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You searched for: Journal name Plant physiology Remove constraint Journal name: Plant physiology Publication year rev 8003-1997 Remove constraint Publication year rev: 8003-1997 Source 1997 v.115 no.3 Remove constraint Source: 1997 v.115 no.3
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1. Apoplasmic and protoplasmic water transport through the parenchyma of the potato storage organ

Author:
Michael, W.; Schultz, A.; Meshcheryakov, A.B.; Ehwald, R.
Source:
Plant physiology 1997 v.115 no.3 pp. 1089-1099
ISSN:
0032-0889
Subject:
protoplasts; intercellular spaces; quantitative analysis; osmotic pressure; Solanum tuberosum; tubers; walls; hydraulic conductivity
Abstract:
... Stationary volume fluxes through living and denatured parenchyma slices of the potato (Solanum tuberosum L.) storage organ were studied to estimate the hydraulic conductivity of the cell wall and to evaluate the significance of water transport through protoplasts, cell walls, and intercellular spaces. Slices were placed between liquid compartments, steady-state fluxes induced by pressure or concen ...

2. The COW1 locus of Arabidopsis acts after RHD2, and in parallel with RHD3 and TIP1, to determine the shape, rate of elongation, and number of root hairs produced from each site of hair formation

Author:
Grierson, C.S.; Roberts, K.; Feldmann, K.A.; Dolan, L.
Source:
Plant physiology 1997 v.115 no.3 pp. 981-990
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; root hairs; morphogenesis; abnormal development; mutants; genetic variation; developmental stages; plant morphology; alleles; gene expression; phenotype; chromosome mapping
Abstract:
... Two recessive mutant alleles at CAN OF WORMS1 (COW1), a new locus involved in root hair morphogenesis, have been identified in Arabidopsis thaliana L. Heynh. Root hairs on Cow1- mutants are short and wide and occasionally formed as pairs at a single site of hair formation. The COW1 locus maps to chromosome 4. Root hairs on Cow1- plants form in the usual positions, suggesting that the phenotype is ...
PubMed:
9390433
PubMed Central:
PMC158561

3. Characterization of maize elongation factor 1A and its relationship to protein quality in the endosperm

Author:
Sun, Y.J.; Caneiro, N.; Clore, A.M.; Moro, G.L.; Habben, J.E.; Larkins, B.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 1101-1107
ISSN:
0032-0889
Subject:
lysine; Zea mays; protein synthesis; quantitative analysis; complementary DNA; binding proteins; clones; amino acid sequences; endosperm; genotype; mutants; nucleotide sequences; genetic variation; protein content
Abstract:
... The protein synthesis elongation factor 1A (eEF1A) is a multifunctional protein in eukaryotic cells. In maize (Zea mays L.) endosperm eEF1A co-localizes with actin around protein bodies, and its accumulation is highly correlated with the protein-bound lysine (Lys) content. We purified eEF1A from maize kernels by ammonium sulfate precipitation, ion-exchange, and chromatofocusing. The identity of th ...
PubMed:
9390440
PubMed Central:
PMC158574

4. Characterization of new gibberellin-responsive semidwarf mutants of Arabidopsis

Author:
Sponsel, V.M.; Schmidt, F.W.; Porter, S.G.; Nakayama, M.; Kohlstruk, S.; Estelle, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1009-1020
ISSN:
0032-0889
Subject:
height; genes; phenotype; stems; gene expression; oxidation; dwarf cultivars; Arabidopsis thaliana; quantitative analysis; genetic code; plant characteristics; gibberellins; enzyme activity; biosynthesis; loci; genetic variation; biochemical pathways; oxidoreductases; mutants; chemical constituents of plants
Abstract:
... Chemical mutagenesis of Arabidopsis thaliana (L.) Heynh. yielded four semidwarf mutants, all of which appeared to be gibberellin (GA)-biosynthesis mutants. All four had atypical response profiles to C20-GAs, suggesting that each had impaired 20-oxidation. One mutant, 11.2, was shown to be allelic to ga5 and has been named ga5-2. It had altered metabolism of [14C]GA15 relative to that in wild-type ...
PubMed:
9390435
PubMed Central:
PMC158564

5. Characterization of the variation potential in sunflower

Author:
Stankovic, B.; Zawadzki, T.; Davies, E.
Source:
Plant physiology 1997 v.115 no.3 pp. 1083-1088
ISSN:
0032-0889
Subject:
Helianthus annuus; xylem; membrane potential; turgor; proton pump; plasma membrane; ion exchange; stress response
Abstract:
... A major candidate for intercellular signaling in higher plants is the stimulus-induced systemic change in membrane potential known as variation potential (VP). We investigated the mechanism of occurrence and long-distance propagation of VP in sunflower (Helianthus annuus L.) plants. Here we present evidence of the relationship among injury-induced changes in xylem tension, turgor pressure, and ele ...

6. Differential patterns of expression of the Arabidopsis PHYB, PHYD, and PHYE phytochrome genes

Author:
Goosey, L.; Palecanda, L.; Sharrock, R.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 959-969
ISSN:
0032-0889
Subject:
root tips; seedling growth; developmental stages; promoter regions; photoreceptors; phenotype; gene expression; phytochrome; leaves; Arabidopsis thaliana; morphogenesis; amino acid sequences; enzyme activity; apoproteins; transgenic plants; flowers; mutants; reporter genes; genetic variation; plant morphology; beta-glucuronidase; light
Abstract:
... The Arabidopsis thaliana phyB, phyD, and phyE phytochrome apoproteins show higher amino acid sequence similarity to each other than to phyA or phyC, they are the most recently evolved members of this photoreceptor family, and they may interact in regulating photomorphogenesis. The expression patterns of translational fusions of the 5' upstream regions of the PHYB, PHYD, and PHYE genes to the beta- ...
PubMed:
9390432
PubMed Central:
PMC158559

7. Elevated CO2 effects during leaf ontogeny: a new perspective on acclimation

Author:
Miller, A.; Tsai, C.H.; Hemphill, D.; Endres, M.; Rodermel, S.; Spalding, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1195-1200
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; developmental stages; net assimilation rate; acclimation; ontogeny; Nicotiana tabacum; photosynthesis; enzyme activity; leaves
Abstract:
... For many plants growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role that leaf ontogeny plays in the acclimation response, we monitored photosynthesis and some related parameters at short intervals throughout the ontogenetic development of tobacco (Nicotiana tabacum L.) leaves under ambient (350 microliters L-1)- and high (950 microliters L-1)-CO2 conditions. The pa ...

8. Expressing an RbcS antisense gene in transgenic Flaveria bidentis leads to an increased quantum requirement for CO2 fixed in photosystems I and II

Author:
Siebke, K.; Caemmerer, S. von.; Badger, M.; Furbank, R.T.
Source:
Plant physiology 1997 v.115 no.3 pp. 1163-1174
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; carbon dioxide; mathematical models; protein content; gas exchange; transgenic plants; equations; net assimilation rate; light intensity; adenosine triphosphate; electron transfer; gene expression; photosystem II; photosystem I; genetic variation; measurement; enzyme activity; leaves; Flaveria bidentis
Abstract:
... It was previously shown with concurrent measurements of gas exchange and carbon isotope discrimination that the reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase by an antisense gene construct in transgenic Flaveria bidentis (a C4 species) leads to reduced CO2 assimilation rates, increased bundle-sheath CO2 concentration, and leakiness (defined as the ratio of CO2 leakage to the rate of ...

9. Expression of a vacuolar protein (VP24) in anthocyanin-producing cells of sweet potato in suspension culture

Author:
Nozue, M.; Yamada, K.; Nakamura, T.; Kubo, H.; Kondo, M.; Nishimura, M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1065-1072
ISSN:
0032-0889
Subject:
Ipomoea batatas; cell suspension culture; plant proteins; protein synthesis; light; vacuoles; tonoplast; anthocyanins; biosynthesis; immunocytochemistry; cell lines; genetic variation
Abstract:
... VP24, an abundant protein of 24 kD, was found to accumulate in the anthocyanin-containing vacuoles of cells of sweet potato (Ipomoea batatas) in suspension culture. Light-induced expression of VP24 was analyzed by immunoblotting in three different cell lines that produced anthocyanins at different rates. The expression of VP24 was closely correlated with the accumulation of anthocyanin in these ce ...
PubMed:
9390439
PubMed Central:
PMC158570

10. Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane

Author:
Yan, X.H.; Gonzales, R.A.; Wagner, G.J.
Source:
Plant physiology 1997 v.115 no.3 pp. 915-924
ISSN:
0032-0889
Subject:
Phaseolus vulgaris; Manduca sexta; Nicotiana tabacum; leaves; callus; endoplasmic reticulum; sucrose; biochemical pathways; protein composition; beta-glucuronidase; enzyme activity; reporter genes; transgenic plants; gene expression; cytosol; gene transfer; genetic vectors; genetic transformation; plasma membrane; translocation (plant physiology); plant secretions and exudates
Abstract:
... Signal sequences and endoplasmic reticulum (ER)retention signals are known to play central roles in targeting and translocation in the secretory pathway, but molecular aspects about their involvement are poorly understood. We tested the effectiveness of deduced signal sequences from various genes (hydroxyproline-rich glycoprotein [HRGP] from Phaseolus vulgaris; Serpin from Manduca sexta) to direct ...
PubMed:
9390428
PubMed Central:
PMC158555

11. Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L

Author:
Sheveleva, E.; Chmara, W.; Bohnert, H.J.; Jensen, R.G.
Source:
Plant physiology 1997 v.115 no.3 pp. 1211-1219
ISSN:
0032-0889
Subject:
chemical constituents of plants; drought tolerance; drought; measurement; metabolites; glucose; proline; Nicotiana tabacum; fructose; photosynthesis; quantitative analysis; complementary DNA; sucrose; myo-inositol; net assimilation rate; enzyme activity; salt tolerance; salinity; transgenic plants; stress response; diurnal variation; genetic variation; stomata; transferases; leaf conductance
Abstract:
... A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 micromoles g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. ...

12. LHT1, a lysine- and histidine-specific amino acid transporter in Arabidopsis

Author:
Chen, L.S.; Bush, D.R.
Source:
Plant physiology 1997 v.115 no.3 pp. 1127-1134
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; roots; leaves; flowers; pollen; cell membranes; protein composition; lysine; histidine; arginine; active transport; gene expression; complementary DNA; genetic code; amino acid sequences
Abstract:
... We have identified a new amino acid transporter from the Arabidopsis thaliana expressed sequence tag cDNA collection by functional complementation of a yeast amino acid transport mutant. Transport analysis of the expressed protein in yeast shows that it is a high-affinity transporter for both lysine (Lys) and histidine with Michaelis constant values of 175 and 400 micromolar, respectively. This tr ...
PubMed:
9390441
PubMed Central:
PMC158577

13. Membrane fluidity and temperature perception

Author:
Murata, N.; Los, D.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 875-879
ISSN:
0032-0889
Subject:
enzyme activity; unsaturated fatty acids; stress response; acclimation; translocation (plant physiology); gene expression; cell membranes; genes; temperature; Cyanobacteria; stearoyl-CoA desaturase; permeability

14. Occam's razor applied to hormonology: are cytokinins produced by plants?

Author:
Holland, M.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 865-868
ISSN:
0032-0889
Subject:
plant physiology; roots; shoots; tissue culture; cytokinins; quantitative analysis; cell growth; metabolism; genes; chemical constituents of plants

15. RNase activities are reduced concomitantly with conservation of total cellular RNA and ribosomes in O2-deprived seedling roots of maize

Author:
Fennoy, S.L.; Jayachandran, S.; Bailey-Serres, J.
Source:
Plant physiology 1997 v.115 no.3 pp. 1109-1117
ISSN:
0032-0889
Subject:
Zea mays; seedlings; roots; anaerobic conditions; oxygen; ribonucleases; isozymes; enzyme activity; ribosomes; cytosol; pH; aerobic conditions; stress response
Abstract:
... The effect of O2 deprivation on the activities of RNases and levels of total cellular RNA and ribosomes in seedling roots of maize (Zea mays L.) was investigated. Sodium dodecyl sulfate-polyacrylamide gels containing RNA were used to distinguish RNase isoenzymes by apparent molecular mass. Since O2 deprivation causes a decrease in cytosolic pH from approximately pH 7.4 to 6.4 and an elevation in c ...

16. Rice hemoglobins: gene cloning, analysis, and O2-binding kinetics of a recombinant protein synthesized in Escherichia coli

Author:
Arredondo-Peter, R.; Hargrove, M.S.; Sarath, G.; Moran, J.F.; Lohrman, J.; Olson, J.S.; Klucas, R.V.
Source:
Plant physiology 1997 v.115 no.3 pp. 1259-1266
ISSN:
0032-0889
Subject:
Oryza sativa; genes; genetic code; hemoglobin; Escherichia coli; gene transfer; gene expression; roots; leaves; recombinant proteins; oxygen; diffusion; kinetics; symbiosis; mutants; nucleotide sequences; amino acid sequences; complementary DNA; binding sites
Abstract:
... Although nonsymbiotic hemoglobins (Hbs) are found in different tissues of dicots and monocots, very little is known about hb genes in monocots and the function of Hbs in nonsymbiotic tissues. We report the cloning and analysis of two rice (Oryza sativa L.) hb genes, hb1 and hb2, that code for plant Hbs. Rice hb1 and hb2 genes contain four exons and three introns, as with all of the known plant hb ...
PubMed:
9390447
PubMed Central:
PMC158590

17. Root-zone salinity alters raffinose oligosaccharide metabolism and transport in coleus

Author:
Gilbert, G.A.; Wilson, C.; Madore, M.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 1267-1276
ISSN:
0032-0889
Subject:
chemical reactions; raffinose; roots; metabolites; source-sink relationships; carbohydrate metabolism; leaves; nutrient transport; galactans; quantitative analysis; sucrose; myo-inositol; net assimilation rate; phloem; enzyme activity; biosynthesis; salinity; stress response; biochemical pathways; chemical constituents of plants; transferases; Plectranthus scutellarioides
Abstract:
... Exposure of variegated coleus (Coleus blumei Benth.) plants to a saline root-zone environment (60 mM NaCl:12 mM CaCl2) resulted in a significant decline in elongation growth rate over the 30-d experimental period. During the initial 5 to 10 d of exposure, mature source leaves showed strongly diminished rates of photosynthesis, which gradually recovered to close to the control rates by the end of t ...

18. Sorption of aluminum to plasma membrane vesicles isolated from roots of Scout 66 and Atlas 66 cultivars of wheat

Author:
Yermiyahu, U.; Brauer, D.K.; Kinraide, T.B.
Source:
Plant physiology 1997 v.115 no.3 pp. 1119-1125
ISSN:
0032-0889
Subject:
Triticum aestivum; roots; aluminum; phytotoxicity; ion transport; cations; anions; measurement; membrane potential; cultivars; genetic variation; plasma membrane; electron transfer; stress response
Abstract:
... To further elucidate the mechanisms of differential genotypic tolerance to Al, plasma membrane (PM) vesicles were isolated from whole roots, root tips, and tipless roots of Al3+-sensitive and Al3+-tolerantcultivars (cv) of wheat (Triticum aestivum L. cv Scout 66 and cv Atlas 66, respectively). Vesicles from cv Scout root tips sorbed more Al than vesicles prepared from any other source. The intrins ...

19. Substrate kinetics of the plant mitochondrial alternative oxidase and the effects of pyruvate

Author:
Hoefnagel, M.H.N.; Rich, P.R.; Zhang, Q.; Wiskich, J.T.
Source:
Plant physiology 1997 v.115 no.3 pp. 1145-1153
ISSN:
0032-0889
Subject:
inflorescences; Glycine max; dose response; pyruvic acid; cotyledons; oxidoreductases; ubiquinones; mitochondria; oxidation; Arum italicum; kinetics; enzyme activity; protein composition; measurement
Abstract:
... The kinetics of alternative oxidase (AOX) of Arum italicum spadices and soybean (Glycine max L.) cotyledons were studied both with intact mitochondria and with a solubilized, partially purified enzyme. Ubiquinone analogs were screened for their suitability as substrates and ubiquinol-1 was found to be most suitable. The kinetics of ubiquinol-1 oxidation via AOX in both systems followed Michaelis-M ...

20. Treatment of grape berries, a nonclimacteric fruit with a synthetic auxin, retards ripening and alters the expression of developmentally regulated genes

Author:
Davies, C.; Boss, P.K.; Robinson, S.P.
Source:
Plant physiology 1997 v.115 no.3 pp. 1155-1161
ISSN:
0032-0889
Subject:
developmental stages; genes; gene expression; Vitis vinifera; hexoses; anthocyanins; weight; plant development; quantitative analysis; naringenin-chalcone synthase; beta-fructofuranosidase; growth retardation; enzyme activity; acetic acid; biosynthesis; abscisic acid; ripening; color; chemical constituents of plants; chitinase
Abstract:
... Treatment of grape (Vitis vinifera L.) berries with the synthetic auxin-like compound benzothiazole-2-oxyacetic acid (BTOA) caused a delay in the onset of ripening of approximately 2 weeks. This was manifested as a retardation of the increases in berry weight, color, deformability, and hexose concentration. BTOA treatment also delayed by 2 weeks the increase in abscisic acid level that normally ac ...