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You searched for: Journal Plant physiology Remove constraint Journal: Plant physiology Publication Year 1999 Remove constraint Publication Year: 1999 Source 1999 v.119 no.4 Remove constraint Source: 1999 v.119 no.4
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1. A calcium-selective channel from root-tip endomembranes of garden cress

Author:
Klusener, B.; Weiler, E.W.
Source:
Plant physiology 1999 v.119 no.4 pp. 1399-1405
ISSN:
0032-0889
Subject:
calcium chloride; root tips; calcium; ion transport; cations; cell membranes; Bryonia cretica; Lepidium sativum; endoplasmic reticulum; inorganic ions
Abstract:
... A Ca(2+) channel from root-tip endomembranes of garden cress (Lepidium sativum L.) (LCC1) was characterized using the planar lipid-bilayer technique. Investigation of single-channel recordings revealed that LCC1 is voltage gated and strongly rectifying. In symmetrical 50 mM CaCl(2) solutions, the single-channel conductance was 24 picosiemens. LCC1 showed a moderate selectivity for Ca(2+) over K(+) ...

2. Acclimation of Arabidopsis leaves developing at low temperatures. Increasing cytoplasmic volume accompanies increased activities of enzymes in the Calvin cycle and in the sucrose-biosynthesis pathway

Author:
Strand, A.; Hurry, V.; Henkes, S.; Huner, N.; Gustafsson, P.; Gardestrom, P.; Stitt, M.
Source:
Plant physiology 1999 v.119 no.4 pp. 1387-1397
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant development; volume; Calvin cycle; enzymes; sucrose; air temperature; acclimation; starch; cytoplasm; phosphates; cold; leaves
Abstract:
... Photosynthetic and metabolic acclimation to low growth temperatures were studied in Arabidopsis (Heynh.). Plants were grown at 23 degrees C and then shifted to 5 degrees C. We compared the leaves shifted to 5 degrees C for 10 d and the new leaves developed at 5 degrees C with the control leaves on plants that had been left at 23 degrees C. Leaf development at 5 degrees C resulted in the recovery o ...
PubMed:
10198098
PubMed Central:
PMC32024

3. A computer-assisted personalized approach in an undergraduate plant physiology class

Author:
Artus, N.N.; Nadler, K.D.
Source:
Plant physiology 1999 v.119 no.4 pp. 1177-1186
ISSN:
0032-0889
Subject:
computer-assisted instruction; educational materials; college students; plant physiology
Abstract:
... We used Computer-Assisted Personalized Approach (CAPA), a networked teaching and learning tool that generates computer individualized homework problem sets, in our large-enrollment introductory plant physiology course. We saw significant improvement in student examination performance with regular homework assignments, with CAPA being an effective and efficient substitute for hand-graded homework. ...

4. A cross-polarization, magic-angle-spinning, 13C-nuclear-magnetic-resonance study of polysaccharides in sugar beet cell walls

Author:
Renard, C.M.G.C.; Jarvis, M.C.
Source:
Plant physiology 1999 v.119 no.4 pp. 1315-1322
ISSN:
0032-0889
Subject:
cell wall components; hemicellulose; nuclear magnetic resonance spectroscopy; pectins; cell walls; arabinose; cellulose; polymers; galacturonans; Beta vulgaris
Abstract:
... Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T(1p) decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of approximately 3 nm, mobile pectic galacturonans, and highly mobile arabinans. A d ...

5. A gene encoding a Hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes

Author:
Damme, E.J.M. van; Charels, D.; Roy, S.; Tierens, K.; Barre, A.; Martins, J.C.; Rouge, P.; Leuven, F. van.; Does, M.; Peumans, W.J.
Source:
Plant physiology 1999 v.119 no.4 pp. 1547-1556
ISSN:
0032-0889
Subject:
taxonomy; Sambucus nigra; amino acid sequences; genes; nucleotide sequences; chitinase
Abstract:
... We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that ...
PubMed:
10198114
PubMed Central:
PMC32040

6. An allele of the ripening-specific 1-aminocyclopropane-1-carboxylic acid synthase gene (ACS1) in apple fruit with a long storage life

Author:
Sunako, T.; Sakuraba, W.; Senda, M.; Akada, S.; Ishikawa, R.; Niizeki, M.; Harada, T.
Source:
Plant physiology 1999 v.119 no.4 pp. 1297-1303
ISSN:
0032-0889
Subject:
cultivars; 1-aminocyclopropane-1-carboxylic acid; shelf life; apples; alleles; gases; messenger RNA; complementary DNA; polymerase chain reaction; heterozygosity; amino acid sequences; ethylene production; transcription (genetics); ripening; nucleotide sequences; Malus domestica; homozygosity
Abstract:
... An allele of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (Md-ACS1), the transcript and translated product of which have been identified in ripening apples (Malus domestica), was isolated from a genomic library of the apple cultivar, Golden Delicious. The predicted coding region of this allele (ACS1-2) showed that seven nucleotide substitutions in the corresponding region of ACS1- ...
PubMed:
10198088
PubMed Central:
PMC32014

7. An Arabidopsis GSK3/shaggy-like gene that complements yeast salt stress-sensitive mutants is induced by NaCl and abscisic acid

Author:
Piao, H.L.; Pih, K.T.; Lim, J.H.; Kang, S.G.; Jin, J.B.; Kim, S.H.; Hwang, I.
Source:
Plant physiology 1999 v.119 no.4 pp. 1527-1534
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Saccharomyces cerevisiae; mutants; genes; salinity; sodium chloride; abscisic acid; gene expression; complementary DNA; amino acid sequences; nucleotide sequences; potassium chloride; genetic complementation; phenotype; biological resistance
Abstract:
... GSK3/shaggy-like genes encode kinases that are involved in a variety of biological processes. By functionalcomplementation of the yeast calcineurin mutant strain DHT22-1a with a NaCl stress-sensitive phenotype, we isolated the Arabidopsis cDNA AtGSK1, which encodes a GSK3/shaggy-like protein kinase. AtGSK1 rescued the yeast calcineurin mutant cells from the effects of high NaCl. Also, the AtGSK1 g ...
PubMed:
10198112
PubMed Central:
PMC32038

8. Antifreeze proteins in winter rye leaves form oligomeric complexes

Author:
Yu, X.M.; Griffith, M.
Source:
Plant physiology 1999 v.119 no.4 pp. 1361-1369
ISSN:
0032-0889
Subject:
freezing; polypeptides; winter; immunochemistry; cell walls; acclimation; Secale cereale; beta-glucanase; chitinase; surfaces; crystallization; ice; pathogenesis; enzyme activity; cold tolerance; chemical structure; leaves
Abstract:
... Antifreeze proteins (AFPs) similar to three pathogenesis-related proteins, a glucanase-like protein (GLP), a chitinase-like protein (CLP), and a thaumatin-like protein (TLP), accumulate during cold acclimation in winter rye (Secale cereale) leaves, where they are thought to modify the growth of intercellular ice during freezing. The objective of this study was to characterize the rye AFPs in their ...

9. Arabidopsis Sec21p and Sec23p homologs. Probable coat proteins of plant COP-coated vesicles

Author:
Movafeghi, A.; Happel, N.; Pimpl, P.; Tai, G.H.; Robinson, D.G.
Source:
Plant physiology 1999 v.119 no.4 pp. 1437-1445
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Brassica oleracea var. botrytis; plant proteins; protein transport; endoplasmic reticulum; Golgi apparatus; binding proteins; guanosine triphosphate; inflorescences; immunocytochemistry; cytosol; cell membranes
Abstract:
... Intracellular protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus and within the Golgi apparatus is facilitated by COP (coat protein)-coated vesicles. Their existence in plant cells has not yet been demonstrated, although the GTP-binding proteins required for coat formation have been identified. We have generated antisera against glutathione-S-transferase-fusion protei ...
PubMed:
10198103
PubMed Central:
PMC32029

10. A Selaginella lepidophylla trehalose-6-phosphate synthase complements growth and stress-tolerance defects in a yeast tps1 mutant

Author:
Zentella, R.; Mascorro-Gallardo, J.O.; Dijck, P. van.; Folch-Mallol, J.; Bonini, B.; Vaeck, C. van.; Gaxiola, R.; Covarrubias, A.A.; Nieto-Sotelo, J.; Thevelein, J.M.; Iturriaga, G.
Source:
Plant physiology 1999 v.119 no.4 pp. 1473-1482
ISSN:
0032-0889
Subject:
Saccharomyces cerevisiae; hexosyltransferases; mutants; complementary DNA; molecular weight; plant proteins; amino acid sequences; nucleotide sequences; protein composition; messenger RNA; gene expression; genetic transformation; heat treatment; culture media; heat tolerance; sodium chloride; genetic complementation; phenotype
Abstract:
... The accumulation ofthe disaccharide trehalose in anhydrobiotic organisms allows them to survive severe environmental stress. A plant cDNA, SlTPS1, encoding a 109-kD protein, was isolated from the resurrection plant Selaginella lepidophylla, which accumulates high levels of trehalose. Protein-sequence comparison showed that SlTPS1 shares high similarity to trehalose-6-phosphate synthase genes from ...
PubMed:
10198107
PubMed Central:
PMC32033

11. Brassinosteroid/sterol synthesis and plant growth as affected by lka and lkb mutations of pea

Author:
Nomura, T.; Kitasaka, Y.; Takatsuto, S.; Reid, J.B.; Fukami, M.; Yokota, T.
Source:
Plant physiology 1999 v.119 no.4 pp. 1517-1526
ISSN:
0032-0889
Subject:
weight; dwarfing; biosynthesis; phenotype; brassinosteroids; plant characteristics; mutation; height; biochemical pathways; cell membranes; chemical constituents of plants; Pisum sativum; leaves; shoots
Abstract:
... The dwarf pea (Pisum sativum) mutants lka and lkb are brassinosteroid (BR) insensitive and deficient, respectively. The dwarf phenotype of the lkb mutant was rescued to wild type by exogenous application of brassinolide and its biosynthetic precursors. Gas chromatography-mass spectrometry analysis of the endogenous sterols in this mutant revealed that it accumulates 24-methylenecholesterol and iso ...

12. Changes in phosphoinositide metabolism with days in culture affect signal transduction pathways in Galdieria sulphuraria

Author:
Heilmann, I.; Perera, I.Y.; Gross, W.; Boss, W.F.
Source:
Plant physiology 1999 v.119 no.4 pp. 1331-1339
ISSN:
0032-0889
Subject:
weight; chemical constituents of plants; lipid metabolism; biochemical pathways; phosphatidylinositols; phospholipase C; cultured cells; duration; phosphotransferases (kinases); enzyme activity; Rhodophyta; metabolites
Abstract:
... The metabolism of phosphatidylinositol-4,5-bisphosphate (PIP2) changed during the culture period of the thermoacidophilic red alga Galdieria sulphuraria. Seven days after inoculation, the amount of PIP(2) in the cells was 910 +/- 100 pmol g(-1) fresh weight; by 12 d, PIP(2) levels increased to 1200 +/- 150 pmol g(-1) fresh weight. In vitro assays indicated that phosphatidylinositol monophosphate ( ...

13. Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco

Author:
Garcia, I.; Rodgers, M.; Pepin, R.; Hsieh, T.F.; Matringe, M.
Source:
Plant physiology 1999 v.119 no.4 pp. 1507-1516
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant tissues; biosynthesis; complementary DNA; quinones; transgenic plants; recombinant proteins; cytosol; tyrosine; gene expression; amino acid sequences; Nicotiana tabacum; nucleotide sequences; genetic techniques and protocols; leaves; enzyme activity; immunocytochemistry; oxygenases; chloroplasts
Abstract:
... 4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We report here the molecular and biochemical characterization of an Arabidopsis 4HPPD an ...
PubMed:
10198110
PubMed Central:
PMC32036

14. Characterization of mutants with alterations of the phosphorylation site in the D2 photosystem II polypeptide of Chlamydomonas reinhardtii

Author:
Fleischmann, M.M.; Rochaix, J.D.
Source:
Plant physiology 1999 v.119 no.4 pp. 1557-1566
ISSN:
0032-0889
Subject:
polypeptides; threonine; valine; phenotype; aspartic acid; fluorescence; glycine (amino acid); alanine; photoinhibition; amino acid sequences; photosystem II; mutants; glutamic acid; Chlamydomonas reinhardtii; phosphorylation; protein metabolism; protein composition
Abstract:
... We have changed the potential phosphorylation site, a threonine residue at position 2 of the D2 polypeptide of the photosystem II complex of Chlamydomonas reinhardtii, to alanine, valine, aspartate, proline, glycine, or glutamate. Mutants with neutral amino acid changes did not display any phenotype with regard to photoautotrophic growth, light sensitivity, fluorescence transients, or photoinhibit ...

15. Characterization of two divergent endo-beta-1,4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit

Author:
Llop-Tous, I.; Dominguez-Puigjaner, E.; Palomer, X.; Vendrell, M.
Source:
Plant physiology 1999 v.119 no.4 pp. 1415-1421
ISSN:
0032-0889
Subject:
plant development; messenger RNA; complementary DNA; Fragaria ananassa; cellulases; phylogeny; peptides; amino acid sequences; gene expression; firmness; ripening; nucleotide sequences; molecular weight; isozymes; molecular conformation; plant proteins
Abstract:
... Two cDNAs clones (Cel1 and Cel2) encoding divergent endo-beta-1,4-glucanases (EGases) have been isolated from a cDNA library obtained from ripe strawberry (Fragaria x ananassa Duch) fruit. The analysis of the amino acid sequence suggests that Cel1 and Cel2 EGases have different secondary and tertiary structures and that they differ in the presence of potential N-glycosylation sites. By in vitro tr ...
PubMed:
10198101
PubMed Central:
PMC32027

16. Characterization of two novel type I ribosome-inactivating proteins from the storage roots of the Andean crop Mirabilis expansa

Author:
Vivanco, J.M.; Savary, B.J.; Flores, H.E.
Source:
Plant physiology 1999 v.119 no.4 pp. 1447-1456
ISSN:
0032-0889
Subject:
Mirabilis; ribosomes; roots; tubers; molecular weight; isoelectric point; pH; amino acid sequences; protein composition; molecular conformation; antifungal properties; antibacterial properties; ribosomal RNA; hyphae; plant pathogenic bacteria; plant pathogenic fungi; antiviral properties
Abstract:
... Two novel type I ribosome-inactivating proteins (RIPs) were found in the storage roots of Mirabilis expansa, an underutilized Andean root crop. The two RIPs, named ME1 and ME2, were purified to homogeneity by ammonium sulfate precipitation, cation-exchange perfusion chromatography, and C(4) reverse-phase chromatography. The two proteins were found to be similar in size (27 and 27.5 kD) by sodium d ...
PubMed:
10198104
PubMed Central:
PMC32030

17. Chloroplast-targeted ERD1 protein declines but its mRNA increases during senescence in Arabidopsis

Author:
Weaver, L.M.; Froehlich, J.E.; Amasino, R.M.
Source:
Plant physiology 1999 v.119 no.4 pp. 1209-1216
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; protein synthesis; biomarkers; proteinases; amino acid sequences; protein transport; leaves; chloroplasts; plant proteins
Abstract:
... Arabidopsis ERD1 is a ClpC-like protein that sequence analysis suggests may interact with the chloroplast-localized ClpP protease to facilitate proteolysis. The mRNA encoded by the ERD1 gene has previously been shown to accumulate in response to senescence and to avariety of stresses and hormones. Here we show that the ERD1 protein, in contrast to the ERD1 mRNA, strongly declines in abundance with ...
PubMed:
10198079
PubMed Central:
PMC32005

18. Cloning and characterization of a gibberellin-induced RNase expressed in barley aleurone cells

Author:
Rogers, S.W.; Rogers, J.C.
Source:
Plant physiology 1999 v.119 no.4 pp. 1457-1464
ISSN:
0032-0889
Subject:
Hordeum vulgare; ribonucleases; gibberellins; aleurone layer; gene expression; complementary DNA; leaves; amino acid sequences; nucleotide sequences; Nicotiana tabacum; transgenic plants; cell suspension culture; protoplasts; enzyme activity; abscisic acid; cycloheximide; alpha-amylase
Abstract:
... We cloned a cDNA for a gibberellin-induced ribonuclease (RNase) expressed in barely (Hordeum vulgare) aleurone and the gene for a second barley RNase expressed in leaf tissue. The protein encoded by the cDNA is unique among RNases described to date in that it contains a novel 23-amino acid insert between the C2 and C3 conserved sequences. Expression of the recombinant protein in tobacco (Nicotiana ...
PubMed:
10198105
PubMed Central:
PMC32031

19. Cloning and characterization of TPE4A, a thiol-protease gene induced during ovary senescence and seed germination in pea

Author:
Cercos, M.; Santamaria, S.; Carbonell, J.
Source:
Plant physiology 1999 v.119 no.4 pp. 1341-1348
ISSN:
0032-0889
Subject:
seed germination; messenger RNA; gynoecium; complementary DNA; proteinases; gibberellic acid; amino acid sequences; gene expression; genes; nucleotide sequences; Pisum sativum
Abstract:
... A cDNA clone encoding a thiol-protease (TPE4A) was isolated from senescent ovaries of pea (Pisum sativum) by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of TPE4A has the conserved catalytic amino acids of papain. It is very similar to VSCYSPROA, a thiol-protease induced during seed germination in common vetch. TPE4A mRNA levels increase during the senescence of ...
PubMed:
10198093
PubMed Central:
PMC32019

20. Cucumber cotyledon lipoxygenase during postgerminative growth. Its expression and action on lipid bodies

Author:
Matsui, K.; Hijiya, K.; Tabuchi, Y.; Kajiwara, T.
Source:
Plant physiology 1999 v.119 no.4 pp. 1279-1287
ISSN:
0032-0889
Subject:
seed germination; seedling growth; messenger RNA; complementary DNA; Cucumis sativus; linoleate 13S-lipoxygenase; lipid metabolism; lipid bodies; cotyledons; amino acid sequences; gene expression; cytosol; genes; nucleotide sequences; enzyme activity; isocitrate lyase
Abstract:
... In cucumber (Cucumis sativus), high lipoxygenas-1 (LOX-1) activity has been detected in the soluble fraction prepared from cotyledons of germinating seeds, and the involvement of this enzyme in lipid turnover has been suggested (K. Matsui, M. Irie, T. Kajiwara, A. Hatanaka [1992] Plant Sci 85: 23-32; I. Fuessner, C. Wasternack, H. Kindl, H. Kuhn [1995] Proc Natl Acad Sci USA 92: 11849-11853). In t ...
PubMed:
10198086
PubMed Central:
PMC32012