Jump to Main Content
PubAg
Main content area
Search
Search Results
- Author:
- Korth, K.L.; Dixon, R.A.
- Source:
- Plant physiology 1997 v.115 no.4 pp. 1299-1305
- ISSN:
- 0032-0889
- Subject:
- insect pests; biosynthesis; Manduca sexta; Solanum tuberosum; transcription (genetics); genetic markers; mechanical damage; genes; proteinase inhibitors; abiotic stress; pest resistance; hydroxymethylglutaryl-CoA reductases; leaves; defense mechanisms
- Abstract:
- ... The timing of transcript accumulation of several wound-induced genes is different in insect-damaged and mechanically damaged leaves. Transcripts for the proteinase inhibitor II and 3-hydroxy-3-methylglutaryl-coenzyme A reductase genes accumulate more rapidly in potato (Solanum tuberosum L.) leaves chewed on by caterpillars than in leaves damaged mechanically. The timing of maximum transcript accum ...
-
- Author:
- Lejay, L.; Quillere, I.; Roux, Y.R.; Tillard, P.; Cliquet, J.B.; Meyer, C.; Morot-Gaudry, J.F.; Gojon, A.
- Source:
- Plant physiology 1997 v.115 no.2 pp. 623-631
- ISSN:
- 0032-0889
- Subject:
- Nicotiana plumbaginifolia; leaves; nitrate reduction; nitrate reductase; enzyme activity; light; photosynthesis; roots; free amino acids; transgenic plants; complementary DNA; gene expression; genetic variation; transcription (genetics); chemical constituents of plants; genotype
- Abstract:
- ... The activity of nitrate reductase (NR) in leaves is regulated by light and photosynthesis at transcriptional and posttranscriptional levels. To understand the physiological role of these controls, we have investigated the effects of light and CO2 on in vivo NO3- reduction in transgenic plants of Nicotiana plumbaginifolia lacking either transcriptional regulation alone or transcriptional and post-t ...
-
- Author:
- Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
- Source:
- Plant physiology 1997 v.115 no.2 pp. 397-407
- ISSN:
- 0032-0889
- Subject:
- protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
- Abstract:
- ... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...
-
- Author:
- Doran, E.; Cattolico, R.A.
- Source:
- Plant physiology 1997 v.115 no.2 pp. 773-781
- ISSN:
- 0032-0889
- Subject:
- chloroplasts; plant development; cell growth; complementary DNA; algae; cell division; transcription (genetics); gene expression; genes; nucleotide sequences; photoperiod; photosynthesis; RNA; light
- Abstract:
- ... Light acts as a complex regulator of cellular development and gene expression in photoautotrophs. Although light signals are highly effective in controlling cellular division and chloroplast biogenesis in the toxic marine alga Heterosigma carterae, their influence on gene expression has not been well characterized. To address this need cultures of H. carterae synchronized by an alternating light-d ...
-
- Author:
- Thipyapong, P.; Steffens, J.C.
- Source:
- Plant physiology 1997 v.115 no.2 pp. 409-418
- ISSN:
- 0032-0889
- Subject:
- plant pathogenic bacteria; ethylene; developmental stages; plant pathogenic fungi; salicylic acid; cell differentiation; promoter regions; stems; jasmonic acid; gene expression; mechanical damage; disease resistance; leaves; genetic code; catechol oxidase; transcription (genetics); abiotic stress; enzyme activity; Solanum lycopersicum var. lycopersicum; stress response; plant morphology
- Abstract:
- ... Tomato (Lycopersicon esculentum Mill.) polyphenol oxidases (PPOs) are encoded by a seven-member gene family that exhibits complex patterns of differential expression during growth and differentiation. Antisense down-regulation of constitutive and induced PPO expression results in hypersusceptibility to pathogens, suggesting a critical role for PPO-mediated phenolic oxidation in plant defense. Howe ...
-
- Author:
- Lagrimini, L.M.; Gingas, V.; Finger, F.; Rothstein, S.; Liu, T.T.Y.
- Source:
- Plant physiology 1997 v.114 no.4 pp. 1187-1196
- ISSN:
- 0032-0889
- Subject:
- cell growth; chemical constituents of plants; Nicotiana sylvestris; developmental stages; lignification; genes; roots; antisense RNA; phenotype; stems; cell walls; Nicotiana tabacum; genotype; peroxidase; leaves; defense mechanisms; genetic code; morphogenesis; transcription (genetics); xylem; lignin; enzyme activity; isozymes; transgenic plants; stress response; genetic variation; plant morphology
- Abstract:
- ... On the basis of the biological compoundsthat they metabolize, plant peroxidases have long been implicated in plant growth, cell wall biogenesis, lignification, and host defenses. Transgenic tobacco (Nicotiana tabacum L.) plants that underexpress anionic peroxidase were generated using antisense RNA. The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing ...
-
- Author:
- Nie, X.; Hill, R.D.
- Source:
- Plant physiology 1997 v.114 no.3 pp. 835-840
- ISSN:
- 0032-0889
- Subject:
- oligomycin; messenger RNA; anaerobic conditions; Hordeum vulgare; oxygen consumption; hemoglobin; antimycin A; adenosine triphosphate; gene expression; aleurone cells; transcription (genetics); mitochondria; carbon monoxide; adenosinetriphosphatase; 2,4-dinitrophenol; cyanides
- Abstract:
- ... Previous studies have shown that plant hemoglobin (Hb) mRNA is expressed in barley (Hordeum vulgare L.) aleurone layers during hypoxia. We have examined the effect of a number of respiratory inhibitors onbarley aleurone layers to determine the factors that influence Hb gene expression. Respiratory inhibitors that reduce O2 consumption, such as CO, cyanide, and antimycin A, strongly enhanced Hb mRN ...
-
- Author:
- Gordon, A.J.; Minchin, F.R.; Skot, L.; James, C.L.
- Source:
- Plant physiology 1997 v.114 no.3 pp. 937-946
- ISSN:
- 0032-0889
- Subject:
- gas exchange; glutamate-ammonia ligase; drought; shoot meristems; sucrose synthase; nitrogen fixation; ureides; starch; peroxidases; gene expression; leghemoglobin; oxygen; metabolism; Glycine max; messenger RNA; quantitative analysis; glutamine; sucrose; cutting; root nodules; transcription (genetics); sodium chloride; enzyme activity; ascorbic acid; glutaric acid; stress response; chemical constituents of plants; nitrogenase; nitrates
- Abstract:
- ... Soybean (Glycine max L.) plants were subjected to a number of treatments (drought, 10 mM nitrate, 150 mM NaCl, shoot meristem removal, and removal of approximately 50% of the nodules) to test the hypothesis that metabolic responses contribute to the regulation of N2 fixation. Nitrogenase activity was correlated with the activity of nodule sucrose synthase (SS), but not with that of glutamine oxogl ...
-
- Author:
- Gary, G.R.; Chauvin, L.P.; Sarhan, F.; Huner, N.P.A.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 467-474
- ISSN:
- 0032-0889
- Subject:
- messenger RNA; dose response; redox reactions; net assimilation rate; light intensity; acclimation; Secale cereale; gene expression; transcription (genetics); frost resistance; photosynthesis; genes; temperature; plant morphology; chloroplasts
- Abstract:
- ... By comparing growth under five different temperature and irradiance regimes (20 degrees C and 800, 250, and 50 micromoles m-2 s-1 and 5 degrees C and 250 and 50 micromoles m-2 s-1), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal temperature at which freezing injury occurs [LT50]), transcript lev ...
-
- Author:
- Sivasankar, S.; Rothstein, S.; Oaks, A.
- Source:
- Plant physiology 1997 v.114 no.2 pp. 583-589
- ISSN:
- 0032-0889
- Subject:
- Zea mays; seedlings; roots; shoots; nitrate reduction; nitrogen; carbon; metabolites; asparagine; glutamine; sucrose; dose response; nutrient uptake; nitrate reductase; enzyme activity; nitrite reductase; aspartate-ammonia ligase; genes; gene expression; transcription (genetics)
- Abstract:
- ... The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After 8 h of exposure to nitrate in the presence of 1 mM Asn, tissue nitrate accumulati ...
-
- Author:
- Gao, M.; Fisher, D.K.; Kim, K.N.; Shannon, J.C.; Guiltinan, M.J.
- Source:
- Plant physiology 1997 v.114 no.1 pp. 69-78
- ISSN:
- 0032-0889
- Subject:
- Zea mays; amylopectin; biosynthesis; branching; isozymes; enzyme activity; endosperm; embryo (plant); roots; leaves; complementary DNA; genetic code; nucleotide sequences; amino acid sequences; messenger RNA; structural genes; gene expression; chemical constituents of plants; transcription (genetics)
- Abstract:
- ... In maize (Zea mays L.) three isoforms of starch-branching enzyme (SBEI, SBEIIa, and SBEIIb) are involved in the synthesis of amylopectin, the branched component of starch. To isolate a cDNA encoding SBEIIa, degenerate oligonucleotides based on domains highly conserved in Sbe2 family members were used to amplify Sbe2-family cDNA from tissues lacking SBEIIb activity. The predicted amino acid sequenc ...
-
- Author:
- Jones, T.L.; Ort, D.R.
- Source:
- Plant physiology 1997 v.113 no.4 pp. 1167-1175
- ISSN:
- 0032-0889
- Subject:
- hexosyltransferases; protein synthesis; biosynthesis; cycloheximide; cordycepin; phosphoric monoester hydrolases; sucrose; transcription (genetics); enzyme inhibitors; gene expression; diurnal variation; genes; okadaic acid; phosphotransferases (kinases); phosphorylation; enzyme activity; circadian rhythm; light; Solanum lycopersicum var. lycopersicum
- Abstract:
- ... Sucrose phosphate synthase (SPS), a key enzyme in sucrose biosynthesis, is regulated by protein phosphorylation and shows a circadian pattern of activity in tomato. SPS is most active in its dephosphorylated state, which normally coincides with daytime. Applying okadaic acid, a potent protein phosphatase inhibitor, prevents SPS activation. More interesting is that a brief treatment with cyclohexim ...
-
- Author:
- Cushman, J.C.; Bohnert, H.J.
- Source:
- Plant physiology 1997 v.113 no.3 pp. 667-676
- ISSN:
- 0032-0889
- Subject:
- ribulose-bisphosphate carboxylase; carboxylation; photorespiration; phosphoenolpyruvate carboxylase; Crassulacean acid metabolism; gene expression; transcription (genetics); genes; plant physiology; enzyme activity
- Abstract:
- ... Most higher plants assimilate atmospheric CO2 through the C3 pathway of photosynthesis using ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). However, when CO2 availability is reduced by environmental stress conditions, the incomplete discrimination of CO2 over O2 by Rubisco leads to increased photorespiration, a process that reduces the efficiency of C3 photosynthesis. To overcome the w ...
-
- Author:
- Batard, Y.; Schalk, M.; Pierrel, M.A.; Zimmerlin, A.; Durst, F.; Werck-Reichhart, D.
- Source:
- Plant physiology 1997 v.113 no.3 pp. 951-959
- ISSN:
- 0032-0889
- Subject:
- protein synthesis; dose response; phytochrome; genetic code; oxygenases; stress response; phenobarbital; cinnamic acid; tubers; gene expression; transcription (genetics); genes; clofibrate; enzyme activity; chemical treatment; benzopyrenes; Helianthus tuberosus
- Abstract:
- ... trans-Cinnamate 4-hydroxylase (C4H) is a plant-specific cytochrome (P450) that is encoded by the gene CYPZ3A and catalyzes the second step of the multibranched phenylpropanoid pathway. Increases in C4H activity in response to physical and chemical stresses have been well documented, but the mechanism of these increases has never been studied in detail. This paper reports on the regulatory mechanis ...
-
- Author:
- Donahue, J.L.; Okpodu, C.M.; Cramer, C.L.; Grabau, E.A.; Alscher, R.G.
- Source:
- Plant physiology 1997 v.113 no.1 pp. 249-257
- ISSN:
- 0032-0889
- Subject:
- developmental stages; cytosol; Pisum sativum; oxidation; photosynthesis; peroxidase; leaves; cultivars; messenger RNA; superoxide dismutase; net assimilation rate; transcription (genetics); herbicide resistance; enzyme activity; antioxidants; ascorbic acid; glutathione-disulfide reductase; phytotoxicity; stress response; plastids; paraquat; genetic variation
- Abstract:
- ... Differential sensitivity to the oxidant paraquat was observed in pea (Pisum sativum L.) based on cultivar and leaf age. To assess contributions of inductive responses of the antioxidant enzymes in short-term resistance to oxidative damage, activities of glutathione reductase (GR), superoxide dismutase (SOD), and ascorbate peroxidase (APX) and transcript levels for plastidic GR, Cu,Zn SOD, and cyto ...
-