMed Central:
PMC117162