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You searched for: Journal name Plant physiology Remove constraint Journal name: Plant physiology Publication year rev 8000-2000 Remove constraint Publication year rev: 8000-2000 Source 2000 v.124 no.3 Remove constraint Source: 2000 v.124 no.3
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1. Analysis of oxidative stress and wound-inducible dinor isoprostanes F1 (phytoprostanes F1) in plants

Author:
Imbusch, R.; Mueller, M.J.
Source:
Plant physiology 2000 v.124 no.3 pp. 1293-1303
ISSN:
0032-0889
Subject:
Mentha piperita; leaves; oxidation; arachidonic acid; metabolites; linolenic acid; quantitative analysis; biochemical pathways; chemical constituents of plants; stress response
Abstract:
... Isoprostanes F2 are arachidonate autoxidation products in mammals that have been shown to be induced during several human disorders associated with enhanced free-radical generation. Isoprostanes F2 represent not only extremely reliable markers of oxidative stress in vivo, but they also exert potent biological effects. Therefore, it has been postulated that isoprostanoids are mediators of oxidant i ...
PubMed:
11080305
PubMed Central:
PMC59227

2. Cell surface expansion in polarly growing root hairs of Medicago truncatula

Author:
Shaw, S.L.; Dumais, J.; Long, S.R.
Source:
Plant physiology 2000 v.124 no.3 pp. 959-969
ISSN:
0032-0889
Subject:
cell growth; developmental stages; root hairs; length; Medicago truncatula; measurement
Abstract:
... Fluorescent microspheres were used as material markers to investigate the relative rates of cell surface expansion at the growing tips of Medicago truncatula root hairs. From the analysis of tip shape and microsphere movements, we propose three characteristic zones of expansion in growing root hairs. The center of the apical dome is an area of 1- to 2-micrometer diameter with relatively constant c ...
PubMed:
11080274
PubMed Central:
PMC59196

3. Characterization of the two saccharopine dehydrogenase isozymes of lysine catabolism encoded by the single composite AtLKR/SDH locus of Arabidopsis

Author:
Zhu, X.; Tang, G.; Galili, G.
Source:
Plant physiology 2000 v.124 no.3 pp. 1363-1371
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; cytosol; lysine; metabolism; saccharopine dehydrogenase; isozymes; enzyme activity; biochemical pathways; pH; genes; genetic code; loci; gene expression; transgenic plants; genetic variation; genotype
Abstract:
... Arabidopsis plants possess a composite AtLKR/SDH locus encoding two different polypeptides involved in lysine catabolism: a bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH) enzyme and a monofunctional SDH enzyme. To unravel the physiological significance of these two enzymes, we analyzed their subcellular localization and detailed biochemical properties. Sucrose gra ...
PubMed:
11080311
PubMed Central:
PMC59233

4. Complex I impairment, respiratory compensations, and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris

Author:
Sabar, M.; Paepe, R. de.; Kouchkovsky, Y. de.
Source:
Plant physiology 2000 v.124 no.3 pp. 1239-1249
ISSN:
0032-0889
Subject:
Nicotiana sylvestris; leaves; mitochondria; photosynthesis; malic acid; succinic acid; pyruvic acid; oxidation; rotenone; oxidoreductases; enzyme activity; genetic code; genes; gene expression; cytoplasmic male sterility; mutants; genetic variation; glycine (amino acid); genotype; NAD (coenzyme); NADP (coenzyme)
Abstract:
... We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Complex I dysfunction. In contrast, the oxidation rate of exogenous NADH and the ca ...
PubMed:
11080300
PubMed Central:
PMC59222

5. Dehydration-induced redistribution of amphiphilic molecules between cytoplasm and lipids is associated with desiccation tolerance in seeds

Author:
Buitink, J.; Leprince, O.; Hoekstra, F.A.
Source:
Plant physiology 2000 v.124 no.3 pp. 1413-1425
ISSN:
0032-0889
Subject:
Pisum sativum; Cucumis sativus; seeds; imbibition; lipids; cytoplasm; viscosity; measurement; mathematical models; equations; translocation (plant physiology); stress response
Abstract:
... This study establishes a relationship between desiccation tolerance and the transfer of amphiphilic molecules from the cytoplasm into lipids during drying, using electron paramagnetic resonance spectroscopy of amphiphilic spin probes introduced into imbibed radicles of pea (Pisum sativum) and cucumber (Cucumis sativa) seeds. Survival following drying and a membrane integrity assay indicated that d ...
PubMed:
11080316
PubMed Central:
PMC59238

6. Hydroxylated phytosiderophore species possess an enhanced chelate stability and affinity for iron(III)

Author:
Wiren, N. von.; Khodr, H.; Hider, R.C.
Source:
Plant physiology 2000 v.124 no.3 pp. 1149-1157
ISSN:
0032-0889
Subject:
iron; ion transport; Poaceae; pH; structure-activity relationships; siderophores; chelation; roots
Abstract:
... Graminaceous plant species acquire soil iron by the release of phytosiderophores and subsequent uptake of iron(III)-phytosiderophore complexes. As plant species differ in their ability for phytosiderophore hydroxylation prior to release, an electrophoretic method was set up to determine whether hydroxylation affects the net charge of iron(III)-phytosiderophore complexes, and thus chelate stability ...
PubMed:
11080292
PubMed Central:
PMC59214

7. Impact of post-anoxia stress on membrane lipids of anoxia-pretreated potato cells. A re-appraisal

Author:
Pavelic, D.; Arpagaus, S.; Rawyler, A.; Brandle, R.
Source:
Plant physiology 2000 v.124 no.3 pp. 1285-1292
ISSN:
0032-0889
Subject:
anaerobic conditions; gas exchange; lipid peroxidation; linoleate 13S-lipoxygenase; Solanum tuberosum; stress response; lipids; biochemical pathways; cultured cells; cell membranes; hydrolysis; chemical constituents of plants; enzyme activity; oxygen
Abstract:
... The importance of lipid peroxidation and its contributing pathways (via reactive oxygen species and lipoxygenase) during post-anoxia was evaluated with respect to the biphasic behavior of membrane lipids under anoxia (A. Rawyler, D. Pavelic, C. Gianinazzi, J. Oberson, R. Brandle [1999] Plant Physiol 120: 293-300), using potato (Solanum tuberosum cv Bintje) cell cultures. When anoxic cells in the p ...
PubMed:
11080304
PubMed Central:
PMC59226

8. Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements

Author:
Liu, K.; Fu, H.; Bei, Q.; Luan, S.
Source:
Plant physiology 2000 v.124 no.3 pp. 1315-1325
ISSN:
0032-0889
Subject:
Vicia faba; seedlings; leaves; guard cells; stomatal movement; potassium; spermidine; spermine; cadaverine; putrescine; dose response; membrane potential; plasma membrane; ion exchange
Abstract:
... A number of studies show that environmental stress conditions such as drought, high salt, and air pollutants increase polyamine levels in plant cells. However, little is understood about the physiological function of elevated polyamine levels.We report here that polyamines regulate the voltage-dependent inward K+ channel in the plasma membrane of guard cells and modulate stomatal aperture, a plant ...
PubMed:
11080307
PubMed Central:
PMC59229

9. Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae

Author:
Sharples, J.M.; Meharg, A.A.; Chambers, S.M.; Cairney, J.W.G.
Source:
Plant physiology 2000 v.124 no.3 pp. 1327-1334
ISSN:
0032-0889
Subject:
Calluna vulgaris; Ascomycota; mycorrhizal fungi; symbiosis; arsenates; phytotoxicity; ion transport; quantitative analysis; mine spoil; chemical constituents of plants; heathlands; United Kingdom
Abstract:
... Arsenate resistance is exhibited by the ericoid mycorrhizal fungus Hymenoscyphus ericae collected from As-contaminated mine soils. To investigate the mechanism of arsenate resistance, uptake kinetics for arsenate (H2AsO4-), arsenite (H3AsO3), and phosphate (H2PO4-) were determined in both arsenate-resistant and -non-resistant H. ericae. The uptake kinetics of H2AsO4-, H3AsO3, and H2PO4- in both re ...
PubMed:
11080308
PubMed Central:
PMC59230

10. Modular domain structure of Arabidopsis COP1. Reconstitution of activity by fragment complementation and mutational analysis of a nuclear localization signal in planta

Author:
Stacey, M.G.; Kopp, O.R.; Kim, T.H.; Von Arnim, A.G.
Source:
Plant physiology 2000 v.124 no.3 pp. 979-989
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; plant development; alleles; phenotype; morphogenesis; plant anatomy; transgenic plants; Agrobacterium radiobacter; developmental stages; gene expression; mutants; genetic variation; enzyme activity; plant morphology; beta-glucuronidase; light
Abstract:
... The Arabidopsis COP1 protein functions as a developmental regulator, in part by repressing photomorphogenesis in darkness. Using complementation of a cop1 loss-of-function allele with transgenes expressing fusions of cop1 mutant proteins and beta-glucuronidase, it was confirmed that COP1 consists of two modules, an amino terminal module conferring a basal function during development and a carboxyl ...
PubMed:
11080276
PubMed Central:
PMC59198

11. Overexpression of auxin-binding protein enhances the sensitivity of guard cells to auxin

Author:
Bauly, J.M.; Sealy, I.M.; Macdonald, H.; Brearley, J.; Droge, S.; Hillmer, S.; Robinson, D.G.; Venis, M.A.; Blatt, M.R.; Lazarus, C.M.
Source:
Plant physiology 2000 v.124 no.3 pp. 1229-1238
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Zea mays; guard cells; potassium; auxins; binding proteins; transgenic plants; gene expression; mutants; genetic variation; developmental stages; histochemistry; genotype; ion exchange
Abstract:
... To explore the role of auxin-binding protein (ABP1) in planta, a number of transgenic tobacco (Nicotiana tabacum) lines were generated. The wild-type KDEL endoplasmic reticulum targeting signal was mutated to HDEL, another common retention sequence in plants, and to KEQL or KDELGL to compromise its activity. The auxin-binding kinetics of these forms of ABP1 were found to be similar to those of ABP ...
PubMed:
11080299
PubMed Central:
PMC59221

12. Production of recombinant proteins in tobacco guttation fluid

Author:
Komarnytsky, S.; Borisjuk, N.V.; Borisjuk, L.G.; Alam, M.Z.; Raskin, I.
Source:
Plant physiology 2000 v.124 no.3 pp. 927-933
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; intercellular spaces; guttation; alkaline phosphatase; recombinant proteins; protein synthesis; genes; gene transfer; transgenic plants; gene expression; diurnal variation; temperature; chemical constituents of plants; green fluorescent protein; plant secretions and exudates
Abstract:
... Guttation, the loss of water and dissolved materials from uninjured plant organs, is a common phenomenon in higher plants. By using endoplasmic reticulum signal peptides fused to the recombinant protein sequences, we have generated transgenic tobacco (Nicotiana tabacum L. cv Wisconsin) plants that secrete three heterologous proteins of different genetic backgrounds (bacterial xylanase, green fluor ...
PubMed:
11080270
PubMed Central:
PMC1539288

13. Sensing of osmotic pressure changes in tomato cells

Author:
Felix, G.; Regenass, M.; Boller, T.
Source:
Plant physiology 2000 v.124 no.3 pp. 1169-1179
ISSN:
0032-0889
Subject:
1-aminocyclopropane-1-carboxylic acid; Solanum lycopersicum var. lycopersicum; cell suspension culture; osmotic pressure; culture media; alkalinity; potassium; ethylene; biosynthesis; ligases; enzyme activity; phosphorylation; ion exchange; stress response
Abstract:
... Cells of tomato (Lycopersicon esculentum) growing in suspension gradually depleted their culture medium and caused a steady decrease in its osmolality. When confronted with a sudden change in medium osmolality (a hypo-osmotic or hyperosmotic shock), respectively, these cells responded with volume changes and stress symptoms such as rapid extracellular alkalinization, efflux of K+ -ions, and induct ...
PubMed:
11080294
PubMed Central:
PMC59216

14. Spatial distribution of cell division rate can be deduced from that of p34(cdc2) kinase activity in maize leaves grown at contrasting temperatures and soil water conditions

Author:
Granier, C.; Inze, D.; Tardieu, F.
Source:
Plant physiology 2000 v.124 no.3 pp. 1393-1402
ISSN:
0032-0889
Subject:
Zea mays; leaves; cell division; spatial distribution; developmental stages; enzyme activity; temperature; soil; water content; mathematical models; equations; chemical constituents of plants
Abstract:
... We have investigated the spatial distributions of cell division rate, p34cdc2 kinase activity, and amount of p34cdc2a in maize (Zea mays) leaves grown at contrasting temperatures and soil water conditions. An original method for calculating cell division rate in all leaf tissues is proposed. In all studied conditions, cell division rate was stable and maximum in the first 2 cm beyond the leaf inse ...
PubMed:
11080314
PubMed Central:
PMC59236

15. The involvement of a cysteine proteinase in the nodule development in Chinese milk vetch infected with Mesorhizobium huakuii subsp. rengei

Author:
Naito, Y.; Fujie, M.; Usami, S.; Murooka, Y.; Yamada, T.
Source:
Plant physiology 2000 v.124 no.3 pp. 1087-1095
ISSN:
0032-0889
Subject:
complementary DNA; symbiosis; root nodules; amino acid sequences; gene expression; genes; Astragalus sinicus; nodulation; enzyme activity; Rhizobiaceae; cysteine proteinases
Abstract:
... Cys proteinases play important roles in plant cell development and senescence. A cDNA, AsNODf32, obtained by differential screening of a nodule cDNA library ofthe leguminous plant Chinese milk vetch (Astragalus sinicus), represents a nodule-specific Cys proteinase similar to that reported for the actinorhizal Alnus glutinosa-Flankia symbiosis. A characteristic feature of this proteinase is the pre ...
PubMed:
11080286
PubMed Central:
PMC59208

16. Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in Arabidopsis mutants altered in phenolic metabolism

Author:
Booij-James, I.S.; Dube, S.K.; Jansen, M.A.K.; Edelman, M.; Mattoo, A.K.
Source:
Plant physiology 2000 v.124 no.3 pp. 1275-1283
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; biosynthesis; ecotypes; light harvesting complex; biochemical pathways; photosystem II; mutants; genetic variation; ultraviolet radiation; phenolic compounds
Abstract:
... Ultraviolet-B (UV-B) radiation can have a negative impact on the growth and development of plants. Plants tolerant to UV-B alleviate these effects using UV-screening pigments that reduce the penetration of UV-B into mesophyll tissue. Little is known about the relative contribution of specific phenolic compounds to the screening capacity of leaves. The D1 and D2 proteins constituting the photosyste ...
Handle:
10113/32980
PubMed:
11080303
PubMed Central:
PMC59225

17. A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize

Author:
McGonigle, B.; Keeler, S.J.; Lau, S.M.C.; Koeppe, M.K.; O'Keefe, D.P.
Source:
Plant physiology 2000 v.124 no.3 pp. 1105-1120
ISSN:
0032-0889
Subject:
Glycine max; Zea mays; glutathione transferase; enzyme activity; genes; gene expression; clones; genetic variation; chromosome mapping; amino acid sequences
Abstract:
... m1 BLAST searching a large expressed sequence tag database for glutathione S-transferase (GST) sequences we have identified 25 soybean (Glycine max) and 42 maize (Zea mays) clones and obtained accurate full-length GST sequences. These clones probably represent the majority of members of the GST multigene family in these species. Plant GSTs are divided according to sequence similarity into three ca ...
PubMed:
11080288
PubMed Central:
PMC59210

18. A leaf lipoxygenase of potato induced specifically by pathogen infection

Author:
Kolomiets, M.V.; Chen, H.; Gladon, R.J.; Braun, E.J.; Hannapel, D.J.
Source:
Plant physiology 2000 v.124 no.3 pp. 1121-1130
ISSN:
0032-0889
Subject:
genetic resistance; linoleate 13S-lipoxygenase; Solanum tuberosum; gene expression; plant pathogenic fungi; disease resistance; Phytophthora nicotianae var. parasitica; genes; enzyme activity; leaves; defense mechanisms
Abstract:
... Lipoxygenase (LOX) activity has been identified consistently during pathogen-induced defense responses. Here we report the involvement of a specific leaf LOX gene of potato (Solanum tuberosum), designated POTLX-3 (GenBank/EMBL accession no. U60202), in defense responses against pathogens. The sequence of POTLX-3 does not match any other LOX genes of potato and has the greatest match to a tobacco L ...
PubMed:
11080289
PubMed Central:
PMC59211

19. Aluminum-induced 1 leads to 3-beta-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata. A new mechanism of aluminum toxicity in plants

Author:
Sivaguru, M.; Fujiwara, T.; Samaj, J.; Baluska, F.; Yang, Z.; Osawa, H.; Maeda, T.; Mori, T.; Volkmann, D.; Matsumoto, H.
Source:
Plant physiology 2000 v.124 no.3 pp. 991-1005
ISSN:
0032-0889
Subject:
Triticum aestivum; roots; aluminum; phytotoxicity; plasmodesmata; glucans; callose; immunohistochemistry; developmental stages; translocation (plant physiology); symplast; stress response
Abstract:
... Symplastic intercellular transport in plants is achieved by plasmodesmata (PD). These cytoplasmic channels are well known to interconnect plant cells to facilitate intercellular movement of water, nutrients, and signaling molecules including hormones. However, it is not known whether Al may affect this cell-to-cell transport process, which is a critical feature for roots as organs of nutrient/wate ...

20. Analysis of the alternative pathways for the beta-oxidation of unsaturated fatty acids using transgenic plants synthesizing polyhydroxyalkanoates in peroxisomes

Author:
Allenbach, L.; Poirier, Y.
Source:
Plant physiology 2000 v.124 no.3 pp. 1159-1168
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; unsaturated fatty acids; oxidation; oxidoreductases; isomerases; enzyme activity; biochemical pathways; transgenic plants; gene transfer; Pseudomonas aeruginosa; genes; gene expression
Abstract:
... Degradation of fatty acids having cis-double bonds on even-numbered carbons requires the presence of auxiliary enzymes in addition to the enzymes of the core beta-oxidation cycle. Two alternative pathways have been described to degrade these fatty acids. One pathway involves the participation of the enzymes 2,4-dienoyl-coenzyme A (CoA) reductase and delta3-delta2-enoyl-CoA isomerase, whereas the s ...
PubMed:
11080293
PubMed Central:
PMC59215