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- Author:
- Klusener, B.; Weiler, E.W.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1399-1405
- ISSN:
- 0032-0889
- Subject:
- calcium chloride; root tips; calcium; ion transport; cations; cell membranes; Bryonia cretica; Lepidium sativum; endoplasmic reticulum; inorganic ions
- Abstract:
- ... A Ca(2+) channel from root-tip endomembranes of garden cress (Lepidium sativum L.) (LCC1) was characterized using the planar lipid-bilayer technique. Investigation of single-channel recordings revealed that LCC1 is voltage gated and strongly rectifying. In symmetrical 50 mM CaCl(2) solutions, the single-channel conductance was 24 picosiemens. LCC1 showed a moderate selectivity for Ca(2+) over K(+) ...
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- Author:
- Strand, A.; Hurry, V.; Henkes, S.; Huner, N.; Gustafsson, P.; Gardestrom, P.; Stitt, M.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1387-1397
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant development; volume; Calvin cycle; enzymes; sucrose; air temperature; acclimation; starch; cytoplasm; phosphates; cold; leaves
- Abstract:
- ... Photosynthetic and metabolic acclimation to low growth temperatures were studied in Arabidopsis (Heynh.). Plants were grown at 23 degrees C and then shifted to 5 degrees C. We compared the leaves shifted to 5 degrees C for 10 d and the new leaves developed at 5 degrees C with the control leaves on plants that had been left at 23 degrees C. Leaf development at 5 degrees C resulted in the recovery o ...
- PubMed:
- 10198098
- PubMed Central:
- PMC32024
-
- Author:
- Artus, N.N.; Nadler, K.D.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1177-1186
- ISSN:
- 0032-0889
- Subject:
- computer-assisted instruction; educational materials; college students; plant physiology
- Abstract:
- ... We used Computer-Assisted Personalized Approach (CAPA), a networked teaching and learning tool that generates computer individualized homework problem sets, in our large-enrollment introductory plant physiology course. We saw significant improvement in student examination performance with regular homework assignments, with CAPA being an effective and efficient substitute for hand-graded homework. ...
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- Author:
- Renard, C.M.G.C.; Jarvis, M.C.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1315-1322
- ISSN:
- 0032-0889
- Subject:
- cell wall components; hemicellulose; nuclear magnetic resonance spectroscopy; pectins; cell walls; arabinose; cellulose; polymers; galacturonans; Beta vulgaris
- Abstract:
- ... Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T(1p) decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of approximately 3 nm, mobile pectic galacturonans, and highly mobile arabinans. A d ...
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- Author:
- Damme, E.J.M. van; Charels, D.; Roy, S.; Tierens, K.; Barre, A.; Martins, J.C.; Rouge, P.; Leuven, F. van.; Does, M.; Peumans, W.J.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1547-1556
- ISSN:
- 0032-0889
- Subject:
- taxonomy; Sambucus nigra; amino acid sequences; genes; nucleotide sequences; chitinase
- Abstract:
- ... We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that ...
- PubMed:
- 10198114
- PubMed Central:
- PMC32040
-
- Author:
- Sunako, T.; Sakuraba, W.; Senda, M.; Akada, S.; Ishikawa, R.; Niizeki, M.; Harada, T.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1297-1303
- ISSN:
- 0032-0889
- Subject:
- cultivars; 1-aminocyclopropane-1-carboxylic acid; shelf life; apples; alleles; gases; messenger RNA; complementary DNA; polymerase chain reaction; heterozygosity; amino acid sequences; ethylene production; transcription (genetics); ripening; nucleotide sequences; Malus domestica; homozygosity
- Abstract:
- ... An allele of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (Md-ACS1), the transcript and translated product of which have been identified in ripening apples (Malus domestica), was isolated from a genomic library of the apple cultivar, Golden Delicious. The predicted coding region of this allele (ACS1-2) showed that seven nucleotide substitutions in the corresponding region of ACS1- ...
- PubMed:
- 10198088
- PubMed Central:
- PMC32014
-
- Author:
- Piao, H.L.; Pih, K.T.; Lim, J.H.; Kang, S.G.; Jin, J.B.; Kim, S.H.; Hwang, I.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1527-1534
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; Saccharomyces cerevisiae; mutants; genes; salinity; sodium chloride; abscisic acid; gene expression; complementary DNA; amino acid sequences; nucleotide sequences; potassium chloride; genetic complementation; phenotype; biological resistance
- Abstract:
- ... GSK3/shaggy-like genes encode kinases that are involved in a variety of biological processes. By functionalcomplementation of the yeast calcineurin mutant strain DHT22-1a with a NaCl stress-sensitive phenotype, we isolated the Arabidopsis cDNA AtGSK1, which encodes a GSK3/shaggy-like protein kinase. AtGSK1 rescued the yeast calcineurin mutant cells from the effects of high NaCl. Also, the AtGSK1 g ...
- PubMed:
- 10198112
- PubMed Central:
- PMC32038
-
- Author:
- Yu, X.M.; Griffith, M.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1361-1369
- ISSN:
- 0032-0889
- Subject:
- freezing; polypeptides; winter; immunochemistry; cell walls; acclimation; Secale cereale; beta-glucanase; chitinase; surfaces; crystallization; ice; pathogenesis; enzyme activity; cold tolerance; chemical structure; leaves
- Abstract:
- ... Antifreeze proteins (AFPs) similar to three pathogenesis-related proteins, a glucanase-like protein (GLP), a chitinase-like protein (CLP), and a thaumatin-like protein (TLP), accumulate during cold acclimation in winter rye (Secale cereale) leaves, where they are thought to modify the growth of intercellular ice during freezing. The objective of this study was to characterize the rye AFPs in their ...
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- Author:
- Movafeghi, A.; Happel, N.; Pimpl, P.; Tai, G.H.; Robinson, D.G.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1437-1445
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; Brassica oleracea var. botrytis; plant proteins; protein transport; endoplasmic reticulum; Golgi apparatus; binding proteins; guanosine triphosphate; inflorescences; immunocytochemistry; cytosol; cell membranes
- Abstract:
- ... Intracellular protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus and within the Golgi apparatus is facilitated by COP (coat protein)-coated vesicles. Their existence in plant cells has not yet been demonstrated, although the GTP-binding proteins required for coat formation have been identified. We have generated antisera against glutathione-S-transferase-fusion protei ...
- PubMed:
- 10198103
- PubMed Central:
- PMC32029
-
- Author:
- Zentella, R.; Mascorro-Gallardo, J.O.; Dijck, P. van.; Folch-Mallol, J.; Bonini, B.; Vaeck, C. van.; Gaxiola, R.; Covarrubias, A.A.; Nieto-Sotelo, J.; Thevelein, J.M.; Iturriaga, G.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1473-1482
- ISSN:
- 0032-0889
- Subject:
- Saccharomyces cerevisiae; hexosyltransferases; mutants; complementary DNA; molecular weight; plant proteins; amino acid sequences; nucleotide sequences; protein composition; messenger RNA; gene expression; genetic transformation; heat treatment; culture media; heat tolerance; sodium chloride; genetic complementation; phenotype
- Abstract:
- ... The accumulation ofthe disaccharide trehalose in anhydrobiotic organisms allows them to survive severe environmental stress. A plant cDNA, SlTPS1, encoding a 109-kD protein, was isolated from the resurrection plant Selaginella lepidophylla, which accumulates high levels of trehalose. Protein-sequence comparison showed that SlTPS1 shares high similarity to trehalose-6-phosphate synthase genes from ...
- PubMed:
- 10198107
- PubMed Central:
- PMC32033
-
- Author:
- Nomura, T.; Kitasaka, Y.; Takatsuto, S.; Reid, J.B.; Fukami, M.; Yokota, T.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1517-1526
- ISSN:
- 0032-0889
- Subject:
- weight; dwarfing; biosynthesis; phenotype; brassinosteroids; plant characteristics; mutation; height; biochemical pathways; cell membranes; chemical constituents of plants; Pisum sativum; leaves; shoots
- Abstract:
- ... The dwarf pea (Pisum sativum) mutants lka and lkb are brassinosteroid (BR) insensitive and deficient, respectively. The dwarf phenotype of the lkb mutant was rescued to wild type by exogenous application of brassinolide and its biosynthetic precursors. Gas chromatography-mass spectrometry analysis of the endogenous sterols in this mutant revealed that it accumulates 24-methylenecholesterol and iso ...
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- Author:
- Heilmann, I.; Perera, I.Y.; Gross, W.; Boss, W.F.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1331-1339
- ISSN:
- 0032-0889
- Subject:
- weight; chemical constituents of plants; lipid metabolism; biochemical pathways; phosphatidylinositols; phospholipase C; cultured cells; duration; phosphotransferases (kinases); enzyme activity; Rhodophyta; metabolites
- Abstract:
- ... The metabolism of phosphatidylinositol-4,5-bisphosphate (PIP2) changed during the culture period of the thermoacidophilic red alga Galdieria sulphuraria. Seven days after inoculation, the amount of PIP(2) in the cells was 910 +/- 100 pmol g(-1) fresh weight; by 12 d, PIP(2) levels increased to 1200 +/- 150 pmol g(-1) fresh weight. In vitro assays indicated that phosphatidylinositol monophosphate ( ...
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- Author:
- Garcia, I.; Rodgers, M.; Pepin, R.; Hsieh, T.F.; Matringe, M.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1507-1516
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant tissues; biosynthesis; complementary DNA; quinones; transgenic plants; recombinant proteins; cytosol; tyrosine; gene expression; amino acid sequences; Nicotiana tabacum; nucleotide sequences; genetic techniques and protocols; leaves; enzyme activity; immunocytochemistry; oxygenases; chloroplasts
- Abstract:
- ... 4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We report here the molecular and biochemical characterization of an Arabidopsis 4HPPD an ...
- PubMed:
- 10198110
- PubMed Central:
- PMC32036
-
- Author:
- Fleischmann, M.M.; Rochaix, J.D.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1557-1566
- ISSN:
- 0032-0889
- Subject:
- polypeptides; threonine; valine; phenotype; aspartic acid; fluorescence; glycine (amino acid); alanine; photoinhibition; amino acid sequences; photosystem II; mutants; glutamic acid; Chlamydomonas reinhardtii; phosphorylation; protein metabolism; protein composition
- Abstract:
- ... We have changed the potential phosphorylation site, a threonine residue at position 2 of the D2 polypeptide of the photosystem II complex of Chlamydomonas reinhardtii, to alanine, valine, aspartate, proline, glycine, or glutamate. Mutants with neutral amino acid changes did not display any phenotype with regard to photoautotrophic growth, light sensitivity, fluorescence transients, or photoinhibit ...
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- Author:
- Llop-Tous, I.; Dominguez-Puigjaner, E.; Palomer, X.; Vendrell, M.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1415-1421
- ISSN:
- 0032-0889
- Subject:
- plant development; messenger RNA; complementary DNA; Fragaria ananassa; cellulases; phylogeny; peptides; amino acid sequences; gene expression; firmness; ripening; nucleotide sequences; molecular weight; isozymes; molecular conformation; plant proteins
- Abstract:
- ... Two cDNAs clones (Cel1 and Cel2) encoding divergent endo-beta-1,4-glucanases (EGases) have been isolated from a cDNA library obtained from ripe strawberry (Fragaria x ananassa Duch) fruit. The analysis of the amino acid sequence suggests that Cel1 and Cel2 EGases have different secondary and tertiary structures and that they differ in the presence of potential N-glycosylation sites. By in vitro tr ...
- PubMed:
- 10198101
- PubMed Central:
- PMC32027
-
- Author:
- Vivanco, J.M.; Savary, B.J.; Flores, H.E.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1447-1456
- ISSN:
- 0032-0889
- Subject:
- Mirabilis; ribosomes; roots; tubers; molecular weight; isoelectric point; pH; amino acid sequences; protein composition; molecular conformation; antifungal properties; antibacterial properties; ribosomal RNA; hyphae; plant pathogenic bacteria; plant pathogenic fungi; antiviral properties
- Abstract:
- ... Two novel type I ribosome-inactivating proteins (RIPs) were found in the storage roots of Mirabilis expansa, an underutilized Andean root crop. The two RIPs, named ME1 and ME2, were purified to homogeneity by ammonium sulfate precipitation, cation-exchange perfusion chromatography, and C(4) reverse-phase chromatography. The two proteins were found to be similar in size (27 and 27.5 kD) by sodium d ...
- PubMed:
- 10198104
- PubMed Central:
- PMC32030
-
- Author:
- Weaver, L.M.; Froehlich, J.E.; Amasino, R.M.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1209-1216
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; messenger RNA; protein synthesis; biomarkers; proteinases; amino acid sequences; protein transport; leaves; chloroplasts; plant proteins
- Abstract:
- ... Arabidopsis ERD1 is a ClpC-like protein that sequence analysis suggests may interact with the chloroplast-localized ClpP protease to facilitate proteolysis. The mRNA encoded by the ERD1 gene has previously been shown to accumulate in response to senescence and to avariety of stresses and hormones. Here we show that the ERD1 protein, in contrast to the ERD1 mRNA, strongly declines in abundance with ...
- PubMed:
- 10198079
- PubMed Central:
- PMC32005
-
- Author:
- Rogers, S.W.; Rogers, J.C.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1457-1464
- ISSN:
- 0032-0889
- Subject:
- Hordeum vulgare; ribonucleases; gibberellins; aleurone layer; gene expression; complementary DNA; leaves; amino acid sequences; nucleotide sequences; Nicotiana tabacum; transgenic plants; cell suspension culture; protoplasts; enzyme activity; abscisic acid; cycloheximide; alpha-amylase
- Abstract:
- ... We cloned a cDNA for a gibberellin-induced ribonuclease (RNase) expressed in barely (Hordeum vulgare) aleurone and the gene for a second barley RNase expressed in leaf tissue. The protein encoded by the cDNA is unique among RNases described to date in that it contains a novel 23-amino acid insert between the C2 and C3 conserved sequences. Expression of the recombinant protein in tobacco (Nicotiana ...
- PubMed:
- 10198105
- PubMed Central:
- PMC32031
-
- Author:
- Cercos, M.; Santamaria, S.; Carbonell, J.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1341-1348
- ISSN:
- 0032-0889
- Subject:
- seed germination; messenger RNA; gynoecium; complementary DNA; proteinases; gibberellic acid; amino acid sequences; gene expression; genes; nucleotide sequences; Pisum sativum
- Abstract:
- ... A cDNA clone encoding a thiol-protease (TPE4A) was isolated from senescent ovaries of pea (Pisum sativum) by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of TPE4A has the conserved catalytic amino acids of papain. It is very similar to VSCYSPROA, a thiol-protease induced during seed germination in common vetch. TPE4A mRNA levels increase during the senescence of ...
- PubMed:
- 10198093
- PubMed Central:
- PMC32019
-
- Author:
- Matsui, K.; Hijiya, K.; Tabuchi, Y.; Kajiwara, T.
- Source:
- Plant physiology 1999 v.119 no.4 pp. 1279-1287
- ISSN:
- 0032-0889
- Subject:
- seed germination; seedling growth; messenger RNA; complementary DNA; Cucumis sativus; linoleate 13S-lipoxygenase; lipid metabolism; lipid bodies; cotyledons; amino acid sequences; gene expression; cytosol; genes; nucleotide sequences; enzyme activity; isocitrate lyase
- Abstract:
- ... In cucumber (Cucumis sativus), high lipoxygenas-1 (LOX-1) activity has been detected in the soluble fraction prepared from cotyledons of germinating seeds, and the involvement of this enzyme in lipid turnover has been suggested (K. Matsui, M. Irie, T. Kajiwara, A. Hatanaka [1992] Plant Sci 85: 23-32; I. Fuessner, C. Wasternack, H. Kindl, H. Kuhn [1995] Proc Natl Acad Sci USA 92: 11849-11853). In t ...
- PubMed:
- 10198086
- PubMed Central:
- PMC32012
-